Standard name
Human Ortholog
Description Protein of unknown function; contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 1.0 0.9 0.68 0.84 0.9 0.89 – 0.79 0.82 0.72 0.75 0.93 0.86 0.92 0.94 0.83 0.89 0.89 0.79 0.88 0.94 0.92 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.05 – 0.29 0.26 0.36 0.32 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0 0.08 0.4 0.21 0 0.12 – 0.08 0 0.05 0.05 0.07 0.22 0.2 0.21 0.35 0.23 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 0 0 0 0 2 0 – 2 2 4 4 1 1 0 0 0 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 1 – 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 – 1 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 2 – 1 2 3 1 0 0 0 0 1 1 0 0 0 0 0 0
Cytoplasm 303 20 65 186 272 253 511 – 305 359 310 281 123 333 315 220 223 204 293 52 89 112 291 273
Endoplasmic Reticulum 2 0 0 0 0 0 2 – 0 0 0 1 0 0 1 11 3 4 0 0 0 0 1 0
Endosome 0 0 0 10 0 0 4 – 1 0 3 4 0 3 0 0 2 0 2 0 1 0 0 1
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0
Mitochondria 2 0 0 4 5 36 28 – 110 115 155 119 0 1 0 0 0 1 0 0 2 0 0 0
Nucleus 2 0 2 8 8 6 14 – 14 11 7 4 3 2 2 5 5 4 17 6 3 3 10 7
Nuclear Periphery 0 0 0 0 0 1 0 – 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 2 – 1 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 28 0 6 109 69 8 69 – 29 19 21 18 9 84 68 48 93 54 4 4 1 1 4 7
Unique Cell Count 321 20 72 272 325 282 577 385 440 429 375 132 385 344 234 269 230 328 66 102 119 316 298
Labelled Cell Count 337 20 73 318 356 306 633 464 513 505 434 136 424 386 284 327 268 328 66 102 119 316 298


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 7.2 6.9 7.9 7.3 4.6 5.4 – 4.4 4.5 4.3 4.4 8.6 7.8 7.9 13.2 13.3 13.0 8.1 7.9 7.5
Std Deviation (1e-4) 1.1 0.7 0.9 1.2 1.5 1.4 1.5 – 1.7 0.9 0.7 1.0 1.3 1.4 1.6 2.1 2.0 2.1 1.3 1.4 1.1
Intensity Change (Log2) – – – 0.2 0.08 -0.58 -0.35 – -0.64 -0.63 -0.67 -0.64 0.32 0.17 0.2 0.93 0.94 0.91 0.22 0.2 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.7 -1.4 -0.1 -0.4 – -2.2 -1.8 -3.3 -2.9 0.7 -0.9 0.4 1.1 -1.5 -0.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 5.1 2.5 0 0.9 – -0.2 0 0 0 -0.4 2.6 2.3 2.4 4.4 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2471 4.352 3.776 3.2763 3.1808 3.6465 4.4728 6.424 5.9523 5.4737 5.4145 5.7645 5.8845 7.7737 6.7479 5.8919 6.4389 6.7595
Actin 0.0261 0.0002 0.0006 0.041 0.0085 0.0007 0.0197 0.0027 0.0093 0.0059 0.0038 0.0014 0.0095 0.0004 0.0014 0.0004 0.0002 0.0004
Bud 0.0003 0.0001 0.0002 0.0008 0.0001 0.0001 0.0013 0.0042 0.0013 0.0004 0.0002 0.0001 0.0002 0.0007 0.0001 0.0002 0.0001 0.0003
Bud Neck 0.0047 0.0003 0.0006 0.0013 0.001 0.0022 0.0033 0.0003 0.0013 0.0012 0.0011 0.0022 0.0006 0.0007 0.0005 0.0009 0.0008 0.0021
