Standard name | |
---|---|
Human Ortholog | |
Description | Small subunit of the clathrin-associated adaptor complex AP-2; AP-2 is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 70 | 18 | 35 | 19 | 57 | 20 | 25 | 22 | 30 | 21 | 36 | 62 | 101 | 116 | 45 | 22 | 39 | 25 | 65 | 30 | 32 | 142 | 151 |
Bud | 4 | 20 | 3 | 24 | 49 | 54 | 75 | 75 | 66 | 78 | 66 | 94 | 23 | 27 | 30 | 10 | 3 | 8 | 10 | 30 | 5 | 16 | 72 | 120 |
Bud Neck | 1 | 10 | 1 | 11 | 20 | 14 | 13 | 12 | 5 | 6 | 2 | 7 | 40 | 64 | 92 | 9 | 6 | 11 | 24 | 84 | 22 | 87 | 213 | 204 |
Bud Site | 7 | 33 | 4 | 22 | 69 | 59 | 49 | 52 | 46 | 46 | 33 | 40 | 13 | 30 | 16 | 14 | 10 | 11 | – | – | – | – | – | – |
Cell Periphery | 2 | 13 | 4 | 24 | 14 | 16 | 35 | 35 | 23 | 38 | 24 | 47 | 30 | 38 | 57 | 13 | 4 | 10 | 8 | 9 | 6 | 12 | 44 | 50 |
Cytoplasm | 23 | 61 | 9 | 90 | 90 | 102 | 164 | 164 | 107 | 114 | 75 | 158 | 358 | 436 | 512 | 27 | 21 | 34 | 10 | 17 | 4 | 19 | 44 | 47 |
Endoplasmic Reticulum | 0 | 11 | 0 | 6 | 6 | 19 | 19 | 12 | 6 | 17 | 3 | 24 | 5 | 14 | 21 | 3 | 8 | 8 | 0 | 2 | 2 | 2 | 7 | 9 |
Endosome | 0 | 0 | 0 | 2 | 47 | 0 | 3 | 2 | 6 | 7 | 5 | 5 | 0 | 1 | 3 | 2 | 1 | 0 | 1 | 2 | 1 | 3 | 6 | 7 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 4 |
Mitochondria | 3 | 1 | 7 | 20 | 63 | 146 | 215 | 215 | 216 | 252 | 206 | 316 | 0 | 0 | 1 | 10 | 3 | 7 | 5 | 5 | 3 | 16 | 37 | 39 |
Nucleus | 0 | 4 | 0 | 1 | 13 | 4 | 5 | 5 | 21 | 15 | 14 | 2 | 0 | 3 | 1 | 2 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 1 | 1 | 0 | 4 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 1 | 1 | 5 | 1 | 8 | 8 | 6 | 10 | 9 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 6 | 4 | 0 | 28 | 83 | 25 | 39 | 38 | 33 | 43 | 22 | 42 | 0 | 2 | 1 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 10 | 7 | 1 | 18 | 44 | 9 | 21 | 22 | 23 | 28 | 22 | 27 | 31 | 82 | 92 | 13 | 9 | 11 | 1 | 0 | 0 | 1 | 0 | 4 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 5 | 0 | 7 | 6 | 4 | 14 | 12 | 2 | 0 | 5 | 1 | 1 | 1 | 0 | 2 | 4 | 1 | 6 | 32 | 29 |
Unique Cell Count | 48 | 165 | 35 | 201 | 363 | 334 | 489 | 488 | 400 | 488 | 336 | 562 | 455 | 628 | 740 | 106 | 61 | 93 | 92 | 225 | 82 | 203 | 609 | 684 |
