ORF
Human Ortholog
Description Xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.96 0.98 0.98 0.93 0.87 0.77 0.72 0.66 0.63 0.64 0.61 0.95 0.99 0.98 0.99 0.98 0.97 0.85 0.92 0.86 0.72 0.72 0.73
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.11 0 0 0 0.1 0.13 0.05 0.25 0.27 0.24 0.25 0 0 0 0 0 0 0.05 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0.06
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.07 0.18 0.25 0.19 0.2 0.2 0.24 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 2 1
Bud 0 4 0 2 0 3 5 6 7 9 7 8 0 0 0 0 0 0 1 0 5 7 8 11
Bud Neck 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 2 1 8 6 4 5 4 5 8 0 1 0 3 7 4 0 0 0 0 0 0
Cytoplasm 165 128 368 310 340 488 488 506 375 294 290 281 107 373 397 222 243 211 157 126 318 205 228 261
Endoplasmic Reticulum 2 0 0 0 0 0 0 0 3 1 3 2 0 0 1 10 9 15 0 0 1 3 0 2
Endosome 1 0 1 0 0 3 1 1 0 0 1 2 0 0 2 1 1 0 1 2 3 1 1 3
Golgi 2 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 3 1 0 1 0 1
Mitochondria 9 15 1 12 12 55 82 38 143 126 108 116 2 1 0 4 7 4 8 2 17 1 2 5
Nucleus 0 0 2 0 5 4 11 19 22 17 16 17 2 3 5 1 2 0 0 0 1 22 30 21
Nuclear Periphery 0 0 0 0 0 0 1 0 4 8 2 8 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 4 2 0 1 0 4 0 4 0 0 0 0 0 0 0 0 24 22 22
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 2 2 0 1 0 1 0 0 0 0 1 0 0 0 5 0 4 5 5 6
Vac/Vac Membrane 0 0 3 2 12 39 115 177 109 91 91 109 0 3 1 2 4 7 3 2 2 3 4 4
Unique Cell Count 175 133 374 315 364 558 634 704 571 463 455 459 113 377 407 224 248 218 186 138 370 287 318 358
Labelled Cell Count 179 147 380 330 373 606 712 753 669 551 527 551 115 381 408 243 273 242 186 138 370 287 318 358


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 3.7 5.5 5.1 5.7 5.5 5.8 6.9 5.9 6.4 8.0 8.3 4.9 5.6 5.6 5.8 6.6 7.2 7.4 7.8 7.5
Std Deviation (1e-4) 0.7 3.1 1.7 1.9 1.8 1.6 1.9 2.1 2.0 2.5 13.4 11.0 1.6 1.7 1.6 1.7 1.4 1.7 4.2 4.5 4.8
Intensity Change (Log2) – – – -0.11 0.06 0.0 0.07 0.33 0.11 0.23 0.55 0.59 -0.16 0.02 0.02 0.08 0.26 0.39 0.43 0.5 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.7684 5.7559 4.7061 4.1099 4.3216 4.6885 4.1707 4.7823 4.2851 4.468 4.5341 4.2919 1.0624 1.3047 1.2823 1.72 0.9243 1.1148
Actin 0.0394 0.0131 0.0162 0.0371 0.0109 0.0136 0.1088 0.0068 0.0462 0.0386 0.0007 0.0132 0.0033 0.0002 0.0005 0.0006 0.0003 0.0017
Bud 0.0061 0.0025 0.0066 0.0017 0.0031 0.0042 0.0008 0.0177 0.0109 0.0006 0.0001 0.0019 0.0005 0.0001 0.0024 0.0002 0 0.0008
Bud Neck 0.0105 0.0003 0.0027 0.0002 0.0038 0.0032 0.0009 0.0003 0.0008 0.0003 0.0001 0.0005 0.0062 0.0001 0.0001 0.0003 0.0001 0.0056
Bud Periphery 0.0056 0.0049 0.0056 0.005 0.004 0.0032 0.0012 0.0032 0.0077 0.0004 0.0001 0.003 0.0009 0 0.0019 0.0001 0 0.001
