Standard name
Human Ortholog
Description Basic helix-loop-helix (bHLH) protein; forms homodimer to bind E-box consensus sequence CACGTG present at MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins, required for chromosome segregation; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.63 0.65 0.5 0.48 0.44 0.36 0.33 0.3 0.28 0.25 0.2 0.28 0.41 0.53 0.48 0.53 0.6 0.64 0.63 0.62 0.49 0.32 0.27 0.49
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.06 0.05 0.13 0.09 0.14 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.34 0.33 0.42 0.43 0.44 0.42 0.38 0.37 0.38 0.37 0.41 0.35 0.56 0.43 0.42 0.31 0.3 0.24 0.31 0.31 0.44 0.37 0.38 0.21
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0.05 0.08 0 0 0.13 0.18 0.16 0.19 0.21 0.29 0.22 0 0 0.06 0 0 0.07 0 0 0 0.2 0.16 0.15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.06 0.12 0.13 0.15 0.16 0.2 0.16 0.21 0.19 0.22 0.06 0 0 0.15 0.09 0.09 0 0 0 0.07 0.1 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 1 3 4 7 3 11 5 0 0 0 0 0 0 4 1 0 1 5 6
Bud Neck 0 0 0 0 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 2 0 2 6 5 0 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 1 2 3 4 1 2 4 2 0 0 0 7 2 2 0 0 0 0 1 1
Cytoplasm 120 130 85 115 124 104 121 132 66 82 68 79 66 106 94 145 96 129 124 135 86 45 71 90
Endoplasmic Reticulum 0 0 0 0 0 0 2 1 1 0 2 3 0 0 0 5 0 4 0 1 1 0 2 1
Endosome 1 0 0 1 0 1 0 0 2 2 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4 1 1 0 1 0 0 0 0
Mitochondria 0 0 1 5 1 17 24 23 31 28 48 20 0 0 0 2 2 4 0 0 0 0 1 2
Nucleus 65 67 72 103 126 120 141 165 90 120 137 98 90 87 82 86 48 49 60 67 77 52 100 38
Nuclear Periphery 0 0 0 0 1 0 0 0 0 1 2 1 0 0 0 0 0 0 0 0 0 1 1 0
Nucleolus 11 10 13 9 10 37 65 72 46 68 98 60 5 7 12 10 7 14 4 3 4 28 42 27
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 3 1 0 0 2 0 1 0 6 1
Vac/Vac Membrane 2 5 11 29 36 44 59 90 38 69 63 61 9 8 6 41 15 18 0 3 3 10 25 11
Unique Cell Count 191 201 171 241 285 286 371 444 238 324 338 279 162 200 194 274 161 202 198 217 177 142 264 184
Labelled Cell Count 199 212 183 262 300 326 422 492 284 382 438 332 171 208 199 301 171 222 198 217 177 142 264 184


