Standard name | |
---|---|
Human Ortholog | |
Description | Serine/threonine protein kinase; involved in regulation of ion transport across plasma membrane; carboxyl terminus is essential for glucose-dependent Pma1p activation via phosphorylation of Pma1p-Ser899; enhances spermine uptake; PTK2 has a paralog, PTK1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 3 | 3 | 4 | 2 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 29 | 36 | 37 | 10 | 18 | 15 | 9 | 11 | 13 | 8 | 9 | 14 | 25 | 41 | 27 | 51 | 38 | 23 | 1 | 0 | 0 | 1 | 0 | 2 |
Cytoplasm | 197 | 274 | 162 | 76 | 147 | 89 | 149 | 150 | 110 | 85 | 70 | 120 | 287 | 408 | 468 | 355 | 224 | 205 | 188 | 270 | 163 | 194 | 335 | 343 |
Endoplasmic Reticulum | 0 | 4 | 0 | 0 | 0 | 1 | 2 | 0 | 3 | 2 | 1 | 3 | 3 | 11 | 1 | 12 | 10 | 7 | 2 | 2 | 1 | 25 | 33 | 22 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 0 |
Mitochondria | 2 | 0 | 1 | 4 | 8 | 13 | 19 | 18 | 31 | 27 | 39 | 53 | 0 | 1 | 0 | 2 | 2 | 0 | 0 | 1 | 0 | 1 | 1 | 1 |
Nucleus | 4 | 0 | 2 | 1 | 0 | 1 | 2 | 3 | 4 | 6 | 2 | 5 | 0 | 1 | 0 | 9 | 10 | 8 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 7 | 2 | 3 | 6 | 0 | 19 | 3 | 3 | 2 | 3 | 0 | 0 | 0 | 12 | 9 | 7 | 0 | 2 | 1 | 4 | 6 | 2 |
Unique Cell Count | 201 | 279 | 171 | 79 | 156 | 103 | 162 | 179 | 140 | 114 | 103 | 163 | 291 | 418 | 469 | 389 | 247 | 223 | 197 | 283 | 175 | 231 | 390 | 381 |
Labelled Cell Count | 233 | 316 | 209 | 94 | 176 | 126 | 183 | 202 | 169 | 136 | 128 | 200 | 319 | 466 | 497 | 444 | 294 | 251 | 197 | 283 | 175 | 231 | 390 | 381 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.3 | 12.5 | 9.4 | 8.1 | 8.9 | 7.8 | 7.4 | 8.6 | 6.8 | 6.3 | 6.3 | 6.7 | 10.1 | 11.4 | 12.1 | 13.7 | 13.9 | 12.7 | 9.2 | 9.4 | 10.4 |
Std Deviation (1e-4) | 1.6 | 2.5 | 1.7 | 1.2 | 1.4 | 1.9 | 1.2 | 1.7 | 1.2 | 1.2 | 1.3 | 1.3 | 1.5 | 1.9 | 1.8 | 2.6 | 3.0 | 2.8 | 1.5 | 1.5 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.21 | -0.07 | -0.28 | -0.35 | -0.12 | -0.47 | -0.58 | -0.58 | -0.49 | 0.1 | 0.28 | 0.37 | 0.54 | 0.56 | 0.43 | -0.03 | 0.01 | 0.14 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.7 | -2.4 | -1.4 | -4.3 | -4.2 | -3.0 | -3.3 | -2.8 | -3.3 | -4.0 | -3.8 | -5.9 | -2.6 | -1.6 | -3.1 |
Cytoplasm | 0.5 | -0.2 | -2.4 | -1.0 | -3.3 | -4.3 | -4.9 | -6.0 | -5.3 | 2.4 | 1.8 | 4.6 | -1.4 | -1.5 | -1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.4 | 4.3 | 3.9 | 6.2 | 6.4 | 8.5 | 7.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.6 | -0.2 | -0.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.422 | 9.6902 | 8.3505 | 7.8996 | 6.5466 | 8.5972 | 8.0651 | 10.5827 | 8.8661 | 6.9507 | 7.9736 | 9.294 | 7.0301 | 8.2682 | 6.9258 | 8.4368 | 5.6769 | 7.6133 |
