Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 19 | 21 | 18 | 17 | 14 | 6 | 19 | 10 | 15 | 10 | 9 | 2 | 3 | 1 | 0 | 4 | 3 | 7 | 29 | 33 | 8 | 19 | 23 |
Bud | 1 | 7 | 0 | 8 | 11 | 11 | 11 | 15 | 31 | 16 | 13 | 21 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 12 | 5 | 2 | 9 | 12 |
Bud Neck | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 24 | 20 | 9 | 13 | 15 |
Bud Site | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 14 | 28 | 12 | 5 | 16 | 16 | 24 | 18 | 14 | 20 | 17 | 19 | 30 | 13 | 12 | 24 | 5 | 0 | 3 | 7 | 0 | 4 | 5 |
Cytoplasm | 141 | 477 | 378 | 261 | 188 | 326 | 282 | 451 | 298 | 272 | 239 | 216 | 365 | 678 | 608 | 366 | 342 | 247 | 139 | 439 | 326 | 298 | 373 | 428 |
Endoplasmic Reticulum | 8 | 25 | 17 | 13 | 16 | 25 | 4 | 23 | 6 | 17 | 5 | 4 | 9 | 5 | 2 | 19 | 13 | 12 | 5 | 12 | 27 | 7 | 34 | 20 |
Endosome | 0 | 4 | 9 | 13 | 0 | 18 | 7 | 7 | 9 | 13 | 7 | 7 | 1 | 0 | 0 | 6 | 10 | 5 | 6 | 8 | 10 | 4 | 7 | 6 |
Golgi | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 1 | 0 | 0 | 1 |
Mitochondria | 6 | 12 | 6 | 31 | 36 | 80 | 61 | 51 | 235 | 128 | 170 | 152 | 0 | 0 | 0 | 11 | 4 | 3 | 2 | 17 | 29 | 19 | 26 | 21 |
Nucleus | 0 | 0 | 1 | 1 | 7 | 0 | 7 | 11 | 3 | 4 | 0 | 2 | 1 | 0 | 0 | 2 | 5 | 5 | 0 | 1 | 1 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 2 | 1 | 2 | 0 | 2 | 4 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 7 | 4 | 2 | 9 | 1 | 10 | 2 | 4 | 3 | 3 | 0 | 1 | 0 | 2 | 2 | 3 | 0 | 3 | 4 | 1 | 0 | 5 |
Vac/Vac Membrane | 0 | 2 | 12 | 13 | 6 | 39 | 52 | 90 | 56 | 39 | 24 | 22 | 1 | 0 | 0 | 18 | 17 | 11 | 0 | 14 | 3 | 7 | 3 | 7 |
Unique Cell Count | 160 | 536 | 434 | 329 | 245 | 457 | 386 | 635 | 520 | 440 | 386 | 367 | 377 | 684 | 611 | 395 | 377 | 270 | 179 | 579 | 479 | 366 | 499 | 561 |
Labelled Cell Count | 174 | 563 | 481 | 378 | 291 | 543 | 449 | 707 | 678 | 531 | 493 | 458 | 398 | 717 | 624 | 437 | 422 | 296 | 179 | 579 | 479 | 366 | 499 | 561 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 5.6 | 7.4 | 6.8 | 6.0 | 6.4 | 6.1 | 6.4 | 5.4 | 5.8 | 5.5 | 5.6 | 7.2 | 7.1 | 6.8 | 6.7 | 8.2 | 7.5 | 7.2 | 7.7 | 8.3 |
Std Deviation (1e-4) | 0.8 | 0.7 | 1.1 | 1.4 | 1.5 | 1.4 | 1.4 | 1.0 | 1.2 | 1.2 | 1.2 | 1.1 | 0.9 | 0.9 | 1.0 | 1.2 | 1.6 | 1.3 | 1.4 | 1.2 | 5.2 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.29 | -0.2 | -0.26 | -0.19 | -0.45 | -0.33 | -0.41 | -0.41 | -0.02 | -0.05 | -0.12 | -0.14 | 0.16 | 0.02 | -0.03 | 0.07 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.4 | 1.1 | -1.4 | -2.6 | -1.6 | -2.5 | -1.1 | -1.7 | -1.8 | -3.7 | -4.9 | -5.2 | -4.4 | -3.1 | -2.7 |
