Standard name
Human Ortholog
Description Adenosine kinase; required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.98 0.98 0.98 0.95 0.9 0.88 0.88 0.89 0.88 0.83 1.0 1.0 0.99 0.99 0.99 1.0 0.98 0.95 0.95 0.96 0.96 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0.09 0.1 0.05 0.07 0.06 0.09 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 7 3 4 4 0 0 0 0 0 0 0 0 0 0 0 0
Bud 5 1 0 0 0 0 2 1 2 1 1 1 1 0 2 1 0 0 0 2 0 0 6 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 2 4 4 5 4 6 13 7 14 17 0 2 7 11 1 4 0 0 0 0 0 0
Cytoplasm 220 266 247 265 355 302 379 277 217 201 238 181 517 739 785 396 145 337 201 237 236 377 660 540
Endoplasmic Reticulum 0 3 4 2 8 10 37 33 13 16 17 19 1 0 2 5 8 11 0 0 2 1 3 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 2 0 0 1 3 0 0 0 0 1 1 0 0 0 0 0 0 0 1
Mitochondria 1 0 0 0 0 0 0 0 3 0 2 1 0 0 0 2 0 0 0 0 0 1 0 0
Nucleus 0 0 0 0 0 0 1 3 0 0 0 0 0 0 0 0 0 0 0 3 2 2 3 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 2 0 2 0 0 5 2 3 6 3 1 2 3 1 3 0 1 1 2 2 3
Unique Cell Count 221 269 252 270 363 319 420 315 246 227 271 218 519 740 789 401 147 338 206 249 250 394 685 563
Labelled Cell Count 227 272 254 273 367 321 423 320 261 234 279 229 522 744 799 419 155 355 206 249 250 394 685 563


