Standard name | |
---|---|
Human Ortholog | |
Description | Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 22 | 14 | 24 | 21 | 18 | 18 | 26 | 17 | 30 | 10 | 23 | 4 | 18 | 21 | 14 | 15 | 14 | 1 | 6 | 5 | 5 | 5 | 5 |
Bud | 1 | 2 | 0 | 1 | 1 | 1 | 3 | 1 | 5 | 10 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 7 | 9 | 13 | 15 | 12 | 10 | 20 | 13 | 23 | 7 | 15 | 8 | 55 | 67 | 45 | 27 | 39 | 0 | 0 | 1 | 1 | 3 | 7 |
Cytoplasm | 10 | 35 | 9 | 12 | 22 | 26 | 37 | 32 | 25 | 42 | 28 | 44 | 4 | 19 | 13 | 29 | 18 | 26 | 2 | 12 | 1 | 1 | 4 | 3 |
Endoplasmic Reticulum | 189 | 258 | 174 | 137 | 215 | 178 | 226 | 175 | 159 | 252 | 112 | 179 | 95 | 237 | 242 | 4 | 4 | 2 | 202 | 287 | 176 | 149 | 239 | 277 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 5 | 3 | 23 | 16 | 29 | 2 | 4 | 13 | 1 | 6 | 0 | 8 | 5 | 4 | 5 | 27 | 16 | 17 | 0 | 2 | 0 | 0 | 1 | 5 |
Mitochondria | 2 | 1 | 12 | 18 | 22 | 25 | 48 | 37 | 55 | 70 | 47 | 45 | 0 | 0 | 2 | 11 | 10 | 19 | 3 | 4 | 3 | 1 | 4 | 9 |
Nucleus | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 2 | 1 | 1 | 1 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 2 | 11 | 9 | 4 | 5 | 9 | 9 | 14 | 16 | 8 | 12 | 4 | 2 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 8 | 4 | 3 | 8 | 14 | 43 | 53 | 21 | 52 | 23 | 48 | 2 | 0 | 5 | 80 | 32 | 53 | 3 | 7 | 0 | 2 | 4 | 5 |
Unique Cell Count | 200 | 298 | 187 | 168 | 253 | 232 | 328 | 299 | 232 | 396 | 184 | 308 | 108 | 283 | 296 | 170 | 104 | 139 | 218 | 328 | 192 | 167 | 267 | 325 |
Labelled Cell Count | 218 | 339 | 257 | 233 | 338 | 282 | 401 | 368 | 313 | 502 | 240 | 379 | 125 | 336 | 358 | 211 | 125 | 174 | 218 | 328 | 192 | 167 | 267 | 325 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.7 | 16.4 | 17.5 | 17.0 | 15.6 | 13.1 | 12.3 | 13.7 | 12.5 | 12.5 | 12.0 | 12.3 | 17.2 | 17.2 | 17.5 | 13.8 | 13.8 | 13.9 | 15.8 | 15.8 | 17.0 |
Std Deviation (1e-4) | 2.9 | 2.8 | 3.0 | 2.7 | 2.8 | 2.5 | 2.3 | 2.8 | 2.8 | 2.5 | 2.6 | 2.9 | 3.2 | 2.9 | 2.7 | 5.4 | 5.4 | 5.3 | 2.5 | 2.7 | 2.9 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.17 | -0.42 | -0.51 | -0.35 | -0.49 | -0.48 | -0.55 | -0.51 | -0.03 | -0.03 | -0.0 | -0.34 | -0.34 | -0.33 | -0.15 | -0.15 | -0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.1 | 0.3 | 0.1 | -0.9 | 0.5 | -0.1 | 0 | -0.8 | 0 | -1.3 | -0.5 | -0.2 | 0.3 | 1.9 | 0.8 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.1 | 0.5 | 0.2 | -1.0 | 0.8 | 0.4 | 0.5 | -0.5 | 0 | 0.9 | 4.5 | 5.2 | 5.7 | 5.3 | 5.9 |
Cytoplasm | 0.9 | 1.6 | 2.4 | 2.5 | 2.3 | 2.2 | 2.3 | 3.3 | 3.3 | 0 | 0.9 | -0.2 | 3.7 | 3.5 | 4.0 |
Endoplasmic Reticulum | -3.3 | -2.6 | -4.5 | -6.3 | -8.2 | -6.2 | -7.5 | -7.4 | -8.3 | -1.5 | -3.0 | -3.5 | -17.1 | -15.0 | -16.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -0.8 | -0.3 | -4.9 | -5.4 | -3.3 | -5.2 | -5.6 | -4.9 | -4.3 | -2.2 | -5.0 | -4.9 | 1.0 | 0.7 | 0 |