Actin | 0.0306 | 0.0007 | 0.0145 | 0.0026 | 0.0188 | 0.0086 | 0.0322 | 0.0019 | 0.0168 | 0.0709 | 0.0044 | 0.0028 | 0.0015 | 0.0021 | 0.0157 | 0.004 | 0.0267 | 0.0012 |
Bud | 0.0007 | 0.0001 | 0.0033 | 0.0009 | 0.0008 | 0.0005 | 0.0013 | 0.0037 | 0.0008 | 0.0035 | 0.0006 | 0.0003 | 0.0001 | 0.0002 | 0.0005 | 0.0004 | 0.0011 | 0.0001 |
Bud Neck | 0.0023 | 0.0004 | 0.0046 | 0.0021 | 0.0014 | 0.0033 | 0.0038 | 0.0004 | 0.0012 | 0.0016 | 0.002 | 0.0027 | 0.0003 | 0.0009 | 0.0015 | 0.0011 | 0.0016 | 0.002 |
Bud Periphery | 0.0013 | 0.0001 | 0.0044 | 0.0021 | 0.0016 | 0.001 | 0.0018 | 0.0014 | 0.001 | 0.0052 | 0.0009 | 0.0009 | 0.0002 | 0.0002 | 0.0008 | 0.0004 | 0.0013 | 0.0002 |
Bud Site | 0.0115 | 0.0021 | 0.008 | 0.0026 | 0.0024 | 0.0013 | 0.0085 | 0.0173 | 0.0016 | 0.0032 | 0.0007 | 0.0002 | 0.0006 | 0.004 | 0.0051 | 0.0006 | 0.0014 | 0.0001 |
Cell Periphery | 0.0067 | 0.0043 | 0.0085 | 0.0044 | 0.0059 | 0.0031 | 0.0044 | 0.0029 | 0.0032 | 0.0016 | 0.002 | 0.0018 | 0.004 | 0.0023 | 0.0029 | 0.0015 | 0.0008 | 0.0013 |
Cytoplasm | 0.7617 | 0.9802 | 0.9078 | 0.8776 | 0.7708 | 0.8383 | 0.7976 | 0.962 | 0.9008 | 0.619 | 0.8839 | 0.9421 | 0.9449 | 0.9496 | 0.9094 | 0.9277 | 0.8668 | 0.9759 |
Cytoplasmic Foci | 0.0246 | 0.0008 | 0.0075 | 0.0046 | 0.0325 | 0.0065 | 0.0249 | 0.0023 | 0.0048 | 0.0183 | 0.0107 | 0.0046 | 0.0032 | 0.0035 | 0.0081 | 0.0075 | 0.0108 | 0.0023 |
Eisosomes | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0366 | 0.008 | 0.0089 | 0.0065 | 0.0066 | 0.0231 | 0.0188 | 0.0027 | 0.0081 | 0.0064 | 0.0435 | 0.0128 | 0.0235 | 0.0249 | 0.0128 | 0.0036 | 0.0142 | 0.0102 |
Endosome | 0.0239 | 0.0003 | 0.0063 | 0.0164 | 0.0337 | 0.0162 | 0.0297 | 0.0008 | 0.0203 | 0.0857 | 0.0273 | 0.0098 | 0.0057 | 0.0026 | 0.0108 | 0.0126 | 0.031 | 0.0028 |
Golgi | 0.0117 | 0.0002 | 0.0046 | 0.0081 | 0.0426 | 0.0104 | 0.0217 | 0.0003 | 0.0092 | 0.0197 | 0.0123 | 0.0043 | 0.0018 | 0.0009 | 0.0046 | 0.0022 | 0.0138 | 0.0008 |
Lipid Particles | 0.0191 | 0.0001 | 0.0054 | 0.0056 | 0.0196 | 0.0035 | 0.0103 | 0.0003 | 0.0041 | 0.0035 | 0.0011 | 0.0035 | 0.001 | 0.0009 | 0.0035 | 0.0022 | 0.0029 | 0.0003 |
Mitochondria | 0.0087 | 0 | 0.0012 | 0.0539 | 0.0204 | 0.0424 | 0.0134 | 0.0001 | 0.0147 | 0.0727 | 0.0042 | 0.0049 | 0.0073 | 0.0002 | 0.0108 | 0.0003 | 0.0047 | 0.0002 |
None | 0.0127 | 0.0011 | 0.0015 | 0.0007 | 0.0204 | 0.0217 | 0.0091 | 0.0008 | 0.0007 | 0.0036 | 0.0009 | 0.0005 | 0.0017 | 0.0034 | 0.0031 | 0.002 | 0.0045 | 0.0006 |