Bud | 0 | 0 | 3.3 | 3.5 | 3.2 | 5.2 | 4.0 | 3.9 | 5.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.7 | -2.6 | -2.0 | -1.5 | -2.0 | -2.1 | -2.3 | -0.8 | -1.1 | -0.9 | -1.5 | -3.5 | -2.3 | 0 | -2.8 |
Cytoplasm | -2.9 | -3.5 | -5.8 | -5.1 | -6.2 | -10.1 | -8.6 | -8.3 | -9.1 | 5.0 | 8.6 | 8.6 | 2.6 | 1.6 | 1.8 |
Endoplasmic Reticulum | 0 | 1.5 | 1.1 | -2.6 | -0.2 | -2.8 | 0 | -2.3 | -2.5 | -1.2 | -3.7 | -4.3 | 0.6 | -0.4 | 0.3 |
Endosome | 1.5 | 0 | 1.6 | -0.3 | -1.3 | -0.4 | 0.8 | -0.3 | -0.2 | 0 | 0 | 0 | -0.6 | 0.5 | -0.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.1 | 6.9 | 8.1 | 7.5 | 4.8 | 15.5 | 11.4 | 14.9 | 14.2 | 0 | 0 | 0 | 1.4 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0.4 | 0 | 0 | 0 | -0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.9 | -0.2 | 3.7 | 5.7 | 6.2 | 4.8 | 3.8 | 2.4 | 2.3 | -2.8 | 0 | 0 | 1.4 | 1.3 | 1.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.2482 | 6.2062 | 6.7072 | 6.2817 | 6.247 | 6.6079 | 8.7123 | 7.4213 | 8.2119 | 7.5305 | 7.8086 | 8.4766 | 5.3029 | 4.8848 | 4.801 | 4.5195 | 4.5697 | 5.1155 |
Actin | 0.0664 | 0.0028 | 0.0175 | 0.0026 | 0.002 | 0.0296 | 0.0462 | 0.0097 | 0.0169 | 0.0037 | 0.0186 | 0.0237 | 0.0157 | 0.0019 | 0.007 | 0.0092 | 0.0012 | 0.0119 |
Bud | 0.0013 | 0.0018 | 0.0045 | 0.0045 | 0.001 | 0.0025 | 0.0028 | 0.0055 | 0.0023 | 0.0091 | 0.0055 | 0.0031 | 0.0016 | 0.0019 | 0.0037 | 0.0007 | 0.0007 | 0.0012 |
Bud Neck | 0.0089 | 0.0012 | 0.0025 | 0.0013 | 0.0024 | 0.0983 | 0.0175 | 0.0014 | 0.0054 | 0.0007 | 0.0052 | 0.0879 | 0.0068 | 0.0008 | 0.0042 | 0.0003 | 0.0389 | 0.0855 |
Bud Periphery | 0.0028 | 0.0012 | 0.0024 | 0.012 | 0.0009 | 0.0063 | 0.0047 | 0.0058 | 0.0028 | 0.0102 | 0.0094 | 0.0049 | 0.003 | 0.002 | 0.0051 | 0.0008 | 0.0006 | 0.0017 |
Bud Site | 0.0131 | 0.0407 | 0.012 | 0.0016 | 0.0008 | 0.0038 | 0.0191 | 0.0458 | 0.0195 | 0.0147 | 0.0229 | 0.0022 | 0.0254 | 0.0314 | 0.0281 | 0.001 | 0.0004 | 0.0023 |
Cell Periphery | 0.0077 | 0.0026 | 0.002 | 0.0011 | 0.0004 | 0.0027 | 0.0064 | 0.0046 | 0.0031 | 0.0016 | 0.0013 | 0.002 | 0.0071 | 0.0034 | 0.0026 | 0.0006 | 0.0003 | 0.0011 |
