Standard name
Human Ortholog
Description JmjC domain family histone demethylase; promotes global demethylation of H3K4 and repression of noncoding intergenic transcription during sporulation; removes methyl groups added by Set1p methyltransferase; negatively regulated by H3K14 acetylation; protein levels regulated by Not4p polyubiquitin-mediated degradation; regulates sporulation timing by extending period of active transcription in opposition to programmed global transcriptional quiescence; regulates rDNA silencing
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Cytoplasm 0.06 0 0.07 0.14 0.09 0.12 0.09 0.11 0.09 0.07 0.11 0.09 0.07 0.18 0.19 – 0.06 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Mitochondria 0.05 0.31 0 0.07 0 0.33 0.34 0.17 0.67 0.61 0.72 0.46 0.13 0 0 – 0 – 0 0 0 0 0 0
Nucleus 0.89 0.85 0.9 0.78 0.87 0.72 0.74 0.76 0.65 0.63 0.6 0.69 0.85 0.76 0.73 – 0.88 – 0.8 0.8 0.75 0.64 0.46 0.41
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 – 0.19 – 0.1 0.1 0.17 0.22 0.35 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 1 1 0 4
Bud 0 1 0 1 1 3 6 2 13 9 14 7 1 0 0 – 0 – 2 1 2 2 8 10
Bud Neck 0 0 6 5 10 0 10 12 1 5 5 7 0 0 0 – 0 – 0 0 0 0 5 5
Bud Site 0 0 0 1 3 5 10 5 18 8 15 8 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Cytoplasm 9 6 32 46 26 52 49 58 37 29 41 34 23 71 70 – 1 – 2 5 9 4 15 10
Endoplasmic Reticulum 0 2 6 1 0 0 0 0 0 0 0 1 2 0 2 – 1 – 1 0 0 3 1 3
Endosome 0 0 1 0 0 0 0 0 0 0 0 1 0 4 3 – 0 – 1 2 3 1 3 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 1 0 0 0 3 0
Mitochondria 8 66 9 22 9 150 192 89 271 242 280 172 42 13 10 – 0 – 2 4 8 2 9 13
Nucleus 136 179 390 259 254 322 414 407 263 251 233 258 277 302 264 – 14 – 134 192 367 132 183 149
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 – 0 – 0 1 0 0 3 3
Nucleolus 0 0 0 0 0 0 0 2 1 1 3 0 1 0 0 – 0 – 0 0 0 2 7 7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 1
SpindlePole 0 0 8 6 2 4 13 13 3 8 5 9 0 1 1 – 0 – 2 3 8 2 10 14
Vac/Vac Membrane 7 7 0 13 5 17 12 17 4 10 4 12 11 25 32 – 3 – 16 24 81 46 141 122
Unique Cell Count 152 210 433 332 293 449 557 534 405 398 390 376 325 395 361 16 168 241 492 207 399 364
Labelled Cell Count 160 261 453 354 310 553 706 605 611 564 600 509 357 416 382 19 168 241 492 207 399 364


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.1 5.3 4.9 5.2 4.1 4.3 4.6 3.7 3.7 3.4 4.0 4.8 4.8 4.8 – 7.0 – 4.7 4.4 4.9
Std Deviation (1e-4) 1.1 0.6 0.7 1.3 0.9 0.8 0.8 0.9 0.7 0.9 0.7 0.6 1.0 1.2 1.5 – 0.9 – 1.5 1.3 1.9
Intensity Change (Log2) – – – -0.11 -0.03 -0.37 -0.31 -0.22 -0.52 -0.54 -0.66 -0.42 -0.14 -0.15 -0.13 – 0.4 – -0.18 -0.26 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4947 -0.3503 -0.5424 -0.6048 -0.9008 -0.3086 0.9924 0.8386 0.617 1.3307 2.5135 0.6866 0.6932 -0.3967 0.0094 -0.1057 -0.3603 -0.1837
Actin 0.0008 0.0001 0 0.0002 0.0002 0.0001 0.0236 0 0.0067 0.0013 0.0235 0.0007 0.0162 0 0.0124 0.0022 0.0001 0.0064