Bud Site 0.0185 0.0029 0.0051 0.0015 0.0054 0.0014 0.0094 0.0179 0.0329 0.0022 0.0001 0.0004 0.0028 0.0004 0.0007 0.0001 0.0001 0.0039
Cell Periphery 0.0009 0.0006 0.0003 0.0001 0.0007 0.0002 0.0003 0.0008 0.0004 0.0001 0 0.0001 0.0046 0.0002 0.0001 0 0 0.0001
Cytoplasm 0.3494 0.5954 0.5913 0.5069 0.4537 0.5712 0.301 0.5011 0.4739 0.4976 0.4213 0.605 0.3605 0.5634 0.5508 0.5543 0.3936 0.652
Cytoplasmic Foci 0.0234 0.012 0.0085 0.0089 0.0143 0.013 0.0258 0.0045 0.0146 0.0045 0.0072 0.0065 0.0191 0.0027 0.004 0.0049 0.017 0.0242
Eisosomes 0.0007 0.0001 0.0001 0.0001 0.0003 0.0001 0.0007 0.0002 0.0002 0.0002 0 0 0.0009 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0051 0.0031 0.0023 0.001 0.0055 0.0026 0.0079 0.0018 0.0035 0.0022 0.0038 0.0012 0.0154 0.0018 0.0029 0.001 0.0007 0.0011
Endosome 0.0185 0.0025 0.005 0.0019 0.009 0.0046 0.0425 0.0105 0.0038 0.004 0.0047 0.0006 0.0054 0.0004 0.0012 0.0015 0.0333 0.0025
Golgi 0.0096 0.0051 0.0036 0.0033 0.0021 0.0023 0.026 0.0125 0.0042 0.0072 0.0002 0.0002 0.0019 0.0001 0.0001 0.0001 0.0073 0.001
Lipid Particles 0.0328 0.0007 0.0062 0.0074 0.0016 0.0018 0.031 0.0004 0.0016 0.001 0.0001 0.0001 0.0208 0.0001 0.0002 0.0001 0.0127 0.0005
Mitochondria 0.0359 0.0011 0.0018 0.0078 0.0038 0.001 0.01 0.0011 0.0021 0.0024 0.0003 0.0001 0.003 0.0001 0.001 0.0001 0.0003 0.0002
None 0.392 0.3415 0.3317 0.3676 0.4338 0.358 0.3761 0.4175 0.386 0.4338 0.5497 0.3654 0.5342 0.4244 0.4173 0.4331 0.5299 0.2999
Nuclear Periphery 0.0062 0.0011 0.0009 0.0004 0.0218 0.0014 0.0213 0.0005 0.0011 0.0009 0.0026 0.0002 0.0038 0.0004 0.004 0.0005 0.0003 0.0003
Nucleolus 0.0014 0.0001 0.0002 0.0005 0.0014 0.0004 0.0016 0.0001 0.0003 0.0002 0.0001 0 0.0002 0 0.001 0 0 0.0001
Nucleus 0.0036 0.0017 0.0027 0.0016 0.0086 0.0089 0.0031 0.0012 0.0016 0.0015 0.0032 0.0008 0.0037 0.005 0.0077 0.0021 0.0005 0.0011
Peroxisomes 0.0234 0.0091 0.0064 0.0455 0.0008 0.0018 0.0156 0.0002 0.0051 0.0004 0.0002 0.0003 0.0032 0 0.0001 0.0001 0.0022 0.0021
Punctate Nuclear 0.0119 0.0005 0.0018 0.0006 0.0115 0.0061 0.0104 0.0005 0.0019 0.0015 0.0048 0.0003 0.0056 0.0003 0.0006 0.0004 0.0005 0.0016
Vacuole 0.0042 0.0016 0.0008 0.0006 0.0023 0.0007 0.0028 0.0008 0.001 0.0003 0.0005 0.0002 0.0034 0.0004 0.0015 0.0005 0.0006 0.0003
Vacuole Periphery 0.0011 0.0002 0.0003 0.0002 0.0017 0.0002 0.0026 0.0003 0.0002 0.0002 0.0002 0 0.0007 0 0.0016 0.0001 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.892 12.7799 16.3801 17.7479 13.4804 31.1744 21.732 22.9925 27.1078 23.2853
Translational Efficiency 0.9682 1.3497 0.9638 1.342 1.4476 1.2414 0.9288 1.0729 1.0999 1.1161

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1301 1213 439 127 2206 1696 227 85 3507 2909 666 212

