Standard name | |
---|---|
Human Ortholog | |
Description | JmjC domain family histone demethylase; promotes global demethylation of H3K4 and repression of noncoding intergenic transcription during sporulation; removes methyl groups added by Set1p methyltransferase; negatively regulated by H3K14 acetylation; protein levels regulated by Not4p polyubiquitin-mediated degradation; regulates sporulation timing by extending period of active transcription in opposition to programmed global transcriptional quiescence; regulates rDNA silencing |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 1 | 1 | 0 | 4 |
Bud | 0 | 1 | 0 | 1 | 1 | 3 | 6 | 2 | 13 | 9 | 14 | 7 | 1 | 0 | 0 | – | 0 | – | 2 | 1 | 2 | 2 | 8 | 10 |
Bud Neck | 0 | 0 | 6 | 5 | 10 | 0 | 10 | 12 | 1 | 5 | 5 | 7 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 5 | 5 |
Bud Site | 0 | 0 | 0 | 1 | 3 | 5 | 10 | 5 | 18 | 8 | 15 | 8 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 6 | 32 | 46 | 26 | 52 | 49 | 58 | 37 | 29 | 41 | 34 | 23 | 71 | 70 | – | 1 | – | 2 | 5 | 9 | 4 | 15 | 10 |
Endoplasmic Reticulum | 0 | 2 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | – | 1 | – | 1 | 0 | 0 | 3 | 1 | 3 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 3 | – | 0 | – | 1 | 2 | 3 | 1 | 3 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 1 | 0 | 0 | 0 | 3 | 0 |
Mitochondria | 8 | 66 | 9 | 22 | 9 | 150 | 192 | 89 | 271 | 242 | 280 | 172 | 42 | 13 | 10 | – | 0 | – | 2 | 4 | 8 | 2 | 9 | 13 |
Nucleus | 136 | 179 | 390 | 259 | 254 | 322 | 414 | 407 | 263 | 251 | 233 | 258 | 277 | 302 | 264 | – | 14 | – | 134 | 192 | 367 | 132 | 183 | 149 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 1 | 0 | 0 | 3 | 3 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 3 | 0 | 1 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 2 | 7 | 7 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 8 | 6 | 2 | 4 | 13 | 13 | 3 | 8 | 5 | 9 | 0 | 1 | 1 | – | 0 | – | 2 | 3 | 8 | 2 | 10 | 14 |
Vac/Vac Membrane | 7 | 7 | 0 | 13 | 5 | 17 | 12 | 17 | 4 | 10 | 4 | 12 | 11 | 25 | 32 | – | 3 | – | 16 | 24 | 81 | 46 | 141 | 122 |
Unique Cell Count | 152 | 210 | 433 | 332 | 293 | 449 | 557 | 534 | 405 | 398 | 390 | 376 | 325 | 395 | 361 | 16 | 168 | 241 | 492 | 207 | 399 | 364 | ||
Labelled Cell Count | 160 | 261 | 453 | 354 | 310 | 553 | 706 | 605 | 611 | 564 | 600 | 509 | 357 | 416 | 382 | 19 | 168 | 241 | 492 | 207 | 399 | 364 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 4.1 | 5.3 | 4.9 | 5.2 | 4.1 | 4.3 | 4.6 | 3.7 | 3.7 | 3.4 | 4.0 | 4.8 | 4.8 | 4.8 | – | 7.0 | – | 4.7 | 4.4 | 4.9 |
Std Deviation (1e-4) | 1.1 | 0.6 | 0.7 | 1.3 | 0.9 | 0.8 | 0.8 | 0.9 | 0.7 | 0.9 | 0.7 | 0.6 | 1.0 | 1.2 | 1.5 | – | 0.9 | – | 1.5 | 1.3 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.03 | -0.37 | -0.31 | -0.22 | -0.52 | -0.54 | -0.66 | -0.42 | -0.14 | -0.15 | -0.13 | – | 0.4 | – | -0.18 | -0.26 | -0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.4947 | -0.3503 | -0.5424 | -0.6048 | -0.9008 | -0.3086 | 0.9924 | 0.8386 | 0.617 | 1.3307 | 2.5135 | 0.6866 | 0.6932 | -0.3967 | 0.0094 | -0.1057 | -0.3603 | -0.1837 |
Actin | 0.0008 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0236 | 0 | 0.0067 | 0.0013 | 0.0235 | 0.0007 | 0.0162 | 0 | 0.0124 | 0.0022 | 0.0001 | 0.0064 |
Bud | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0.0001 | 0.0006 | 0.0003 | 0.0006 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 |