Cytoplasm | 0.7102 | 0.8419 | 0.8032 | 0.8419 | 0.8096 | 0.7535 | 0.6711 | 0.8112 | 0.7927 | 0.887 | 0.7035 | 0.7676 | 0.7992 | 0.8388 | 0.8256 | 0.9018 | 0.9202 | 0.7546 |
Cytoplasmic Foci | 0.0323 | 0.0104 | 0.02 | 0.0146 | 0.0078 | 0.0195 | 0.0381 | 0.0203 | 0.0224 | 0.0071 | 0.0395 | 0.0225 | 0.0133 | 0.0173 | 0.0134 | 0.0157 | 0.0105 | 0.0262 |
Eisosomes | 0.001 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0018 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0261 | 0.0043 | 0.005 | 0.0072 | 0.019 | 0.0079 | 0.018 | 0.0056 | 0.0088 | 0.0124 | 0.0064 | 0.0106 | 0.0136 | 0.004 | 0.0061 | 0.0032 | 0.0039 | 0.0087 |
Endosome | 0.0295 | 0.0047 | 0.011 | 0.0139 | 0.0071 | 0.0092 | 0.0441 | 0.0135 | 0.026 | 0.0099 | 0.0759 | 0.015 | 0.0072 | 0.0147 | 0.0136 | 0.0166 | 0.0079 | 0.0147 |
Golgi | 0.0162 | 0.0014 | 0.0054 | 0.0027 | 0.001 | 0.0056 | 0.0205 | 0.0069 | 0.0076 | 0.0011 | 0.0307 | 0.0097 | 0.0032 | 0.0024 | 0.0025 | 0.0016 | 0.001 | 0.0071 |
Lipid Particles | 0.0077 | 0.0003 | 0.0018 | 0.0002 | 0.0001 | 0.0009 | 0.0114 | 0.0009 | 0.0077 | 0.0007 | 0.0027 | 0.0005 | 0.0072 | 0.0003 | 0.0006 | 0.0008 | 0 | 0.0005 |
Mitochondria | 0.0047 | 0.0003 | 0.0025 | 0.0006 | 0.0003 | 0.0021 | 0.0141 | 0.0024 | 0.0046 | 0.0004 | 0.0172 | 0.0021 | 0.0017 | 0.0005 | 0.0009 | 0.0004 | 0.0007 | 0.0014 |
None | 0.0398 | 0.0821 | 0.0977 | 0.0896 | 0.1435 | 0.0498 | 0.043 | 0.0565 | 0.0639 | 0.0257 | 0.0171 | 0.0419 | 0.0826 | 0.0728 | 0.0752 | 0.0371 | 0.0101 | 0.0779 |
Nuclear Periphery | 0.0109 | 0.0004 | 0.0006 | 0.0006 | 0.0006 | 0.0005 | 0.0118 | 0.0008 | 0.001 | 0.0023 | 0.013 | 0.0005 | 0.0012 | 0.0005 | 0.0011 | 0.0005 | 0.0002 | 0.0004 |
Nucleolus | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0007 | 0.001 | 0.0003 | 0.0004 | 0.0003 | 0.002 | 0.0001 | 0.0011 | 0.0002 | 0.0002 | 0 | 0 | 0.0001 |
Nucleus | 0.0035 | 0.0009 | 0.001 | 0.0011 | 0.0009 | 0.0011 | 0.0077 | 0.0018 | 0.0012 | 0.0028 | 0.01 | 0.0008 | 0.0015 | 0.0015 | 0.0016 | 0.0007 | 0.0005 | 0.0007 |
Peroxisomes | 0.0094 | 0.0002 | 0.0065 | 0.0002 | 0.0001 | 0.0025 | 0.0095 | 0.0009 | 0.0054 | 0.0001 | 0.0028 | 0.0022 | 0.0015 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0015 |
Punctate Nuclear | 0.003 | 0.0003 | 0.0008 | 0.0005 | 0.0002 | 0.0017 | 0.0045 | 0.0011 | 0.0028 | 0.0014 | 0.0036 | 0.0004 | 0.0014 | 0.0005 | 0.0006 | 0.0005 | 0.0001 | 0.0009 |