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.1 12.3 11.1 11.0 12.2 11.4 11.4 12.2 11.0 11.6 11.3 12.9 15.0 16.1 17.0 23.9 23.0 21.8 17.8 16.7 16.7
Std Deviation (1e-4) 4.6 5.9 4.2 4.7 4.3 3.9 4.0 3.8 3.6 4.1 4.0 4.2 6.6 5.9 6.8 8.6 7.8 7.4 8.7 7.8 8.2
Intensity Change (Log2) – – – -0.01 0.15 0.04 0.04 0.14 -0.01 0.07 0.02 0.22 0.44 0.54 0.62 1.11 1.05 0.98 0.68 0.6 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 -1.3 -2.8 -3.8 -4.6 -4.5 -5.5 -6.9 -4.6 -1.6 0.6 -0.2 0.7 1.8 2.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.9 3.0 2.6 4.6 3.6 4.9 3.2 0 0 0 0 0 0
Nucleus 0.1 0.4 0 -0.9 -1.1 -0.9 -1.1 -0.3 -1.5 2.5 0.3 0 -2.3 -2.3 -3.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.7 -1.9 1.8 3.1 2.8 3.3 3.8 5.5 3.9 -1.8 -1.7 -0.5 -1.8 -1.2 -0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.9 2.1 2.8 3.1 4.1 2.9 4.3 3.7 4.3 -0.3 -1.1 -1.5 2.7 1.0 0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.7206 10.8584 10.0135 9.7113 8.3877 10.2071 11.8167 10.6095 10.0916 9.6849 8.8113 9.6025 12.8194 10.8602 10.5845 9.6963 9.2304 10.919
Actin 0.0174 0 0.0249 0.0016 0.0149 0.0023 0.0535 0 0.0087 0.004 0.001 0.0041 0.0184 0 0.0004 0.0021 0.0086 0
Bud 0.0008 0 0.0008 0.0001 0.0025 0.0002 0.0005 0 0 0.0004 0.0001 0.0001 0.0002 0 0.0001 0.0003 0.0001 0
Bud Neck 0.0007 0.0001 0.0005 0.0001 0.0014 0.0007 0.0023 0 0.0003 0.0001 0.0002 0.0004 0.0006 0 0.0001 0.0002 0.0003 0.0002
Bud Periphery 0.0015 0.0001 0.0013 0.0003 0.0022 0.0004 0.0007 0 0 0.0005 0.0002 0.0002 0.0004 0 0.0003 0.0006 0.0004 0.0001
Bud Site 0.0026 0 0.0025 0.0003 0.0246 0.0002 0.0033 0 0.0002 0.0005 0.0002 0.0001 0.0021 0 0.0003 0.0007 0.0002 0
Cell Periphery 0.0008 0 0.0006 0.0002 0.0007 0.0003 0.0004 0 0 0 0.0001 0.0001 0.0001 0 0.0001 0.0006 0.0002 0
Cytoplasm 0.008 0 0.004 0.001 0.0049 0.0023 0.0028 0 0.0013 0.0006 0.0072 0.0003 0.0007 0 0.0015 0.0003 0.0003 0
Cytoplasmic Foci 0.0263 0 0.0203 0.0077 0.0389 0.0063 0.0053 0 0.0082 0.0099 0.0154 0.0022 0.0039 0 0.002 0.0099 0.0032 0
Eisosomes 0.0001 0 0.0002 0.0001 0.0002 0 0.0005 0 0.0001 0 0 0 0.0008 0 0 0 0.0003 0
Endoplasmic Reticulum 0.0024 0 0.0012 0.0021 0.0016 0.001 0.0028 0 0.0009 0.0005 0.0011 0.0013 0.0008 0 0.0008 0.0003 0.0003 0
Endosome 0.0206 0.0001 0.0127 0.0157 0.0295 0.0128 0.0084 0 0.0039 0.0129 0.0164 0.0071 0.0041 0 0.003 0.0032 0.0041 0
Golgi 0.0071 0 0.0086 0.003 0.0174 0.0037 0.0048 0 0.0027 0.0057 0.0063 0.0019 0.0033 0 0.0004 0.003 0.0047 0
Lipid Particles 0.009 0 0.0184 0.0097 0.0877 0.0087 0.0058 0 0.0018 0.0041 0.0426 0.0051 0.0021 0 0.002 0.0059 0.0482 0
Mitochondria 0.0118 0.0002 0.0239 0.0153 0.0443 0.0029 0.0037 0.0001 0.0004 0.0011 0.0106 0.013 0.0021 0.0002 0.0006 0.0043 0.0043 0.0002
None 0.0064 0 0.0022 0.0017 0.0021 0.0007 0.0019 0 0.0049 0.0003 0.0017 0.0006 0.0144 0 0.0008 0.0004 0.0022 0
Nuclear Periphery 0.008 0.001 0.005 0.0041 0.0079 0.0022 0.0044 0.0004 0.0066 0.0028 0.0092 0.0149 0.003 0.0003 0.0044 0.0026 0.019 0.0002
Nucleolus 0.0491 0.0171 0.025 0.029 0.1972 0.1428 0.0205 0.0157 0.0178 0.0083 0.1512 0.0892 0.016 0.0215 0.0157 0.0444 0.3089 0.0602
Nucleus 0.7836 0.9806 0.8278 0.8935 0.4635 0.8025 0.87 0.9835 0.9353 0.9366 0.6947 0.8444 0.9227 0.9779 0.9637 0.8985 0.5609 0.9387
Peroxisomes 0.0053 0 0.0065 0.0037 0.0245 0.0027 0.001 0 0.0013 0.0031 0.0131 0.0081 0.002 0 0.0003 0.0162 0.0136 0
Punctate Nuclear 0.0252 0.0002 0.0053 0.0019 0.0213 0.0042 0.0057 0.0001 0.0053 0.0079 0.0275 0.0054 0.0015 0.0001 0.0026 0.0008 0.0179 0.0001
Vacuole 0.0059 0.0004 0.004 0.0032 0.006 0.0017 0.0009 0 0.0001 0.0003 0.0006 0.0004 0.0003 0 0.0005 0.003 0.0006 0.0001
Vacuole Periphery 0.0075 0.0002 0.0042 0.0059 0.0066 0.0014 0.0008 0 0.0002 0.0003 0.0006 0.0009 0.0004 0 0.0003 0.0028 0.0018 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.8692 39.2309 34.656 35.1089 44.7066 38.6395 36.833 41.1143 46.2499 43.8858
Translational Efficiency 3.2708 2.919 2.6633 3.1889 2.8688 2.9086 2.5291 2.8201 2.4145 2.5757

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Basic helix-loop-helix (bHLH) protein; forms homodimer to bind E-box consensus sequence CACGTG present at MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins, required for chromosome segregation; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Cbf1

Cbf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cbf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available