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 672.33 740.48 902.13 1104.09 674.29 760.48 933.39 1033.63 673.56 752.14 912.78 1075.84
Standard Deviation 85.55 110.97 135.40 330.48 81.00 92.12 136.18 240.26 82.73 100.89 136.48 299.61
Intensity Change Log 2 — 0.139291 0.424166 0.715616 — 0.173541 0.469111 0.616279 — 0.156543 0.446846 0.666730

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000151 0.000477 0.000321 0.000886 0.000082 0.000192 0.000310 0.000229 0.000107 0.000311 0.000317 0.000622
Bud Neck 0.021617 0.038030 0.002477 0.004112 0.012324 0.036335 0.002021 0.001840 0.015771 0.037041 0.002322 0.003201
Bud Site 0.005156 0.016479 0.007246 0.048744 0.004080 0.009488 0.005118 0.003660 0.004480 0.012403 0.006521 0.030668
Cell Periphery 0.000204 0.000138 0.000212 0.000312 0.000117 0.000159 0.000255 0.000307 0.000149 0.000151 0.000227 0.000310
Cytoplasm 0.452735* 0.409147* 0.376591* 0.510115* 0.549676* 0.424145* 0.337996* 0.477586* 0.513713* 0.417891* 0.363436* 0.497073*
Cytoplasmic Foci 0.289939* 0.249952* 0.026969 0.021445 0.165840 0.235682* 0.038435 0.068611 0.211877* 0.241633* 0.030878 0.040356
Eisosomes 0.000240 0.000129 0.000075 0.000081 0.000105 0.000168 0.000133 0.000152 0.000155 0.000152 0.000094 0.000110
Endoplasmic Reticulum 0.000885 0.001241 0.003252 0.003441 0.000845 0.000636 0.004109 0.002221 0.000860 0.000888 0.003544 0.002952
Endosome 0.017594 0.026892 0.004544 0.004016 0.005934 0.023018 0.008078 0.006140 0.010260 0.024634 0.005749 0.004868
Golgi 0.003267 0.006522 0.000313 0.000177 0.001449 0.003875 0.000139 0.000119 0.002123 0.004979 0.000253 0.000154
Lipid Particles 0.012123 0.005912 0.002951 0.002389 0.005972 0.008652 0.004843 0.003837 0.008254 0.007509 0.003595 0.002970
Mitochondria 0.008370 0.011871 0.000727 0.000252 0.003009 0.011261 0.000350 0.000132 0.004998 0.011515 0.000599 0.000204
Mitotic Spindle 0.000727 0.005465 0.002172 0.003097 0.000586 0.003573 0.003550 0.001081 0.000638 0.004362 0.002642 0.002288
None 0.003479 0.003115 0.004934 0.006106 0.003285 0.004974 0.004091 0.002141 0.003357 0.004199 0.004647 0.004516
Nuclear Periphery 0.000356 0.000464 0.001349 0.000841 0.000376 0.000511 0.001706 0.000824 0.000368 0.000491 0.001471 0.000834
Nuclear Periphery Foci 0.000346 0.000560 0.002532 0.004426 0.000245 0.000237 0.004457 0.003322 0.000283 0.000372 0.003188 0.003983
Nucleolus 0.001019 0.001988 0.000845 0.000380 0.000720 0.002028 0.000994 0.000419 0.000831 0.002011 0.000896 0.000396
Nucleus 0.121713 0.118869 0.475996* 0.124024 0.206494* 0.144984* 0.412165* 0.222200* 0.175043* 0.134094* 0.454240* 0.163387*
Peroxisomes 0.008992 0.019147 0.000707 0.000691 0.002706 0.014739 0.000604 0.000398 0.005038 0.016577 0.000672 0.000574
