Standard name
Human Ortholog
Description Anillin-like protein involved in bud-site selection; required for the axial budding pattern; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; functions as a platform linking the cytokinesis tag septins to the axial landmark through its multiple domains; in vivo substrate of Cdc28p/Clb2p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.06 0.06 0.06 0 0.06 0.06 0 0 0 0 0 0.14 0.08 0.11 0 0 0.07 0 0.05 0.05 0 0.06 0
Bud 0.11 0.17 0.22 0.17 0.19 0.19 0.22 0.2 0.19 0.21 0.2 0.2 0.11 0.14 0.11 0.05 0.07 0.05 0.27 0.16 0.2 0.16 0 0
Bud Neck 0.2 0.14 0.22 0.14 0.24 0.15 0.15 0.12 0.11 0.1 0.14 0.11 0.09 0.12 0.08 0.13 0.2 0.2 0.53 0.43 0.47 0.3 0.07 0
Bud Site 0.35 0.2 0.36 0.44 0.27 0.31 0.23 0.21 0.21 0.17 0.18 0.16 0.43 0.26 0.39 0.19 0.18 0.23 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.17 0.12 0.15 0.19 0.18 0.21 0.21 0.23 0.27 0.22 0.25 0.21 0.24 0.24 0.25 0.18 0.16 0 0 0 0.43 0.74 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0.07 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Mitochondria 0 0.24 0.17 0.28 0.12 0.33 0.35 0.4 0.44 0.45 0.45 0.47 0.1 0 0.1 0.06 0.09 0.12 0 0 0 0 0 0
Nucleus 0.12 0.09 0.11 0.05 0.06 0.05 0.06 0.08 0 0.08 0.07 0.05 0 0 0 0.08 0.07 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0.09 0 0 0 0 0.05 0.05 0.05 0 0.06 0.07 0.05 0 0 0.06 0.07 0.07 0 0 0 0 0 0
Peroxisomes 0.13 0.09 0.12 0.16 0.12 0.15 0.12 0.07 0.09 0.05 0.11 0.08 0.33 0.34 0.36 0.08 0.11 0.16 0.06 0 0.07 0 0 0
SpindlePole 0.34 0.1 0.21 0.14 0.16 0.14 0.13 0.14 0.15 0.13 0.1 0.08 0.09 0.08 0.09 0.24 0.33 0.16 0 0.09 0.06 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 23 9 20 16 11 20 20 19 4 7 5 1 15 10 17 4 1 4 10 11 16 2 13 3
Bud 41 25 67 46 55 58 74 85 62 79 50 59 12 18 17 9 3 3 128 36 69 21 7 7
Bud Neck 76 20 69 36 69 48 50 52 37 37 34 34 10 15 13 23 9 11 247 96 164 38 15 1
Bud Site 129 29 111 116 78 96 78 88 69 64 46 47 46 32 61 33 8 13 – – – – – –
Cell Periphery 3 1 0 0 0 0 1 1 1 0 0 0 1 2 0 2 0 0 0 0 0 0 0 0
Cytoplasm 35 24 37 41 55 56 70 88 76 102 54 75 23 30 38 43 8 9 9 7 10 55 170 185
Endoplasmic Reticulum 0 1 0 1 1 1 2 0 0 0 0 2 0 1 0 5 3 1 0 0 2 0 3 1
Endosome 8 1 0 0 2 0 0 0 0 0 0 0 4 5 2 9 5 4 7 8 6 0 2 1
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 0 1 0 1 1 0 1 3
Mitochondria 10 35 53 75 36 103 117 172 146 172 114 140 11 5 16 10 4 7 6 7 12 2 4 4
Nucleus 45 13 35 14 16 15 21 35 14 29 18 14 4 3 3 13 3 2 1 3 4 0 0 0
Nuclear Periphery 1 5 4 2 3 5 0 3 4 0 2 3 0 0 0 0 0 0 4 6 7 0 0 0
Nucleolus 21 13 7 2 13 13 18 21 15 14 15 20 5 4 3 10 3 4 0 5 0 0 1 0
Peroxisomes 50 13 38 42 35 45 39 28 29 18 28 23 36 42 57 14 5 9 27 2 25 0 1 0