Mitochondria | 1.5 | 0.9 | 1.6 | 2.8 | 2.1 | 4.8 | 3.7 | 5.0 | 2.8 | 0 | 0 | -3.7 | 0 | 1.0 | 2.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -0.2 | -2.5 | -2.0 | -1.8 | -1.6 | 0.1 | -1.0 | -0.7 | -1.0 | -0.8 | -3.3 | -3.4 | -3.2 | 0 | -2.0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0.7 | 2.0 | 4.2 | 5.2 | 3.0 | 4.2 | 3.8 | 4.7 | 0 | 0 | 0 | 10.0 | 7.1 | 8.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 12.4041 | 11.0448 | 11.3515 | 9.9678 | 11.2468 | 10.8605 | 13.6691 | 14.4624 | 13.1252 | 12.1436 | 10.6418 | 13.5536 | 9.749 | 13.6203 | 10.5922 | 10.7434 | 9.552 | 11.0576 |
Actin | 0.0093 | 0.0133 | 0.0015 | 0.0008 | 0.0014 | 0.0011 | 0.0181 | 0.0001 | 0.0101 | 0.0032 | 0.0388 | 0.0017 | 0.0396 | 0.0011 | 0.0014 | 0 | 0.0086 | 0.0003 |
Bud | 0 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0007 | 0 | 0 | 0 | 0.0003 | 0 |
Bud Neck | 0.0001 | 0.0012 | 0.0007 | 0.0002 | 0.0004 | 0.0006 | 0.0004 | 0.0001 | 0.0018 | 0.0012 | 0.0003 | 0.0006 | 0.0009 | 0.0003 | 0.0001 | 0 | 0.0005 | 0.0004 |
Bud Periphery | 0.0001 | 0.0006 | 0.0005 | 0.0001 | 0 | 0.0002 | 0.0003 | 0 | 0.0005 | 0.001 | 0.0004 | 0.0001 | 0.0008 | 0 | 0.0001 | 0 | 0.0003 | 0 |
Bud Site | 0.0001 | 0.0034 | 0.0004 | 0 | 0 | 0 | 0.004 | 0 | 0.0014 | 0.0009 | 0.0008 | 0 | 0.0128 | 0.0002 | 0 | 0 | 0.0006 | 0 |
Cell Periphery | 0.0018 | 0.0149 | 0.0044 | 0.0017 | 0.0007 | 0.0012 | 0.001 | 0.0021 | 0.0011 | 0.0041 | 0.0008 | 0.0004 | 0.0012 | 0.0029 | 0.0014 | 0.0008 | 0.0002 | 0.0006 |
Cytoplasm | 0.0109 | 0.0373 | 0.0153 | 0.004 | 0.0029 | 0.0054 | 0.0103 | 0.0061 | 0.0034 | 0.002 | 0.0021 | 0.007 | 0.0085 | 0.1013 | 0.0078 | 0.001 | 0.0025 | 0.0018 |
Cytoplasmic Foci | 0.002 | 0.002 | 0.0006 | 0.0015 | 0.001 | 0.0003 | 0.0081 | 0.0003 | 0.0033 | 0.0014 | 0.0071 | 0.0038 | 0.0082 | 0.0025 | 0.0009 | 0.0001 | 0.0113 | 0.0002 |
Eisosomes | 0.0001 | 0.0008 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0006 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.9102 | 0.8855 | 0.9596 | 0.9319 | 0.9625 | 0.9663 | 0.8987 | 0.9813 | 0.9364 | 0.924 | 0.8328 | 0.9522 | 0.8025 | 0.8629 | 0.949 | 0.9918 | 0.7903 | 0.9823 |
Endosome | 0.0054 | 0.0048 | 0.0023 | 0.0045 | 0.0128 | 0.0051 | 0.0164 | 0.0007 | 0.0096 | 0.008 | 0.0276 | 0.0069 | 0.0426 | 0.0032 | 0.0068 | 0.0009 | 0.0607 | 0.0019 |
Golgi | 0.0017 | 0.0063 | 0.0013 | 0.0024 | 0.0055 | 0.0012 | 0.0046 | 0.0016 | 0.0049 | 0.0057 | 0.0289 | 0.0046 | 0.0239 | 0.0008 | 0.0215 | 0.0019 | 0.0839 | 0.0006 |
Lipid Particles | 0.0024 | 0.0143 | 0.002 | 0.0231 | 0.0007 | 0.0014 | 0.0108 | 0.004 | 0.0034 | 0.0106 | 0.0077 | 0.0027 | 0.0098 | 0.0093 | 0.0005 | 0.0003 | 0.0013 | 0.0005 |