Nuclear Periphery | 0.0092 | 0.0002 | 0.0004 | 0.0005 | 0.0012 | 0.0112 | 0.0017 | 0.0002 | 0.0024 | 0.0065 | 0.0007 | 0.0008 | 0.0005 | 0.0003 | 0.0017 | 0.0079 | 0.004 | 0.0003 |
Nucleolus | 0.0011 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 |
Nucleus | 0.0035 | 0.0002 | 0.0008 | 0.0007 | 0.0007 | 0.0021 | 0.0013 | 0.0006 | 0.001 | 0.0026 | 0.0005 | 0.0005 | 0.0003 | 0.0004 | 0.0009 | 0.0022 | 0.0015 | 0.0003 |
Peroxisomes | 0.0115 | 0 | 0.0063 | 0.0008 | 0.0161 | 0.0008 | 0.0095 | 0 | 0.0009 | 0.0107 | 0.0002 | 0.0052 | 0.0002 | 0 | 0.0015 | 0.0005 | 0.0068 | 0 |
Punctate Nuclear | 0.0088 | 0 | 0.0002 | 0.0001 | 0.0011 | 0.0015 | 0.0009 | 0.0002 | 0.0004 | 0.0061 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0013 | 0.0196 | 0.0009 | 0 |
Vacuole | 0.0117 | 0.0012 | 0.0054 | 0.0085 | 0.0024 | 0.0022 | 0.0072 | 0.0017 | 0.0058 | 0.0393 | 0.0032 | 0.0013 | 0.003 | 0.0032 | 0.0039 | 0.003 | 0.0038 | 0.0011 |
Vacuole Periphery | 0.0014 | 0 | 0.0002 | 0.0012 | 0.0007 | 0.0017 | 0.0013 | 0.0001 | 0.0017 | 0.0194 | 0.0006 | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.0653 | 20.4742 | 21.1507 | 37.3003 | 26.4928 | 25.5837 | 33.4877 | 45.0669 | 41.0428 | 35.2405 |
Translational Efficiency | 0.9114 | 0.9343 | 1.0371 | 0.7699 | 0.8533 | 0.9604 | 0.7544 | 0.7595 | 0.9352 | 0.7399 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1796 | 1273 | 100 | 244 | 1667 | 2104 | 157 | 683 | 3463 | 3377 | 257 | 927 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 950.30 | 1160.37 | 1459.76 | 1489.68 | 926.38 | 1046.57 | 1385.84 | 1349.13 | 938.79 | 1089.47 | 1414.60 | 1386.12 |
Standard Deviation | 130.16 | 190.07 | 180.08 | 188.93 | 156.75 | 152.96 | 178.68 | 184.01 | 144.07 | 176.74 | 182.81 | 195.38 |
Intensity Change Log 2 | — | 0.288130 | 0.619276 | 0.648548 | — | 0.175993 | 0.581085 | 0.542353 | — | 0.233865 | 0.600550 | 0.597104 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001162 | 0.001057 | 0.005367 | 0.003761 | 0.000486 | 0.002321 | 0.002751 | 0.001709 | 0.000837 | 0.001844 | 0.003769 | 0.002249 |
Bud Neck | 0.003761 | 0.001396 | 0.006346 | 0.004361 | 0.002370 | 0.005452 | 0.004964 | 0.002997 | 0.003091 | 0.003923 | 0.005502 | 0.003356 |
Bud Site | 0.039561 | 0.035350 | 0.065024 | 0.062525 | 0.027737 | 0.047135 | 0.051671 | 0.039130 | 0.033869 | 0.042692 | 0.056867 | 0.045288 |
Cell Periphery | 0.001547 | 0.000754 | 0.002495 | 0.004428 | 0.000572 | 0.000766 | 0.000961 | 0.001163 | 0.001078 | 0.000761 | 0.001558 | 0.002022 |
Cytoplasm | 0.827011* | 0.832317* | 0.665775* | 0.789906* | 0.812668* | 0.759962* | 0.476688* | 0.787804* | 0.820107* | 0.787237* | 0.550262* | 0.788357* |
