Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 16 | 14 | 15 | 16 | 6 | 4 | 7 | 4 | 2 | 5 | 1 | 3 | 5 | 4 | 0 | 19 | 9 | 26 | 10 | 11 | 7 | 4 | 11 | 10 |
Bud | 13 | 4 | 12 | 13 | 25 | 31 | 45 | 24 | 35 | 42 | 39 | 32 | 4 | 1 | 4 | 1 | 1 | 4 | 1 | 1 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 4 | 10 | 18 | 10 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 2 | 0 | 2 | 2 | 3 | 3 | 2 | 3 | 2 | 7 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 28 | 28 | 48 | 36 | 45 | 80 | 54 | 67 | 70 | 67 | 79 | 11 | 17 | 25 | 18 | 21 | 14 | 1 | 6 | 3 | 0 | 0 | 1 |
Endoplasmic Reticulum | 9 | 11 | 0 | 3 | 0 | 3 | 0 | 0 | 3 | 2 | 0 | 1 | 13 | 7 | 6 | 6 | 2 | 7 | 1 | 0 | 1 | 0 | 2 | 4 |
Endosome | 58 | 39 | 68 | 61 | 78 | 66 | 69 | 42 | 15 | 8 | 13 | 31 | 2 | 24 | 24 | 30 | 31 | 23 | 112 | 117 | 124 | 28 | 70 | 44 |
Golgi | 26 | 46 | 9 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 14 | 8 | 36 | 43 | 65 | 110 | 115 | 62 | 23 | 64 | 63 |
Mitochondria | 202 | 180 | 76 | 113 | 92 | 182 | 269 | 139 | 258 | 271 | 241 | 250 | 117 | 44 | 62 | 16 | 15 | 12 | 30 | 40 | 64 | 11 | 23 | 21 |
Nucleus | 0 | 1 | 0 | 2 | 2 | 1 | 1 | 4 | 3 | 3 | 4 | 5 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 8 | 1 | 0 | 0 | 1 | 2 | 1 | 9 | 0 | 4 | 4 | 2 | 2 | 0 | 2 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 17 | 8 | 20 | 18 | 25 | 33 | 39 | 19 | 9 | 1 | 7 | 11 | 2 | 3 | 5 | 0 | 0 | 0 | 53 | 20 | 40 | 4 | 23 | 21 |
SpindlePole | 20 | 12 | 73 | 40 | 58 | 64 | 99 | 69 | 35 | 41 | 33 | 68 | 2 | 2 | 3 | 9 | 11 | 17 | 10 | 8 | 13 | 4 | 1 | 1 |
Vac/Vac Membrane | 11 | 14 | 20 | 5 | 13 | 13 | 14 | 21 | 3 | 10 | 11 | 7 | 4 | 8 | 4 | 7 | 9 | 11 | 6 | 15 | 6 | 0 | 1 | 1 |
Unique Cell Count | 298 | 273 | 242 | 257 | 262 | 331 | 469 | 289 | 345 | 358 | 324 | 396 | 142 | 105 | 125 | 108 | 109 | 135 | 344 | 346 | 335 | 79 | 204 | 174 |
Labelled Cell Count | 390 | 359 | 323 | 325 | 341 | 448 | 633 | 394 | 443 | 479 | 434 | 511 | 172 | 127 | 146 | 146 | 143 | 183 | 344 | 346 | 335 | 79 | 204 | 174 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 6.4 | 6.2 | 6.1 | 6.5 | 5.0 | 5.0 | 5.6 | 4.5 | 4.2 | 4.2 | 4.7 | 5.8 | 6.2 | 6.1 | 10.0 | 10.4 | 9.5 | 5.6 | 5.7 | 6.5 |
Std Deviation (1e-4) | 0.7 | 1.1 | 1.3 | 1.8 | 1.6 | 1.4 | 1.0 | 1.6 | 1.8 | 1.2 | 1.6 | 1.0 | 0.8 | 1.1 | 0.9 | 1.7 | 2.0 | 1.7 | 0.8 | 1.3 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.05 | -0.32 | -0.33 | -0.17 | -0.48 | -0.57 | -0.57 | -0.41 | -0.11 | -0.01 | -0.03 | 0.67 | 0.74 | 0.61 | -0.15 | -0.13 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | -2.2 | -3.3 | -3.4 | -3.0 | 0 | 0 | 0 | 0 | -1.1 | -0.9 | -2.8 | 3.3 | 0.7 | 3.9 |