Mitochondria | 0.0002 | 0.0032 | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0.0007 | 0 | 0.0146 | 0.0252 | 0.0162 | 0.0013 | 0.0068 | 0 | 0.0015 | 0.0001 | 0.0299 | 0.0001 |
None | 0.0007 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0012 | 0 | 0.0002 | 0.0002 | 0.001 | 0.0048 | 0.0012 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Nuclear Periphery | 0.036 | 0.0071 | 0.0061 | 0.0258 | 0.0097 | 0.0129 | 0.012 | 0.0024 | 0.0037 | 0.0031 | 0.0093 | 0.0071 | 0.0239 | 0.0089 | 0.0064 | 0.0019 | 0.0053 | 0.0076 |
Nucleolus | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0006 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0077 | 0.0008 | 0.0005 | 0.0007 | 0.0002 | 0.0006 | 0.0006 | 0.0001 | 0.0003 | 0.0003 | 0.0005 | 0.0004 | 0.0063 | 0.0006 | 0.0002 | 0 | 0.0002 | 0.0003 |
Peroxisomes | 0.0049 | 0.0001 | 0 | 0 | 0 | 0 | 0.0074 | 0 | 0.0005 | 0.0006 | 0.0205 | 0.0032 | 0.0016 | 0 | 0 | 0 | 0.0006 | 0 |
Punctate Nuclear | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0004 | 0.0001 | 0.0005 | 0.0003 | 0.0038 | 0.0002 | 0 | 0 | 0.0001 | 0 |
Vacuole | 0.0045 | 0.003 | 0.0029 | 0.0016 | 0.0012 | 0.0028 | 0.0029 | 0.001 | 0.003 | 0.0059 | 0.0018 | 0.002 | 0.0029 | 0.0054 | 0.0017 | 0.0008 | 0.0012 | 0.0028 |
Vacuole Periphery | 0.0012 | 0.0005 | 0.0006 | 0.0007 | 0.0005 | 0.0006 | 0.0011 | 0.0001 | 0.0007 | 0.0014 | 0.0022 | 0.0008 | 0.0014 | 0.0003 | 0.0006 | 0.0001 | 0.0019 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 47.0736 | 42.642 | 42.4671 | 53.8511 | 59.4054 | 37.638 | 48.7883 | 55.5195 | 68.9542 | 54.9252 |
Translational Efficiency | 1.7203 | 2.0105 | 2.0736 | 2.1407 | 1.4193 | 1.9603 | 1.9628 | 1.9062 | 1.3424 | 1.4656 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
203 | 1346 | 2320 | 440 | 1360 | 1206 | 1494 | 1984 | 1563 | 2552 | 3814 | 2424 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1278.39 | 1348.00 | 1655.23 | 2190.91 | 1226.86 | 1353.85 | 1754.51 | 2062.79 | 1233.55 | 1350.76 | 1694.12 | 2086.05 |
Standard Deviation | 204.81 | 220.80 | 283.85 | 442.41 | 187.60 | 217.58 | 321.11 | 346.06 | 190.72 | 219.30 | 302.90 | 368.76 |
Intensity Change Log 2 | — | 0.076492 | 0.372704 | 0.777202 | — | 0.142097 | 0.516098 | 0.749626 | — | 0.108993 | 0.444707 | 0.763764 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000456 | 0.001310 | 0.000830 | 0.000576 | 0.001749 | 0.001118 | 0.002073 | 0.001192 | 0.001581 | 0.001219 | 0.001317 | 0.001080 |
Bud Neck | 0.000168 | 0.000192 | 0.000985 | 0.010095 | 0.000132 | 0.000160 | 0.001759 | 0.001174 | 0.000137 | 0.000177 | 0.001288 | 0.002793 |
Bud Site | 0.000865 | 0.002503 | 0.004993 | 0.022180 | 0.001724 | 0.001618 | 0.012289 | 0.008913 | 0.001613 | 0.002085 | 0.007851 | 0.011321 |
