Standard name
Human Ortholog
Description Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.69 0.69 0.87 0.93 0.93 0.95 0.95 0.93 0.94 0.94 0.95 0.93 0.93 0.91 0.99 0.83 0.81 0.69 0.07 0.23 0.18 0.27 0.23 0.19
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.05 0.06 0 0.13 0 0.17 0.05 0 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.29 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0
Vac/Vac Membrane 0.39 0.21 0.19 0.06 0.1 0.08 0.08 0.08 0.06 0.06 0.05 0.06 0 0.07 0 0.25 0.29 0.37 0.71 0.61 0.52 0.5 0.51 0.57
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 4 0 1 3 5 2 3 0 0 0 0 0 0 0 0 0 0 3 0 1 0
Bud 0 0 1 2 1 7 13 6 19 6 8 12 0 1 0 1 0 0 0 0 7 1 3 4
Bud Neck 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 2 5 2 6 1 5 4 2 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 34 36 311 299 434 611 805 792 493 582 633 728 25 188 287 186 140 169 7 20 66 45 68 48
Endoplasmic Reticulum 2 1 3 2 1 2 0 4 4 0 0 4 2 2 1 12 10 7 13 3 63 8 10 19
Endosome 0 0 6 8 9 3 13 4 2 4 5 7 0 5 2 3 10 8 4 2 11 6 13 9
Golgi 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 1 0 3 1 1 0
Mitochondria 1 15 0 1 1 0 3 4 15 7 20 24 0 1 0 2 5 6 1 3 14 5 6 3
Nucleus 0 1 0 0 1 0 4 2 1 1 1 2 0 1 0 5 2 6 0 0 1 0 2 3
Nuclear Periphery 1 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 2 1 2 0
Nucleolus 0 0 1 0 1 0 0 2 2 1 0 2 0 1 1 0 0 0 0 0 0 3 8 5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 0 1 0 3 3 0 1 0 0 0 0 0 0 0 7 15 9
Vac/Vac Membrane 19 11 68 19 47 51 64 67 33 40 31 49 1 15 8 56 50 91 74 52 194 85 148 147
Unique Cell Count 49 52 358 320 469 643 848 849 527 620 664 786 27 207 291 224 173 245 105 86 378 171 293 260
Labelled Cell Count 57 64 393 336 497 682 908 894 573 649 706 834 28 215 299 265 217 287 105 86 378 171 293 260