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 79.4 93.5 74.3 70.1 63.9 56.6 51.9 50.6 46.4 46.2 42.4 42.7 95.1 99.4 96.1 111.4 115.0 114.3 89.0 91.3 93.5
Std Deviation (1e-4) 16.3 24.5 15.2 15.0 13.1 10.9 11.6 11.0 11.6 9.7 9.4 9.0 22.8 23.0 19.7 35.4 28.1 35.5 19.9 19.2 23.0
Intensity Change (Log2) – – – -0.09 -0.22 -0.39 -0.52 -0.56 -0.68 -0.69 -0.81 -0.8 0.36 0.42 0.37 0.58 0.63 0.62 0.26 0.3 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 2.9 0 2.9 3.8 0 0 0 1.7 0 0
Cytoplasm 0.1 -0.2 -2.1 -3.9 -4.5 -4.3 -4.2 -4.5 -5.7 2.2 3.3 2.2 0.7 0.5 2.0
Endoplasmic Reticulum 0 0 1.2 3.8 4.3 2.3 3.0 2.7 3.6 0 0 0 0 0 1.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 63.3611 76.6276 71.4148 70.3672 62.0132 72.667 61.7582 78.4709 71.3694 69.2856 49.0094 68.7874 69.2 86.665 82.11 76.2674 62.4308 74.9364
Actin 0.0005 0.0001 0.0032 0.0001 0.0003 0.0001 0.0034 0.0001 0.0008 0.0002 0.0183 0.0023 0.002 0.0001 0.0053 0.0007 0.0117 0.0063
Bud 0.0006 0 0.0002 0 0.0048 0.0001 0.0007 0 0.0005 0.0001 0.0008 0.0001 0.0011 0 0.0001 0.0002 0.0007 0.0005
Bud Neck 0.0003 0.0002 0.0003 0.0002 0.0004 0.0004 0.0034 0.0001 0.0002 0.0002 0.0006 0.0003 0.0003 0.0001 0.0002 0.0002 0.0005 0.0008
Bud Periphery 0.0003 0 0.0001 0 0.0028 0.0001 0.0006 0 0.0002 0 0.0008 0 0.0006 0 0.0001 0.0002 0.0006 0.0016
Bud Site 0.0004 0 0.0002 0 0.0006 0 0.0016 0 0.0001 0.0001 0.0013 0 0.0008 0 0.0002 0.0004 0.0012 0.0009
Cell Periphery 0.0002 0.0003 0.0004 0 0.0016 0.0003 0.0008 0 0.0001 0.0004 0.0006 0 0.0005 0 0.0001 0.0018 0.0002 0.0002
Cytoplasm 0.9012 0.9826 0.9434 0.9444 0.8916 0.9356 0.8709 0.9947 0.9424 0.941 0.7454 0.9331 0.8736 0.9914 0.9544 0.9014 0.8076 0.897
Cytoplasmic Foci 0.0091 0.0001 0.0049 0.0001 0.0012 0.0112 0.0111 0.0001 0.0043 0.0039 0.0321 0.0057 0.0105 0.0001 0.002 0.0054 0.0377 0.0038
Eisosomes 0.0001 0 0.0001 0 0 0.0001 0.0001 0 0 0.0001 0.0004 0 0.0002 0 0.0001 0.0021 0.0001 0.0003
Endoplasmic Reticulum 0.0013 0.0005 0.0008 0.0009 0.0022 0.0005 0.0049 0.0001 0.0011 0.0011 0.0042 0.0003 0.0028 0.0001 0.0007 0.0009 0.0018 0.0021
Endosome 0.0014 0 0.0011 0.0001 0.0013 0.0002 0.0103 0 0.0018 0.0006 0.0148 0.0014 0.0075 0 0.0029 0.0005 0.011 0.0117
Golgi 0.0002 0 0.0011 0 0.0001 0.0001 0.0035 0 0.0001 0.0001 0.0141 0.001 0.0013 0 0.0009 0.0002 0.0048 0.0031
Lipid Particles 0.0015 0.0001 0.0019 0 0.0019 0.0018 0.007 0 0.0003 0.0017 0.0402 0.0102 0.0057 0 0.0021 0.0044 0.016 0.005
Mitochondria 0.0013 0 0.0013 0 0.0025 0.0016 0.004 0 0.0032 0.0005 0.0277 0.0001 0.0041 0 0.0017 0.0053 0.0062 0.0052
None 0.0085 0.0001 0.0054 0.0002 0.0021 0.0027 0.0072 0.0001 0.0089 0.0088 0.0359 0.0003 0.0093 0.0001 0.0019 0.0127 0.0055 0.0031
Nuclear Periphery 0.011 0.0029 0.005 0.0124 0.0074 0.0054 0.0094 0.0008 0.0051 0.0092 0.0131 0.0025 0.0123 0.0012 0.0094 0.0054 0.0141 0.0245
Nucleolus 0.0018 0 0.0024 0 0.0052 0.0034 0.0024 0 0.0021 0.0017 0.0045 0 0.005 0 0.0002 0.0119 0.0086 0.0017
Nucleus 0.0521 0.0123 0.0233 0.0409 0.0373 0.0304 0.0421 0.0039 0.0227 0.0244 0.0156 0.0201 0.0448 0.0067 0.0147 0.0332 0.0335 0.0215
Peroxisomes 0.0003 0 0.0002 0 0.0001 0.0002 0.0015 0 0.0001 0.0001 0.0052 0.0201 0.0008 0 0.0007 0.0003 0.0049 0.0011
Punctate Nuclear 0.0032 0.0001 0.0018 0.0004 0.0005 0.001 0.006 0 0.0023 0.0011 0.0065 0.0015 0.009 0 0.001 0.0011 0.0264 0.0051
Vacuole 0.0036 0.0007 0.002 0.0003 0.0214 0.0035 0.007 0.0001 0.0018 0.0031 0.0118 0.0008 0.0054 0.0002 0.0006 0.0054 0.0045 0.0024
Vacuole Periphery 0.0011 0 0.0011 0.0001 0.0146 0.0012 0.0022 0 0.0017 0.0018 0.006 0.0002 0.0023 0 0.0006 0.0061 0.0025 0.0022

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 451.932 449.317 455.7593 423.1243 432.1204 352.5059 394.5913 374.0688 417.9991 372.2745
Translational Efficiency 1.5861 1.7219 1.6017 1.8106 1.6994 2.0053 1.7347 1.8886 1.5564 1.6869

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1514 1193 2956 2304 1892 1384 2283 1168 3406 2577 5239 3472