Bud Periphery 0.0004 0 0.0001 0.0007 0.0001 0 0.0011 0.0008 0.0005 0.0005 0.0002 0.0001 0.0001 0.0003 0 0.0001 0 0.0001
Bud Site 0.0014 0.0004 0.0003 0.0013 0.0003 0.0001 0.0094 0.0025 0.0027 0.0007 0.0002 0.0001 0.0009 0.0019 0.0003 0.0002 0.0001 0.0001
Cell Periphery 0.0002 0 0 0.0001 0 0 0.0004 0 0.0002 0.0001 0 0 0.0001 0 0 0.0001 0 0
Cytoplasm 0.7048 0.8825 0.8443 0.7165 0.7967 0.7525 0.6751 0.8563 0.7408 0.721 0.7474 0.6969 0.8459 0.8742 0.8756 0.8378 0.8434 0.8
Cytoplasmic Foci 0.0201 0.0043 0.0054 0.0123 0.0102 0.0029 0.0234 0.0015 0.0082 0.0041 0.0053 0.0061 0.0082 0.0022 0.002 0.0018 0.0018 0.0042
Eisosomes 0.0002 0 0 0.0005 0 0 0.0003 0.0001 0.0001 0.0001 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0111 0.0025 0.004 0.0084 0.0049 0.003 0.0073 0.0016 0.0021 0.0036 0.0033 0.0027 0.003 0.0021 0.0019 0.0034 0.0013 0.0027
Endosome 0.0291 0.001 0.0043 0.0153 0.016 0.0037 0.0362 0.0009 0.0048 0.0126 0.0195 0.0069 0.01 0.0009 0.0006 0.0031 0.0007 0.0021
Golgi 0.0049 0 0.0002 0.0022 0.0016 0.0001 0.0092 0.0001 0.0021 0.0058 0.0025 0.0011 0.0011 0.0001 0.0001 0.0001 0 0.0001
Lipid Particles 0.0073 0 0.0014 0.0023 0.002 0.0001 0.011 0.0002 0.002 0.0105 0.009 0.0028 0.0016 0 0 0 0 0
Mitochondria 0.0019 0.0001 0.0003 0.0023 0.0007 0.0005 0.0121 0.0038 0.0114 0.0089 0.0118 0.0004 0.0004 0.0003 0.0002 0.0003 0.0002 0.0002
None 0.0367 0.019 0.009 0.0282 0.006 0.0076 0.0164 0.002 0.0036 0.0028 0.0024 0.002 0.0105 0.0043 0.0072 0.0023 0.0031 0.0133
Nuclear Periphery 0.0295 0.0035 0.0081 0.0104 0.0257 0.0135 0.0174 0.0062 0.0081 0.0164 0.009 0.0104 0.0058 0.005 0.0042 0.007 0.0041 0.0055
Nucleolus 0.0006 0.0001 0.0001 0.0003 0.0005 0.0002 0.0017 0.0001 0.0003 0.0003 0.0002 0.0003 0.0019 0.0006 0 0.0001 0.0001 0.0001
Nucleus 0.1032 0.0815 0.1167 0.1426 0.1075 0.2058 0.1323 0.1129 0.1936 0.1945 0.1706 0.2502 0.0925 0.1019 0.1038 0.1379 0.1384 0.1635
Peroxisomes 0.0039 0 0.0003 0.0053 0.0011 0 0.0039 0.0005 0.0017 0.0044 0.008 0.0037 0.0016 0.0001 0.0001 0 0 0.0001
Punctate Nuclear 0.0091 0.0035 0.0028 0.0051 0.015 0.0046 0.0111 0.0019 0.0044 0.0037 0.0033 0.011 0.005 0.0032 0.0014 0.0023 0.0052 0.0044
Vacuole 0.003 0.0007 0.0011 0.0024 0.0012 0.0019 0.0058 0.0009 0.0011 0.0015 0.0017 0.0012 0.001 0.001 0.0004 0.0018 0.0003 0.0007
Vacuole Periphery 0.0014 0.0001 0.0002 0.0006 0.0006 0.0005 0.0016 0.0002 0.0004 0.001 0.0006 0.0003 0.0002 0.0001 0.0001 0.0003 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.6931 13.3037 2.8206 10.8872 13.7824 10.6242 11.0493 16.2121 7.7856 14.8069
Translational Efficiency 3.4579 2.3701 9.0603 1.5486 2.1169 2.8604 1.8357 1.2407 1.9934 2.3041

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2150 548 1775 60 1440 1667 883 1492 3590 2215 2658 1552