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 4.3 7.1 6.7 7.7 6.3 6.2 6.4 5.3 5.5 5.2 5.1 6.1 6.0 5.5 6.0 7.2 7.5 6.8 6.8 7.0
Std Deviation (1e-4) 1.3 0.9 1.9 1.8 1.8 1.3 1.3 1.3 1.6 1.3 1.1 1.0 1.0 1.4 1.1 1.3 1.6 1.7 1.9 1.9 1.9
Intensity Change (Log2) – – – -0.09 0.11 -0.18 -0.21 -0.16 -0.44 -0.37 -0.46 -0.5 -0.22 -0.26 -0.37 -0.25 0.01 0.07 -0.06 -0.08 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.7 2.6 5.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.3327 5.1926 4.811 4.7761 4.3831 5.0416 3.7393 4.4175 4.5159 4.1608 4.3573 4.7871 0.8225 1.6008 1.3134 2.0683 1.3445 1.4414
Actin 0.0434 0.0002 0.0026 0.0003 0.0665 0.0016 0.039 0.0001 0.0076 0 0.0008 0 0.0174 0.0001 0.0003 0.0074 0 0.0002
Bud 0.0008 0.0014 0.0011 0.0033 0.0003 0.0008 0.0009 0.0003 0.0005 0.0001 0.0002 0.0002 0.0003 0.0022 0.0003 0.0003 0.0008 0.0002
Bud Neck 0.002 0.0005 0.001 0.001 0.0006 0.0015 0.0013 0.0003 0.0148 0.0003 0.0007 0.0005 0.0008 0.0004 0.0005 0.0017 0.0002 0.0006
Bud Periphery 0.0014 0.0012 0.0017 0.0104 0.0003 0.0009 0.0019 0.0004 0.0007 0.0002 0.0002 0.0005 0.0003 0.0035 0.0005 0.0005 0.0009 0.0003
Bud Site 0.005 0.0047 0.0056 0.0098 0.0012 0.001 0.0014 0.0039 0.0055 0.0007 0.0006 0.0006 0.0058 0.0033 0.0024 0.0013 0.0005 0.0002
Cell Periphery 0.0016 0.0008 0.0008 0.0015 0.0002 0.0005 0.0007 0.0011 0.0009 0.0005 0.0002 0.0004 0.0006 0.0009 0.0029 0.0014 0.0004 0.0001
Cytoplasm 0.1761 0.3097 0.2844 0.2114 0.1465 0.3471 0.237 0.278 0.322 0.1871 0.1061 0.2281 0.3141 0.3905 0.4071 0.2571 0.4166 0.3919
Cytoplasmic Foci 0.0456 0.0283 0.0417 0.022 0.019 0.0428 0.0341 0.016 0.041 0.0255 0.0369 0.023 0.0578 0.0316 0.0415 0.0276 0.0333 0.0206
Eisosomes 0.0003 0.0001 0.0002 0 0.0001 0 0.0008 0 0.0001 0 0 0 0.0001 0 0 0.0005 0 0
Endoplasmic Reticulum 0.0356 0.0288 0.0331 0.0331 0.0601 0.0424 0.1225 0.1138 0.0696 0.0228 0.02 0.0726 0.086 0.082 0.0541 0.0593 0.0081 0.0351
Endosome 0.2238 0.1562 0.2098 0.2334 0.328 0.1833 0.1941 0.1703 0.2312 0.3106 0.3353 0.2129 0.1708 0.1134 0.2029 0.2058 0.2427 0.1831
Golgi 0.0127 0.0023 0.0043 0.0046 0.0043 0.0016 0.005 0.0034 0.0075 0.0028 0.0145 0.0041 0.0069 0.003 0.0041 0.0193 0.0014 0.0015
Lipid Particles 0.0238 0.0064 0.0088 0.0024 0.0021 0.0043 0.0119 0.0029 0.008 0.0063 0.0064 0.0022 0.0195 0.0051 0.0067 0.0227 0.001 0.0008
Mitochondria 0.0083 0.0021 0.0038 0.0015 0.0015 0.0024 0.0021 0.0022 0.0018 0.0009 0.0077 0.0046 0.0072 0.0007 0.0092 0.0296 0.0004 0.0262
None 0.1361 0.2025 0.1925 0.0967 0.0032 0.0904 0.1499 0.1019 0.0705 0.1019 0.166 0.0723 0.1072 0.0615 0.0757 0.0373 0.1051 0.0605
Nuclear Periphery 0.0218 0.0163 0.01 0.0243 0.0087 0.0162 0.0186 0.0389 0.0134 0.0089 0.0087 0.02 0.0254 0.032 0.0162 0.025 0.0027 0.0406
Nucleolus 0.0009 0.0011 0.0011 0.0006 0.0002 0.0006 0.0013 0.0003 0.0005 0.0003 0.0002 0.0002 0.0014 0.0005 0.0005 0.0008 0.0002 0.0002
Nucleus 0.0065 0.0076 0.0059 0.0044 0.0026 0.0083 0.0033 0.0103 0.0039 0.0027 0.0024 0.0047 0.0186 0.0087 0.0063 0.0078 0.0022 0.0063
Peroxisomes 0.021 0.0005 0.0038 0.0002 0.0067 0.0003 0.0031 0.0001 0.0039 0.0002 0.0133 0.0001 0.0129 0.0003 0.0028 0.0283 0.0001 0.0001
Punctate Nuclear 0.0044 0.0019 0.0045 0.0005 0.0003 0.001 0.0118 0.0004 0.0014 0.0005 0.0003 0.0003 0.0072 0.0008 0.0024 0.001 0.0004 0.0005
Vacuole 0.2118 0.2097 0.1573 0.2919 0.315 0.2257 0.1474 0.235 0.1771 0.2996 0.2448 0.3114 0.1309 0.2398 0.1481 0.2313 0.164 0.1931
Vacuole Periphery 0.0172 0.0178 0.026 0.0468 0.0326 0.0273 0.0118 0.0204 0.0182 0.0282 0.0343 0.041 0.0087 0.0199 0.0155 0.034 0.019 0.0378