Bud Neck | 0.0018 | 0.0003 | 0.0002 | 0.0007 | 0.0004 | 0.0012 | 0.0029 | 0.0004 | 0.0017 | 0.0002 | 0.004 | 0.0011 | 0.0024 | 0.0004 | 0.0045 | 0.0007 | 0.0004 | 0.0014 |
Bud Periphery | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0018 | 0 | 0.0013 | 0.0013 | 0.0017 | 0.0003 | 0.0014 | 0 | 0.0002 | 0.0002 | 0 | 0.0004 |
Bud Site | 0.0004 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0.0019 | 0.0009 | 0.0037 | 0.0005 | 0.0023 | 0.0003 | 0.0027 | 0.0003 | 0.0013 | 0.0001 | 0 | 0.0002 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0045 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.017 | 0.0452 | 0.018 | 0.024 | 0.0031 | 0.0146 | 0.0154 | 0.0094 | 0.0085 | 0.0141 | 0.0224 | 0.0066 | 0.0168 | 0.0216 | 0.0103 | 0.0445 | 0.0062 | 0.0051 |
Cytoplasmic Foci | 0.0053 | 0.0009 | 0.0008 | 0.0009 | 0.0003 | 0.0081 | 0.0028 | 0.0002 | 0.0003 | 0.0008 | 0.0056 | 0.0009 | 0.0166 | 0.0006 | 0.0057 | 0.0022 | 0.0001 | 0.0019 |
Eisosomes | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0004 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0028 | 0.0027 | 0.0007 | 0.0005 | 0.0002 | 0.0007 | 0.0033 | 0.0002 | 0.0005 | 0.0023 | 0.0106 | 0.0002 | 0.0046 | 0.0006 | 0.001 | 0.0017 | 0.0001 | 0.0004 |
Endosome | 0.0068 | 0.0024 | 0.0009 | 0.0008 | 0.0004 | 0.0006 | 0.0114 | 0.0002 | 0.0007 | 0.0186 | 0.0259 | 0.0012 | 0.0279 | 0.0004 | 0.0034 | 0.0103 | 0.0003 | 0.0026 |
Golgi | 0.0004 | 0 | 0 | 0 | 0 | 0.0001 | 0.0036 | 0 | 0.0004 | 0.0024 | 0.0128 | 0.0006 | 0.009 | 0 | 0.0016 | 0.0102 | 0 | 0.0019 |
Lipid Particles | 0.0013 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0019 | 0.0052 | 0 | 0.0001 | 0.003 | 0.0278 | 0.0047 | 0.0119 | 0 | 0.0061 | 0.0236 | 0 | 0.0038 |
Mitochondria | 0.0017 | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0107 | 0.0001 | 0.0003 | 0.0497 | 0.0316 | 0.0025 | 0.0084 | 0.0001 | 0.0008 | 0.0045 | 0.0002 | 0.0051 |
None | 0.0363 | 0.0605 | 0.038 | 0.0996 | 0.037 | 0.0358 | 0.0137 | 0.0047 | 0.0086 | 0.01 | 0.0083 | 0.0123 | 0.0376 | 0.0382 | 0.0366 | 0.1077 | 0.0067 | 0.0136 |
Nuclear Periphery | 0.0285 | 0.0255 | 0.0138 | 0.0201 | 0.0178 | 0.0062 | 0.0175 | 0.0074 | 0.0062 | 0.0194 | 0.0324 | 0.0044 | 0.0293 | 0.0085 | 0.0086 | 0.0149 | 0.0075 | 0.0065 |
Nucleolus | 0.0115 | 0.0068 | 0.0044 | 0.0062 | 0.0085 | 0.0108 | 0.009 | 0.009 | 0.0056 | 0.0036 | 0.0125 | 0.01 | 0.0102 | 0.0056 | 0.0052 | 0.0042 | 0.008 | 0.0125 |
Nucleus | 0.8572 | 0.8237 | 0.9017 | 0.7964 | 0.8605 | 0.8915 | 0.8601 | 0.9548 | 0.9375 | 0.8526 | 0.7117 | 0.9353 | 0.7666 | 0.9122 | 0.8769 | 0.7527 | 0.9465 | 0.9017 |
Peroxisomes | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0019 | 0.0047 | 0 | 0.0001 | 0.0003 | 0.0092 | 0.0013 | 0.0045 | 0 | 0.0033 | 0.0013 | 0 | 0.0052 |
Punctate Nuclear | 0.0244 | 0.0282 | 0.0203 | 0.0491 | 0.0707 | 0.0257 | 0.0085 | 0.0121 | 0.0162 | 0.0104 | 0.0185 | 0.0168 | 0.0259 | 0.0103 | 0.0213 | 0.0169 | 0.0236 | 0.0304 |
Vacuole | 0.0023 | 0.0021 | 0.0006 | 0.0004 | 0.0001 | 0.0004 | 0.0018 | 0.0004 | 0.0008 | 0.0038 | 0.0194 | 0.0003 | 0.0044 | 0.0008 | 0.0004 | 0.0009 | 0.0001 | 0.0004 |
Vacuole Periphery | 0.0009 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0011 | 0.0001 | 0.0001 | 0.0052 | 0.0144 | 0.0004 | 0.0026 | 0.0001 | 0.0002 | 0.0008 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.2976 | 6.3143 | 4.6854 | 3.7395 | 3.5978 | 7.0595 | 5.8143 | 6.1557 | 4.2575 | 6.4108 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|