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3537.01 5319.12 6570.11 8706.51 4256.57 5102.82 7651.45 7920.80 3936.72 5202.95 7041.33 8442.19
Standard Deviation 1102.22 1351.15 1362.51 2007.84 1006.35 1068.36 1519.34 2129.46 1109.26 1212.34 1530.00 2082.91
Intensity Change Log 2 — 0.588657 0.893387 1.299564 — 0.261603 0.846042 0.895955 — 0.419266 0.867722 1.093197

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000057 0.000092 0.000026 0.000128 0.000047 0.000045 0.000045 0.003523 0.000051 0.000066 0.000034 0.001270
Bud Neck 0.000269 0.000470 0.000340 0.001722 0.000248 0.000317 0.000350 0.005070 0.000257 0.000388 0.000345 0.002848
Bud Site 0.000526 0.000914 0.000540 0.004401 0.000306 0.000594 0.000888 0.016627 0.000404 0.000742 0.000692 0.008513
Cell Periphery 0.000386 0.000637 0.000122 0.000399 0.000286 0.000222 0.000559 0.001129 0.000330 0.000414 0.000312 0.000645
Cytoplasm 0.783606* 0.677775* 0.788779* 0.769562* 0.705509* 0.672110* 0.775468* 0.733278* 0.740224* 0.674733* 0.782979* 0.757356*
Cytoplasmic Foci 0.002457 0.004680 0.001471 0.003896 0.002170 0.002478 0.001563 0.015390 0.002297 0.003498 0.001511 0.007762
Eisosomes 0.000000 0.000001 0.000000 0.000002 0.000000 0.000000 0.000001 0.000013 0.000000 0.000001 0.000001 0.000005
Endoplasmic Reticulum 0.000042 0.000048 0.000009 0.000026 0.000023 0.000017 0.000015 0.000294 0.000031 0.000031 0.000012 0.000116
Endosome 0.000017 0.000055 0.000025 0.000191 0.000039 0.000042 0.000022 0.002960 0.000029 0.000048 0.000023 0.001123
Golgi 0.000341 0.000892 0.000067 0.000189 0.000858 0.000243 0.000073 0.002597 0.000628 0.000543 0.000069 0.000999
Lipid Particles 0.000174 0.000227 0.000082 0.000313 0.000256 0.000153 0.000162 0.001576 0.000219 0.000187 0.000117 0.000738
Mitochondria 0.000137 0.000164 0.000135 0.000334 0.000170 0.000115 0.000119 0.002296 0.000155 0.000138 0.000128 0.000994
Mitotic Spindle 0.000218 0.000113 0.000465 0.002001 0.000406 0.000347 0.000359 0.007068 0.000323 0.000239 0.000419 0.003705
None 0.017842 0.019642 0.011797 0.011873 0.014936 0.023935 0.012100 0.014124 0.016228 0.021948 0.011929 0.012630
Nuclear Periphery 0.000048 0.000097 0.000063 0.000132 0.000085 0.000091 0.000074 0.000317 0.000068 0.000094 0.000068 0.000194
Nuclear Periphery Foci 0.000609 0.000580 0.000163 0.000334 0.000863 0.000789 0.000247 0.001099 0.000750 0.000692 0.000199 0.000591
Nucleolus 0.000100 0.000108 0.000071 0.000202 0.000053 0.000064 0.000075 0.000748 0.000074 0.000084 0.000072 0.000386
Nucleus 0.190049* 0.290088* 0.191098* 0.190776* 0.271325* 0.296707* 0.201264* 0.169141* 0.235197* 0.293643* 0.195528* 0.183498*
Peroxisomes 0.000010 0.000005 0.000009 0.000025 0.000003 0.000003 0.000006 0.001242 0.000006 0.000004 0.000008 0.000434
Vacuole 0.002587 0.002935 0.004689 0.013373 0.001743 0.001660 0.006565 0.019646 0.002118 0.002250 0.005506 0.015483
Vacuole Periphery 0.000523 0.000477 0.000049 0.000122 0.000676 0.000068 0.000045 0.001864 0.000608 0.000257 0.000047 0.000708