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.153 19.9624 8.7135 12.9225 14.4764 14.4413 16.6221 14.0235 12.5488 15.9227
Translational Efficiency 0.4711 0.6286 0.9723 0.5846 0.658 1.3187 0.6474 0.7021 0.6804 0.7404

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1894 311 1739 1432 1787 1712 152 1602 3681 2023 1891 3034

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 785.11 939.61 917.03 909.87 833.98 853.28 914.29 922.18 808.83 866.55 916.81 916.37
Standard Deviation 99.42 131.42 117.41 131.72 119.71 106.72 108.91 132.24 112.42 115.17 116.75 132.14
Intensity Change Log 2 — 0.259167 0.224074 0.212766 — 0.033006 0.132639 0.145036 — 0.147104 0.177701 0.178276

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000053 0.000194 0.000250 0.002006 0.000108 0.000203 0.000208 0.000480 0.000080 0.000202 0.000247 0.001200
Bud Neck 0.006159 0.013543 0.005031 0.007465 0.004329 0.013876 0.004992 0.007951 0.005271 0.013825 0.005027 0.007722
Bud Site 0.003217 0.013433 0.013385 0.027593 0.006531 0.009389 0.013257 0.021381 0.004826 0.010010 0.013374 0.024313
Cell Periphery 0.000221 0.000205 0.000267 0.000212 0.000245 0.000150 0.000290 0.000143 0.000233 0.000158 0.000269 0.000175
Cytoplasm 0.142216* 0.180935* 0.488549* 0.345771* 0.217290* 0.272512* 0.254898* 0.479234* 0.178662* 0.258433* 0.469768* 0.416242*
Cytoplasmic Foci 0.075286 0.122319 0.027878 0.050283 0.095461 0.155620 0.082808 0.059299 0.085081 0.150500 0.032293 0.055043
Eisosomes 0.000091 0.000064 0.000035 0.000050 0.000062 0.000108 0.000144 0.000044 0.000077 0.000101 0.000044 0.000047
Endoplasmic Reticulum 0.050753 0.030410 0.010657 0.015509 0.133227* 0.027866 0.007309 0.014086 0.090792 0.028257 0.010388 0.014757
Endosome 0.060666 0.078952 0.011386 0.025384 0.057512 0.070235 0.033918 0.017392 0.059135 0.071575 0.013197 0.021164
Golgi 0.001692 0.007491 0.000276 0.004277 0.003553 0.004077 0.000223 0.001990 0.002595 0.004602 0.000272 0.003070
Lipid Particles 0.016406 0.007741 0.003724 0.007170 0.018554 0.018839 0.008710 0.006475 0.017449 0.017133 0.004125 0.006803
Mitochondria 0.001936 0.001412 0.000884 0.004481 0.001908 0.005253 0.000166 0.001672 0.001923 0.004662 0.000826 0.002998
Mitotic Spindle 0.000558 0.001356 0.005865 0.028250 0.000556 0.002643 0.002785 0.015494 0.000557 0.002445 0.005617 0.021515
None 0.000551 0.000124 0.003060 0.001821 0.000690 0.000409 0.002654 0.001262 0.000618 0.000365 0.003028 0.001526
Nuclear Periphery 0.002180 0.000844 0.008301 0.002509 0.002988 0.001795 0.003213 0.001747 0.002572 0.001649 0.007892 0.002107
Nuclear Periphery Foci 0.000957 0.002660 0.009617 0.007728 0.003292 0.001394 0.012885 0.008489 0.002091 0.001588 0.009880 0.008130
Nucleolus 0.000758 0.000356 0.000693 0.000459 0.000562 0.000625 0.001087 0.000508 0.000663 0.000584 0.000725 0.000485
Nucleus 0.025640 0.011895 0.210821* 0.050862 0.018049 0.032058 0.160163* 0.070652 0.021955 0.028959 0.206749* 0.061311
Peroxisomes 0.001108 0.003054 0.000427 0.001873 0.000773 0.002832 0.001182 0.001299 0.000946 0.002866 0.000488 0.001570
Vacuole 0.597741* 0.513248* 0.198246 0.409845* 0.420815* 0.373706* 0.408040* 0.287556* 0.511849* 0.395158* 0.215109* 0.345275*
Vacuole Periphery 0.011810 0.009765 0.000647 0.006454 0.013494 0.006410 0.001067 0.002846 0.012627 0.006926 0.000681 0.004549

