Standard name | |
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Human Ortholog | |
Description | Cystathionine gamma-synthase, converts cysteine into cystathionine; STR2 has a paralog, YML082W, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 1 | 3 | 3 | 1 | 1 | 3 | 4 | 2 | 3 | 1 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 179 | 365 | 167 | 169 | 228 | 215 | 209 | 244 | 111 | 156 | 135 | 161 | 254 | 237 | 270 | 190 | 175 | 182 | 166 | 322 | 155 | 156 | 281 | 261 |
Endoplasmic Reticulum | 2 | 2 | 4 | 1 | 1 | 2 | 3 | 6 | 3 | 6 | 3 | 0 | 3 | 0 | 2 | 7 | 1 | 6 | 0 | 0 | 4 | 0 | 2 | 1 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Golgi | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 | 3 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 2 | 0 | 1 | 0 | 2 | 5 | 5 | 10 | 11 | 35 | 26 | 0 | 0 | 0 | 1 | 3 | 4 | 2 | 0 | 0 | 0 | 0 | 2 |
Nucleus | 1 | 3 | 9 | 6 | 5 | 6 | 11 | 21 | 11 | 20 | 9 | 15 | 2 | 2 | 1 | 3 | 0 | 4 | 5 | 35 | 11 | 6 | 11 | 18 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 4 | 10 | 12 | 20 | 45 | 45 | 61 | 104 | 27 | 54 | 26 | 73 | 12 | 22 | 20 | 11 | 27 | 22 | 2 | 7 | 11 | 2 | 4 | 7 |
Unique Cell Count | 181 | 375 | 181 | 182 | 261 | 256 | 280 | 362 | 149 | 230 | 190 | 251 | 261 | 242 | 283 | 198 | 190 | 207 | 182 | 378 | 189 | 166 | 308 | 300 |
Labelled Cell Count | 186 | 385 | 192 | 199 | 286 | 275 | 297 | 389 | 167 | 254 | 211 | 281 | 272 | 262 | 295 | 213 | 206 | 224 | 182 | 378 | 189 | 166 | 308 | 300 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.8 | 17.4 | 17.5 | 16.3 | 15.7 | 12.9 | 11.9 | 11.4 | 9.5 | 10.1 | 8.4 | 9.1 | 16.6 | 16.3 | 16.1 | 22.9 | 21.3 | 20.3 | 17.1 | 17.0 | 17.3 |
Std Deviation (1e-4) | 3.5 | 3.6 | 3.6 | 2.9 | 2.6 | 2.5 | 2.3 | 2.0 | 2.1 | 2.2 | 2.1 | 2.6 | 3.2 | 2.9 | 2.8 | 6.0 | 5.5 | 6.6 | 3.1 | 3.4 | 3.4 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.15 | -0.43 | -0.55 | -0.62 | -0.88 | -0.79 | -1.05 | -0.94 | -0.08 | -0.1 | -0.12 | 0.39 | 0.28 | 0.22 | -0.03 | -0.04 | -0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | -1.6 | -2.6 | -4.8 | -6.4 | -4.4 | -6.0 | -5.2 | -6.7 | 2.5 | 2.8 | 1.4 | 1.5 | -0.1 | -1.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.1 | 4.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.8 | -1.8 | -1.5 | -0.5 | 0.4 | 0.9 | 1.5 | -0.1 | 0.4 | 0 | 0 | 0 | -1.9 | 0 | -1.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.5 | 3.3 | 3.3 | 4.4 | 5.9 | 3.2 | 4.6 | 2.2 | 5.8 | -0.9 | 0.9 | 0.2 | -0.4 | 2.4 | 1.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.1021 | 12.236 | 10.1413 | 6.4773 | 7.1919 | 10.5139 | 7.2033 | 10.0009 | 9.1395 | 8.3561 | 7.3795 | 8.923 | 8.9311 | 12.0677 | 10.7868 | 10.7919 | 10.2386 | 10.3022 |
Actin | 0.0697 | 0.0009 | 0.0088 | 0.0231 | 0.0372 | 0.0016 | 0.0122 | 0.0014 | 0.0062 | 0.0033 | 0.0006 | 0.0013 | 0.0335 | 0.0026 | 0.0011 | 0.0101 | 0.0081 | 0.0005 |
Bud | 0.0008 | 0.0012 | 0.0007 | 0.0012 | 0.0011 | 0.0004 | 0.0006 | 0.0003 | 0.0006 | 0.0002 | 0.0004 | 0.0002 | 0.0009 | 0.0018 | 0.0005 | 0.0007 | 0.0016 | 0.0011 |
Bud Neck | 0.0028 | 0.0007 | 0.0037 | 0.0018 | 0.0017 | 0.0015 | 0.0009 | 0.0003 | 0.0011 | 0.0007 | 0.0008 | 0.0013 | 0.0016 | 0.0002 | 0.0009 | 0.0005 | 0.0017 | 0.0015 |
Bud Periphery | 0.0012 | 0.0012 | 0.0004 | 0.0008 | 0.0006 | 0.0007 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0007 | 0.0001 | 0.0013 | 0.0011 | 0.0004 |
