Standard name | |
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Human Ortholog | |
Description | Anillin-like protein involved in bud-site selection; required for the axial budding pattern; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; functions as a platform linking the cytokinesis tag septins to the axial landmark through its multiple domains; in vivo substrate of Cdc28p/Clb2p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 23 | 9 | 20 | 16 | 11 | 20 | 20 | 19 | 4 | 7 | 5 | 1 | 15 | 10 | 17 | 4 | 1 | 4 | 10 | 11 | 16 | 2 | 13 | 3 |
Bud | 41 | 25 | 67 | 46 | 55 | 58 | 74 | 85 | 62 | 79 | 50 | 59 | 12 | 18 | 17 | 9 | 3 | 3 | 128 | 36 | 69 | 21 | 7 | 7 |
Bud Neck | 76 | 20 | 69 | 36 | 69 | 48 | 50 | 52 | 37 | 37 | 34 | 34 | 10 | 15 | 13 | 23 | 9 | 11 | 247 | 96 | 164 | 38 | 15 | 1 |
Bud Site | 129 | 29 | 111 | 116 | 78 | 96 | 78 | 88 | 69 | 64 | 46 | 47 | 46 | 32 | 61 | 33 | 8 | 13 | – | – | – | – | – | – |
Cell Periphery | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 35 | 24 | 37 | 41 | 55 | 56 | 70 | 88 | 76 | 102 | 54 | 75 | 23 | 30 | 38 | 43 | 8 | 9 | 9 | 7 | 10 | 55 | 170 | 185 |
Endoplasmic Reticulum | 0 | 1 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 5 | 3 | 1 | 0 | 0 | 2 | 0 | 3 | 1 |
Endosome | 8 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 5 | 2 | 9 | 5 | 4 | 7 | 8 | 6 | 0 | 2 | 1 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 3 |
Mitochondria | 10 | 35 | 53 | 75 | 36 | 103 | 117 | 172 | 146 | 172 | 114 | 140 | 11 | 5 | 16 | 10 | 4 | 7 | 6 | 7 | 12 | 2 | 4 | 4 |
Nucleus | 45 | 13 | 35 | 14 | 16 | 15 | 21 | 35 | 14 | 29 | 18 | 14 | 4 | 3 | 3 | 13 | 3 | 2 | 1 | 3 | 4 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 5 | 4 | 2 | 3 | 5 | 0 | 3 | 4 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 6 | 7 | 0 | 0 | 0 |
Nucleolus | 21 | 13 | 7 | 2 | 13 | 13 | 18 | 21 | 15 | 14 | 15 | 20 | 5 | 4 | 3 | 10 | 3 | 4 | 0 | 5 | 0 | 0 | 1 | 0 |
Peroxisomes | 50 | 13 | 38 | 42 | 35 | 45 | 39 | 28 | 29 | 18 | 28 | 23 | 36 | 42 | 57 | 14 | 5 | 9 | 27 | 2 | 25 | 0 | 1 | 0 |
SpindlePole | 126 | 15 | 66 | 36 | 47 | 42 | 42 | 60 | 48 | 48 | 26 | 23 | 10 | 10 | 15 | 41 | 15 | 9 | 17 | 20 | 19 | 3 | 1 | 2 |
Vac/Vac Membrane | 7 | 2 | 3 | 1 | 0 | 1 | 2 | 1 | 2 | 1 | 0 | 1 | 2 | 2 | 2 | 7 | 0 | 0 | 2 | 5 | 4 | 1 | 1 | 1 |
Unique Cell Count | 372 | 145 | 310 | 265 | 290 | 310 | 333 | 427 | 331 | 381 | 251 | 299 | 108 | 125 | 158 | 172 | 45 | 56 | 467 | 224 | 348 | 131 | 232 | 219 |