Bud | 0.1 | 2.0 | 2.0 | 2.2 | 1.5 | 0 | 0 | 0 | 0 | -1.0 | 0 | -0.8 | 0 | 0 | -0.9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.2 | 0.7 | 0.7 | 1.9 | 2.3 | 2.5 | 2.6 | 2.9 | 2.7 | -1.2 | 1.2 | 2.2 | 1.3 | 1.9 | -0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.1 | 0.4 | -2.3 | -4.3 | -3.8 | 0 | 0 | 0 | 0 | -6.5 | -1.0 | -1.9 | -0.1 | 0.1 | -2.4 |
Golgi | -1.5 | -2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.2 | 3.3 | 1.2 | 7.6 | 8.7 | 10.4 |
Mitochondria | 2.9 | 0.9 | 5.6 | 6.6 | 3.9 | 0 | 0 | 0 | 0 | 9.6 | 1.9 | 3.4 | -3.3 | -3.5 | -5.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -0.5 | 0.5 | 0.7 | 0 | -0.7 | 0 | 0 | 0 | 0 | -2.8 | -1.9 | -1.5 | -3.1 | -3.1 | -3.4 |
SpindlePole | -3.9 | -2.1 | -3.0 | -2.7 | -1.6 | 0 | 0 | 0 | 0 | -6.9 | -5.9 | -6.2 | -4.5 | -4.1 | -3.8 |
Vacuole | -3.2 | -1.5 | -2.2 | -3.1 | -0.4 | -4.5 | -3.0 | -2.5 | -4.0 | -2.1 | -0.2 | -1.9 | -0.6 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.9054 | 1.7417 | 1.3572 | 0.8529 | 0.9032 | 1.4995 | 1.0768 | 2.1609 | 1.7614 | 1.5498 | 1.6152 | 1.8527 | 1.208 | 1.9782 | 1.4216 | 1.7029 | 1.5282 | 1.4198 |
Actin | 0.031 | 0.0083 | 0.0094 | 0.0039 | 0.0071 | 0.0253 | 0.0105 | 0.0101 | 0.0257 | 0.0051 | 0.0445 | 0.0085 | 0.0081 | 0.0054 | 0.0057 | 0.006 | 0.0025 | 0.0042 |
Bud | 0.0003 | 0.0004 | 0.0001 | 0.0007 | 0.0011 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Bud Neck | 0.0014 | 0.0008 | 0.0004 | 0.0006 | 0.0009 | 0.0007 | 0.001 | 0.0003 | 0.0006 | 0.0004 | 0.0012 | 0.0005 | 0.0004 | 0.0015 | 0.0007 | 0.0008 | 0.0004 | 0.0004 |
Bud Periphery | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0.0007 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0023 | 0.0028 | 0.0005 | 0.0033 | 0.0023 | 0.0007 | 0.0019 | 0.0007 | 0.0006 | 0.0003 | 0.0026 | 0.0003 | 0.0005 | 0.0026 | 0.0012 | 0.0012 | 0.0006 | 0.0002 |
Cell Periphery | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.016 | 0.0137 | 0.0054 | 0.0028 | 0.0089 | 0.0025 | 0.0073 | 0.0062 | 0.0035 | 0.0035 | 0.002 | 0.0021 | 0.0026 | 0.0156 | 0.0041 | 0.0105 | 0.0034 | 0.0018 |
Cytoplasmic Foci | 0.2169 | 0.2305 | 0.2141 | 0.2313 | 0.2056 | 0.1722 | 0.2189 | 0.2638 | 0.2177 | 0.1691 | 0.1985 | 0.2024 | 0.2264 | 0.2323 | 0.2239 | 0.2126 | 0.2161 | 0.216 |
Eisosomes | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0016 | 0.0009 | 0.0006 | 0.0012 | 0.0014 | 0.0011 | 0.0018 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0004 | 0.0005 | 0.0004 | 0.0004 | 0.0004 | 0.0009 | 0.0003 |
Endosome | 0.3066 | 0.3319 | 0.3557 | 0.3982 | 0.2627 | 0.2564 | 0.3153 | 0.286 | 0.2967 | 0.3978 | 0.2949 | 0.2228 | 0.316 | 0.2398 | 0.3196 | 0.3367 | 0.2926 | 0.2266 |
Golgi | 0.2167 | 0.2918 | 0.2689 | 0.2426 | 0.3258 | 0.3496 | 0.3035 | 0.3254 | 0.3479 | 0.2976 | 0.3618 | 0.4297 | 0.2842 | 0.3019 | 0.3081 | 0.3175 | 0.2863 | 0.3612 |
Lipid Particles | 0.0835 | 0.0279 | 0.0277 | 0.0166 | 0.0721 | 0.031 | 0.0284 | 0.0042 | 0.0124 | 0.0164 | 0.0095 | 0.0104 | 0.0242 | 0.007 | 0.0158 | 0.0241 | 0.023 | 0.0206 |
