Standard name | |
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Human Ortholog | |
Description | Member of the Puf family of RNA-binding proteins; interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl; JSN1 has a paralog, PUF2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 23 | 44 | 34 | 68 | 40 | 30 | 27 | 12 | 11 | 20 | 11 | 31 | 85 | 122 | 13 | 4 | 8 | 16 | 42 | 49 | 18 | 31 | 33 |
Bud | 3 | 10 | 22 | 19 | 34 | 20 | 21 | 33 | 23 | 16 | 14 | 24 | 13 | 28 | 46 | 6 | 2 | 2 | 2 | 8 | 4 | 1 | 5 | 2 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 25 | 34 | 10 | 23 | 16 |
Bud Site | 0 | 0 | 1 | 3 | 1 | 3 | 3 | 3 | 3 | 1 | 0 | 2 | 0 | 3 | 4 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 21 | 63 | 67 | 61 | 62 | 88 | 72 | 66 | 50 | 65 | 41 | 64 | 114 | 159 | 174 | 117 | 56 | 73 | 23 | 37 | 60 | 17 | 40 | 93 |
Cytoplasm | 26 | 76 | 140 | 77 | 78 | 125 | 183 | 239 | 152 | 192 | 85 | 205 | 92 | 113 | 100 | 81 | 42 | 62 | 18 | 48 | 55 | 57 | 122 | 97 |
Endoplasmic Reticulum | 4 | 15 | 21 | 6 | 24 | 32 | 27 | 25 | 13 | 9 | 36 | 7 | 3 | 13 | 18 | 4 | 3 | 4 | 12 | 18 | 44 | 9 | 28 | 28 |
Endosome | 0 | 0 | 0 | 0 | 3 | 0 | 4 | 5 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 0 | 2 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 3 | 3 |
Mitochondria | 1 | 5 | 6 | 22 | 32 | 72 | 102 | 156 | 124 | 159 | 109 | 155 | 0 | 3 | 30 | 6 | 1 | 3 | 1 | 6 | 16 | 0 | 4 | 2 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 2 | 1 | 1 | 0 | 1 | 1 | 0 | 2 | 7 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 3 | 2 | 9 | 10 | 2 | 8 | 2 | 26 | 0 | 1 | 1 | 11 | 8 | 8 | 0 | 6 | 8 | 4 | 7 | 1 |
Unique Cell Count | 48 | 149 | 235 | 168 | 218 | 262 | 341 | 437 | 275 | 339 | 235 | 362 | 175 | 280 | 313 | 184 | 91 | 122 | 84 | 200 | 282 | 123 | 275 | 284 |
Labelled Cell Count | 64 | 195 | 304 | 222 | 312 | 384 | 460 | 569 | 381 | 465 | 308 | 499 | 254 | 408 | 500 | 248 | 120 | 170 | 84 | 200 | 282 | 123 | 275 | 284 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.4 | 7.1 | 5.8 | 5.7 | 6.1 | 5.4 | 5.0 | 5.0 | 4.5 | 4.4 | 4.3 | 4.2 | 8.2 | 8.2 | 7.3 | 11.9 | 13.4 | 13.1 | 7.7 | 8.6 | 9.4 |