Labelled Cell Count | 65 | 234 | 49 | 284 | 528 | 506 | 677 | 673 | 584 | 698 | 515 | 807 | 562 | 804 | 945 | 153 | 91 | 145 | 92 | 225 | 82 | 203 | 609 | 684 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 5.6 | 4.7 | 5.0 | 4.6 | 4.3 | 3.9 | 3.9 | 4.2 | 4.2 | 4.2 | 4.2 | 5.7 | 5.6 | 5.7 | 6.2 | 6.5 | 6.6 | 6.2 | 6.0 | 6.3 |
Std Deviation (1e-4) | 0.6 | 0.7 | 1.1 | 1.8 | 1.7 | 1.5 | 1.4 | 1.4 | 1.4 | 1.0 | 1.0 | 1.0 | 0.8 | 1.0 | 0.8 | 1.2 | 1.3 | 1.2 | 1.0 | 1.1 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.06 | -0.03 | -0.14 | -0.29 | -0.29 | -0.16 | -0.16 | -0.19 | -0.17 | 0.27 | 0.25 | 0.26 | 0.39 | 0.46 | 0.47 | 0.38 | 0.33 | 0.41 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -4.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.8 | -5.3 | -5.5 | -0.9 | -1.5 | -1.0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.1 | -0.1 | 0.6 | 1.0 | 1.0 | 0.1 | -0.3 | -0.5 | 0.3 | 7.0 | 5.4 | 5.4 | 0 | 0.9 | 1.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.8115 | 1.6656 | 1.584 | 1.4106 | 2.4906 | 1.5406 | 1.2427 | 1.1326 | 1.4613 | 1.5196 | 1.3751 | 1.5538 | -0.352 | -0.0645 | -0.1592 | -0.2049 | -0.3161 | -0.043 |
Actin | 0.1259 | 0.0327 | 0.0301 | 0.0075 | 0.0779 | 0.0747 | 0.0931 | 0.0229 | 0.0336 | 0.0173 | 0.064 | 0.0655 | 0.1622 | 0.0661 | 0.0338 | 0.0042 | 0.0549 | 0.0857 |
Bud | 0.0064 | 0.0068 | 0.0019 | 0.0005 | 0.0022 | 0.0032 | 0.0029 | 0.0039 | 0.0013 | 0.0004 | 0.002 | 0.0018 | 0.0026 | 0.0034 | 0.002 | 0.0006 | 0.002 | 0.0038 |
Bud Neck | 0.1415 | 0.0322 | 0.589 | 0.9436 | 0.5342 | 0.5281 | 0.1036 | 0.044 | 0.6274 | 0.818 | 0.6119 | 0.6714 | 0.1592 | 0.0408 | 0.6289 | 0.8971 | 0.6954 | 0.5912 |
Bud Periphery | 0.0308 | 0.0141 | 0.0061 | 0.0027 | 0.019 | 0.0226 | 0.0208 | 0.0104 | 0.0048 | 0.0027 | 0.0131 | 0.0088 | 0.008 | 0.0081 | 0.0064 | 0.0028 | 0.0089 | 0.0365 |
Bud Site | 0.2867 | 0.5671 | 0.2504 | 0.0058 | 0.0507 | 0.0204 | 0.4139 | 0.6191 | 0.2286 | 0.0074 | 0.01 | 0.0071 | 0.3914 | 0.6332 | 0.2455 | 0.023 | 0.0215 | 0.0115 |
Cell Periphery | 0.0873 | 0.031 | 0.017 | 0.0072 | 0.0565 | 0.047 | 0.0701 | 0.0253 | 0.0134 | 0.0154 | 0.0193 | 0.0123 | 0.0523 | 0.0209 | 0.0058 | 0.0144 | 0.0224 | 0.0408 |
Cytoplasm | 0.1972 | 0.2314 | 0.0501 | 0.0298 | 0.148 | 0.233 | 0.1401 | 0.1218 | 0.0224 | 0.0212 | 0.164 | 0.1065 | 0.1046 | 0.1066 | 0.0148 | 0.0528 | 0.1222 | 0.1597 |