Mitochondria | 0.0145 | 0.0145 | 0.0187 | 0.0216 | 0.0206 | 0.0163 | 0.0106 | 0.0049 | 0.0146 | 0.0051 | 0.0162 | 0.0108 | 0.0188 | 0.0051 | 0.0165 | 0.0098 | 0.0297 | 0.0109 |
None | 0.0047 | 0.0046 | 0.0011 | 0.0006 | 0.0002 | 0.0003 | 0.008 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0039 | 0.035 | 0.0026 | 0.0006 | 0.0002 | 0.0001 |
Nuclear Periphery | 0.0055 | 0.0002 | 0.0015 | 0.0013 | 0.0007 | 0.0014 | 0.005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 |
Nucleolus | 0.0004 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0049 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 |
Nucleus | 0.0046 | 0.0001 | 0.0004 | 0.0007 | 0.0005 | 0.0003 | 0.0022 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Peroxisomes | 0.0864 | 0.0627 | 0.0882 | 0.0686 | 0.0841 | 0.1368 | 0.0809 | 0.0936 | 0.0765 | 0.1003 | 0.0654 | 0.1088 | 0.1053 | 0.1485 | 0.0979 | 0.0757 | 0.14 | 0.1546 |
Punctate Nuclear | 0.0032 | 0.0038 | 0.0029 | 0.0007 | 0.0005 | 0.0009 | 0.0008 | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0008 | 0.0009 | 0.0003 | 0.0005 | 0.0012 | 0.0006 |
Vacuole | 0.0025 | 0.0032 | 0.0021 | 0.0023 | 0.0026 | 0.002 | 0.0019 | 0.0029 | 0.0013 | 0.0018 | 0.0006 | 0.0008 | 0.0015 | 0.0018 | 0.0015 | 0.0016 | 0.0012 | 0.0013 |
Vacuole Periphery | 0.0013 | 0.0013 | 0.0016 | 0.0019 | 0.0019 | 0.0015 | 0.0013 | 0.0007 | 0.001 | 0.0014 | 0.0008 | 0.0008 | 0.0008 | 0.0006 | 0.001 | 0.0011 | 0.0009 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.2213 | 17.647 | 15.5071 | 20.8623 | 21.2167 | 22.8451 | 20.4254 | 23.1348 | 23.656 | 23.9732 |
Translational Efficiency | 1.2839 | 0.8112 | 0.9254 | 0.787 | 0.7732 | 1.0067 | 0.6219 | 0.6585 | 0.7351 | 0.7266 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
309 | 1014 | 1189 | 542 | 1764 | 1058 | 1838 | 106 | 2073 | 2072 | 3027 | 648 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 716.22 | 814.41 | 1059.62 | 971.97 | 823.56 | 850.15 | 900.17 | 959.16 | 807.56 | 832.66 | 962.80 | 969.87 |
Standard Deviation | 75.36 | 122.65 | 124.05 | 125.00 | 100.27 | 113.49 | 117.25 | 127.41 | 104.23 | 119.41 | 143.02 | 125.49 |
Intensity Change Log 2 | — | 0.185352 | 0.565072 | 0.440509 | — | 0.045844 | 0.128324 | 0.219898 | — | 0.112417 | 0.347975 | 0.326721 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001066 | 0.004433 | 0.005696 | 0.012017 | 0.001950 | 0.002642 | 0.001163 | 0.000628 | 0.001818 | 0.003519 | 0.002944 | 0.010154 |
Bud Neck | 0.004436 | 0.001809 | 0.001365 | 0.004185 | 0.000473 | 0.000828 | 0.006911 | 0.003803 | 0.001064 | 0.001308 | 0.004732 | 0.004123 |
Bud Site | 0.002151 | 0.006791 | 0.011547 | 0.034384 | 0.001819 | 0.003799 | 0.004677 | 0.018494 | 0.001868 | 0.005263 | 0.007376 | 0.031785 |
