Standard name
Human Ortholog
Description Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.89 0.7 0.96 0.94 0.87 0.74 0.69 0.67 0.58 0.59 0.49 0.46 0.82 0.92 0.92 0.47 0.81 0.72 0.71 0.43 0.65 0.68 0.64 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.11 0 0.12 0.15 0.35 0.41 0.43 0.63 0.58 0.69 0.69 0 0 0 0.18 0 0.05 0 0.07 0.14 0.06 0.07 0
Nucleus 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.13 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Vac/Vac Membrane 0.12 0.18 0 0 0 0 0 0 0 0 0 0.07 0.17 0.09 0.07 0.29 0.13 0.15 0.1 0.17 0.09 0.1 0.14 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 7 4 1 2 11
Bud 0 2 0 2 0 6 2 6 10 8 10 4 0 0 5 0 0 0 1 10 2 7 7 13
Bud Neck 1 1 10 0 1 3 4 8 2 1 1 2 0 0 0 0 0 0 3 4 1 1 1 5
Bud Site 0 0 0 0 0 0 1 8 1 2 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 1 1
Cytoplasm 219 295 354 225 320 300 426 446 296 293 184 182 288 421 443 8 64 53 196 232 231 171 289 327
Endoplasmic Reticulum 1 4 5 0 2 0 0 1 0 0 0 0 0 3 1 2 5 3 1 1 5 3 3 7
Endosome 3 8 0 0 1 0 1 0 0 0 0 0 7 6 3 2 5 1 6 11 2 3 9 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 5 1 1 3 2
Mitochondria 2 44 9 29 56 142 249 290 318 290 261 273 4 1 16 3 0 4 12 40 47 16 31 18
Nucleus 11 24 2 1 4 3 14 22 9 17 14 8 8 6 3 0 3 4 10 69 2 8 17 7
Nuclear Periphery 0 1 0 0 0 0 0 1 0 1 1 0 0 0 1 0 0 0 1 1 1 1 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0
SpindlePole 1 1 1 0 3 2 7 4 5 1 4 0 0 0 1 0 2 2 6 30 4 3 15 13
Vac/Vac Membrane 29 77 0 3 9 11 24 19 15 14 11 28 58 40 36 5 10 11 27 90 31 25 61 51
Unique Cell Count 246 419 369 240 368 403 614 667 506 499 379 397 350 456 480 17 79 74 278 538 355 252 455 483
Labelled Cell Count 267 458 383 260 396 467 728 806 659 627 487 497 365 477 509 20 89 78 278 538 355 252 455 483


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 3.8 3.3 3.1 3.5 3.1 2.9 3.0 2.5 2.5 2.7 2.5 4.4 4.7 4.2 6.4 8.6 5.9 4.5 4.4 4.9
Std Deviation (1e-4) 1.2 0.9 0.7 0.8 1.6 1.1 0.9 0.9 0.7 1.1 1.0 1.0 1.3 1.7 1.8 2.3 14.3 2.2 1.7 1.6 1.7
Intensity Change (Log2) – – – -0.06 0.1 -0.08 -0.19 -0.12 -0.38 -0.36 -0.28 -0.39 0.42 0.51 0.37 0.96 1.4 0.85 0.47 0.44 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1304 1.9152 1.6445 1.3508 1.5096 1.9356 -0.1374 -1.5343 -1.221 -1.7972 -0.9088 -1.2132 4.3789 3.9591 3.237 4.6051 2.7137 3.2407
Actin 0.0001 0.0002 0.0003 0.001 0.0003 0.0004 0.0757 0.001 0.0042 0.0028 0.0109 0.0136 0.0421 0.0003 0.0006 0.0037 0.0005 0.0099
Bud 0 0.0002 0.0001 0.0002 0.0002 0.0001 0.0015 0.0041 0.0013 0.0019 0.005 0.0015 0.0121 0.0028 0.0021 0.0005 0 0.0005
Bud Neck 0.0003 0.0007 0.0012 0.0008 0.0009 0.0048 0.0069 0.0007 0.0038 0.0009 0.0066 0.004 0.0008 0.0001 0.0014 0.126 0.0001 0.0017
Bud Periphery 0 0.0002 0.0001 0.0002 0.0001 0.0002 0.002 0.0052 0.0013 0.0031 0.016 0.0036 0.0041 0.0032 0.0109 0.0004 0 0.0006