Labelled Cell Count | 576 | 206 | 510 | 428 | 421 | 503 | 534 | 653 | 507 | 571 | 392 | 442 | 179 | 179 | 244 | 231 | 67 | 77 | 467 | 224 | 348 | 131 | 232 | 219 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.2 | 5.0 | 4.2 | 3.9 | 4.2 | 3.7 | 3.5 | 3.5 | 2.9 | 3.0 | 2.9 | 2.8 | 5.3 | 5.5 | 4.9 | 9.4 | 9.0 | 8.8 | 5.0 | 4.8 | 5.3 |
Std Deviation (1e-4) | 1.7 | 1.6 | 1.1 | 1.1 | 1.3 | 1.2 | 1.1 | 1.0 | 0.7 | 1.0 | 0.8 | 0.9 | 1.4 | 1.5 | 1.4 | 7.1 | 3.3 | 5.2 | 1.5 | 1.7 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.02 | -0.15 | -0.25 | -0.26 | -0.52 | -0.47 | -0.51 | -0.55 | 0.35 | 0.39 | 0.23 | 1.17 | 1.11 | 1.08 | 0.26 | 0.21 | 0.35 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 0.6 | 0 | -2.0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.4 | -1.7 | 0 | -4.7 | -2.4 | -2.8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.0 | -2.5 | 0 | -2.4 | -0.3 | -0.4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | -2.1 | 0 | -3.8 | -2.4 | -1.8 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.2 | 2.4 | 2.1 | 3.1 | 3.1 | 3.7 | 4.8 | 3.1 | 4.2 | 2.4 | 3.2 | 3.4 | 3.7 | 1.1 | 0.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.7 | -3.6 | 0 | -3.5 | -1.4 | -0.9 |
Nucleus | -2.6 | -2.5 | -2.9 | -2.2 | -1.4 | -3.4 | -1.7 | -1.7 | -3.0 | -2.3 | -3.0 | -3.5 | -1.3 | 0 | -1.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.9 | 5.2 | 0 | -1.4 | -0.2 | 0.8 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.8 | -3.3 | 0 | 0.6 | 1.8 | -0.9 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.4565 | 2.8943 | 2.4139 | 2.9316 | 3.7067 | 4.1825 | 2.2999 | 1.1201 | 0.9505 | 2.02 | 2.4484 | 2.5467 | 4.136 | 5.1672 | 4.6326 | 5.8786 | 5.7793 | 6.1898 |
Actin | 0.0488 | 0.0341 | 0.0414 | 0.0011 | 0.0023 | 0.067 | 0.1012 | 0.0259 | 0.0455 | 0.0018 | 0.0192 | 0.0422 | 0.0554 | 0.0553 | 0.0491 | 0.0011 | 0.0461 | 0.1125 |
Bud | 0.003 | 0.0193 | 0.0085 | 0.0002 | 0.0004 | 0.0011 | 0.0166 | 0.033 | 0.0183 | 0.0021 | 0.0017 | 0.0045 | 0.0026 | 0.0081 | 0.0042 | 0.0004 | 0.0037 | 0.0018 |
Bud Neck | 0.46 | 0.0455 | 0.5012 | 0.968 | 0.9898 | 0.9121 | 0.2541 | 0.038 | 0.5142 | 0.9321 | 0.9138 | 0.8592 | 0.2838 | 0.0979 | 0.4509 | 0.9218 | 0.8429 | 0.8302 |
Bud Periphery | 0.0039 | 0.0146 | 0.0087 | 0.0002 | 0.0007 | 0.0021 | 0.0312 | 0.0342 | 0.0233 | 0.0035 | 0.0032 | 0.0103 | 0.0033 | 0.0062 | 0.006 | 0.0005 | 0.0045 | 0.0044 |