Cell Periphery | 0.002370 | 0.001996 | 0.002017 | 0.041905 | 0.002047 | 0.001799 | 0.005063 | 0.013529 | 0.002089 | 0.001903 | 0.003210 | 0.018679 |
Cytoplasm | 0.005323 | 0.002285 | 0.024980 | 0.006541 | 0.002282 | 0.001149 | 0.022114 | 0.009483 | 0.002677 | 0.001748 | 0.023858 | 0.008949 |
Cytoplasmic Foci | 0.004188 | 0.003832 | 0.010996 | 0.005887 | 0.004629 | 0.003873 | 0.014155 | 0.003904 | 0.004572 | 0.003851 | 0.012233 | 0.004264 |
Eisosomes | 0.000043 | 0.000060 | 0.000067 | 0.000388 | 0.000085 | 0.000120 | 0.000155 | 0.000281 | 0.000079 | 0.000088 | 0.000102 | 0.000300 |
Endoplasmic Reticulum | 0.945546* | 0.956169* | 0.866911* | 0.761287* | 0.962920* | 0.961002* | 0.818092* | 0.896475* | 0.960664* | 0.958453* | 0.847788* | 0.871936* |
Endosome | 0.013679 | 0.010407 | 0.013032 | 0.014197 | 0.008106 | 0.007257 | 0.023774 | 0.008685 | 0.008830 | 0.008918 | 0.017240 | 0.009686 |
Golgi | 0.009736 | 0.006566 | 0.006884 | 0.022755 | 0.003737 | 0.004342 | 0.017263 | 0.009088 | 0.004516 | 0.005515 | 0.010950 | 0.011569 |
Lipid Particles | 0.007009 | 0.005371 | 0.025918 | 0.055277 | 0.003752 | 0.005135 | 0.035234 | 0.023826 | 0.004175 | 0.005260 | 0.029567 | 0.029535 |
Mitochondria | 0.002537 | 0.000559 | 0.001045 | 0.000991 | 0.001041 | 0.001480 | 0.002660 | 0.002615 | 0.001235 | 0.000995 | 0.001677 | 0.002320 |
Mitotic Spindle | 0.000335 | 0.000083 | 0.001762 | 0.001271 | 0.000126 | 0.000851 | 0.001391 | 0.000467 | 0.000153 | 0.000446 | 0.001617 | 0.000613 |
None | 0.000161 | 0.000795 | 0.002122 | 0.000446 | 0.000479 | 0.000350 | 0.001649 | 0.002015 | 0.000438 | 0.000585 | 0.001937 | 0.001730 |
Nuclear Periphery | 0.000313 | 0.000314 | 0.002996 | 0.004655 | 0.000268 | 0.000417 | 0.001337 | 0.000841 | 0.000274 | 0.000363 | 0.002346 | 0.001533 |
Nuclear Periphery Foci | 0.004262 | 0.002771 | 0.021056 | 0.011603 | 0.002730 | 0.002903 | 0.017173 | 0.003641 | 0.002929 | 0.002833 | 0.019535 | 0.005086 |
Nucleolus | 0.000005 | 0.000006 | 0.000090 | 0.000069 | 0.000182 | 0.000161 | 0.000038 | 0.000036 | 0.000159 | 0.000079 | 0.000070 | 0.000042 |
Nucleus | 0.000078 | 0.000077 | 0.004216 | 0.003697 | 0.000290 | 0.000152 | 0.000671 | 0.000331 | 0.000262 | 0.000112 | 0.002827 | 0.000942 |
Peroxisomes | 0.000188 | 0.000263 | 0.000356 | 0.000050 | 0.000452 | 0.000347 | 0.000531 | 0.001229 | 0.000417 | 0.000303 | 0.000425 | 0.001015 |
Vacuole | 0.001649 | 0.002888 | 0.008095 | 0.028222 | 0.001621 | 0.002788 | 0.018762 | 0.009210 | 0.001625 | 0.002840 | 0.012273 | 0.012661 |
Vacuole Periphery | 0.001090 | 0.001553 | 0.000650 | 0.007907 | 0.001648 | 0.002981 | 0.003816 | 0.003068 | 0.001576 | 0.002228 | 0.001890 | 0.003946 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.80 | -2.16 | -0.90 | 1.37 | 1.07 | 0.79 | -0.51 | 0.70 | -0.23 | 2.07 | 0.53 | 0.35 | 0.71 | 0.25 | 0.86 |