Vacuole | 0.0037 | 0.0024 | 0.0025 | 0.0034 | 0.002 | 0.0017 | 0.0064 | 0.0044 | 0.0046 | 0.0081 | 0.0103 | 0.0016 | 0.0037 | 0.0044 | 0.0068 | 0.0069 | 0.002 | 0.0014 |
Vacuole Periphery | 0.0011 | 0.0002 | 0.0003 | 0.0005 | 0.0003 | 0.0003 | 0.0018 | 0.0004 | 0.0006 | 0.0007 | 0.0022 | 0.0003 | 0.0003 | 0.0004 | 0.0008 | 0.0014 | 0.0003 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.8646 | 13.7881 | 7.0832 | 7.8346 | 9.9989 | 11.858 | 13.1368 | 18.4567 | 8.123 | 10.6602 |
Translational Efficiency | 1.6938 | 1.3397 | 2.1869 | 1.5791 | 1.8236 | 1.6569 | 1.4999 | 1.0082 | 2.2102 | 1.643 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
167 | 1791 | 2854 | 1236 | 1642 | 1534 | 2591 | 1723 | 1809 | 3325 | 5445 | 2959 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 894.15 | 899.05 | 908.72 | 921.23 | 719.52 | 919.35 | 878.15 | 956.56 | 735.64 | 908.42 | 894.17 | 941.80 |
Standard Deviation | 119.35 | 127.63 | 110.93 | 126.05 | 84.67 | 122.33 | 112.28 | 125.04 | 101.87 | 125.62 | 112.61 | 126.67 |
Intensity Change Log 2 | — | 0.007884 | 0.023319 | 0.043045 | — | 0.353579 | 0.287433 | 0.410821 | — | 0.172324 | 0.147089 | 0.218690 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002837 | 0.006075 | 0.003775 | 0.003234 | 0.002704 | 0.003245 | 0.002096 | 0.001867 | 0.002716 | 0.004770 | 0.002976 | 0.002438 |
Bud Neck | 0.027973 | 0.020882 | 0.029641 | 0.007526 | 0.034162 | 0.039314 | 0.020244 | 0.010227 | 0.033591 | 0.029385 | 0.025170 | 0.009098 |
Bud Site | 0.073251 | 0.130758* | 0.062682 | 0.069169 | 0.060334 | 0.065637 | 0.041443 | 0.054970 | 0.061527 | 0.100714* | 0.052575 | 0.060901 |
Cell Periphery | 0.001645 | 0.000693 | 0.000156 | 0.000311 | 0.000438 | 0.000577 | 0.000169 | 0.000127 | 0.000549 | 0.000639 | 0.000162 | 0.000204 |
Cytoplasm | 0.398105* | 0.497156* | 0.673445* | 0.581190* | 0.399719* | 0.415962* | 0.582010* | 0.670417* | 0.399570* | 0.459697* | 0.629936* | 0.633146* |
Cytoplasmic Foci | 0.277085* | 0.227593* | 0.061016 | 0.079446 | 0.306134* | 0.221468* | 0.062662 | 0.067345 | 0.303452* | 0.224767* | 0.061799 | 0.072400 |
Eisosomes | 0.001360 | 0.000535 | 0.000087 | 0.000109 | 0.000770 | 0.000731 | 0.000101 | 0.000050 | 0.000824 | 0.000626 | 0.000094 | 0.000075 |
Endoplasmic Reticulum | 0.003699 | 0.002720 | 0.000619 | 0.000454 | 0.003375 | 0.003487 | 0.000761 | 0.000286 | 0.003405 | 0.003074 | 0.000686 | 0.000356 |