Bud Site | 0.3569 | 0.7177 | 0.3636 | 0.0282 | 0.0032 | 0.0079 | 0.4137 | 0.738 | 0.3123 | 0.0206 | 0.0178 | 0.0306 | 0.4157 | 0.7439 | 0.3248 | 0.0093 | 0.0278 | 0.0055 |
Cell Periphery | 0.0029 | 0.0033 | 0.0028 | 0.0004 | 0.0007 | 0.0008 | 0.0055 | 0.0056 | 0.0037 | 0.0014 | 0.0012 | 0.0022 | 0.0022 | 0.0044 | 0.0024 | 0.0004 | 0.0008 | 0.0012 |
Cytoplasm | 0.002 | 0.0518 | 0.02 | 0.0001 | 0.0001 | 0.0004 | 0.0092 | 0.0335 | 0.0118 | 0.0005 | 0.0003 | 0.0058 | 0.0066 | 0.0169 | 0.0126 | 0.0002 | 0.0014 | 0.0014 |
Cytoplasmic Foci | 0.0847 | 0.0103 | 0.0136 | 0.0005 | 0.0007 | 0.0021 | 0.0282 | 0.0152 | 0.0206 | 0.0065 | 0.0016 | 0.0176 | 0.0464 | 0.0077 | 0.0347 | 0.0579 | 0.0187 | 0.0083 |
Eisosomes | 0.0005 | 0.0005 | 0.0005 | 0 | 0 | 0.0003 | 0.001 | 0.0002 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0004 | 0.0005 | 0.0008 | 0 | 0.0004 | 0.0003 |
Endoplasmic Reticulum | 0.0001 | 0.0015 | 0.0003 | 0 | 0 | 0 | 0.0054 | 0.0015 | 0.0011 | 0.0001 | 0.0039 | 0.0004 | 0.0018 | 0.0007 | 0.0004 | 0 | 0.0008 | 0.0001 |
Endosome | 0.0015 | 0.0007 | 0.0002 | 0 | 0.0001 | 0.0005 | 0.0243 | 0.0022 | 0.0043 | 0.0041 | 0.0046 | 0.0041 | 0.0402 | 0.0002 | 0.0049 | 0.0001 | 0.0071 | 0.0006 |
Golgi | 0.0032 | 0.0006 | 0.0005 | 0.0001 | 0.0002 | 0.0013 | 0.0058 | 0.0013 | 0.0025 | 0.0019 | 0.0025 | 0.0024 | 0.0351 | 0.0005 | 0.0106 | 0.0004 | 0.0047 | 0.0012 |
Lipid Particles | 0.0058 | 0.0012 | 0.0006 | 0 | 0.0001 | 0.0004 | 0.0072 | 0.003 | 0.0038 | 0.01 | 0.0009 | 0.0015 | 0.024 | 0.0004 | 0.0129 | 0.0013 | 0.0201 | 0.0052 |
Mitochondria | 0.0018 | 0.0075 | 0.0003 | 0 | 0 | 0.0004 | 0.0144 | 0.0044 | 0.008 | 0.0016 | 0.0033 | 0.0008 | 0.0106 | 0.0192 | 0.0285 | 0.0001 | 0.0072 | 0.0004 |
None | 0.0006 | 0.0567 | 0.0297 | 0 | 0 | 0.0001 | 0.0067 | 0.0226 | 0.004 | 0.0002 | 0.0002 | 0.0009 | 0.0057 | 0.0207 | 0.0207 | 0.0001 | 0.0007 | 0.0111 |
Nuclear Periphery | 0.0001 | 0.0013 | 0.0002 | 0 | 0 | 0 | 0.0347 | 0.0023 | 0.0012 | 0.0001 | 0.0111 | 0.0012 | 0.0035 | 0.0005 | 0.0002 | 0 | 0.0002 | 0.0001 |
Nucleolus | 0.0038 | 0.0075 | 0.0006 | 0.0005 | 0.0007 | 0.0011 | 0.004 | 0.0076 | 0.0032 | 0.0027 | 0.0071 | 0.0032 | 0.0213 | 0.0047 | 0.001 | 0.0006 | 0.001 | 0.0002 |
Nucleus | 0.0007 | 0.0132 | 0.0012 | 0.0002 | 0.0003 | 0.0006 | 0.0129 | 0.009 | 0.004 | 0.0012 | 0.0029 | 0.0014 | 0.017 | 0.002 | 0.0007 | 0.0003 | 0.0006 | 0.0002 |
Peroxisomes | 0.016 | 0.0026 | 0.0013 | 0 | 0.0001 | 0.0008 | 0.0062 | 0.0039 | 0.0049 | 0.0051 | 0.0012 | 0.0037 | 0.0135 | 0.0073 | 0.0274 | 0.0036 | 0.0043 | 0.0141 |