Std Deviation (1e-4) | 1.4 | 1.1 | 1.1 | 1.0 | 1.2 | 0.9 | 0.9 | 1.0 | 1.1 | 0.9 | 0.8 | 1.1 | 1.1 | 1.3 | 1.4 | 2.4 | 2.6 | 2.7 | 1.3 | 1.6 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.09 | -0.09 | -0.19 | -0.19 | -0.36 | -0.4 | -0.42 | -0.45 | 0.51 | 0.5 | 0.34 | 1.05 | 1.22 | 1.19 | 0.42 | 0.58 | 0.7 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.4 | 3.1 | -1.0 | -3.5 | -5.0 | 0 | 0 | 0 | 0 | -0.3 | 3.0 | 5.1 | -3.5 | -3.3 | -3.1 |
Bud | 0.6 | 2.0 | -0.7 | -1.4 | -0.8 | 0 | 0 | 0 | 0 | -0.7 | 0.2 | 1.9 | -2.5 | -2.2 | -2.8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.7 | 0 | 1.2 | -2.0 | -4.2 | 0 | 0 | 0 | 0 | 7.4 | 6.4 | 6.3 | 7.2 | 5.5 | 5.7 |
Cytoplasm | -2.7 | -5.1 | -2.6 | -1.4 | -1.2 | -1.0 | -0.7 | -5.1 | -0.7 | -1.4 | -4.3 | -6.5 | -3.2 | -2.2 | -1.6 |
Endoplasmic Reticulum | -2.1 | 0.7 | 1.2 | -0.4 | -1.6 | 0 | 0 | 0 | 0 | -3.1 | -2.0 | -1.4 | -2.9 | -1.7 | -2.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.1 | 4.7 | 7.6 | 8.3 | 9.6 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0.4 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.2 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.8177 | 5.5218 | 5.0703 | 4.7758 | 4.1385 | 5.1564 | 6.0951 | 6.0795 | 6.269 | 5.7542 | 5.103 | 5.7241 | 7.5662 | 6.8819 | 6.8866 | 6.7652 | 7.1567 | 7.5059 |
Actin | 0.1732 | 0.0347 | 0.0872 | 0.1262 | 0.1015 | 0.0827 | 0.0352 | 0.0391 | 0.0539 | 0.0302 | 0.0222 | 0.1242 | 0.0775 | 0.0082 | 0.1364 | 0.1459 | 0.1066 | 0.0407 |
Bud | 0.0032 | 0.0018 | 0.0049 | 0.0057 | 0.0032 | 0.002 | 0.0012 | 0.0015 | 0.0024 | 0.0047 | 0.0013 | 0.0027 | 0.0006 | 0.0011 | 0.004 | 0.0046 | 0.0014 | 0.0029 |
Bud Neck | 0.0191 | 0.0079 | 0.0329 | 0.0302 | 0.0311 | 0.136 | 0.0345 | 0.0158 | 0.0307 | 0.0674 | 0.1 | 0.0966 | 0.0072 | 0.014 | 0.0231 | 0.0441 | 0.0338 | 0.0812 |
Bud Periphery | 0.0088 | 0.0064 | 0.0347 | 0.0509 | 0.0263 | 0.0214 | 0.0047 | 0.0043 | 0.0201 | 0.0456 | 0.0086 | 0.0275 | 0.0034 | 0.0026 | 0.0281 | 0.07 | 0.0098 | 0.0263 |
Bud Site | 0.0285 | 0.1813 | 0.0818 | 0.0105 | 0.0039 | 0.0031 | 0.0675 | 0.2085 | 0.069 | 0.0087 | 0.0345 | 0.0062 | 0.0439 | 0.258 | 0.0499 | 0.0058 | 0.0032 | 0.0064 |
Cell Periphery | 0.1734 | 0.1599 | 0.1815 | 0.249 | 0.1855 | 0.1776 | 0.2993 | 0.1761 | 0.2347 | 0.1645 | 0.1453 | 0.2505 | 0.3418 | 0.1107 | 0.2373 | 0.275 | 0.1873 | 0.2761 |
Cytoplasm | 0.2335 | 0.5811 | 0.4735 | 0.4306 | 0.5682 | 0.5325 | 0.4382 | 0.5293 | 0.5241 | 0.4869 | 0.5776 | 0.4214 | 0.4287 | 0.6005 | 0.4585 | 0.4075 | 0.5335 | 0.4933 |
