Standard name
Human Ortholog
Description Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.07 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.97 0.98 0.97 0.93 0.88 0.89 0.79 0.8 0.82 0.75 0.99 0.99 0.99 0.97 0.98 0.98 0.97 0.95 0.95 0.97 0.95 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.07 0.06 0.05 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0.05 0.09 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.09 0.05 0.07 0.12 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 7 1 9 8 4 6 0 0 0 0 0 1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2 1 2 1 0 1 0 0 0 0 0 2 0 4 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 4 1 4 4 10 5 22 11 4 9 1 6 7 1 8 4 0 0 0 0 0 0
Cytoplasm 451 282 184 186 229 256 316 228 238 195 137 137 392 656 738 255 333 273 431 258 174 206 363 420
Endoplasmic Reticulum 1 1 2 0 2 6 23 17 17 12 3 8 3 1 2 13 11 13 0 0 1 0 4 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Golgi 0 0 0 1 0 0 0 0 3 0 1 3 0 0 0 1 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 1 1 0 8 2 15 11 15 9 0 0 0 1 1 0 0 0 1 0 1 1
Nucleus 0 0 0 0 0 5 4 1 2 4 1 0 0 0 0 0 1 0 2 1 1 0 1 2
Nuclear Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 3 1 1 0 5 7 7 6 27 12 11 22 2 6 7 6 5 6 1 3 0 0 6 3
Unique Cell Count 454 283 189 189 237 274 361 256 302 243 168 182 395 663 746 263 339 279 444 272 183 213 384 441
Labelled Cell Count 456 285 191 189 242 279 377 261 335 254 176 195 398 669 754 278 360 300 444 272 183 213 384 441


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 41.7 50.5 33.2 31.2 24.6 19.7 17.9 18.5 16.6 16.9 15.0 16.4 36.8 36.9 36.0 71.2 78.3 74.6 45.5 43.2 44.8
Std Deviation (1e-4) 7.5 11.2 6.9 7.1 5.3 4.2 3.9 4.0 3.9 4.0 3.7 3.5 8.9 8.0 6.8 24.5 26.4 27.2 10.7 10.3 10.2
Intensity Change (Log2) – – – -0.09 -0.43 -0.75 -0.89 -0.84 -1.0 -0.97 -1.15 -1.02 0.15 0.15 0.12 1.1 1.24 1.17 0.46 0.38 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 2.5 1.4 0 1.5 0 0 0 0 0 0
Cytoplasm 0.7 -0.4 -1.9 -3.8 -3.3 -5.8 -5.4 -5.0 -6.2 1.8 1.6 1.6 -0.2 0.7 0.3
Endoplasmic Reticulum 0 0 0 2.8 2.9 2.6 2.3 0 0 0 0 0 2.3 0 2.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.1 0 4.2 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 3.9 2.7 3.1 4.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 27.3809 33.2447 33.2041 32.4353 31.6006 30.585 37.0162 40.8041 40.4191 37.8774 37.1924 37.1793 40.7605 41.4513 40.1217 37.662 38.8551 38.4272
Actin 0.0021 0.0004 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0009 0.0001 0.0009 0.0001 0.0002 0.0001 0.0001 0.0001 0.0006
Bud 0.0009 0.0001 0 0 0 0.0003 0.0001 0 0.0008 0.0003 0 0.0004 0.0001 0.0006 0.0007 0.0001 0.0004 0.0005
Bud Neck 0.0007 0.0003 0.0002 0.0003 0.0003 0.0006 0.0002 0.0002 0.0003 0.0004 0.0004 0.0008 0.0002 0.0002 0.0002 0.0005 0.0004 0.0007
Bud Periphery 0.0006 0 0 0 0 0.0001 0.0001 0 0.0001 0.0002 0 0.0001 0 0.0001 0.0001 0 0.0001 0.0002
Bud Site 0.0004 0.0001 0 0 0 0 0 0 0.0001 0.0001 0 0 0 0.0003 0.0002 0 0.0001 0
Cell Periphery 0.0001 0.0001 0 0 0 0 0.0001 0.0001 0 0.0001 0 0 0 0.0002 0.0002 0.0001 0.0001 0
Cytoplasm 0.9068 0.9825 0.9823 0.9828 0.9762 0.9625 0.957 0.9833 0.9764 0.9731 0.9674 0.9586 0.9567 0.9751 0.9702 0.9775 0.9796 0.9526
Cytoplasmic Foci 0.0059 0.0003 0.0003 0.0003 0.0005 0.0004 0.0002 0.0002 0.0002 0.0005 0.0003 0.0004 0.0001 0.0006 0.0002 0.0002 0.0005 0.0006
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0 0 0
Endoplasmic Reticulum 0.0036 0.0006 0.0003 0.0006 0.0005 0.0007 0.0009 0.0006 0.0003 0.0006 0.0012 0.0008 0.0009 0.0006 0.001 0.0005 0.0004 0.0011
Endosome 0.0093 0.0001 0.0001 0.0004 0.0002 0.0002 0.0003 0 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003
Golgi 0.0011 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Lipid Particles 0.0009 0 0 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0.0001 0 0 0
Mitochondria 0.0047 0 0 0 0 0.0002 0.0007 0 0.0001 0.0001 0 0.0001 0.0001 0.0006 0.0003 0 0 0.0001
None 0.0042 0.0002 0.0002 0.0001 0.0002 0.0003 0.0004 0.0002 0.0002 0.0011 0.0002 0.0005 0.0003 0.0007 0.0003 0.0002 0.0008 0.0004
Nuclear Periphery 0.0072 0.0025 0.0018 0.0017 0.0029 0.0039 0.0044 0.0017 0.0017 0.002 0.0047 0.0037 0.0045 0.0026 0.0028 0.0023 0.0018 0.0051
Nucleolus 0.0004 0 0 0 0 0 0.0001 0 0 0 0 0 0 0.0024 0.0002 0 0.0001 0.0001
Nucleus 0.0417 0.0119 0.0143 0.0126 0.0182 0.0296 0.0333 0.013 0.0191 0.0195 0.0248 0.0324 0.0361 0.0141 0.0222 0.0176 0.0139 0.0361
Peroxisomes 0.0003 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Punctate Nuclear 0.0019 0.0002 0.0002 0.0002 0.0003 0.0002 0.0002 0.0001 0.0002 0.0006 0.0002 0.0005 0.0001 0.0004 0.0002 0.0002 0.0009 0.0006
Vacuole 0.0051 0.0005 0.0002 0.0007 0.0004 0.0007 0.0013 0.0004 0.0002 0.0004 0.0004 0.0005 0.0005 0.0007 0.0009 0.0006 0.0005 0.0008
Vacuole Periphery 0.0022 0.0001 0 0.0001 0.0001 0.0002 0.0006 0 0 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 239.1838 269.2867 205.8096 142.7671 184.312 168.0125 150.9243 116.3173 104.7273 146.5962
Translational Efficiency 1.9654 2.094 2.044 2.306 2.5772 1.8578 1.961 2.4288 2.1527 2.1575