Vacuole 0.050051 0.081405 0.085540 0.264301* 0.035632 0.072858 0.170379 0.204598* 0.040981 0.076422 0.114457 0.240363*
Vacuole Periphery 0.001036 0.002197 0.000246 0.000162 0.000521 0.002484 0.000266 0.000183 0.000712 0.002364 0.000253 0.000171

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.29 -5.31 -3.21 -0.79 -1.13 -5.43 -8.62 -6.78 -3.92 1.18 -8.23 -8.87 -4.72 -2.22 -0.79
Bud Neck -5.14 10.66 9.09 12.05 -0.81 -10.98 10.45 12.35 17.03 2.20 -11.70 14.59 12.56 19.74 -0.19
Bud Site -9.31 -0.91 -1.85 -0.15 -1.50 -5.23 -0.76 -1.24 4.02 0.11 -9.95 -1.34 -2.01 -0.01 -1.42
Cell Periphery 1.97 -0.28 -0.29 -2.56 -0.08 -3.65 -7.13 -4.11 -2.38 0.88 -0.05 -4.25 -2.84 -3.20 0.29
Cytoplasm 4.16 8.18 5.33 3.53 0.64 14.84 15.41 5.80 1.56 -2.83 14.33 17.95 8.92 3.90 -1.13
Cytoplasmic Foci 4.73 41.10 42.00 34.06 2.17 -10.85 28.05 17.62 24.56 -1.33 -5.43 48.31 41.51 43.52 -0.05
Eisosomes 7.85 12.38 12.79 5.80 1.58 -7.78 -4.05 -0.07 4.51 2.60 0.45 7.47 8.83 8.26 2.52
Endoplasmic Reticulum -2.08 -9.84 -4.66 -3.70 3.95 1.43 -9.65 -4.71 -5.21 3.84 -0.36 -13.64 -6.65 -6.64 5.36
Endosome -3.81 8.82 10.54 13.01 3.66 -11.38 -1.77 2.00 11.77 3.11 -11.08 6.53 10.33 18.03 4.42
Golgi -3.45 6.44 6.83 8.09 0.67 -4.83 5.99 6.06 8.32 0.85 -5.93 8.27 9.07 11.51 0.77
Lipid Particles 6.63 10.10 10.95 6.76 0.88 -3.44 2.55 4.38 6.33 2.47 1.29 9.99 11.47 9.27 2.16
Mitochondria -1.40 4.87 5.17 7.01 1.96 -6.29 5.83 6.59 9.04 3.24 -5.38 6.73 7.44 11.38 2.64
Mitotic Spindle -4.63 -2.08 -2.28 -1.25 -1.72 -2.87 -1.47 -1.44 -0.81 -0.84 -4.99 -2.59 -2.70 -1.58 -1.89
None 0.49 -0.74 -0.91 -1.23 -0.54 -1.84 0.15 3.62 3.70 2.26 -1.31 -0.80 -0.10 0.87 0.38
Nuclear Periphery -2.15 -15.51 -9.42 -8.23 1.59 -2.60 -13.46 -6.12 -4.90 5.95 -3.35 -20.63 -11.17 -9.44 4.82
Nuclear Periphery Foci -3.31 -5.23 -7.82 -7.27 -1.09 0.15 -5.68 -7.66 -7.68 0.28 -2.44 -7.67 -10.99 -10.68 -0.79
Nucleolus -2.19 2.10 5.71 4.39 5.28 -5.96 -0.59 5.93 8.23 4.19 -5.39 1.11 8.48 8.52 6.72
Nucleus -0.01 -23.26 -0.46 -0.45 17.99 10.79 -9.30 0.49 -2.32 6.60 9.22 -22.21 1.68 -1.65 17.42
Peroxisomes -5.41 11.22 11.95 11.01 0.95 -9.74 9.15 10.24 11.89 3.50 -10.97 13.17 15.07 16.17 1.47
Vacuole -8.32 -9.40 -15.36 -14.07 -11.63 -12.09 -13.25 -9.93 -8.72 -2.50 -14.81 -15.96 -18.06 -16.28 -10.75
Vacuole Periphery -4.92 10.17 12.37 9.60 3.71 -4.73 7.31 10.77 5.66 3.51 -6.30 12.15 16.87 8.66 4.75
ORF
Human Ortholog
Description Xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
Localization
Cell Percentages cytoplasm (31%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YJR096W

YJR096W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yjr096w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available