Bud 0.0001 0 0 0 0 0 0.0008 0.0001 0.0006 0.0003 0.0006 0.0001 0.0006 0 0.0001 0.0002 0 0.0001
Bud Neck 0.0018 0.0003 0.0002 0.0007 0.0004 0.0012 0.0029 0.0004 0.0017 0.0002 0.004 0.0011 0.0024 0.0004 0.0045 0.0007 0.0004 0.0014
Bud Periphery 0.0002 0 0 0 0 0 0.0018 0 0.0013 0.0013 0.0017 0.0003 0.0014 0 0.0002 0.0002 0 0.0004
Bud Site 0.0004 0.0004 0 0.0001 0 0.0001 0.0019 0.0009 0.0037 0.0005 0.0023 0.0003 0.0027 0.0003 0.0013 0.0001 0 0.0002
Cell Periphery 0.0001 0 0 0 0 0 0.0002 0 0.0001 0.0001 0.0045 0 0.0003 0 0.0001 0 0 0
Cytoplasm 0.017 0.0452 0.018 0.024 0.0031 0.0146 0.0154 0.0094 0.0085 0.0141 0.0224 0.0066 0.0168 0.0216 0.0103 0.0445 0.0062 0.0051
Cytoplasmic Foci 0.0053 0.0009 0.0008 0.0009 0.0003 0.0081 0.0028 0.0002 0.0003 0.0008 0.0056 0.0009 0.0166 0.0006 0.0057 0.0022 0.0001 0.0019
Eisosomes 0 0 0 0 0.0001 0 0.0002 0 0 0.0001 0.0004 0 0.0003 0 0.0001 0.0002 0 0.0001
Endoplasmic Reticulum 0.0028 0.0027 0.0007 0.0005 0.0002 0.0007 0.0033 0.0002 0.0005 0.0023 0.0106 0.0002 0.0046 0.0006 0.001 0.0017 0.0001 0.0004
Endosome 0.0068 0.0024 0.0009 0.0008 0.0004 0.0006 0.0114 0.0002 0.0007 0.0186 0.0259 0.0012 0.0279 0.0004 0.0034 0.0103 0.0003 0.0026
Golgi 0.0004 0 0 0 0 0.0001 0.0036 0 0.0004 0.0024 0.0128 0.0006 0.009 0 0.0016 0.0102 0 0.0019
Lipid Particles 0.0013 0.0002 0.0001 0.0002 0.0001 0.0019 0.0052 0 0.0001 0.003 0.0278 0.0047 0.0119 0 0.0061 0.0236 0 0.0038
Mitochondria 0.0017 0.0004 0.0002 0.0004 0.0003 0.0002 0.0107 0.0001 0.0003 0.0497 0.0316 0.0025 0.0084 0.0001 0.0008 0.0045 0.0002 0.0051
None 0.0363 0.0605 0.038 0.0996 0.037 0.0358 0.0137 0.0047 0.0086 0.01 0.0083 0.0123 0.0376 0.0382 0.0366 0.1077 0.0067 0.0136
Nuclear Periphery 0.0285 0.0255 0.0138 0.0201 0.0178 0.0062 0.0175 0.0074 0.0062 0.0194 0.0324 0.0044 0.0293 0.0085 0.0086 0.0149 0.0075 0.0065
Nucleolus 0.0115 0.0068 0.0044 0.0062 0.0085 0.0108 0.009 0.009 0.0056 0.0036 0.0125 0.01 0.0102 0.0056 0.0052 0.0042 0.008 0.0125
Nucleus 0.8572 0.8237 0.9017 0.7964 0.8605 0.8915 0.8601 0.9548 0.9375 0.8526 0.7117 0.9353 0.7666 0.9122 0.8769 0.7527 0.9465 0.9017
Peroxisomes 0.0002 0 0 0.0001 0 0.0019 0.0047 0 0.0001 0.0003 0.0092 0.0013 0.0045 0 0.0033 0.0013 0 0.0052
Punctate Nuclear 0.0244 0.0282 0.0203 0.0491 0.0707 0.0257 0.0085 0.0121 0.0162 0.0104 0.0185 0.0168 0.0259 0.0103 0.0213 0.0169 0.0236 0.0304
Vacuole 0.0023 0.0021 0.0006 0.0004 0.0001 0.0004 0.0018 0.0004 0.0008 0.0038 0.0194 0.0003 0.0044 0.0008 0.0004 0.0009 0.0001 0.0004
Vacuole Periphery 0.0009 0.0005 0.0001 0.0002 0.0001 0.0001 0.0011 0.0001 0.0001 0.0052 0.0144 0.0004 0.0026 0.0001 0.0002 0.0008 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.2976 6.3143 4.6854 3.7395 3.5978 7.0595 5.8143 6.1557 4.2575 6.4108
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
972 1099 167 1174 2018 1704 594 975 2990 2803 761 2149