Punctate Nuclear | 0.003 | 0.0053 | 0.0044 | 0.0003 | 0.0003 | 0.0005 | 0.009 | 0.0091 | 0.0093 | 0.0036 | 0.0014 | 0.0046 | 0.0081 | 0.0021 | 0.006 | 0.0019 | 0.0034 | 0.0011 |
Vacuole | 0.0009 | 0.0043 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0059 | 0.0085 | 0.0031 | 0.001 | 0.0009 | 0.003 | 0.0019 | 0.0007 | 0.0011 | 0.0001 | 0.0026 | 0.0001 |
Vacuole Periphery | 0.0001 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0.0027 | 0.0008 | 0.0006 | 0.0001 | 0.001 | 0.0004 | 0.0008 | 0.0001 | 0.0002 | 0 | 0.0007 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.1763 | 48.1381 | 52.9068 | 43.7806 | 15.4785 | 9.6469 | 64.112 | 78.2525 | 56.2559 | 23.6324 |
Translational Efficiency | 1.1417 | 1.4356 | 1.1365 | 0.9259 | 1.083 | 0.8882 | 1.0421 | 0.9245 | 1.0133 | 0.878 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1625 | 1159 | 1323 | 1020 | 1941 | 1852 | 1645 | 190 | 3566 | 3011 | 2968 | 1210 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 699.78 | 699.86 | 978.46 | 895.06 | 704.11 | 784.60 | 942.70 | 1020.70 | 702.14 | 751.98 | 958.64 | 914.79 |
Standard Deviation | 110.08 | 117.18 | 125.04 | 137.46 | 109.53 | 108.17 | 139.03 | 171.35 | 109.80 | 119.09 | 134.16 | 150.43 |
Intensity Change Log 2 | — | 0.000165 | 0.483611 | 0.355083 | — | 0.156157 | 0.420998 | 0.535686 | — | 0.080508 | 0.452548 | 0.448487 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001304 | 0.003579 | 0.007078 | 0.003289 | 0.002329 | 0.004362 | 0.002020 | 0.011319 | 0.001862 | 0.004061 | 0.004274 | 0.004550 |
Bud Neck | 0.367150* | 0.253178* | 0.267128* | 0.220245* | 0.353188* | 0.359255* | 0.355946* | 0.176526 | 0.359550* | 0.318423* | 0.316355* | 0.213380* |
Bud Site | 0.464998* | 0.446752* | 0.464089* | 0.600628* | 0.448663* | 0.465702* | 0.506054* | 0.709322* | 0.456107* | 0.458408* | 0.487348* | 0.617696* |
Cell Periphery | 0.016781 | 0.008088 | 0.004478 | 0.002067 | 0.015102 | 0.009352 | 0.003313 | 0.000876 | 0.015867 | 0.008866 | 0.003832 | 0.001880 |
Cytoplasm | 0.051230 | 0.058950 | 0.092600 | 0.052176 | 0.055233 | 0.042211 | 0.042592 | 0.042179 | 0.053409 | 0.048654 | 0.064883 | 0.050606 |
Cytoplasmic Foci | 0.023582 | 0.037339 | 0.019681 | 0.007336 | 0.032076 | 0.032040 | 0.010029 | 0.009647 | 0.028205 | 0.034079 | 0.014332 | 0.007699 |
Eisosomes | 0.001266 | 0.000460 | 0.001046 | 0.000233 | 0.000885 | 0.000937 | 0.000945 | 0.000282 | 0.001059 | 0.000754 | 0.000990 | 0.000241 |