Cytoplasmic Foci | 0.076373 | 0.080687 | 0.104513 | 0.032980 | 0.089980 | 0.118426 | 0.141305 | 0.043662 | 0.082923 | 0.104199 | 0.126989 | 0.040850 |
Eisosomes | 0.000202 | 0.000034 | 0.000052 | 0.000055 | 0.000068 | 0.000164 | 0.000085 | 0.000032 | 0.000138 | 0.000115 | 0.000072 | 0.000038 |
Endoplasmic Reticulum | 0.002783 | 0.003355 | 0.004137 | 0.000210 | 0.001921 | 0.001075 | 0.000727 | 0.000223 | 0.002368 | 0.001934 | 0.002054 | 0.000219 |
Endosome | 0.004247 | 0.004490 | 0.006775 | 0.001278 | 0.006907 | 0.006334 | 0.012534 | 0.004558 | 0.005527 | 0.005639 | 0.010293 | 0.003695 |
Golgi | 0.007597 | 0.006985 | 0.002550 | 0.000700 | 0.006397 | 0.012193 | 0.001302 | 0.004541 | 0.007019 | 0.010230 | 0.001788 | 0.003530 |
Lipid Particles | 0.001300 | 0.001639 | 0.014031 | 0.011093 | 0.001276 | 0.001356 | 0.004963 | 0.005090 | 0.001288 | 0.001463 | 0.008492 | 0.006670 |
Mitochondria | 0.001606 | 0.000500 | 0.000009 | 0.000003 | 0.001001 | 0.001954 | 0.000174 | 0.000118 | 0.001315 | 0.001406 | 0.000109 | 0.000088 |
Mitotic Spindle | 0.000196 | 0.003439 | 0.001705 | 0.000124 | 0.000361 | 0.001833 | 0.002406 | 0.001107 | 0.000276 | 0.002438 | 0.002133 | 0.000848 |
None | 0.001298 | 0.001073 | 0.000235 | 0.000212 | 0.001775 | 0.001215 | 0.000115 | 0.000189 | 0.001528 | 0.001161 | 0.000162 | 0.000195 |
Nuclear Periphery | 0.000012 | 0.000048 | 0.000072 | 0.000020 | 0.000066 | 0.000015 | 0.000235 | 0.000063 | 0.000038 | 0.000028 | 0.000172 | 0.000051 |
Nuclear Periphery Foci | 0.000531 | 0.001201 | 0.016800 | 0.005953 | 0.000717 | 0.000433 | 0.008195 | 0.002698 | 0.000620 | 0.000722 | 0.011543 | 0.003554 |
Nucleolus | 0.000090 | 0.000209 | 0.000031 | 0.000037 | 0.000131 | 0.000187 | 0.002786 | 0.000096 | 0.000110 | 0.000195 | 0.001714 | 0.000080 |
Nucleus | 0.004727 | 0.003463 | 0.009536 | 0.002723 | 0.006580 | 0.005828 | 0.005665 | 0.002990 | 0.005619 | 0.004936 | 0.007171 | 0.002920 |
Peroxisomes | 0.000968 | 0.001068 | 0.000828 | 0.001101 | 0.000857 | 0.002045 | 0.005853 | 0.001408 | 0.000915 | 0.001677 | 0.003898 | 0.001327 |
Vacuole | 0.023221 | 0.019213 | 0.092644 | 0.078400 | 0.036313 | 0.029428 | 0.276100* | 0.098860 | 0.029523 | 0.025577 | 0.204717* | 0.093475 |
Vacuole Periphery | 0.001808 | 0.001724 | 0.001076 | 0.000132 | 0.001814 | 0.001880 | 0.000519 | 0.001564 | 0.001811 | 0.001821 | 0.000736 | 0.001187 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.26 | -4.32 | -4.20 | -5.17 | 1.76 | -3.35 | -3.13 | -5.40 | 1.27 | 1.46 | -2.59 | -4.93 | -4.84 | -0.70 | 2.73 |