Bud Site | 0.0062 | 0.0023 | 0.0049 | 0.0021 | 0.0016 | 0.0001 | 0.0021 | 0.0006 | 0.0017 | 0.0005 | 0.0004 | 0.0001 | 0.005 | 0.0011 | 0.0012 | 0.0008 | 0.0006 | 0.0001 |
Cell Periphery | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.6388 | 0.908 | 0.8008 | 0.8217 | 0.7281 | 0.7571 | 0.7732 | 0.9161 | 0.8236 | 0.6939 | 0.7014 | 0.8309 | 0.7534 | 0.9269 | 0.8591 | 0.6969 | 0.6414 | 0.8056 |
Cytoplasmic Foci | 0.0207 | 0.0038 | 0.0206 | 0.0109 | 0.019 | 0.0025 | 0.0257 | 0.0049 | 0.0142 | 0.0341 | 0.0139 | 0.0043 | 0.0194 | 0.0012 | 0.0038 | 0.0123 | 0.0158 | 0.0062 |
Eisosomes | 0.0005 | 0 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0085 | 0.0016 | 0.0023 | 0.0042 | 0.003 | 0.0039 | 0.0048 | 0.0026 | 0.0024 | 0.0054 | 0.0042 | 0.0026 | 0.0077 | 0.0009 | 0.0017 | 0.0056 | 0.003 | 0.0021 |
Endosome | 0.0516 | 0.0023 | 0.0102 | 0.015 | 0.0247 | 0.0107 | 0.0343 | 0.0013 | 0.0123 | 0.0655 | 0.0547 | 0.0071 | 0.0233 | 0.0004 | 0.0082 | 0.03 | 0.0433 | 0.0037 |
Golgi | 0.0189 | 0.0007 | 0.0081 | 0.0021 | 0.0434 | 0.0013 | 0.0061 | 0.0003 | 0.0087 | 0.0326 | 0.0016 | 0.0039 | 0.005 | 0.0001 | 0.0004 | 0.0095 | 0.0239 | 0.0003 |
Lipid Particles | 0.0123 | 0.0034 | 0.0037 | 0.0021 | 0.0105 | 0.0016 | 0.0091 | 0 | 0.0022 | 0.0063 | 0.0157 | 0.0066 | 0.0081 | 0.0001 | 0.0003 | 0.0053 | 0.0135 | 0.0002 |
Mitochondria | 0.0312 | 0.0018 | 0.0098 | 0.0117 | 0.0241 | 0.0074 | 0.0028 | 0.0005 | 0.0038 | 0.0042 | 0.002 | 0.0021 | 0.0056 | 0.0024 | 0.0009 | 0.0417 | 0.0046 | 0.0006 |
None | 0.0058 | 0.0083 | 0.002 | 0.0038 | 0.0018 | 0.0011 | 0.0061 | 0.0016 | 0.0018 | 0.0039 | 0.0208 | 0.0024 | 0.0063 | 0.0011 | 0.0017 | 0.0031 | 0.0015 | 0.0018 |
Nuclear Periphery | 0.0274 | 0.0056 | 0.0096 | 0.0088 | 0.0093 | 0.0235 | 0.0144 | 0.0034 | 0.0052 | 0.0047 | 0.0131 | 0.0073 | 0.0231 | 0.004 | 0.0072 | 0.011 | 0.0207 | 0.0099 |
Nucleolus | 0.0005 | 0.0001 | 0.0019 | 0.0004 | 0.0002 | 0.0003 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0009 | 0.0004 | 0.0006 | 0.0001 | 0.0004 | 0.0004 | 0.0002 | 0.0001 |
Nucleus | 0.0809 | 0.0544 | 0.1007 | 0.0785 | 0.0816 | 0.1792 | 0.0842 | 0.0598 | 0.1037 | 0.1379 | 0.1438 | 0.1222 | 0.0842 | 0.0548 | 0.1077 | 0.1547 | 0.2087 | 0.1613 |
Peroxisomes | 0.0084 | 0.001 | 0.0033 | 0.0021 | 0.0054 | 0.0002 | 0.0085 | 0.0053 | 0.0077 | 0.0023 | 0.002 | 0.001 | 0.0065 | 0.0001 | 0.0002 | 0.0039 | 0.0033 | 0.0002 |
Punctate Nuclear | 0.0023 | 0.0011 | 0.0058 | 0.0044 | 0.0043 | 0.0035 | 0.0064 | 0.0008 | 0.0021 | 0.0013 | 0.0086 | 0.005 | 0.0093 | 0.001 | 0.0032 | 0.0082 | 0.0041 | 0.0033 |
Vacuole | 0.0065 | 0.0013 | 0.0018 | 0.004 | 0.0015 | 0.0023 | 0.0058 | 0.0005 | 0.0015 | 0.0021 | 0.0127 | 0.0008 | 0.0036 | 0.0004 | 0.0012 | 0.0016 | 0.0015 | 0.001 |
Vacuole Periphery | 0.0045 | 0.0002 | 0.0006 | 0.0008 | 0.0007 | 0.0013 | 0.0018 | 0.0001 | 0.0007 | 0.0008 | 0.0023 | 0.0003 | 0.0016 | 0.0001 | 0.0003 | 0.002 | 0.0013 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.0457 | 7.7061 | 8.768 | 9.784 | 10.059 | 13.7849 | 18.3102 | 16.4776 | 16.0128 | 15.0017 |
Translational Efficiency | 0.8939 | 1.0586 | 0.802 | 0.825 | 0.5765 | 0.6209 | 0.5689 | 0.5463 | 0.5501 | 0.5641 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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