Endoplasmic Reticulum | 0.000574 | 0.001363 | 0.001823 | 0.000599 | 0.000534 | 0.000235 | 0.000553 | 0.000307 | 0.000552 | 0.000669 | 0.001119 | 0.000553 |
Endosome | 0.008594 | 0.030530 | 0.004552 | 0.012063 | 0.011055 | 0.011131 | 0.004000 | 0.001765 | 0.009934 | 0.018598 | 0.004246 | 0.010446 |
Golgi | 0.002336 | 0.006957 | 0.001030 | 0.003279 | 0.002970 | 0.005593 | 0.001093 | 0.000552 | 0.002681 | 0.006118 | 0.001065 | 0.002851 |
Lipid Particles | 0.000770 | 0.003186 | 0.001698 | 0.000475 | 0.001316 | 0.001234 | 0.000423 | 0.000232 | 0.001067 | 0.001986 | 0.000991 | 0.000436 |
Mitochondria | 0.015592 | 0.045254 | 0.001593 | 0.007882 | 0.017605 | 0.012819 | 0.002163 | 0.008430 | 0.016688 | 0.025304 | 0.001909 | 0.007968 |
Mitotic Spindle | 0.002620 | 0.010102 | 0.007281 | 0.015106 | 0.005363 | 0.006045 | 0.008526 | 0.008874 | 0.004113 | 0.007607 | 0.007971 | 0.014127 |
None | 0.002521 | 0.003619 | 0.000732 | 0.000898 | 0.003046 | 0.000646 | 0.000369 | 0.000073 | 0.002807 | 0.001790 | 0.000530 | 0.000768 |
Nuclear Periphery | 0.000294 | 0.000167 | 0.000310 | 0.000116 | 0.000052 | 0.000029 | 0.000107 | 0.000034 | 0.000162 | 0.000082 | 0.000198 | 0.000103 |
Nuclear Periphery Foci | 0.001199 | 0.002581 | 0.001896 | 0.000293 | 0.000587 | 0.000853 | 0.000960 | 0.000283 | 0.000866 | 0.001518 | 0.001377 | 0.000291 |
Nucleolus | 0.001794 | 0.006266 | 0.000904 | 0.000195 | 0.001816 | 0.000360 | 0.001475 | 0.000065 | 0.001806 | 0.002633 | 0.001220 | 0.000175 |
Nucleus | 0.006159 | 0.011083 | 0.045110 | 0.006223 | 0.008462 | 0.002235 | 0.019718 | 0.002133 | 0.007412 | 0.005640 | 0.031036 | 0.005581 |
Peroxisomes | 0.016501 | 0.039681 | 0.026463 | 0.009846 | 0.026425 | 0.034832 | 0.013775 | 0.006884 | 0.021903 | 0.036699 | 0.019430 | 0.009381 |
Vacuole | 0.013704 | 0.026026 | 0.049579 | 0.054432 | 0.012564 | 0.009430 | 0.025428 | 0.019467 | 0.013083 | 0.015818 | 0.036193 | 0.048942 |
Vacuole Periphery | 0.001030 | 0.004841 | 0.000931 | 0.002621 | 0.000728 | 0.000699 | 0.000513 | 0.000751 | 0.000865 | 0.002294 | 0.000699 | 0.002327 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.08 | -6.79 | -4.53 | 0.25 | 4.07 | -2.73 | 0.30 | -3.61 | -2.91 | -3.64 | -4.53 | -5.59 | -4.94 | -1.30 | -0.71 |
Bud Neck | 9.91 | 10.95 | 14.90 | 4.43 | 3.63 | -0.53 | 1.41 | 10.88 | 11.12 | 10.04 | 5.28 | 8.25 | 18.59 | 13.52 | 11.25 |
Bud Site | 1.63 | 3.02 | -7.57 | -8.37 | -9.75 | -1.64 | -3.75 | -9.52 | -8.78 | -7.80 | -0.17 | -0.66 | -11.50 | -11.09 | -10.71 |
