Standard name | |
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Human Ortholog | |
Description | Subunit of the NuA4 acetyltransferase complex; this complex acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 1 | 0 | 1 | – | 0 | 2 | 2 | 2 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 3 | 2 | 3 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 3 | 4 | 1 | 8 | – | 13 | 13 | 15 | 15 | 22 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 2 | 7 | 11 | 18 | 5 | – | 18 | 7 | 5 | 3 | 8 | 28 | 35 | 58 | 19 | 6 | 10 | 1 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 2 | 5 | 0 | 0 | 0 | 1 | 0 | 0 |
Endosome | 0 | 1 | 0 | 0 | 2 | 1 | – | 1 | 0 | 0 | 0 | 1 | 6 | 1 | 5 | 3 | 4 | 3 | 0 | 3 | 5 | 1 | 2 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 2 | 0 | 1 | 2 | 1 | 2 | 5 |
Mitochondria | 42 | 11 | 98 | 70 | 13 | 96 | – | 95 | 169 | 150 | 109 | 111 | 9 | 2 | 1 | 11 | 10 | 4 | 1 | 1 | 3 | 0 | 1 | 2 |
Nucleus | 291 | 400 | 233 | 200 | 178 | 271 | – | 314 | 210 | 321 | 171 | 208 | 292 | 227 | 303 | 110 | 49 | 64 | 281 | 369 | 196 | 119 | 163 | 196 |
Nuclear Periphery | 0 | 2 | 0 | 2 | 0 | 8 | – | 4 | 6 | 5 | 3 | 7 | 3 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 29 | 54 | 49 | 57 | 28 | 97 | – | 139 | 138 | 158 | 105 | 116 | 60 | 47 | 58 | 8 | 1 | 10 | 21 | 54 | 38 | 40 | 54 | 53 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 3 | 3 | 0 | 1 | 3 | – | 0 | 3 | 0 | 1 | 0 | 5 | 1 | 3 | 0 | 0 | 0 | 0 | 2 | 2 | 4 | 2 | 7 |
Vac/Vac Membrane | 0 | 1 | 2 | 0 | 2 | 3 | – | 3 | 2 | 1 | 1 | 1 | 3 | 0 | 1 | 14 | 8 | 11 | 3 | 14 | 9 | 6 | 9 | 21 |
Unique Cell Count | 308 | 424 | 264 | 250 | 213 | 356 | 421 | 334 | 430 | 255 | 314 | 353 | 275 | 392 | 153 | 69 | 98 | 311 | 454 | 266 | 181 | 243 | 301 | |
Labelled Cell Count | 366 | 474 | 396 | 345 | 244 | 493 | 587 | 551 | 658 | 410 | 477 | 409 | 315 | 434 | 172 | 82 | 109 | 311 | 454 | 266 | 181 | 243 | 301 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | 7.0 | 4.8 | 5.2 | 6.4 | 4.7 | – | 5.1 | 4.2 | 4.7 | 4.6 | 4.8 | 6.9 | 6.9 | 7.1 | 8.7 | 8.4 | 8.1 | 6.6 | 7.4 | 7.6 |
Std Deviation (1e-4) | 0.9 | 1.3 | 1.4 | 1.6 | 2.5 | 1.2 | – | 1.8 | 1.3 | 1.3 | 1.4 | 1.7 | 1.8 | 1.6 | 1.9 | 1.7 | 1.8 | 1.7 | 1.6 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.42 | -0.03 | – | 0.1 | -0.19 | -0.01 | -0.07 | 0.01 | 0.53 | 0.54 | 0.58 | 0.86 | 0.81 | 0.77 | 0.47 | 0.63 | 0.67 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.1 | 2.8 | -1.1 | – | 1.1 | -0.4 | 0 | 0 | -0.1 | 2.8 | 4.4 | 5.1 | 4.0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -2.2 | -8.0 | 0 | – | -4.1 | 0 | 0 | 0 | 0 | -11.2 | -10.9 | -12.9 | -6.7 | -3.6 | -6.2 |
Nucleus | -2.6 | -1.5 | -3.8 | – | -4.3 | -7.0 | -4.3 | -5.8 | -6.2 | -1.9 | -1.9 | -3.6 | -4.2 | -3.5 | -5.1 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.2 | -1.6 | 0 | – | 4.1 | 0 | 0 | 0 | 0 | -0.5 | -0.4 | -1.3 | -3.8 | -3.5 | -1.9 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.3 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.9073 | 0.642 | 0.4233 | 0.2411 | -0.1548 | 0.7395 | 1.0329 | 0.7097 | 0.7895 | 0.2059 | 1.2048 | 0.8943 | 2.6097 | 2.1798 | 2.1275 | 2.1522 | 2.1587 | 2.0644 |
Actin | 0.0032 | 0 | 0.0106 | 0 | 0.0277 | 0.0001 | 0.0075 | 0 | 0.001 | 0 | 0.0443 | 0.0002 | 0.014 | 0 | 0.0104 | 0 | 0 | 0.0014 |