Cytoplasmic Foci | 0.0404 | 0.0019 | 0.008 | 0.0043 | 0.0111 | 0.0022 | 0.0349 | 0.0054 | 0.004 | 0.012 | 0.0129 | 0.0037 | 0.0061 | 0.0005 | 0.0074 | 0.0045 | 0.0007 | 0.0043 |
Eisosomes | 0.0046 | 0.0028 | 0.0017 | 0.0018 | 0.0023 | 0.0037 | 0.0047 | 0.0024 | 0.0028 | 0.0009 | 0.0003 | 0.0045 | 0.0282 | 0.0002 | 0.0021 | 0.0051 | 0.0088 | 0.0012 |
Endoplasmic Reticulum | 0.0652 | 0.0074 | 0.02 | 0.032 | 0.0103 | 0.0187 | 0.0308 | 0.006 | 0.015 | 0.017 | 0.0045 | 0.0079 | 0.0201 | 0.0018 | 0.0087 | 0.0064 | 0.0061 | 0.0184 |
Endosome | 0.081 | 0.0015 | 0.0092 | 0.0111 | 0.0066 | 0.0019 | 0.0088 | 0.0006 | 0.0101 | 0.0133 | 0.0383 | 0.0096 | 0.0077 | 0.0001 | 0.0039 | 0.0019 | 0.0001 | 0.0048 |
Golgi | 0.0592 | 0.0041 | 0.0151 | 0.0189 | 0.0127 | 0.0037 | 0.0045 | 0.0023 | 0.0039 | 0.026 | 0.0477 | 0.019 | 0.0084 | 0.0003 | 0.0123 | 0.0206 | 0.0006 | 0.0076 |
Lipid Particles | 0.0403 | 0.0011 | 0.0086 | 0.0057 | 0.0199 | 0.0029 | 0.0044 | 0.0013 | 0.0021 | 0.0035 | 0.0012 | 0.0012 | 0.017 | 0.0002 | 0.0111 | 0.0036 | 0.0004 | 0.0017 |
Mitochondria | 0.0149 | 0.0002 | 0.0257 | 0.0133 | 0.0027 | 0.0056 | 0.001 | 0.0015 | 0.0004 | 0.064 | 0.0011 | 0.0045 | 0.0009 | 0 | 0.0058 | 0.0005 | 0 | 0.0116 |
None | 0.0049 | 0.0046 | 0.0044 | 0.0008 | 0.0013 | 0.0011 | 0.0183 | 0.0029 | 0.0224 | 0.0439 | 0.0009 | 0.0179 | 0.0029 | 0.0002 | 0.0013 | 0.0009 | 0.1064 | 0.0011 |
Nuclear Periphery | 0.0128 | 0.0007 | 0.0014 | 0.0019 | 0.0004 | 0.0012 | 0.0019 | 0.0004 | 0.001 | 0.001 | 0.0002 | 0.0003 | 0.0012 | 0.0002 | 0.0007 | 0.0004 | 0.0004 | 0.0006 |
Nucleolus | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 |
Nucleus | 0.0034 | 0.0006 | 0.0008 | 0.0008 | 0.0004 | 0.0006 | 0.0011 | 0.0004 | 0.0005 | 0.0007 | 0.0004 | 0.0003 | 0.0003 | 0.0005 | 0.0003 | 0.0003 | 0.0002 | 0.0007 |
Peroxisomes | 0.0153 | 0.0001 | 0.0042 | 0.003 | 0.0093 | 0.0002 | 0.0032 | 0.001 | 0.0003 | 0.0054 | 0.0004 | 0.0005 | 0.0013 | 0 | 0.0069 | 0.0013 | 0 | 0.0177 |
Punctate Nuclear | 0.0038 | 0.0002 | 0.0006 | 0.0004 | 0.0003 | 0.0001 | 0.0035 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0006 | 0.0002 | 0.0001 | 0.0001 |
Vacuole | 0.0106 | 0.0016 | 0.0032 | 0.0024 | 0.0024 | 0.002 | 0.0017 | 0.0012 | 0.002 | 0.0026 | 0.0024 | 0.001 | 0.0018 | 0.0008 | 0.0012 | 0.0014 | 0.0006 | 0.0028 |