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1539 1106 2020 1210 1730 1133 1303 491 3269 2239 3323 1701

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2033.06 2769.48 3035.06 3553.67 2297.56 1714.45 2687.83 2039.52 2173.04 2235.60 2898.91 3116.60
Standard Deviation 544.96 686.89 711.58 790.61 527.85 342.61 677.24 358.14 551.99 755.44 718.60 975.92
Intensity Change Log 2 — 0.445962 0.578072 0.805657 — -0.422357 0.226339 -0.171873 — 0.050190 0.402170 0.369098

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000152 0.000089 0.000069 0.000192 0.000172 0.000306 0.000259 0.000195 0.000163 0.000199 0.000144 0.000193
Bud Neck 0.000357 0.000375 0.000716 0.002407 0.000743 0.000539 0.001081 0.001649 0.000561 0.000458 0.000859 0.002188
Bud Site 0.000802 0.000941 0.001392 0.001970 0.002012 0.004291 0.002181 0.003896 0.001442 0.002636 0.001701 0.002526
Cell Periphery 0.000127 0.000246 0.000194 0.000369 0.000176 0.000200 0.000241 0.000441 0.000153 0.000223 0.000212 0.000390
Cytoplasm 0.740144* 0.766629* 0.694777* 0.537784* 0.701699* 0.871898* 0.681016* 0.832737* 0.719799* 0.819898* 0.689381* 0.622924*
Cytoplasmic Foci 0.002943 0.004769 0.004366 0.003884 0.004271 0.013634 0.007693 0.013749 0.003646 0.009255 0.005671 0.006732
Eisosomes 0.000001 0.000001 0.000001 0.000002 0.000001 0.000005 0.000002 0.000002 0.000001 0.000003 0.000001 0.000002
Endoplasmic Reticulum 0.000161 0.000039 0.000076 0.000061 0.000142 0.000690 0.000076 0.000065 0.000151 0.000368 0.000076 0.000062
Endosome 0.000067 0.000214 0.000160 0.000326 0.000453 0.002473 0.001049 0.000521 0.000271 0.001357 0.000508 0.000382
Golgi 0.000293 0.000603 0.000350 0.000101 0.000828 0.002709 0.000805 0.000802 0.000576 0.001669 0.000528 0.000303
Lipid Particles 0.000158 0.000439 0.000279 0.000734 0.000484 0.001326 0.000461 0.001743 0.000331 0.000888 0.000350 0.001025
Mitochondria 0.000200 0.000097 0.000159 0.000217 0.000239 0.000778 0.000183 0.000061 0.000221 0.000442 0.000168 0.000172
Mitotic Spindle 0.000192 0.000936 0.001665 0.002986 0.001167 0.003338 0.004341 0.001717 0.000708 0.002151 0.002714 0.002619
None 0.013395 0.019283 0.019246 0.017968 0.015668 0.003933 0.010790 0.003940 0.014598 0.011515 0.015930 0.013918
Nuclear Periphery 0.000056 0.000060 0.000147 0.000186 0.000121 0.000089 0.000186 0.000119 0.000090 0.000075 0.000163 0.000167
Nuclear Periphery Foci 0.001089 0.001182 0.000780 0.000835 0.002197 0.003733 0.001541 0.002504 0.001675 0.002473 0.001078 0.001317
Nucleolus 0.000083 0.000076 0.000098 0.000209 0.000090 0.000133 0.000123 0.000111 0.000087 0.000105 0.000108 0.000181
Nucleus 0.236208* 0.200279* 0.271498* 0.422634* 0.264325* 0.067851 0.278324* 0.107488 0.251088* 0.133266* 0.274175* 0.331666*
Peroxisomes 0.000010 0.000009 0.000009 0.000014 0.000024 0.000693 0.000057 0.000038 0.000018 0.000355 0.000028 0.000021
Vacuole 0.003154 0.003346 0.003639 0.007086 0.004937 0.018823 0.009301 0.028060 0.004098 0.011178 0.005859 0.013140
Vacuole Periphery 0.000407 0.000391 0.000380 0.000036 0.000251 0.002556 0.000292 0.000163 0.000324 0.001486 0.000345 0.000073

