Standard name
Human Ortholog
Description Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0.08 0.12 0.08 0.06 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0.09 0.21 0.14 0.33 0.18 0.38 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.93 0.98 0.96 0.94 0.9 0.91 0.88 0.86 0.83 0.85 0.84 0.86 0.95 0.9 0.86 0.84 0.86 0.89 0.94 0.96 0.9 0.82 0.71 0.64
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0.07 0.09 0.19 0.12 0.19 0.14 0 0 0 0 0 0 0 0 0 0.06 0.08 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.05 0 0 0 0.09 0.14 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 1 3 4 0 2 1 0 0 0 0 0 0 2 0 1 1 4 4
Bud Neck 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 2 3 6 7 11 7 12 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0
Cytoplasm 9 0 6 9 25 10 11 15 4 7 4 9 8 14 27 20 17 6 0 0 1 0 1 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 3 2 0 0 0 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 1 1 0 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 0 0 0 0 0 0
Mitochondria 13 1 1 3 4 28 75 53 85 50 96 48 1 1 0 1 3 6 1 0 2 1 2 4
Nucleus 267 249 214 165 329 287 308 326 212 238 214 234 169 160 191 220 249 249 271 247 207 188 201 211
Nuclear Periphery 1 0 1 0 1 4 0 2 3 2 3 1 0 4 2 0 1 6 0 1 0 0 4 2
Nucleolus 8 3 0 3 6 15 25 33 49 35 49 37 1 1 3 3 5 5 1 1 3 14 23 34
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 1 1 1 0 0 0 0 0 0 1 1 1 0 0 0 1 0 2
Vac/Vac Membrane 5 3 3 1 6 3 2 2 2 8 0 0 0 0 3 21 16 15 7 4 9 20 39 61
Unique Cell Count 287 255 224 176 366 314 349 377 254 281 256 271 178 177 222 263 290 280 290 259 231 230 283 330
Labelled Cell Count 303 256 226 181 374 350 429 442 372 348 380 339 179 182 227 272 298 292 290 259 231 230 283 330


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 9.5 7.8 7.4 7.4 6.1 5.4 6.0 5.2 5.7 4.9 5.4 7.8 8.5 8.4 13.5 13.4 12.3 8.1 7.8 8.9
Std Deviation (1e-4) 1.8 1.7 1.4 1.6 1.7 1.3 1.2 1.2 1.2 1.3 1.2 1.3 1.6 2.0 2.0 3.9 3.7 3.2 1.6 1.5 1.6
Intensity Change (Log2) – – – -0.08 -0.08 -0.37 -0.53 -0.38 -0.6 -0.46 -0.69 -0.52 -0.0 0.11 0.11 0.79 0.78 0.65 0.05 -0.0 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.2 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 2.2 0.3 0.3 0.8 0 -0.1 0 0.4 1.0 2.4 3.8 2.4 1.7 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.3 7.2 5.6 9.4 6.4 0 6.4 0 0 0 0 0 0
Nucleus -0.8 -2.5 -1.9 -3.0 -3.6 -4.2 -3.9 -4.2 -3.5 -0.3 -2.0 -3.5 -4.2 -3.6 -2.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.3 4.1 4.6 6.9 5.5 0 5.7 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 3.4 2.5 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0792 3.5178 3.0604 1.9749 1.8562 3.2749 5.3027 5.6405 5.4543 5.6646 4.8796 5.1366 4.488 4.7903 4.7633 4.3033 3.8272 4.5415