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 629.45 729.04 1032.81 834.26 687.60 705.24 767.98 846.60 668.70 714.57 826.10 839.86
Standard Deviation 523.61 99.66 130.49 111.70 79.46 88.09 99.05 125.29 306.80 93.52 152.99 118.22
Intensity Change Log 2 — 0.211906 0.714411 0.406405 — 0.036545 0.159499 0.300111 — 0.123017 0.451320 0.351889

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000074 0.000168 0.001193 0.001206 0.000057 0.000278 0.000069 0.000717 0.000063 0.000235 0.000315 0.000984
Bud Neck 0.001612 0.004412 0.004699 0.017059 0.001388 0.004796 0.000842 0.011572 0.001461 0.004645 0.001689 0.014569
Bud Site 0.000624 0.001053 0.000991 0.018399 0.000288 0.002483 0.000239 0.015557 0.000397 0.001922 0.000404 0.017110
Cell Periphery 0.000285 0.000074 0.000069 0.000137 0.000089 0.000117 0.000277 0.000177 0.000153 0.000100 0.000231 0.000155
Cytoplasm 0.112377* 0.035149 0.001954 0.039912 0.039234 0.036538 0.035976 0.033033 0.063011 0.035993 0.028510 0.036791
Cytoplasmic Foci 0.017635 0.005148 0.000173 0.002555 0.004535 0.006986 0.004169 0.001566 0.008794 0.006265 0.003292 0.002106
Eisosomes 0.000030 0.000019 0.000050 0.000030 0.000012 0.000020 0.000053 0.000024 0.000018 0.000020 0.000052 0.000027
Endoplasmic Reticulum 0.000615 0.002273 0.010528 0.006947 0.001102 0.002075 0.000938 0.007257 0.000943 0.002153 0.003042 0.007088
Endosome 0.000588 0.000593 0.000359 0.002503 0.000266 0.000809 0.000189 0.001602 0.000371 0.000725 0.000226 0.002095
Golgi 0.000116 0.000556 0.000069 0.001600 0.000142 0.000357 0.000026 0.000871 0.000133 0.000435 0.000036 0.001269
Lipid Particles 0.002041 0.001161 0.000448 0.001742 0.000747 0.001733 0.000600 0.000466 0.001168 0.001509 0.000566 0.001163
Mitochondria 0.002255 0.002197 0.000828 0.003949 0.000950 0.003165 0.000225 0.002671 0.001374 0.002785 0.000358 0.003369
Mitotic Spindle 0.000568 0.001250 0.000054 0.026469 0.000452 0.001769 0.000226 0.020868 0.000489 0.001565 0.000189 0.023928
None 0.024554 0.006959 0.001144 0.006058 0.006226 0.007347 0.033241 0.006790 0.012184 0.007195 0.026197 0.006390
Nuclear Periphery 0.000605 0.000696 0.000547 0.002094 0.000646 0.000796 0.000321 0.002003 0.000633 0.000757 0.000371 0.002053
Nuclear Periphery Foci 0.000167 0.000264 0.000175 0.001195 0.000140 0.000344 0.000052 0.001057 0.000149 0.000313 0.000079 0.001132
Nucleolus 0.003984 0.003934 0.002274 0.001335 0.002079 0.003923 0.002678 0.002050 0.002698 0.003927 0.002589 0.001660
Nucleus 0.829111* 0.929839* 0.967909* 0.841244* 0.940081* 0.920779* 0.917947* 0.873706* 0.904006* 0.924331* 0.928911* 0.855972*
Peroxisomes 0.001118 0.002222 0.000254 0.001230 0.000723 0.003097 0.000114 0.000548 0.000852 0.002754 0.000145 0.000920
Vacuole 0.001511 0.001805 0.006158 0.021431 0.000776 0.002269 0.001774 0.014310 0.001015 0.002087 0.002736 0.018200
Vacuole Periphery 0.000132 0.000226 0.000123 0.002905 0.000066 0.000320 0.000044 0.003154 0.000088 0.000283 0.000061 0.003018