Bud Neck | -0.53 | -5.25 | -6.25 | -6.24 | -5.79 | -0.65 | -6.87 | -5.30 | -5.16 | 1.91 | -1.25 | -8.69 | -7.77 | -7.67 | -4.48 |
Bud Site | -3.11 | -5.20 | -5.46 | -5.09 | -4.50 | 0.20 | -7.19 | -5.98 | -5.98 | 1.66 | -1.10 | -7.95 | -7.99 | -7.62 | -2.92 |
Cell Periphery | 0.59 | 0.51 | -8.61 | -8.75 | -8.74 | 0.49 | -4.58 | -11.53 | -12.78 | -8.34 | 0.43 | -2.48 | -13.88 | -14.72 | -13.42 |
Cytoplasm | 0.95 | -5.49 | -0.63 | -2.30 | 5.80 | 2.86 | -10.81 | -6.50 | -7.68 | 5.79 | 1.71 | -14.80 | -6.07 | -7.56 | 8.55 |
Cytoplasmic Foci | 0.23 | -3.95 | -1.05 | -1.42 | 2.10 | 0.66 | -5.97 | 0.61 | -0.20 | 6.60 | 0.85 | -7.16 | 0.03 | -0.90 | 7.54 |
Eisosomes | -1.32 | -1.96 | -3.96 | -3.77 | -3.68 | -0.83 | -2.54 | -1.76 | -1.40 | -1.14 | -0.34 | -1.09 | -2.38 | -2.29 | -2.17 |
Endoplasmic Reticulum | -0.94 | 6.75 | 10.20 | 12.52 | 6.80 | 0.38 | 18.45 | 11.87 | 10.63 | -9.05 | 0.58 | 21.10 | 15.67 | 15.99 | -3.30 |
Endosome | 1.10 | 0.15 | -0.30 | -2.56 | -0.84 | 0.93 | -11.65 | -0.87 | -1.91 | 11.58 | -0.13 | -8.91 | -1.25 | -1.36 | 8.82 |
Golgi | 0.79 | 0.71 | -2.55 | -4.85 | -4.83 | -0.61 | -8.48 | -4.82 | -3.69 | 4.54 | -1.07 | -6.43 | -5.84 | -5.09 | -0.43 |
Lipid Particles | 0.73 | -6.42 | -6.96 | -7.49 | -4.23 | -1.06 | -11.14 | -9.58 | -8.25 | 3.47 | -1.19 | -14.19 | -11.88 | -11.41 | 0.07 |
Mitochondria | 1.01 | 0.75 | 0.75 | -1.08 | 0.03 | -0.49 | -2.54 | -2.22 | -1.17 | 0.05 | 0.41 | -0.94 | -1.70 | -2.17 | -1.20 |
Mitotic Spindle | 0.78 | -2.60 | -2.36 | -4.32 | 0.87 | -1.12 | -3.33 | -2.62 | 0.36 | 1.92 | -0.94 | -4.50 | -3.55 | -0.87 | 2.55 |
None | -2.08 | -4.11 | -1.97 | 1.00 | 3.43 | 0.79 | -3.20 | -2.96 | -3.19 | -0.61 | -0.80 | -4.59 | -3.05 | -2.59 | 0.36 |
Nuclear Periphery | -0.04 | -5.68 | -8.15 | -8.21 | -2.33 | -1.52 | -4.93 | -5.99 | -3.83 | 2.27 | -1.26 | -6.96 | -9.81 | -9.12 | 2.61 |
Nuclear Periphery Foci | 1.10 | -7.62 | -3.44 | -4.68 | 3.21 | -0.18 | -8.17 | -1.30 | -0.99 | 7.49 | 0.13 | -11.95 | -2.97 | -3.40 | 9.90 |
Nucleolus | -0.26 | -2.39 | -4.16 | -4.15 | 0.52 | 0.08 | 0.80 | 0.81 | 0.82 | 0.14 | 0.46 | 0.52 | 0.74 | 0.48 | 1.21 |
Nucleus | -0.01 | -5.95 | -2.51 | -2.51 | -0.09 | 0.57 | -1.75 | -0.29 | -2.09 | 3.44 | 0.74 | -5.56 | -2.25 | -3.04 | 3.32 |
Peroxisomes | -0.75 | -2.36 | 2.34 | 2.57 | 6.84 | 0.80 | -0.61 | -3.95 | -4.24 | -3.38 | 1.21 | -0.10 | -3.66 | -4.41 | -3.79 |
Vacuole | -1.96 | -6.99 | -7.81 | -7.45 | -5.88 | -1.66 | -10.00 | -8.47 | -6.35 | 5.26 | -2.49 | -12.07 | -11.34 | -9.85 | -0.29 |
Vacuole Periphery | -0.58 | 0.87 | -3.83 | -3.47 | -4.22 | -1.43 | -2.90 | -2.13 | -0.14 | 1.02 | -0.97 | -0.66 | -3.64 | -2.50 | -3.83 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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