Bud Neck | 10.01 | -3.71 | -1.33 | -7.78 | 2.86 | -6.78 | -4.50 | -2.37 | 5.61 | 3.50 | -2.77 | -5.38 | -1.04 | 2.01 | 4.89 |
Bud Site | 2.08 | -4.43 | -5.78 | -6.66 | 0.87 | -9.27 | -5.52 | -4.13 | 3.46 | 3.11 | -5.87 | -6.63 | -5.02 | -0.70 | 3.59 |
Cell Periphery | 7.13 | -2.74 | -4.57 | -6.09 | -2.52 | -3.94 | -2.08 | -4.90 | -2.78 | -0.79 | 5.45 | -2.48 | -4.36 | -6.43 | -1.37 |
Cytoplasm | -0.79 | 7.54 | 4.63 | 4.85 | -5.08 | 8.29 | 16.63 | 5.33 | -0.36 | -13.52 | 7.31 | 17.09 | 7.47 | 2.80 | -13.05 |
Cytoplasmic Foci | -1.15 | -3.81 | 10.33 | 10.22 | 7.97 | -7.15 | -5.42 | 12.34 | 19.81 | 10.55 | -7.67 | -6.88 | 15.27 | 21.38 | 12.93 |
Eisosomes | 3.71 | 3.23 | 3.24 | -2.41 | 0.08 | -7.99 | -1.17 | 5.10 | 13.24 | 6.35 | 0.92 | 2.78 | 4.24 | 11.48 | 5.64 |
Endoplasmic Reticulum | -0.53 | -0.37 | 5.20 | 3.31 | 1.89 | 1.65 | 2.67 | 3.72 | 3.93 | 4.43 | 0.85 | 0.83 | 6.36 | 4.50 | 2.29 |
Endosome | -0.34 | -1.56 | 7.18 | 5.10 | 3.71 | 0.80 | -2.93 | 2.73 | 2.37 | 4.47 | -0.22 | -3.66 | 3.06 | 3.23 | 5.14 |
Golgi | 0.76 | 11.33 | 17.55 | 8.92 | 6.25 | -7.26 | 9.32 | 1.69 | 5.10 | -1.62 | -5.60 | 14.45 | 3.53 | 5.96 | -1.12 |
Lipid Particles | -1.89 | -6.04 | -7.15 | -6.84 | 1.52 | -0.64 | -6.26 | -7.53 | -7.46 | -0.20 | -1.76 | -7.62 | -10.29 | -9.91 | 1.96 |
Mitochondria | 1.80 | 2.90 | 2.91 | 1.88 | 3.66 | -1.32 | 1.72 | 1.86 | 3.45 | 0.30 | -0.18 | 3.26 | 3.36 | 3.81 | 0.19 |
Mitotic Spindle | -2.40 | -2.62 | 0.69 | 2.44 | 2.73 | -2.77 | -1.31 | -1.22 | 1.37 | 0.82 | -3.56 | -2.05 | -1.33 | 2.54 | 1.40 |
None | 1.12 | 6.69 | 6.50 | 5.24 | 0.44 | 2.20 | 6.97 | 6.69 | 9.17 | -0.87 | 2.31 | 9.69 | 9.45 | 10.42 | -0.33 |
Nuclear Periphery | -1.59 | -6.99 | -6.33 | 1.14 | 5.99 | 1.59 | -0.93 | 0.12 | -1.33 | 0.96 | 0.56 | -1.32 | -0.43 | -0.85 | 1.16 |
Nuclear Periphery Foci | -2.74 | -8.92 | -9.17 | -7.47 | 5.82 | 4.33 | -7.11 | -7.10 | -8.21 | 4.97 | -1.05 | -11.00 | -11.21 | -10.33 | 7.80 |
Nucleolus | -0.94 | 10.37 | 10.54 | 1.21 | -1.00 | -1.20 | -0.96 | 3.43 | 1.99 | 0.98 | -1.41 | -0.95 | 4.41 | 1.80 | 0.98 |
Nucleus | 4.18 | -4.19 | 5.77 | 1.83 | 5.41 | 1.37 | 0.68 | 5.21 | 3.19 | 1.28 | 1.90 | -1.99 | 5.53 | 3.12 | 3.85 |
Peroxisomes | -0.63 | 0.84 | -1.10 | 0.23 | -1.74 | -4.86 | -1.28 | -3.12 | 3.05 | 1.12 | -4.22 | -1.24 | -3.49 | 2.31 | 1.05 |
Vacuole | 2.77 | -5.90 | -9.76 | -10.36 | 1.02 | 3.73 | -12.52 | -14.68 | -16.31 | 7.77 | 3.40 | -12.95 | -18.14 | -19.21 | 6.62 |
Vacuole Periphery | 0.20 | 1.59 | 6.56 | 4.68 | 1.47 | -0.15 | 5.49 | 0.72 | 0.72 | -1.09 | -0.03 | 4.28 | 1.49 | 1.38 | -0.60 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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