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.54 -12.87 -6.27 -5.71 1.40 -2.05 -2.13 -11.59 -5.52 -10.79 -2.63 -9.51 -10.02 -7.16 -6.38
Bud Neck -3.32 -4.81 -10.30 -7.72 -6.84 -4.79 1.39 -8.78 -5.63 -9.00 -5.88 -2.53 -13.63 -9.54 -12.81
Bud Site -1.22 -3.81 -6.06 -5.80 -5.71 -2.84 0.00 -4.92 -3.68 -4.92 -3.12 -1.42 -7.86 -6.50 -7.78
Cell Periphery 6.46 6.11 4.76 -4.59 -3.42 -1.81 -4.53 -2.32 -1.14 2.54 3.63 -2.21 0.97 -2.42 2.72
Cytoplasm 11.58 18.92 11.67 -0.62 -14.26 0.67 1.25 1.01 0.41 -0.31 8.34 9.83 8.15 -0.20 -3.14
Cytoplasmic Foci 5.36 9.11 8.39 3.46 -2.61 -2.69 0.77 4.27 6.06 3.68 1.49 6.14 9.04 6.92 3.21
Eisosomes 3.64 -5.52 -4.58 -8.94 2.52 -5.36 -6.44 -11.14 -6.97 1.38 -1.70 -7.19 -12.70 -11.21 1.65
Endoplasmic Reticulum -11.92 -12.35 -20.95 -16.57 2.74 -3.22 -1.12 -18.00 -13.46 -16.92 -6.25 -9.45 -27.24 -20.32 -13.51
Endosome 0.03 0.58 -3.31 -4.11 -4.81 -3.96 1.32 -5.77 -2.68 -6.20 -3.08 1.48 -6.81 -4.58 -8.19
Golgi -1.33 1.41 -1.68 -0.30 -1.81 -1.66 1.59 -1.61 -0.39 -2.34 -2.02 1.96 -2.18 -0.58 -2.73
Lipid Particles 2.11 3.74 1.67 -0.02 -1.29 -3.48 1.23 0.13 3.65 -1.26 -1.55 3.74 0.81 2.02 -1.63
Mitochondria 0.06 1.25 -0.78 -1.14 -5.04 -2.66 2.64 -3.59 0.89 -6.93 -2.10 2.56 -3.07 -0.02 -8.79
Mitotic Spindle -0.93 0.90 -6.76 -6.49 -6.96 -1.79 0.87 -6.10 -5.63 -6.17 -2.04 1.16 -9.14 -8.53 -9.27
None 6.67 9.94 7.82 1.01 -7.96 -1.03 -5.55 0.54 1.34 5.62 4.27 -3.45 6.21 1.79 5.36
Nuclear Periphery -1.48 -2.09 -16.34 -15.68 -14.29 -1.96 3.22 -9.20 -8.49 -9.82 -2.39 2.08 -17.41 -16.24 -17.65
Nuclear Periphery Foci -1.14 -1.33 -4.56 -4.12 -4.16 -1.53 3.17 -4.37 -3.47 -4.61 -1.84 2.01 -6.33 -5.10 -6.58
Nucleolus 0.17 2.08 0.09 -0.07 -2.08 -3.68 -1.51 -1.72 1.55 -0.68 -2.65 0.58 -1.39 0.79 -1.88
Nucleus -10.52 -14.88 -0.29 10.85 15.64 4.06 2.58 10.51 7.28 6.68 -3.63 -3.98 9.86 12.85 11.78
Peroxisomes -1.36 2.38 1.43 2.42 -1.59 -3.24 1.84 0.94 3.97 -1.80 -3.51 2.88 1.36 4.56 -2.70
Vacuole -1.32 -4.78 -11.94 -11.71 -7.71 -2.89 -4.60 -10.85 -9.94 -10.26 -3.31 -6.03 -16.06 -15.19 -14.23
Vacuole Periphery -1.67 -0.49 -2.29 -2.08 -2.27 -3.19 2.49 -1.80 -1.48 -1.83 -3.68 2.20 -2.83 -2.38 -2.87
Standard name
Human Ortholog
Description JmjC domain family histone demethylase; promotes global demethylation of H3K4 and repression of noncoding intergenic transcription during sporulation; removes methyl groups added by Set1p methyltransferase; negatively regulated by H3K14 acetylation; protein levels regulated by Not4p polyubiquitin-mediated degradation; regulates sporulation timing by extending period of active transcription in opposition to programmed global transcriptional quiescence; regulates rDNA silencing
Localization
Cell Percentages nucleus (92%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Jhd2

Jhd2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Jhd2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available