Bud | 0.0001 | 0 | 0.0001 | 0 | 0.001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0009 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0009 | 0.0002 | 0.0003 | 0.0001 | 0.0171 | 0.0009 | 0.0022 | 0.0002 | 0.0003 | 0.0001 | 0.0066 | 0.0006 | 0.0007 | 0.0001 | 0.0002 | 0 | 0.0005 | 0.0016 |
Bud Periphery | 0.0002 | 0 | 0.0002 | 0 | 0.0016 | 0.0001 | 0.0011 | 0 | 0.0002 | 0 | 0.0022 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 |
Bud Site | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0086 | 0.0001 | 0.0008 | 0.0001 | 0.0004 | 0 | 0.0105 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cell Periphery | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0048 | 0.0008 | 0.0001 | 0 | 0.0019 | 0.0004 | 0.0008 | 0.0021 | 0.0024 | 0 | 0.0031 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 |
Cytoplasmic Foci | 0.0016 | 0 | 0.0003 | 0 | 0.0018 | 0 | 0.0023 | 0.0001 | 0.0011 | 0 | 0.0024 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Eisosomes | 0 | 0 | 0 | 0 | 0.0007 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0008 | 0.0001 | 0.0001 | 0 | 0.0018 | 0.0002 | 0.0012 | 0.0001 | 0.0014 | 0 | 0.003 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0.0005 |
Endosome | 0.0036 | 0.0001 | 0.0005 | 0 | 0.0065 | 0.0009 | 0.0065 | 0.0003 | 0.0023 | 0 | 0.0132 | 0.0017 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0.0018 |
Golgi | 0.0004 | 0 | 0.001 | 0 | 0.0022 | 0 | 0.003 | 0 | 0.0005 | 0 | 0.0112 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0.0005 |
Lipid Particles | 0.0006 | 0 | 0.0008 | 0 | 0.0017 | 0.0001 | 0.0083 | 0 | 0.0045 | 0 | 0.0081 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0003 |
Mitochondria | 0.0017 | 0.0001 | 0.0002 | 0.0003 | 0.0019 | 0.0003 | 0.008 | 0.0001 | 0.0011 | 0.0001 | 0.0085 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 |
None | 0.0056 | 0.0109 | 0.0001 | 0 | 0.0052 | 0.0001 | 0.0009 | 0.0003 | 0.0027 | 0 | 0.0068 | 0.0003 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0.0001 |
Nuclear Periphery | 0.0109 | 0.0019 | 0.0009 | 0.0005 | 0.0044 | 0.0035 | 0.0075 | 0.0028 | 0.0101 | 0.0002 | 0.0323 | 0.0032 | 0.0012 | 0.0006 | 0.0002 | 0.0004 | 0.0008 | 0.0061 |
Nucleolus | 0.0277 | 0.0275 | 0.0159 | 0.0101 | 0.0731 | 0.0471 | 0.0231 | 0.0225 | 0.0169 | 0.0101 | 0.0604 | 0.0554 | 0.0245 | 0.0412 | 0.0306 | 0.0163 | 0.0891 | 0.0614 |
Nucleus | 0.919 | 0.9514 | 0.9664 | 0.9886 | 0.8258 | 0.9366 | 0.9139 | 0.9506 | 0.945 | 0.9878 | 0.7405 | 0.9307 | 0.9546 | 0.9493 | 0.9569 | 0.9827 | 0.9051 | 0.9192 |
Peroxisomes | 0.0008 | 0 | 0.0004 | 0 | 0.0011 | 0 | 0.0024 | 0 | 0.0029 | 0 | 0.0014 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 |
Punctate Nuclear | 0.0162 | 0.0066 | 0.0017 | 0.0001 | 0.0133 | 0.009 | 0.007 | 0.0205 | 0.0058 | 0.0016 | 0.0403 | 0.0067 | 0.0016 | 0.0083 | 0.0006 | 0.0003 | 0.0039 | 0.0051 |
Vacuole | 0.0009 | 0.0001 | 0 | 0.0001 | 0.0015 | 0.0004 | 0.0016 | 0.0002 | 0.0008 | 0 | 0.0016 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0.0009 |
Vacuole Periphery | 0.0007 | 0 | 0 | 0.0001 | 0.0006 | 0.0002 | 0.0009 | 0.0001 | 0.0005 | 0 | 0.0017 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | – |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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