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.16 -6.86 -2.85 -2.53 -2.38 -1.84 -2.85 -5.95 -3.23 -3.22 -2.90 -6.30 -3.60 -3.00 -2.74
Bud Neck -2.91 1.06 -1.33 2.56 -2.39 -8.66 -0.46 -3.14 5.14 -2.33 -8.63 -0.54 -3.23 6.32 -2.72
Bud Site -4.30 -5.18 -7.98 -2.19 -3.42 -2.14 -0.93 -5.52 -3.88 -0.77 -5.05 -4.27 -9.75 -5.30 -3.49
Cell Periphery 0.53 0.10 2.44 1.14 0.53 5.28 -0.96 8.65 2.01 4.17 4.95 0.17 6.67 1.12 0.89
Cytoplasm -3.13 -38.78 -23.25 -10.83 10.56 -6.63 -2.13 -24.86 -18.63 -9.59 -12.22 -34.50 -34.15 -18.21 3.71
Cytoplasmic Foci -5.44 18.00 10.68 9.48 -7.83 -12.56 2.14 12.08 24.01 3.37 -16.53 23.95 15.83 27.65 -9.56
Eisosomes 1.56 3.19 2.64 2.15 -3.72 -5.01 -5.63 3.24 8.75 7.50 -2.29 3.28 3.57 8.79 1.01
Endoplasmic Reticulum 3.24 14.38 13.33 3.43 -0.11 17.96 22.43 22.02 7.02 -1.28 16.96 25.77 25.16 8.07 -0.14
Endosome -2.94 27.54 20.82 10.58 -5.32 -4.27 5.02 23.25 23.32 3.47 -4.85 34.08 31.23 25.20 -3.15
Golgi -1.82 5.97 -0.94 1.59 -3.34 -0.68 7.59 4.09 4.26 -2.72 -2.74 9.34 2.00 3.72 -4.23
Lipid Particles 6.19 14.38 10.55 1.53 -4.59 -0.12 6.95 12.07 11.85 2.52 0.36 19.57 16.06 11.90 -4.86
Mitochondria 0.79 3.48 -0.75 -1.17 -2.15 -2.98 3.34 1.20 3.87 -2.31 -3.06 3.61 0.23 3.00 -2.27
Mitotic Spindle -1.09 -5.86 -7.42 -6.94 -4.43 -2.39 -0.85 -6.50 -5.01 -3.57 -2.54 -5.98 -9.84 -8.01 -4.81
None 1.67 -4.78 -2.14 -3.63 2.20 1.57 -1.38 -2.79 -7.05 1.02 1.58 -5.28 -3.14 -5.19 3.24
Nuclear Periphery 2.97 -9.27 -1.73 -4.28 7.99 2.65 -0.30 2.83 -0.22 1.68 2.82 -8.88 0.74 -2.36 9.40
Nuclear Periphery Foci -2.07 -12.57 -10.68 -3.71 3.74 6.52 -3.41 -6.36 -9.87 1.92 2.32 -11.36 -11.56 -12.43 3.62
Nucleolus 4.26 0.49 3.02 -2.34 2.50 -0.66 -3.01 0.62 1.56 3.55 1.12 -0.78 2.35 1.59 2.92
Nucleus 6.55 -37.00 -14.14 -17.62 26.90 -8.38 -9.05 -21.26 -15.85 5.05 -5.03 -39.04 -25.01 -20.10 27.26
Peroxisomes -3.07 5.89 -0.84 2.62 -4.12 -8.48 -2.37 -0.92 5.65 0.53 -8.27 6.67 -1.15 6.29 -4.29
Vacuole 4.79 41.73* 14.81 3.61 -22.24 4.97 0.44 8.92 4.34 3.17 14.86 34.96 17.19 1.09 -18.18
Vacuole Periphery 1.79 17.76 8.95 5.04 -4.50 9.23 17.29 14.70 8.72 -1.79 10.05 25.60 16.62 8.42 -5.34
Standard name
Human Ortholog
Description Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele
Localization
Cell Percentages ER (30%), vacuole (28%), mixed (33%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Tda4

Tda4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tda4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available