Endosome | 0.053788 | 0.018075 | 0.004572 | 0.009882 | 0.043023 | 0.050658 | 0.009695 | 0.006486 | 0.044017 | 0.033107 | 0.007010 | 0.007904 |
Golgi | 0.026992 | 0.033125 | 0.001400 | 0.003981 | 0.047372 | 0.020682 | 0.000697 | 0.001950 | 0.045490 | 0.027384 | 0.001066 | 0.002798 |
Lipid Particles | 0.008580 | 0.002903 | 0.001289 | 0.000732 | 0.011228 | 0.009574 | 0.002380 | 0.000591 | 0.010984 | 0.005980 | 0.001808 | 0.000650 |
Mitochondria | 0.001937 | 0.004991 | 0.000582 | 0.001581 | 0.011374 | 0.010017 | 0.000419 | 0.000734 | 0.010503 | 0.007310 | 0.000504 | 0.001088 |
Mitotic Spindle | 0.001496 | 0.001405 | 0.003981 | 0.025052 | 0.000640 | 0.005405 | 0.004409 | 0.016885 | 0.000719 | 0.003250 | 0.004185 | 0.020296 |
None | 0.000086 | 0.000716 | 0.000756 | 0.000380 | 0.001561 | 0.001704 | 0.001297 | 0.000794 | 0.001425 | 0.001172 | 0.001013 | 0.000621 |
Nuclear Periphery | 0.000109 | 0.000028 | 0.000130 | 0.000409 | 0.000058 | 0.000167 | 0.000218 | 0.000396 | 0.000062 | 0.000092 | 0.000172 | 0.000402 |
Nuclear Periphery Foci | 0.000864 | 0.000278 | 0.000539 | 0.001062 | 0.000223 | 0.000294 | 0.000580 | 0.000926 | 0.000282 | 0.000285 | 0.000558 | 0.000983 |
Nucleolus | 0.000152 | 0.000158 | 0.000210 | 0.000106 | 0.000296 | 0.000588 | 0.000484 | 0.000119 | 0.000283 | 0.000356 | 0.000340 | 0.000114 |
Nucleus | 0.008042 | 0.005452 | 0.048354 | 0.009077 | 0.009322 | 0.018835 | 0.057715 | 0.013084 | 0.009204 | 0.011626 | 0.052808 | 0.011410 |
Peroxisomes | 0.010442 | 0.007294 | 0.008453 | 0.006383 | 0.009735 | 0.009883 | 0.004508 | 0.005574 | 0.009800 | 0.008488 | 0.006576 | 0.005912 |
Vacuole | 0.098107 | 0.035233 | 0.097947 | 0.196106 | 0.052085 | 0.115436 | 0.207588* | 0.146532 | 0.056333 | 0.072235 | 0.150120 | 0.167239 |
Vacuole Periphery | 0.003450 | 0.003930 | 0.000367 | 0.003808 | 0.005450 | 0.006337 | 0.000525 | 0.000640 | 0.005266 | 0.005041 | 0.000442 | 0.001963 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.33 | -0.61 | 1.05 | 5.14 | 2.63 | -0.86 | 2.18 | 3.84 | 3.01 | 1.44 | -4.00 | 0.32 | 3.56 | 6.15 | 3.26 |
Bud Neck | 0.91 | 0.41 | 2.97 | 10.56 | 11.49 | -1.74 | 6.78 | 12.05 | 14.22 | 7.06 | 1.78 | 5.41 | 13.01 | 17.20 | 13.01 |
Bud Site | -5.00 | 1.90 | 2.42 | 15.36 | 1.54 | -1.23 | 6.89 | 5.86 | 6.56 | -1.03 | -9.96 | 5.35 | 5.86 | 16.94 | 0.99 |
Cell Periphery | 3.33 | 5.38 | 5.07 | 6.24 | -1.00 | -1.69 | 6.78 | 8.11 | 6.71 | 4.03 | -1.42 | 8.70 | 7.85 | 9.91 | 0.22 |