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.28 2.88 -1.44 -1.69 -1.86 -1.95 -2.34 -2.38 0.07 0.71 -0.92 -0.33 -1.58 -1.31 -1.49
Bud Neck -1.77 -7.29 -6.44 -5.75 -4.32 3.79 -5.29 -6.94 -9.46 -2.32 1.66 -7.41 -7.34 -7.81 -4.46
Bud Site -1.29 -2.31 -3.86 -3.34 -2.28 -2.30 -1.34 -6.48 -3.04 -5.43 -2.42 -1.71 -5.25 -2.68 -4.02
Cell Periphery -3.88 -6.75 -11.29 -5.46 -7.08 0.79 -5.37 -4.72 -3.99 -1.28 -1.68 -7.78 -11.50 -7.22 -6.77
Cytoplasm -3.29 11.42 30.90 33.74 21.77 -26.24 8.89 -5.25 11.75 -11.16 -20.48 13.23 21.43 36.21 11.47
Cytoplasmic Foci -4.77 -6.63 -8.42 -3.84 -3.92 -7.36 -7.35 -8.99 -4.44 -4.84 -8.61 -9.12 -11.44 -4.00 -5.31
Eisosomes -0.51 -3.51 -12.40 -11.60 -10.40 -2.58 -3.39 -5.69 0.48 -1.95 -2.57 -3.52 -11.75 -2.85 -8.80
Endoplasmic Reticulum 3.58 1.92 2.36 -3.52 -0.23 -2.01 1.97 2.29 2.30 0.83 -1.50 2.51 3.24 2.11 -0.04
Endosome -1.36 -1.63 -1.69 -0.03 -0.40 -4.12 -0.67 -1.58 3.64 -0.01 -4.25 -0.72 -0.99 3.86 0.12
Golgi -1.89 -1.30 4.73 3.00 4.09 -2.68 0.41 0.62 3.01 0.24 -3.00 0.36 2.41 4.02 3.04
Lipid Particles -2.65 -5.44 -6.25 -3.57 -4.83 -1.51 -1.91 -6.27 -3.51 -5.60 -2.50 -3.50 -7.87 -4.18 -6.73
Mitochondria 1.48 0.03 -0.59 -8.32 -2.27 -1.02 0.10 2.20 1.39 7.63 -0.78 0.13 0.40 0.89 0.88
Mitotic Spindle -1.43 -3.17 -2.97 -1.81 -0.70 -2.23 -3.06 -2.30 -0.24 1.01 -2.58 -3.99 -3.18 -0.99 0.33
None -4.26 -4.36 -0.58 3.47 3.27 10.91 3.97 11.57 1.58 14.88 3.38 -1.15 3.51 0.27 4.98
Nuclear Periphery -2.37 -10.43 -14.87 -13.66 -6.27 3.32 -10.70 -5.97 -7.94 2.84 1.72 -13.51 -14.59 -15.33 -4.24
Nuclear Periphery Foci -1.13 0.56 -1.26 0.09 -1.73 -2.72 0.96 -2.43 0.14 -4.34 -2.76 1.56 -0.94 2.46 -3.56
Nucleolus 0.52 -1.64 -1.60 -1.66 -1.10 -1.02 -4.00 -2.09 0.34 2.43 -0.80 -3.00 -1.63 -1.35 -0.82
Nucleus 5.25 -9.10 -23.08 -28.07 -16.12 37.67* -5.46 22.59 -7.23 26.20 26.74 -9.81 -11.19 -30.11 -4.13
Peroxisomes 0.14 -0.67 -1.79 -1.81 -1.60 -2.02 -1.13 -3.20 1.85 -1.42 -2.02 -0.88 -2.65 1.88 -1.48
Vacuole -1.64 -5.55 -13.15 -12.50 -11.33 -6.53 -7.81 -12.14 -9.53 -9.34 -6.42 -8.05 -16.85 -13.35 -13.59
Vacuole Periphery 0.10 0.23 1.43 1.16 1.32 -2.74 0.07 1.00 2.87 0.76 -2.43 0.12 1.86 3.14 1.54
Standard name
Human Ortholog
Description Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (93%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Lia1

Lia1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lia1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available