Actin 0.0059 0.0002 0.0071 0.001 0.0201 0.0006 0.0064 0 0.0056 0 0.0014 0.0033 0.0513 0.0002 0.0046 0 0.0233 0.0001
Bud 0.0046 0.0056 0.0007 0.0032 0.0002 0.0038 0.0002 0 0.0001 0 0.0001 0 0.0006 0.0001 0.0001 0 0.0015 0
Bud Neck 0.0012 0.0003 0.0007 0.0006 0.0002 0.0018 0.0007 0 0.0003 0 0.0002 0.0005 0.0005 0.0001 0 0 0.0003 0.0006
Bud Periphery 0.0049 0.0025 0.001 0.0039 0.0002 0.0064 0.0007 0 0.0002 0 0.0001 0 0.0009 0 0 0 0.0043 0.0001
Bud Site 0.0079 0.0136 0.0017 0.0014 0.0003 0.0002 0.0008 0 0.0034 0 0.0002 0 0.0016 0.0006 0.0003 0 0.0009 0.0001
Cell Periphery 0.0009 0.0005 0.0002 0.0004 0 0.0003 0.0002 0 0.0001 0 0.0001 0 0.001 0.0002 0 0 0.0003 0.0002
Cytoplasm 0.0393 0.026 0.0068 0.0834 0.017 0.0262 0.0015 0 0.0003 0 0.0021 0 0.0278 0.024 0.0009 0 0.0384 0.0222
Cytoplasmic Foci 0.0288 0.0014 0.0125 0.0096 0.0207 0.0081 0.0036 0 0.0007 0 0.0067 0.0001 0.0191 0.0002 0.0033 0 0.053 0.0003
Eisosomes 0.0001 0.0002 0.0003 0.0001 0.0001 0 0.0001 0 0 0 0 0 0.0004 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0113 0.0006 0.0011 0.0046 0.0022 0.0007 0.0014 0 0.0002 0 0.0017 0 0.0085 0.001 0.0007 0 0.0058 0.0014
Endosome 0.0314 0.0028 0.0165 0.0069 0.0255 0.0131 0.0043 0 0.0009 0 0.0093 0.0002 0.0065 0.0002 0.0044 0 0.01 0.0136
Golgi 0.0116 0.0004 0.0053 0.0017 0.0097 0.0054 0.0014 0 0.0005 0 0.0007 0.0002 0.0041 0 0.007 0 0.0023 0.0023
Lipid Particles 0.0334 0.0008 0.0051 0.0443 0.0569 0.0054 0.0076 0 0.0004 0 0.0088 0.0001 0.0037 0 0.0013 0 0.002 0.0013
Mitochondria 0.0075 0.002 0.0105 0.0216 0.01 0.0164 0.003 0 0.0007 0 0.0014 0.0001 0.0088 0.0002 0.0025 0.0001 0.0712 0.0019
None 0.182 0.2368 0.0413 0.2529 0.4845 0.2131 0.0012 0 0.0002 0 0.0055 0 0.0052 0.0004 0.0003 0 0.0232 0
Nuclear Periphery 0.026 0.0031 0.0077 0.0149 0.0291 0.0086 0.0077 0.0007 0.0017 0.0003 0.0192 0.0006 0.0098 0.0014 0.0008 0.0002 0.0468 0.0008
Nucleolus 0.0135 0.0085 0.0132 0.0051 0.0046 0.0365 0.0109 0.0092 0.0078 0.0032 0.06 0.0251 0.0158 0.0094 0.0066 0.0023 0.0092 0.0338
Nucleus 0.5468 0.6791 0.8276 0.5183 0.1998 0.6131 0.9312 0.9898 0.9744 0.9963 0.8474 0.9692 0.8054 0.9603 0.9627 0.9973 0.6869 0.9189
Peroxisomes 0.0083 0.0001 0.0047 0.0091 0.1087 0.0014 0.0064 0 0.0014 0 0.0008 0.0001 0.0061 0 0.0036 0 0.0049 0
Punctate Nuclear 0.0192 0.0077 0.0333 0.0093 0.0084 0.034 0.006 0.0002 0.0006 0.0001 0.0301 0.0002 0.0088 0.0003 0.0006 0 0.0047 0.0001
Vacuole 0.0128 0.0046 0.0019 0.005 0.0005 0.003 0.0025 0 0.0003 0 0.0024 0 0.0132 0.0007 0.0001 0 0.0031 0.0015
Vacuole Periphery 0.0027 0.0032 0.0008 0.0027 0.0012 0.0021 0.0021 0 0.0001 0 0.0019 0 0.0009 0.0007 0.0001 0 0.0076 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 48.3129 36.2229 33.0817 37.4775 33.0038 45.6901 38.5031 39.9312 36.2285 43.9692