Cytoplasmic Foci | 0.0133 | 0.0039 | 0.0039 | 0.001 | 0.0017 | 0.0099 | 0.0087 | 0.0028 | 0.0024 | 0.0107 | 0.0044 | 0.005 | 0.0177 | 0.0045 | 0.0027 | 0.0011 | 0.0032 | 0.0107 |
Eisosomes | 0.003 | 0.0016 | 0.0011 | 0.0001 | 0.0016 | 0.0019 | 0.0087 | 0.0023 | 0.0012 | 0.0017 | 0.0016 | 0.0012 | 0.0046 | 0.0038 | 0.0008 | 0 | 0.0005 | 0.0009 |
Endoplasmic Reticulum | 0.006 | 0.0018 | 0.0003 | 0 | 0.0035 | 0.0019 | 0.0022 | 0.0051 | 0.0043 | 0.0002 | 0.0016 | 0.0011 | 0.0032 | 0.0023 | 0.0001 | 0.0013 | 0.0019 | 0.0012 |
Endosome | 0.0064 | 0.0005 | 0.0002 | 0.0001 | 0.0034 | 0.0009 | 0.0016 | 0.0003 | 0.0005 | 0.0001 | 0.0002 | 0.0006 | 0.0029 | 0.0008 | 0.0003 | 0.0002 | 0.0003 | 0.0018 |
Golgi | 0.0045 | 0.0009 | 0.0004 | 0.0002 | 0.0051 | 0.0014 | 0.0024 | 0.0005 | 0.0005 | 0.0003 | 0.0006 | 0.001 | 0.0028 | 0.0011 | 0.0006 | 0.0003 | 0.0006 | 0.0021 |
Lipid Particles | 0.0052 | 0.0004 | 0.0001 | 0 | 0.0025 | 0.0007 | 0.0026 | 0.0007 | 0.0008 | 0.0006 | 0.0002 | 0.0013 | 0.0027 | 0.0055 | 0.0002 | 0.0002 | 0.0004 | 0.0012 |
Mitochondria | 0.0015 | 0.0003 | 0.0001 | 0 | 0.001 | 0.0004 | 0.0004 | 0.0002 | 0.0006 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0004 | 0.0003 | 0 | 0.0001 | 0.0018 |
None | 0.0621 | 0.0715 | 0.047 | 0.0006 | 0.0849 | 0.0487 | 0.1161 | 0.138 | 0.0513 | 0.0979 | 0.1055 | 0.1128 | 0.079 | 0.0982 | 0.0559 | 0.0002 | 0.0628 | 0.044 |
Nuclear Periphery | 0.0028 | 0.0003 | 0 | 0 | 0.0038 | 0.0002 | 0.0011 | 0.0002 | 0.0011 | 0 | 0.0001 | 0.0002 | 0.0004 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0021 |
Nucleolus | 0.0012 | 0.0006 | 0.0004 | 0.0001 | 0.0003 | 0.0004 | 0.0017 | 0.0002 | 0.0006 | 0.0012 | 0.0001 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0003 |
Nucleus | 0.0016 | 0.0011 | 0.0005 | 0.0002 | 0.0007 | 0.0007 | 0.0014 | 0.0004 | 0.0006 | 0.0006 | 0.0003 | 0.0003 | 0.0007 | 0.0006 | 0.0003 | 0.0003 | 0.0006 | 0.0007 |
Peroxisomes | 0.0119 | 0.0004 | 0.0003 | 0 | 0.0005 | 0.0012 | 0.0048 | 0.0003 | 0.0011 | 0.0007 | 0.0002 | 0.0012 | 0.0013 | 0.0008 | 0.0008 | 0 | 0.0002 | 0.0016 |
Punctate Nuclear | 0.0014 | 0.0004 | 0.0002 | 0.0001 | 0.0011 | 0.0004 | 0.0019 | 0.0002 | 0.0003 | 0.003 | 0.0001 | 0.0008 | 0.0023 | 0.0006 | 0.0002 | 0.0001 | 0.0004 | 0.0009 |
Vacuole | 0.003 | 0.0012 | 0.0007 | 0.0003 | 0.0009 | 0.0024 | 0.0017 | 0.0012 | 0.0028 | 0.0003 | 0.0007 | 0.0005 | 0.0013 | 0.0015 | 0.0003 | 0.001 | 0.0013 | 0.0012 |