SpindlePole 126 15 66 36 47 42 42 60 48 48 26 23 10 10 15 41 15 9 17 20 19 3 1 2
Vac/Vac Membrane 7 2 3 1 0 1 2 1 2 1 0 1 2 2 2 7 0 0 2 5 4 1 1 1
Unique Cell Count 372 145 310 265 290 310 333 427 331 381 251 299 108 125 158 172 45 56 467 224 348 131 232 219
Labelled Cell Count 576 206 510 428 421 503 534 653 507 571 392 442 179 179 244 231 67 77 467 224 348 131 232 219


Bud Neck

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 5.0 4.2 3.9 4.2 3.7 3.5 3.5 2.9 3.0 2.9 2.8 5.3 5.5 4.9 9.4 9.0 8.8 5.0 4.8 5.3
Std Deviation (1e-4) 1.7 1.6 1.1 1.1 1.3 1.2 1.1 1.0 0.7 1.0 0.8 0.9 1.4 1.5 1.4 7.1 3.3 5.2 1.5 1.7 1.8
Intensity Change (Log2) – – – -0.1 0.02 -0.15 -0.25 -0.26 -0.52 -0.47 -0.51 -0.55 0.35 0.39 0.23 1.17 1.11 1.08 0.26 0.21 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 2.4 0.6 0 -2.0 0 0
Bud 0 0 0 0 0 0 0 0 0 -2.4 -1.7 0 -4.7 -2.4 -2.8
Bud Neck 0 0 0 0 0 0 0 0 0 -3.0 -2.5 0 -2.4 -0.3 -0.4
Bud Site 0 0 0 0 0 0 0 0 0 1.3 -2.1 0 -3.8 -2.4 -1.8
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.2 2.4 2.1 3.1 3.1 3.7 4.8 3.1 4.2 2.4 3.2 3.4 3.7 1.1 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -1.7 -3.6 0 -3.5 -1.4 -0.9
Nucleus -2.6 -2.5 -2.9 -2.2 -1.4 -3.4 -1.7 -1.7 -3.0 -2.3 -3.0 -3.5 -1.3 0 -1.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 2.0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 4.9 5.2 0 -1.4 -0.2 0.8
SpindlePole 0 0 0 0 0 0 0 0 0 -2.8 -3.3 0 0.6 1.8 -0.9
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4565 2.8943 2.4139 2.9316 3.7067 4.1825 2.2999 1.1201 0.9505 2.02 2.4484 2.5467 4.136 5.1672 4.6326 5.8786 5.7793 6.1898
Actin 0.0488 0.0341 0.0414 0.0011 0.0023 0.067 0.1012 0.0259 0.0455 0.0018 0.0192 0.0422 0.0554 0.0553 0.0491 0.0011 0.0461 0.1125
Bud 0.003 0.0193 0.0085 0.0002 0.0004 0.0011 0.0166 0.033 0.0183 0.0021 0.0017 0.0045 0.0026 0.0081 0.0042 0.0004 0.0037 0.0018
Bud Neck 0.46 0.0455 0.5012 0.968 0.9898 0.9121 0.2541 0.038 0.5142 0.9321 0.9138 0.8592 0.2838 0.0979 0.4509 0.9218 0.8429 0.8302
Bud Periphery 0.0039 0.0146 0.0087 0.0002 0.0007 0.0021 0.0312 0.0342 0.0233 0.0035 0.0032 0.0103 0.0033 0.0062 0.006 0.0005 0.0045 0.0044
Bud Site 0.3569 0.7177 0.3636 0.0282 0.0032 0.0079 0.4137 0.738 0.3123 0.0206 0.0178 0.0306 0.4157 0.7439 0.3248 0.0093 0.0278 0.0055
Cell Periphery 0.0029 0.0033 0.0028 0.0004 0.0007 0.0008 0.0055 0.0056 0.0037 0.0014 0.0012 0.0022 0.0022 0.0044 0.0024 0.0004 0.0008 0.0012
Cytoplasm 0.002 0.0518 0.02 0.0001 0.0001 0.0004 0.0092 0.0335 0.0118 0.0005 0.0003 0.0058 0.0066 0.0169 0.0126 0.0002 0.0014 0.0014