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.05 2.64 -3.52 -0.37 -6.58 0.21 -0.42 -1.79 -1.79 -1.78 -1.89 2.14 -2.03 -1.89 -2.11
Bud Neck -3.30 -3.13 -8.32 -6.18 -7.35 -0.64 -5.64 -8.30 -7.98 -7.37 -2.76 -6.07 -11.78 -10.31 -10.32
Bud Site -1.89 0.12 -4.76 -3.80 -4.87 -1.29 -5.59 -7.01 -6.48 -6.19 -2.19 -4.47 -8.49 -7.35 -7.70
Cell Periphery -3.33 3.34 -0.96 2.74 -5.24 1.14 -0.89 -4.01 -4.39 -2.30 -2.46 0.71 -3.93 -1.82 -3.63
Cytoplasm 15.71 4.43 12.01 -5.51 9.13 4.93 -7.71 2.94 -1.19 9.30 13.87 -3.72 8.05 -5.73 12.63
Cytoplasmic Foci -4.99 0.41 -3.21 1.84 -3.96 -2.00 -1.97 -10.79 -9.86 -10.08 -5.02 -1.16 -10.35 -6.45 -10.08
Eisosomes -4.09 -2.89 -8.86 -4.76 -8.00 -0.64 -4.19 -4.97 -4.91 -4.63 -3.77 -5.07 -7.67 -6.75 -6.96
Endoplasmic Reticulum -0.19 2.22 1.16 1.04 -6.36 2.33 3.26 -1.37 -1.56 -1.65 0.12 3.13 -0.73 -0.75 -2.36
Endosome -1.60 -0.55 -2.34 -1.35 -2.19 -0.02 0.85 -2.08 -2.07 -2.13 -0.89 0.59 -2.51 -2.38 -2.58
Golgi -1.57 7.73 4.78 2.25 -1.50 1.55 1.98 0.36 -1.42 -1.98 0.39 2.51 1.32 1.17 -2.23
Lipid Particles -1.40 0.25 -6.57 -4.67 -7.54 1.20 0.34 -6.29 -7.73 -7.28 0.42 0.59 -7.77 -9.37 -10.08
Mitochondria -1.46 0.10 -2.31 -1.24 -2.15 3.70 2.91 -1.65 -1.87 -1.86 0.89 1.63 -2.14 -2.28 -2.45
Mitotic Spindle 0.75 -1.49 -2.46 -2.76 -1.50 0.43 0.27 -3.04 -3.29 -3.23 0.64 -0.64 -3.63 -4.09 -3.34
None -0.59 5.12 7.34 8.10 5.45 -5.44 4.75 6.77 10.71 2.59 -4.22 7.04 10.25 13.61 5.61
Nuclear Periphery -6.07 -9.11 -15.69 -9.06 -10.76 -0.10 -2.82 -11.70 -7.54 -10.39 -1.71 -6.24 -17.82 -9.46 -14.63
Nuclear Periphery Foci -0.08 4.86 0.23 0.37 -8.58 0.11 2.04 -2.04 -1.43 -9.69 0.11 3.41 -0.79 -0.65 -12.54
Nucleolus -0.31 0.39 -2.46 -2.68 -5.05 -0.82 -3.82 -2.91 -2.74 -2.51 -0.64 -0.76 -4.49 -4.23 -4.58
Nucleus -15.14 -4.77 -5.50 11.11 -1.19 -3.71 9.33 13.24 16.60 5.87 -12.49 4.67 6.07 18.65 2.15
Peroxisomes 2.12 0.75 -1.88 -4.50 -3.19 -0.96 -4.75 -1.34 -1.34 -1.32 1.47 -1.22 -1.43 -1.46 -1.41
Vacuole -0.98 -9.17 -22.29 -21.92 -18.84 -1.33 -14.57 -19.37 -19.21 -16.27 -1.66 -17.35 -29.27 -28.87 -24.15
Vacuole Periphery 0.13 1.56 1.41 2.01 -2.63 1.53 1.59 0.43 -1.59 -1.68 1.29 2.16 1.42 0.27 -2.00
Standard name
Human Ortholog
Description Adenosine kinase; required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Ado1

Ado1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ado1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available