Vacuole Periphery | 0.0003 | 0.0001 | 0 | 0 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.6089 | 24.4372 | 18.1335 | 31.3692 | 16.1105 | 29.8052 | 31.4472 | 41.6914 | 34.8201 | 34.2198 |
Translational Efficiency | 1.1609 | 1.0813 | 1.5972 | 1.0799 | 2.0381 | 1.3187 | 1.3621 | 0.8471 | 1.2567 | 1.2507 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1420 | 1691 | 1880 | 885 | 1216 | 1663 | 2220 | 1089 | 2636 | 3354 | 4100 | 1974 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 578.72 | 748.01 | 804.86 | 831.63 | 719.06 | 722.55 | 809.20 | 865.62 | 643.46 | 735.39 | 807.21 | 850.38 |
Standard Deviation | 77.25 | 103.29 | 95.12 | 109.20 | 81.64 | 201.37 | 90.55 | 105.72 | 105.76 | 160.15 | 92.70 | 108.61 |
Intensity Change Log 2 | — | 0.370192 | 0.475872 | 0.523076 | — | 0.006985 | 0.170384 | 0.267622 | — | 0.180320 | 0.314648 | 0.387153 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.007241 | 0.026483 | 0.013859 | 0.057844 | 0.015608 | 0.022270 | 0.016579 | 0.039589 | 0.011101 | 0.024394 | 0.015332 | 0.047773 |
Bud Neck | 0.283091* | 0.245278* | 0.246706* | 0.102810 | 0.333673* | 0.331515* | 0.251738* | 0.160209 | 0.306425* | 0.288037* | 0.249431* | 0.134475 |
Bud Site | 0.105534* | 0.263867* | 0.349929* | 0.298903* | 0.226193* | 0.239601* | 0.351871* | 0.283159* | 0.161195* | 0.251835* | 0.350980* | 0.290217* |
Cell Periphery | 0.015157 | 0.004769 | 0.004838 | 0.006882 | 0.007618 | 0.009152 | 0.004219 | 0.007716 | 0.011680 | 0.006942 | 0.004503 | 0.007342 |
Cytoplasm | 0.300394* | 0.217164* | 0.207355* | 0.229673* | 0.258492* | 0.217795* | 0.228145* | 0.256339* | 0.281064* | 0.217477* | 0.218612* | 0.244384* |
Cytoplasmic Foci | 0.106073 | 0.104396 | 0.032102 | 0.042662 | 0.066817 | 0.062754 | 0.017356 | 0.032512 | 0.087964 | 0.083749 | 0.024118 | 0.037063 |
Eisosomes | 0.012851 | 0.005280 | 0.000898 | 0.002849 | 0.006341 | 0.007654 | 0.000734 | 0.002761 | 0.009848 | 0.006457 | 0.000809 | 0.002800 |
Endoplasmic Reticulum | 0.003678 | 0.001706 | 0.002159 | 0.005020 | 0.001679 | 0.000842 | 0.000916 | 0.002322 | 0.002756 | 0.001278 | 0.001486 | 0.003531 |
Endosome | 0.014877 | 0.022149 | 0.005711 | 0.004804 | 0.009474 | 0.014978 | 0.005103 | 0.003957 | 0.012385 | 0.018594 | 0.005381 | 0.004337 |