Translational Efficiency 1.3068 1.4609 1.3351 1.1998 1.4203 1.3245 1.214 0.9128 1.1328 1.2222

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1909 222 2071 2117 1440 1173 118 1400 3349 1395 2189 3517

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 939.06 1089.87 1099.35 1385.41 823.84 985.60 1324.09 1336.39 889.52 1002.19 1111.46 1365.90
Standard Deviation 140.22 160.50 240.20 250.99 128.50 156.25 186.43 255.74 146.84 161.50 242.97 254.03
Intensity Change Log 2 — 0.214867 0.227362 0.561024 — 0.258638 0.684565 0.697905 — 0.235487 0.459106 0.626610

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000306 0.000387 0.000385 0.000585 0.000270 0.000591 0.000581 0.000692 0.000290 0.000559 0.000395 0.000628
Bud Neck 0.004057 0.004811 0.008842 0.019919 0.002935 0.008468 0.006273 0.021519 0.003575 0.007886 0.008703 0.020556
Bud Site 0.000692 0.000365 0.000433 0.002465 0.000317 0.001449 0.000382 0.002116 0.000531 0.001276 0.000430 0.002326
Cell Periphery 0.000159 0.000040 0.000342 0.000141 0.000256 0.000477 0.000018 0.000181 0.000201 0.000408 0.000325 0.000157
Cytoplasm 0.000264 0.000085 0.000968 0.001425 0.000698 0.000287 0.000027 0.001545 0.000451 0.000255 0.000917 0.001473
Cytoplasmic Foci 0.000121 0.000013 0.000032 0.000584 0.000020 0.000117 0.000008 0.000862 0.000078 0.000101 0.000031 0.000695
Eisosomes 0.000036 0.000032 0.000061 0.000037 0.000043 0.000081 0.000067 0.000037 0.000039 0.000074 0.000062 0.000037
Endoplasmic Reticulum 0.008152 0.002845 0.002256 0.001917 0.007008 0.011061 0.002557 0.002320 0.007660 0.009754 0.002273 0.002078
Endosome 0.000236 0.000071 0.000056 0.001148 0.000146 0.000618 0.000026 0.001403 0.000198 0.000531 0.000054 0.001250
Golgi 0.000092 0.000037 0.000024 0.000854 0.000025 0.000098 0.000020 0.001207 0.000063 0.000089 0.000024 0.000995
Lipid Particles 0.000036 0.000018 0.000008 0.000059 0.000019 0.000075 0.000014 0.000158 0.000028 0.000066 0.000008 0.000098
Mitochondria 0.000647 0.000688 0.000768 0.003069 0.000420 0.001439 0.000890 0.003567 0.000549 0.001320 0.000775 0.003267
Mitotic Spindle 0.000699 0.001347 0.000109 0.006408 0.000099 0.003092 0.000004 0.008086 0.000441 0.002815 0.000104 0.007076
None 0.001139 0.000648 0.002786 0.001289 0.002469 0.001372 0.000278 0.001149 0.001711 0.001257 0.002651 0.001233
Nuclear Periphery 0.000274 0.000079 0.000119 0.000926 0.000172 0.000819 0.000064 0.001854 0.000230 0.000701 0.000116 0.001296
Nuclear Periphery Foci 0.000062 0.000044 0.000085 0.000893 0.000114 0.000259 0.000004 0.000700 0.000084 0.000225 0.000081 0.000816
Nucleolus 0.008757 0.012888 0.004879 0.005390 0.011177 0.011522 0.007137 0.007710 0.009798 0.011739 0.005000 0.006313
Nucleus 0.973161* 0.974627* 0.977182* 0.949507* 0.972863* 0.952545* 0.980749* 0.940081* 0.973033* 0.956059* 0.977374* 0.945755*