Vacuole Periphery | 0.0029 | 0.0001 | 0.0004 | 0.0005 | 0.0002 | 0.0007 | 0.0002 | 0 | 0.0002 | 0.0016 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.0718 | 21.6786 | 29.045 | 40.8205 | 24.0182 | 25.5837 | 35.6948 | 41.2999 | 39.5234 | 38.4637 |
Translational Efficiency | 0.81 | 0.9132 | 0.8098 | 0.5067 | 0.6558 | 0.9616 | 0.6252 | 0.5998 | 0.7003 | 0.6377 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1661 | 1401 | 122 | 881 | 1813 | 1959 | 86 | 81 | 3474 | 3360 | 208 | 962 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 843.82 | 971.36 | 1596.30 | 1213.92 | 881.73 | 963.66 | 1381.38 | 1210.19 | 863.60 | 966.87 | 1507.44 | 1213.61 |
Standard Deviation | 110.63 | 139.64 | 178.42 | 168.27 | 129.13 | 139.72 | 140.44 | 165.86 | 122.11 | 139.74 | 195.01 | 168.07 |
Intensity Change Log 2 | — | 0.203071 | 0.919725 | 0.524666 | — | 0.128187 | 0.647701 | 0.456825 | — | 0.165292 | 0.787132 | 0.490399 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.026932 | 0.035647 | 0.093078 | 0.076473 | 0.022809 | 0.054166 | 0.103190* | 0.093957 | 0.024781 | 0.046444 | 0.097259 | 0.077945 |
Bud Neck | 0.054999 | 0.051535 | 0.029789 | 0.017901 | 0.045471 | 0.072679 | 0.023104 | 0.014614 | 0.050027 | 0.063863 | 0.027025 | 0.017624 |
Bud Site | 0.197697* | 0.225109* | 0.241981* | 0.210628* | 0.160185* | 0.231291* | 0.376343* | 0.252490* | 0.178120* | 0.228714* | 0.297535* | 0.214153* |
Cell Periphery | 0.237802* | 0.112567* | 0.073931 | 0.034784 | 0.030756 | 0.069544 | 0.057624 | 0.026446 | 0.129749* | 0.087483 | 0.067189 | 0.034082 |
Cytoplasm | 0.366821* | 0.453797* | 0.230889* | 0.467252* | 0.576000* | 0.440089* | 0.204448* | 0.423471* | 0.475987* | 0.445804* | 0.219957* | 0.463566* |
Cytoplasmic Foci | 0.023181 | 0.034499 | 0.069160 | 0.039444 | 0.065572 | 0.034906 | 0.051394 | 0.049370 | 0.045304 | 0.034737 | 0.061815 | 0.040280 |
Eisosomes | 0.017269 | 0.008879 | 0.001715 | 0.003271 | 0.007841 | 0.016190 | 0.002898 | 0.002526 | 0.012349 | 0.013142 | 0.002204 | 0.003208 |
Endoplasmic Reticulum | 0.015899 | 0.018286 | 0.032083 | 0.003183 | 0.012183 | 0.008828 | 0.026126 | 0.002819 | 0.013960 | 0.012771 | 0.029620 | 0.003153 |
Endosome | 0.004148 | 0.007264 | 0.011859 | 0.005191 | 0.008955 | 0.006939 | 0.007642 | 0.001993 | 0.006657 | 0.007075 | 0.010116 | 0.004922 |