Cytoplasmic Foci 0.0847 0.0103 0.0136 0.0005 0.0007 0.0021 0.0282 0.0152 0.0206 0.0065 0.0016 0.0176 0.0464 0.0077 0.0347 0.0579 0.0187 0.0083
Eisosomes 0.0005 0.0005 0.0005 0 0 0.0003 0.001 0.0002 0.0004 0 0.0002 0.0001 0.0004 0.0005 0.0008 0 0.0004 0.0003
Endoplasmic Reticulum 0.0001 0.0015 0.0003 0 0 0 0.0054 0.0015 0.0011 0.0001 0.0039 0.0004 0.0018 0.0007 0.0004 0 0.0008 0.0001
Endosome 0.0015 0.0007 0.0002 0 0.0001 0.0005 0.0243 0.0022 0.0043 0.0041 0.0046 0.0041 0.0402 0.0002 0.0049 0.0001 0.0071 0.0006
Golgi 0.0032 0.0006 0.0005 0.0001 0.0002 0.0013 0.0058 0.0013 0.0025 0.0019 0.0025 0.0024 0.0351 0.0005 0.0106 0.0004 0.0047 0.0012
Lipid Particles 0.0058 0.0012 0.0006 0 0.0001 0.0004 0.0072 0.003 0.0038 0.01 0.0009 0.0015 0.024 0.0004 0.0129 0.0013 0.0201 0.0052
Mitochondria 0.0018 0.0075 0.0003 0 0 0.0004 0.0144 0.0044 0.008 0.0016 0.0033 0.0008 0.0106 0.0192 0.0285 0.0001 0.0072 0.0004
None 0.0006 0.0567 0.0297 0 0 0.0001 0.0067 0.0226 0.004 0.0002 0.0002 0.0009 0.0057 0.0207 0.0207 0.0001 0.0007 0.0111
Nuclear Periphery 0.0001 0.0013 0.0002 0 0 0 0.0347 0.0023 0.0012 0.0001 0.0111 0.0012 0.0035 0.0005 0.0002 0 0.0002 0.0001
Nucleolus 0.0038 0.0075 0.0006 0.0005 0.0007 0.0011 0.004 0.0076 0.0032 0.0027 0.0071 0.0032 0.0213 0.0047 0.001 0.0006 0.001 0.0002
Nucleus 0.0007 0.0132 0.0012 0.0002 0.0003 0.0006 0.0129 0.009 0.004 0.0012 0.0029 0.0014 0.017 0.002 0.0007 0.0003 0.0006 0.0002
Peroxisomes 0.016 0.0026 0.0013 0 0.0001 0.0008 0.0062 0.0039 0.0049 0.0051 0.0012 0.0037 0.0135 0.0073 0.0274 0.0036 0.0043 0.0141
Punctate Nuclear 0.003 0.0053 0.0044 0.0003 0.0003 0.0005 0.009 0.0091 0.0093 0.0036 0.0014 0.0046 0.0081 0.0021 0.006 0.0019 0.0034 0.0011
Vacuole 0.0009 0.0043 0.0004 0.0001 0.0002 0.0003 0.0059 0.0085 0.0031 0.001 0.0009 0.003 0.0019 0.0007 0.0011 0.0001 0.0026 0.0001
Vacuole Periphery 0.0001 0.0005 0 0 0 0.0001 0.0027 0.0008 0.0006 0.0001 0.001 0.0004 0.0008 0.0001 0.0002 0 0.0007 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.1763 48.1381 52.9068 43.7806 15.4785 9.6469 64.112 78.2525 56.2559 23.6324
Translational Efficiency 1.1417 1.4356 1.1365 0.9259 1.083 0.8882 1.0421 0.9245 1.0133 0.878

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Anillin-like protein involved in bud-site selection; required for the axial budding pattern; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; functions as a platform linking the cytokinesis tag septins to the axial landmark through its multiple domains; in vivo substrate of Cdc28p/Clb2p
Localization
Cell Percentages bud neck (36%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Bud4

Bud4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available