Golgi | 0.015904 | 0.015528 | 0.001010 | 0.001033 | 0.007209 | 0.012620 | 0.000949 | 0.001212 | 0.011893 | 0.014086 | 0.000977 | 0.001132 |
Lipid Particles | 0.023281 | 0.009047 | 0.001976 | 0.002759 | 0.004498 | 0.004797 | 0.001057 | 0.001664 | 0.014616 | 0.006940 | 0.001478 | 0.002155 |
Mitochondria | 0.039231 | 0.010305 | 0.001466 | 0.000473 | 0.005789 | 0.020253 | 0.001515 | 0.000653 | 0.023804 | 0.015237 | 0.001493 | 0.000572 |
Mitotic Spindle | 0.000243 | 0.001294 | 0.003244 | 0.004904 | 0.000366 | 0.001392 | 0.002914 | 0.001295 | 0.000299 | 0.001343 | 0.003065 | 0.002913 |
None | 0.003777 | 0.000697 | 0.000634 | 0.000596 | 0.000558 | 0.000278 | 0.000878 | 0.000485 | 0.002292 | 0.000489 | 0.000766 | 0.000535 |
Nuclear Periphery | 0.000134 | 0.000057 | 0.000165 | 0.000145 | 0.000034 | 0.000023 | 0.000087 | 0.000077 | 0.000088 | 0.000040 | 0.000123 | 0.000107 |
Nuclear Periphery Foci | 0.000173 | 0.000558 | 0.001192 | 0.001133 | 0.000198 | 0.000096 | 0.000243 | 0.000624 | 0.000184 | 0.000329 | 0.000678 | 0.000852 |
Nucleolus | 0.000359 | 0.000341 | 0.000169 | 0.000099 | 0.000216 | 0.000130 | 0.000148 | 0.000070 | 0.000293 | 0.000236 | 0.000158 | 0.000083 |
Nucleus | 0.030620 | 0.018021 | 0.049616 | 0.040990 | 0.020188 | 0.010673 | 0.038290 | 0.042014 | 0.025808 | 0.014377 | 0.043483 | 0.041555 |
Peroxisomes | 0.020250 | 0.021589 | 0.009744 | 0.018328 | 0.012772 | 0.022548 | 0.010237 | 0.013696 | 0.016801 | 0.022064 | 0.010011 | 0.015773 |
Vacuole | 0.014992 | 0.030089 | 0.067042 | 0.177747 | 0.021636 | 0.019315 | 0.066613 | 0.149517 | 0.018056 | 0.024747 | 0.066809 | 0.162173 |
Vacuole Periphery | 0.002138 | 0.001381 | 0.000186 | 0.000349 | 0.000642 | 0.001314 | 0.000408 | 0.000128 | 0.001448 | 0.001348 | 0.000306 | 0.000227 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -8.42 | -4.55 | -10.66 | -5.08 | -8.79 | -2.47 | -0.21 | -5.90 | -4.22 | -6.56 | -7.68 | -2.82 | -12.17 | -6.47 | -10.91 |
Bud Neck | 3.46 | 4.18 | 19.42 | 18.41 | 18.27 | 0.16 | 8.14 | 16.62 | 18.78 | 11.41 | 2.26 | 8.48 | 24.40 | 25.21 | 20.06 |
Bud Site | -19.91* | -27.50* | -13.81 | 1.03 | 7.31 | -1.40 | -11.52 | -2.40 | -1.31 | 8.05 | -14.50 | -27.37 | -11.81 | -0.12 | 10.88 |
Cell Periphery | 8.04 | 8.56 | 7.38 | -1.24 | -1.57 | -1.77 | 5.28 | 0.86 | 2.55 | -4.06 | 5.86 | 10.03 | 6.65 | 0.95 | -4.16 |