Golgi | 0.009741 | 0.012172 | 0.009704 | 0.003036 | 0.016475 | 0.011675 | 0.006281 | 0.001306 | 0.013255 | 0.011882 | 0.008289 | 0.002891 |
Lipid Particles | 0.005064 | 0.003989 | 0.061579 | 0.010946 | 0.008791 | 0.003987 | 0.018495 | 0.014456 | 0.007009 | 0.003988 | 0.043765 | 0.011241 |
Mitochondria | 0.009863 | 0.004648 | 0.000023 | 0.000441 | 0.005969 | 0.007018 | 0.000075 | 0.000038 | 0.007831 | 0.006030 | 0.000045 | 0.000407 |
Mitotic Spindle | 0.000288 | 0.000607 | 0.000890 | 0.002712 | 0.000285 | 0.002713 | 0.000247 | 0.000110 | 0.000287 | 0.001835 | 0.000624 | 0.002493 |
None | 0.000376 | 0.000154 | 0.000056 | 0.000254 | 0.001728 | 0.000215 | 0.000023 | 0.000039 | 0.001081 | 0.000189 | 0.000042 | 0.000236 |
Nuclear Periphery | 0.000078 | 0.000034 | 0.000125 | 0.000164 | 0.000059 | 0.000053 | 0.000057 | 0.000202 | 0.000068 | 0.000045 | 0.000097 | 0.000167 |
Nuclear Periphery Foci | 0.000320 | 0.000347 | 0.017350 | 0.002615 | 0.000396 | 0.000274 | 0.006727 | 0.004830 | 0.000360 | 0.000304 | 0.012958 | 0.002802 |
Nucleolus | 0.000058 | 0.000052 | 0.000020 | 0.000057 | 0.000187 | 0.000142 | 0.000015 | 0.000285 | 0.000125 | 0.000104 | 0.000018 | 0.000077 |
Nucleus | 0.001975 | 0.002537 | 0.007135 | 0.008273 | 0.006625 | 0.004745 | 0.005565 | 0.013117 | 0.004402 | 0.003824 | 0.006486 | 0.008681 |
Peroxisomes | 0.020124 | 0.008349 | 0.001862 | 0.004522 | 0.008478 | 0.007607 | 0.002111 | 0.004973 | 0.014046 | 0.007916 | 0.001965 | 0.004560 |
Vacuole | 0.006134 | 0.017816 | 0.116476 | 0.107714 | 0.019579 | 0.024230 | 0.107468 | 0.092814 | 0.013151 | 0.021556 | 0.112752 | 0.106460 |
Vacuole Periphery | 0.001231 | 0.001713 | 0.000294 | 0.001138 | 0.001654 | 0.002709 | 0.000165 | 0.000144 | 0.001452 | 0.002293 | 0.000241 | 0.001054 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.78 | -6.24 | -9.48 | -7.32 | 1.78 | -11.49 | -6.04 | -4.03 | -1.95 | 0.84 | -11.62 | -8.75 | -10.91 | -5.51 | 2.60 |
Bud Neck | 1.05 | 6.94 | 14.04 | 12.88 | 3.78 | -8.21 | 5.73 | 12.16 | 19.29 | 2.23 | -5.79 | 8.72 | 16.68 | 21.42 | 3.78 |
Bud Site | -3.33 | -1.93 | 0.32 | 3.33 | 2.04 | -10.12 | -7.05 | -3.03 | 0.02 | 3.59 | -9.40 | -6.53 | -2.62 | 4.01 | 5.06 |
Cell Periphery | 15.00 | 13.46 | 28.61* | 14.80 | 3.49 | -10.77 | -2.22 | 1.51 | 8.37 | 2.72 | 9.02 | 8.05 | 22.24 | 15.72 | 4.12 |