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 861.62 1033.87 1061.16 1178.22 837.06 976.95 1035.65 1098.07 851.77 991.03 1052.69 1101.17
Standard Deviation 106.37 204.49 126.34 161.47 109.31 125.18 148.78 185.63 108.23 150.81 134.75 185.40
Intensity Change Log 2 — 0.262931 0.300519 0.451485 — 0.222954 0.307134 0.391567 — 0.243370 0.303782 0.422270

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000178 0.000524 0.000196 0.001560 0.000136 0.000110 0.000333 0.000439 0.000161 0.000212 0.000242 0.000482
Bud Neck 0.003257 0.002111 0.004158 0.002216 0.001267 0.003089 0.001343 0.002684 0.002459 0.002847 0.003223 0.002666
Bud Site 0.003740 0.007831 0.007093 0.009878 0.002271 0.003090 0.003040 0.005007 0.003150 0.004263 0.005747 0.005195
Cell Periphery 0.000087 0.000135 0.000075 0.000451 0.000128 0.000040 0.000100 0.000094 0.000104 0.000063 0.000083 0.000108
Cytoplasm 0.635450* 0.653482* 0.477614* 0.446660* 0.658086* 0.673480* 0.455917* 0.536970* 0.644530* 0.668532* 0.470406* 0.533479*
Cytoplasmic Foci 0.070568 0.034741 0.002486 0.037915 0.071635 0.021809 0.002715 0.003656 0.070996 0.025008 0.002562 0.004980
Eisosomes 0.000030 0.000028 0.000011 0.000066 0.000035 0.000010 0.000014 0.000010 0.000032 0.000015 0.000012 0.000013
Endoplasmic Reticulum 0.001237 0.002074 0.001684 0.007409 0.001763 0.000606 0.002597 0.001081 0.001448 0.000969 0.001987 0.001325
Endosome 0.002388 0.003419 0.000642 0.048248 0.004135 0.001284 0.000675 0.001419 0.003089 0.001812 0.000653 0.003230
Golgi 0.000406 0.000908 0.000051 0.006123 0.000670 0.000644 0.000078 0.002268 0.000512 0.000710 0.000060 0.002417
Lipid Particles 0.001459 0.000824 0.000368 0.013874 0.001915 0.000357 0.000490 0.000362 0.001642 0.000472 0.000408 0.000884
Mitochondria 0.001508 0.003742 0.000995 0.001412 0.001068 0.001025 0.000365 0.001888 0.001332 0.001697 0.000786 0.001870
Mitotic Spindle 0.002322 0.003885 0.005332 0.004163 0.001398 0.005165 0.005815 0.014148 0.001951 0.004848 0.005492 0.013762
None 0.003651 0.004647 0.006590 0.003499 0.004153 0.006615 0.009489 0.011818 0.003852 0.006128 0.007553 0.011497
Nuclear Periphery 0.000320 0.000413 0.001583 0.000981 0.000394 0.000247 0.000793 0.000539 0.000350 0.000288 0.001321 0.000556
Nuclear Periphery Foci 0.001029 0.002992 0.000776 0.006978 0.002115 0.001041 0.002667 0.001348 0.001465 0.001524 0.001404 0.001566
Nucleolus 0.000776 0.000230 0.000165 0.000259 0.000602 0.000366 0.000135 0.000135 0.000706 0.000333 0.000155 0.000140
Nucleus 0.236521* 0.218400* 0.459614* 0.197909* 0.208021* 0.255365* 0.475514* 0.384077* 0.225089* 0.246220* 0.464896* 0.376880*
Peroxisomes 0.000555 0.000512 0.000089 0.028072 0.000988 0.000731 0.000049 0.000113 0.000729 0.000677 0.000075 0.001194