Cell Periphery | 4.89 | 8.50 | 10.72 | 5.25 | 3.80 | 4.12 | 9.95 | 12.58 | 10.11 | 5.61 | 6.37 | 13.04 | 15.82 | 10.16 | 5.00 |
Cytoplasm | -1.55 | -8.92 | -3.07 | -1.49 | 5.44 | 3.29 | 0.35 | -0.11 | -1.47 | -0.27 | 1.38 | -6.49 | -2.84 | -3.75 | 2.09 |
Cytoplasmic Foci | -4.21 | 1.00 | 7.10 | 9.85 | 6.55 | -0.11 | 8.72 | 4.77 | 4.76 | -0.05 | -2.71 | 7.26 | 10.75 | 12.33 | 4.82 |
Eisosomes | 6.22 | 1.55 | 8.30 | 5.29 | 5.24 | -0.29 | -0.14 | 4.92 | 4.34 | 5.79 | 2.55 | 1.06 | 9.77 | 5.74 | 8.39 |
Endoplasmic Reticulum | -4.90 | -7.08 | -2.11 | 3.40 | 6.12 | 2.56 | -2.09 | -0.63 | -2.71 | 1.00 | -1.34 | -7.03 | -2.29 | -1.15 | 5.29 |
Endosome | -9.14 | 4.16 | -1.40 | 6.75 | -3.61 | -0.11 | 7.53 | 9.00 | 8.48 | 3.37 | -7.20 | 8.46 | 0.12 | 4.91 | -3.55 |
Golgi | -6.48 | 5.78 | -0.89 | 4.03 | -2.81 | -4.12 | 7.24 | 8.06 | 8.16 | 2.53 | -7.20 | 9.24 | 0.12 | 4.69 | -2.61 |
Lipid Particles | -4.15 | -2.94 | 0.87 | 4.48 | 3.39 | 0.13 | 1.84 | 2.23 | 2.16 | 1.11 | -2.38 | -0.52 | 1.99 | 4.24 | 2.97 |
Mitochondria | -6.82 | 7.03 | 3.18 | 8.96 | -3.20 | 1.91 | 7.87 | 1.85 | 0.98 | -1.05 | -3.73 | 10.54 | 4.37 | 7.23 | -3.14 |
Mitotic Spindle | -6.19 | -4.17 | -5.15 | -1.98 | -3.05 | -0.54 | -2.89 | -1.03 | -0.91 | -0.24 | -4.26 | -4.72 | -4.64 | -3.06 | -2.63 |
None | -1.22 | 2.83 | 2.18 | 3.42 | -0.21 | 3.48 | 3.79 | 4.34 | 4.57 | 5.74 | 1.92 | 4.73 | 3.44 | 2.23 | -0.39 |
Nuclear Periphery | 0.54 | -0.43 | 0.55 | 0.04 | 2.78 | 2.48 | -4.04 | -1.06 | -2.53 | 3.33 | 0.75 | -1.09 | 0.11 | -2.92 | 2.99 |
Nuclear Periphery Foci | -2.39 | -1.81 | 2.37 | 4.50 | 4.42 | -1.75 | -4.01 | 1.81 | 2.86 | 5.12 | -2.56 | -2.90 | 2.89 | 5.35 | 6.17 |
Nucleolus | -3.46 | 1.84 | 3.60 | 4.97 | 3.72 | 3.00 | -0.17 | 3.66 | 3.90 | 4.30 | -1.42 | 0.78 | 4.91 | 5.16 | 5.03 |
Nucleus | -3.10 | -13.57 | -3.68 | -0.50 | 11.60 | 4.87 | -6.93 | 0.20 | -2.04 | 5.48 | 1.67 | -14.70 | -2.80 | -4.05 | 11.92 |
Peroxisomes | -6.70 | -2.78 | 3.00 | 8.69 | 5.03 | -2.62 | 5.34 | 7.97 | 9.41 | 3.38 | -6.25 | 1.72 | 6.72 | 11.42 | 4.84 |
Vacuole | -5.46 | -13.55 | -11.98 | -9.63 | -2.04 | 1.97 | -8.56 | -3.41 | -3.66 | -1.17 | -2.44 | -15.73 | -12.53 | -11.95 | -4.76 |
Vacuole Periphery | -5.14 | 0.25 | -2.46 | 2.73 | -2.16 | 0.16 | 1.35 | 0.11 | 0.05 | -0.32 | -4.66 | 0.70 | -2.77 | 0.28 | -2.89 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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