Cytoplasm | -9.31 | 6.94 | -9.22 | -1.16 | -11.57 | 16.11 | 16.67 | 5.19 | 0.38 | -6.41 | 4.64 | 17.43 | 1.25 | -1.87 | -14.89 |
Cytoplasmic Foci | -4.66 | -6.57 | -6.56 | -2.63 | 3.76 | 9.52 | 1.20 | 1.22 | -1.82 | 0.02 | 5.08 | -3.26 | 0.24 | -3.22 | 3.19 |
Eisosomes | 7.64 | 15.95 | 14.00 | 9.56 | -3.40 | -8.26 | 7.59 | 8.25 | 14.50 | 0.90 | -1.01 | 17.70 | 15.11 | 16.20 | -2.02 |
Endoplasmic Reticulum | -1.08 | -1.75 | 9.30 | 7.98 | 3.35 | 2.10 | -1.86 | 6.84 | 5.13 | 3.33 | 0.92 | -2.52 | 10.45 | 8.71 | 4.54 |
Endosome | -4.45 | -6.37 | -0.95 | 2.12 | 4.72 | 2.35 | 0.53 | 9.20 | 7.43 | 2.13 | -0.74 | -2.57 | 2.17 | 2.60 | 3.55 |
Golgi | -1.85 | 0.50 | 5.51 | 6.27 | 4.47 | 3.74 | 3.03 | 15.70 | 11.93 | 1.35 | 1.51 | 3.36 | 9.38 | 7.92 | 2.88 |
Lipid Particles | 2.19 | -7.41 | -4.91 | -6.01 | 6.57 | 6.20 | -1.68 | -1.49 | -3.02 | 0.72 | 6.42 | -6.96 | -3.61 | -6.65 | 6.06 |
Mitochondria | 5.30 | 10.96 | 10.39 | 9.87 | -2.39 | -0.99 | 8.69 | 8.75 | 8.55 | 0.76 | 2.40 | 13.97 | 12.98 | 10.80 | -2.24 |
Mitotic Spindle | -0.79 | -4.14 | -2.90 | -2.31 | -2.20 | -2.57 | 0.23 | 1.16 | 2.78 | 2.09 | -2.69 | -2.93 | -2.88 | -0.77 | -2.46 |
None | 4.31 | 7.01 | 0.77 | -1.70 | -2.89 | 2.51 | 2.83 | 2.80 | 4.30 | -1.52 | 2.83 | 3.30 | 2.47 | -1.15 | -3.07 |
Nuclear Periphery | 8.56 | -3.91 | -0.96 | -1.53 | -0.29 | 0.28 | -0.36 | -0.86 | -0.90 | -0.83 | 2.06 | -2.85 | -1.23 | -1.54 | -0.74 |
Nuclear Periphery Foci | -1.18 | -9.10 | -5.44 | -5.38 | 7.63 | 1.96 | -4.43 | -2.98 | -3.06 | 1.04 | 1.60 | -9.71 | -5.97 | -6.10 | 7.41 |
Nucleolus | 0.87 | 6.10 | -0.39 | -0.88 | -3.40 | 0.74 | 5.31 | -0.39 | -0.54 | -1.00 | 0.60 | 6.23 | 1.37 | 0.57 | -2.45 |
Nucleus | -3.55 | -6.18 | -9.34 | -8.59 | -1.23 | 3.64 | 0.02 | -0.87 | -1.10 | -0.87 | 1.95 | -3.85 | -5.27 | -5.83 | -1.96 |
Peroxisomes | 9.03 | 15.16 | 11.88 | 5.01 | -4.50 | 1.25 | 9.69 | 3.11 | 2.51 | -2.17 | 8.44 | 17.99 | 11.50 | 5.54 | -4.63 |
Vacuole | -9.60 | -9.87 | -19.62 | -17.57 | -0.30 | -3.02 | -6.23 | -5.74 | -5.46 | 0.36 | -8.28 | -11.27 | -19.42 | -17.93 | -0.25 |
Vacuole Periphery | -2.29 | 7.12 | 0.43 | 1.35 | -1.48 | -1.63 | 14.97 | 13.18 | 4.00 | 0.26 | -2.18 | 14.83 | 1.12 | 2.31 | -1.57 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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