Cytoplasm | -5.53 | -14.31 | -8.29 | -5.34 | 9.80 | -1.70 | -21.00 | -24.83 | -22.16 | -6.10 | -7.88 | -30.58 | -24.87 | -19.41 | 2.57 |
Cytoplasmic Foci | 3.42 | 15.53 | 14.71 | 29.01 | -2.84 | 11.94 | 45.06 | 43.05 | 28.87 | -0.41 | 13.36 | 48.76 | 46.30 | 41.17 | -2.17 |
Eisosomes | 2.22 | 3.51 | 3.52 | 6.05 | 0.26 | 0.35 | 12.96 | 13.97 | 6.85 | 6.60 | 2.37 | 12.71 | 13.08 | 9.27 | 2.91 |
Endoplasmic Reticulum | 0.89 | 2.93 | 3.25 | 6.37 | 5.31 | -0.16 | 7.09 | 8.47 | 5.47 | 5.25 | 0.68 | 7.74 | 8.94 | 8.22 | 7.63 |
Endosome | 6.28 | 8.81 | 8.25 | 10.52 | -3.87 | -2.71 | 18.01 | 20.51 | 20.74 | 4.63 | 5.32 | 21.56 | 21.68 | 23.05 | 0.64 |
Golgi | -1.41 | 6.12 | 5.79 | 19.50 | -1.68 | 13.28 | 28.02 | 27.02 | 16.47 | -1.68 | 9.90 | 28.32 | 27.28 | 25.67 | -2.15 |
Lipid Particles | 3.94 | 5.05 | 5.49 | 11.37 | 4.18 | 1.41 | 10.65 | 13.03 | 10.61 | 4.97 | 5.92 | 12.22 | 13.80 | 13.04 | 6.35 |
Mitochondria | -4.58 | 3.06 | 1.62 | 6.33 | -0.96 | 0.97 | 12.11 | 11.87 | 8.92 | -0.64 | 3.21 | 12.16 | 11.61 | 11.27 | -1.08 |
Mitotic Spindle | 0.05 | -1.76 | -5.33 | -5.79 | -4.92 | -4.06 | -4.08 | -6.54 | -3.84 | -4.56 | -3.91 | -6.33 | -8.89 | -7.09 | -6.75 |
None | -5.26 | -8.11 | -5.24 | 2.12 | 3.16 | -0.18 | 0.70 | 1.42 | 2.04 | 1.30 | 0.49 | 0.97 | 1.59 | 2.39 | 2.01 |
Nuclear Periphery | 1.54 | -1.66 | -1.66 | -2.39 | -1.12 | -4.90 | -5.94 | -1.63 | -1.00 | -0.63 | -2.26 | -9.16 | -2.43 | -2.17 | -1.12 |
Nuclear Periphery Foci | 3.82 | 1.47 | -1.65 | -8.41 | -5.00 | -1.24 | -7.99 | -3.10 | -2.71 | -0.97 | -0.06 | -9.44 | -5.42 | -5.30 | -2.54 |
Nucleolus | 0.05 | -1.28 | 0.53 | 1.03 | 3.27 | -2.43 | -1.42 | 3.39 | 4.05 | 2.67 | -1.05 | -0.88 | 3.49 | 4.16 | 3.29 |
Nucleus | 1.51 | -20.66 | -3.32 | -11.96 | 24.13 | -7.51 | -25.38 | -9.75 | -0.09 | 18.77 | -3.25 | -36.31 | -10.47 | -6.45 | 29.53 |
Peroxisomes | 0.83 | 0.83 | 1.58 | 3.97 | 3.46 | -0.18 | 8.79 | 7.69 | 6.58 | -0.41 | 1.88 | 5.98 | 7.91 | 7.63 | 2.66 |
Vacuole | 5.73 | -2.71 | -12.96 | -31.88 | -17.74 | -13.49 | -34.96 | -26.51 | -13.28 | 2.23 | -5.20 | -34.01 | -36.04 | -32.52 | -11.26 |
Vacuole Periphery | -0.82 | 5.73 | 1.33 | 2.11 | -2.27 | -1.43 | 15.37 | 14.77 | 10.90 | 0.25 | 0.56 | 16.36 | 9.12 | 8.78 | -2.19 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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