Cytoplasm | 8.94 | 9.17 | 6.00 | -1.71 | -2.19 | 4.10 | 2.48 | -0.66 | -4.82 | -3.22 | 9.34 | 8.30 | 3.71 | -4.84 | -3.90 |
Cytoplasmic Foci | 0.25 | 16.62 | 12.50 | 12.17 | -3.52 | 0.88 | 13.60 | 7.74 | 7.27 | -6.94 | 1.10 | 21.87 | 15.03 | 14.26 | -7.14 |
Eisosomes | 5.68 | 10.26 | 8.56 | 3.80 | -3.98 | -1.47 | 9.45 | 5.81 | 7.05 | -6.06 | 4.09 | 13.16 | 10.20 | 7.63 | -7.05 |
Endoplasmic Reticulum | 2.98 | 1.82 | -1.07 | -3.17 | -2.41 | 2.08 | 1.73 | -1.35 | -3.86 | -3.59 | 3.69 | 2.73 | -1.25 | -4.43 | -3.67 |
Endosome | -3.93 | 7.04 | 7.90 | 11.50 | 1.77 | -4.12 | 4.11 | 5.30 | 10.33 | 1.83 | -5.36 | 8.17 | 9.59 | 15.36 | 2.56 |
Golgi | 0.28 | 14.62 | 14.55 | 13.69 | 0.53 | -4.78 | 8.49 | 7.54 | 11.98 | -0.37 | -2.32 | 16.72 | 15.95 | 18.04 | -0.13 |
Lipid Particles | 6.81 | 11.36 | 10.70 | 5.93 | -1.99 | -0.37 | 8.36 | 6.54 | 4.20 | -3.08 | 6.43 | 12.58 | 11.72 | 7.42 | -3.16 |
Mitochondria | 9.98 | 13.44 | 13.99 | 11.53 | 2.13 | -8.11 | 5.72 | 7.07 | 11.95 | 2.95 | 4.72 | 14.11 | 14.87 | 15.87 | 3.45 |
Mitotic Spindle | -3.14 | -3.78 | -2.68 | -1.99 | -0.63 | -2.00 | -3.94 | -2.15 | 0.00 | 1.93 | -3.42 | -5.48 | -3.27 | -1.79 | 0.35 |
None | 3.77 | 3.83 | 3.51 | 0.13 | -0.01 | 1.51 | -0.68 | 0.24 | -1.01 | 0.79 | 4.01 | 3.02 | 3.55 | -0.32 | 0.64 |
Nuclear Periphery | 3.76 | -1.68 | -1.23 | -3.34 | 0.15 | 3.78 | -8.04 | -7.18 | -8.47 | 0.20 | 4.39 | -3.41 | -2.68 | -5.57 | 0.25 |
Nuclear Periphery Foci | -1.89 | -4.02 | -7.93 | -3.30 | -0.34 | 3.43 | -2.35 | -4.66 | -5.49 | -4.00 | -1.41 | -4.30 | -8.98 | -5.08 | -1.89 |
Nucleolus | 0.01 | 4.25 | 6.66 | 2.97 | 3.12 | 1.47 | 1.08 | 2.31 | 2.43 | 2.34 | 0.95 | 3.35 | 5.78 | 3.49 | 3.69 |
Nucleus | 6.12 | -7.59 | -5.34 | -9.64 | 1.57 | 6.01 | -8.91 | -9.02 | -12.63 | -1.35 | 8.64 | -11.04 | -9.72 | -15.61 | 0.14 |
Peroxisomes | -0.71 | 6.62 | 1.61 | 2.19 | -3.08 | -4.96 | 1.62 | 0.08 | 4.29 | -1.22 | -3.85 | 5.93 | 1.58 | 4.70 | -2.95 |
Vacuole | -8.65 | -18.30 | -25.53 | -23.60 | -17.57 | 1.40 | -17.04 | -23.47 | -23.82 | -14.55 | -5.58 | -25.21 | -34.59 | -33.19 | -22.61 |
Vacuole Periphery | 1.83 | 5.12 | 4.40 | 4.50 | -0.58 | -3.02 | 1.19 | 4.31 | 6.22 | 1.60 | 0.41 | 4.96 | 5.49 | 7.70 | 0.83 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|