Vacuole 0.034225 0.058498 0.030367 0.182020 0.038731 0.024697 0.037792 0.030670 0.036032 0.033059 0.032834 0.036521
Vacuole Periphery 0.000293 0.000603 0.000108 0.000308 0.000489 0.000230 0.000079 0.001273 0.000372 0.000322 0.000098 0.001236

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.32 -0.68 -2.48 -0.28 -2.30 0.88 -10.14 -5.90 -6.35 -0.03 -1.12 -3.18 -4.76 -3.63 -2.74
Bud Neck 0.79 -1.91 0.69 -0.01 2.60 -3.36 -4.90 -6.14 -0.47 -3.31 -1.21 -2.96 -2.27 -0.66 0.88
Bud Site -2.78 -1.52 -2.47 0.04 -1.33 -1.32 -1.49 -3.72 -2.38 -1.70 -1.90 -2.03 -2.69 -0.83 -0.21
Cell Periphery -3.26 1.04 -2.10 -1.12 -2.23 12.82 0.71 2.46 -11.30 1.71 5.94 1.77 -1.12 -6.30 -2.72
Cytoplasm 2.14 29.20 9.60 8.45 2.78 -1.06 26.61 24.68 26.48 -5.36 -1.12 39.32 28.88 27.03 -3.79
Cytoplasmic Foci 9.58 27.89 12.10 4.64 -2.10 15.70 24.16 23.73 13.53 -2.52 20.50 36.87 35.54 16.78 -3.93
Eisosomes -0.74 9.19 -4.53 -2.82 -6.94 14.16 6.94 9.05 -5.50 1.92 8.23 11.93 8.21 -1.61 -1.75
Endoplasmic Reticulum -4.66 -8.29 -2.60 -1.56 -1.57 5.88 -5.52 1.63 -7.96 8.07 3.12 -9.25 -0.74 -3.86 8.40
Endosome -1.08 6.87 -1.09 -0.96 -1.35 7.04 8.78 8.45 2.12 0.67 4.53 11.11 5.86 1.87 -0.82
Golgi -1.00 5.61 -0.73 -0.29 -1.16 0.20 2.74 -0.12 -0.23 -1.36 -0.68 4.81 -0.49 -0.20 -1.49
Lipid Particles 2.84 6.96 -1.20 -1.46 -1.74 8.92 6.35 6.07 -6.73 -0.57 10.26 9.60 5.27 -4.49 -3.47
Mitochondria -0.84 0.99 1.96 1.55 2.71 0.13 1.38 0.18 0.04 -1.64 -0.23 1.42 0.83 0.90 -0.49
Mitotic Spindle -1.15 -2.37 -0.89 -0.11 0.46 -2.58 -2.24 -4.97 -2.54 -2.07 -2.50 -3.33 -4.77 -2.79 -2.45
None -1.40 -6.27 -0.54 -0.19 0.80 -3.79 -6.38 -10.12 -5.58 -3.87 -4.62 -8.77 -11.57 -6.81 -6.41
Nuclear Periphery -6.30 -23.89 -6.34 -5.39 1.06 4.44 -15.32 -13.61 -16.82 2.33 0.42 -27.16 -16.44 -16.35 10.69
Nuclear Periphery Foci -5.25 -0.96 -3.85 -1.40 -3.73 2.99 -2.96 2.12 -1.53 4.47 -0.97 -2.30 -1.63 -0.13 0.18
Nucleolus 2.02 2.16 2.14 0.88 -0.08 1.21 2.51 2.25 1.98 -2.78 2.02 2.95 2.85 2.09 -1.18
Nucleus 0.44 -35.84 0.72 0.50 12.13 -6.82 -29.80 -26.29 -20.04 8.03 -4.88 -46.82 -27.12 -21.28 11.07
Peroxisomes 0.69 6.49 -0.89 -0.91 -1.02 0.70 2.61 2.54 3.14 -1.59 0.41 4.40 2.42 2.00 -1.04
Vacuole -8.37 -6.72 -9.48 -8.01 -8.89 3.30 -7.32 -12.35 -14.46 -3.11 -2.94 -9.65 -15.54 -13.04 -9.02
Vacuole Periphery -0.98 4.84 2.42 1.48 -3.34 2.18 3.56 0.24 -0.95 -1.61 1.00 5.36 -0.23 -0.60 -1.57
Standard name
Human Ortholog
Description Protein of unknown function; contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4
Localization
Cell Percentages cytoplasm (95%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Jjj3

Jjj3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Jjj3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available