Bud Site 0.0002 0.0026 0.0009 0.0003 0.0002 0.0003 0.0087 0.0049 0.0062 0.0006 0.0112 0.0019 0.0386 0.0021 0.0138 0.0852 0 0.0007
Cell Periphery 0 0.0003 0.0001 0.0001 0.0001 0.0001 0.0004 0.0002 0.0002 0.0001 0.0008 0.0004 0.0004 0.0001 0.0009 0.0003 0 0.0001
Cytoplasm 0.1657 0.3603 0.2863 0.3227 0.3723 0.3503 0.1255 0.2103 0.1549 0.1819 0.1525 0.1528 0.1189 0.0648 0.0957 0.0398 0.1387 0.1632
Cytoplasmic Foci 0.0037 0.0054 0.0073 0.0111 0.0055 0.0087 0.0239 0.0175 0.0211 0.0156 0.009 0.0186 0.0096 0.0079 0.0041 0.2272 0.0094 0.0307
Eisosomes 0 0 0 0.0001 0 0 0.0007 0.0001 0.0001 0.0001 0.0002 0.0002 0.0003 0.0002 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0022 0.0061 0.0046 0.0049 0.0087 0.0048 0.0078 0.0061 0.0057 0.0072 0.0071 0.0067 0.0043 0.0012 0.0015 0.0002 0.0006 0.0019
Endosome 0.0024 0.0032 0.0058 0.0124 0.0074 0.0107 0.0178 0.0081 0.0146 0.0153 0.0122 0.0118 0.0295 0.0008 0.0016 0.0013 0.0002 0.0033
Golgi 0 0.0001 0.0001 0.0002 0.0001 0.0002 0.0057 0.0008 0.0014 0.0004 0.0018 0.0032 0.0045 0.0001 0.0003 0.0022 0.0001 0.0003
Lipid Particles 0.0001 0.0004 0.0004 0.0012 0.0005 0.0008 0.0097 0.0019 0.0039 0.002 0.0023 0.0049 0.0099 0.0011 0.0005 0.0016 0.0005 0.0009
Mitochondria 0.0003 0.0005 0.0006 0.0011 0.0008 0.0007 0.0056 0.0028 0.0018 0.0019 0.0035 0.016 0.0064 0.0005 0.0084 0.0002 0.0001 0.0005
None 0.1617 0.3676 0.3196 0.3427 0.4301 0.2747 0.1176 0.3638 0.2797 0.1439 0.1914 0.1328 0.5785 0.8454 0.7035 0.4656 0.7062 0.5994
Nuclear Periphery 0.0057 0.0068 0.0069 0.0112 0.0114 0.0086 0.0559 0.0197 0.0263 0.0339 0.0186 0.041 0.0356 0.0012 0.0034 0.0002 0.0014 0.0036
Nucleolus 0.0031 0.0021 0.0029 0.0021 0.0017 0.0036 0.004 0.0039 0.0085 0.0041 0.0114 0.0065 0.0031 0.0015 0.0023 0.0005 0.0002 0.0018
Nucleus 0.6467 0.2197 0.3463 0.2702 0.1455 0.3101 0.4442 0.3033 0.3801 0.5224 0.4992 0.5017 0.0731 0.0414 0.1414 0.0011 0.1394 0.1418
Peroxisomes 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0142 0.0004 0.0021 0.0008 0.001 0.0011 0.0056 0.0003 0.0015 0.006 0.0006 0.0011
Punctate Nuclear 0.0063 0.0187 0.0131 0.0098 0.0073 0.0172 0.0664 0.0411 0.0778 0.0483 0.03 0.0724 0.0081 0.0244 0.0031 0.0375 0.0017 0.0368
Vacuole 0.001 0.0043 0.003 0.007 0.006 0.0029 0.0037 0.0035 0.0038 0.0104 0.0077 0.0032 0.0084 0.0006 0.0026 0.0003 0.0001 0.0009
Vacuole Periphery 0.0001 0.0003 0.0003 0.0006 0.0007 0.0004 0.0023 0.0008 0.0011 0.0022 0.0016 0.0023 0.0061 0.0001 0.0003 0 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.3843 9.2852 9.1867 11.9158 13.2944 9.3164 11.9159 9.9921 10.9404 14.8249
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress
Localization
Cell Percentages nucleus (36%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rsf2

Rsf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rsf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available