Peroxisomes 0.000256 0.000444 0.000149 0.000152 0.000139 0.000310 0.000385 0.000222 0.000205 0.000332 0.000161 0.000180
Vacuole 0.000661 0.000381 0.000447 0.001972 0.000610 0.003525 0.000461 0.003138 0.000639 0.003024 0.000447 0.002436
Vacuole Periphery 0.000193 0.000151 0.000069 0.001258 0.000201 0.001792 0.000055 0.001454 0.000197 0.001531 0.000068 0.001336

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.04 -2.13 -6.22 -3.28 -6.17 -2.61 -6.78 -7.70 -1.26 -2.30 -2.54 -4.21 -9.80 -1.19 -8.14
Bud Neck -0.83 -9.38 -11.87 -10.33 -8.74 -5.40 -3.83 -12.55 -7.85 -9.16 -4.84 -13.07 -17.01 -10.64 -12.11
Bud Site 1.45 1.04 -4.96 -6.60 -6.41 -3.69 -1.13 -6.28 -1.86 -6.05 -2.63 0.51 -7.37 -3.55 -8.40
Cell Periphery 4.59 -0.90 0.81 -4.12 1.01 -1.36 3.20 1.03 2.05 -4.07 -1.63 -0.62 1.30 2.08 0.90
Cytoplasm 1.95 -1.66 -3.00 -3.34 -1.52 0.93 2.30 -1.25 -1.74 -2.29 0.59 -1.12 -3.05 -3.30 -1.65
Cytoplasmic Foci 1.60 0.48 -2.17 -2.98 -2.42 -1.98 1.84 -2.32 -2.05 -2.35 -0.21 0.06 -3.20 -3.14 -3.10
Eisosomes 1.05 -5.15 -0.09 -1.16 5.25 -3.33 -3.65 1.06 3.99 5.11 -3.69 -4.83 0.82 4.00 5.66
Endoplasmic Reticulum 10.85 14.68 14.39 2.63 1.56 -4.92 9.36 9.20 11.86 0.63 -3.16 17.68 16.94 12.59 0.90
Endosome 3.12 2.49 -2.71 -3.37 -3.29 -3.69 3.52 -2.91 -1.52 -3.24 -3.00 2.40 -3.97 -2.28 -4.48
Golgi 1.18 1.28 -2.13 -2.43 -2.45 -2.50 0.64 -2.17 -2.00 -2.18 -0.35 1.16 -3.06 -3.01 -3.25
Lipid Particles 1.57 2.02 -1.50 -3.32 -3.77 -2.19 0.91 -2.32 -1.18 -2.40 -1.62 2.04 -2.77 -0.97 -3.56
Mitochondria -0.16 -1.00 -6.10 -5.50 -6.04 -3.44 -4.42 -7.86 -4.43 -6.61 -2.99 -2.71 -9.42 -5.45 -8.86
Mitotic Spindle -0.45 1.75 -4.39 -2.78 -5.05 -2.21 1.92 -4.16 -2.03 -4.22 -1.99 1.68 -6.08 -2.73 -6.53
None 1.55 -2.50 -0.59 -1.90 2.03 1.68 4.05 2.29 0.63 -7.05 1.09 -1.43 1.23 -0.02 2.19
Nuclear Periphery 3.40 2.92 -4.89 -6.59 -6.37 -1.71 2.97 -3.50 -1.60 -3.73 -1.47 3.35 -5.26 -1.55 -5.88
Nuclear Periphery Foci 0.70 -0.80 -1.96 -2.02 -1.61 -1.33 1.59 -2.52 -1.79 -3.26 -1.79 -0.60 -2.66 -2.07 -2.13
Nucleolus -1.48 4.37 1.66 2.05 -2.00 -0.35 2.35 0.74 1.06 -1.75 -1.67 5.96 1.79 2.90 -3.82
Nucleus -0.24 -1.74 7.55 5.29 8.77 5.28 -3.08 7.90 2.85 9.40 5.22 -2.28 10.84 3.63 12.10
Peroxisomes -0.70 0.72 0.98 1.23 0.21 -2.08 -4.43 -3.85 0.33 1.55 -1.15 0.14 -0.02 1.35 -0.20
Vacuole 2.21 1.91 -3.04 -3.53 -3.41 -4.24 1.20 -3.66 0.61 -3.87 -4.13 1.97 -4.65 0.62 -5.05
Vacuole Periphery 0.67 2.75 -2.76 -2.88 -3.15 -2.33 1.93 -2.58 0.65 -2.97 -2.33 3.11 -3.76 0.52 -4.29
Standard name
Human Ortholog
Description Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rfc2

Rfc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rfc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available