Cell Periphery | 0.000197 | 0.000133 | 0.000040 | 0.000081 | 0.000039 | 0.000051 | 0.000136 | 0.000034 | 0.000062 | 0.000091 | 0.000099 | 0.000073 |
Cytoplasm | 0.009953 | 0.006954 | 0.008893 | 0.024681 | 0.004212 | 0.003310 | 0.027000 | 0.005742 | 0.005068 | 0.005093 | 0.019888 | 0.021583 |
Cytoplasmic Foci | 0.276624* | 0.288696* | 0.392245* | 0.301382* | 0.268617* | 0.269923* | 0.350500* | 0.292838* | 0.269810* | 0.279110* | 0.366897* | 0.299984* |
Eisosomes | 0.000179 | 0.000105 | 0.000029 | 0.000050 | 0.000067 | 0.000063 | 0.000341 | 0.000024 | 0.000084 | 0.000083 | 0.000218 | 0.000046 |
Endoplasmic Reticulum | 0.000099 | 0.000182 | 0.001263 | 0.000758 | 0.000369 | 0.000142 | 0.000359 | 0.000219 | 0.000329 | 0.000162 | 0.000714 | 0.000670 |
Endosome | 0.143332 | 0.201947* | 0.152240 | 0.188288 | 0.141035 | 0.223070* | 0.290889* | 0.313206* | 0.141378 | 0.212733* | 0.236428* | 0.208722* |
Golgi | 0.407892* | 0.383916* | 0.157990* | 0.052707 | 0.514540* | 0.392999* | 0.096386 | 0.041236 | 0.498643* | 0.388554* | 0.120584* | 0.050831 |
Lipid Particles | 0.016626 | 0.011198 | 0.010327 | 0.006632 | 0.010409 | 0.014266 | 0.045138 | 0.004426 | 0.011336 | 0.012765 | 0.031464 | 0.006271 |
Mitochondria | 0.025858 | 0.018913 | 0.001519 | 0.004299 | 0.017104 | 0.021128 | 0.005914 | 0.000592 | 0.018409 | 0.020044 | 0.004188 | 0.003692 |
Mitotic Spindle | 0.006482 | 0.009681 | 0.200775* | 0.146345* | 0.004699 | 0.009424 | 0.071026 | 0.147292* | 0.004964 | 0.009550 | 0.121991* | 0.146500* |
None | 0.000078 | 0.000062 | 0.000036 | 0.000065 | 0.000026 | 0.000045 | 0.004623 | 0.000026 | 0.000034 | 0.000054 | 0.002821 | 0.000059 |
Nuclear Periphery | 0.000037 | 0.000017 | 0.000332 | 0.000271 | 0.000027 | 0.000040 | 0.000272 | 0.000316 | 0.000029 | 0.000029 | 0.000296 | 0.000279 |
Nuclear Periphery Foci | 0.000240 | 0.000262 | 0.003428 | 0.002194 | 0.000156 | 0.000358 | 0.001613 | 0.002147 | 0.000168 | 0.000311 | 0.002326 | 0.002186 |
Nucleolus | 0.000081 | 0.000194 | 0.000175 | 0.000226 | 0.000038 | 0.000677 | 0.003203 | 0.000317 | 0.000045 | 0.000441 | 0.002014 | 0.000241 |
Nucleus | 0.000142 | 0.000203 | 0.000520 | 0.001433 | 0.000074 | 0.000742 | 0.012094 | 0.000254 | 0.000084 | 0.000478 | 0.007548 | 0.001240 |
Peroxisomes | 0.101904* | 0.058669 | 0.015414 | 0.140494* | 0.030318 | 0.047264 | 0.051095 | 0.110221* | 0.040989 | 0.052846 | 0.037079 | 0.135542* |
Vacuole | 0.001444 | 0.003578 | 0.032717 | 0.075090 | 0.002442 | 0.005112 | 0.025255 | 0.049380 | 0.002293 | 0.004361 | 0.028186 | 0.070885 |
Vacuole Periphery | 0.001178 | 0.002256 | 0.003447 | 0.004417 | 0.001587 | 0.004118 | 0.001405 | 0.008805 | 0.001526 | 0.003207 | 0.002207 | 0.005135 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.25 | -5.22 | -5.14 | -3.21 | -2.57 | -0.80 | 2.27 | 3.44 | 2.22 | 1.41 | -2.78 | -2.56 | -4.61 | -3.43 | -3.89 |
Bud Neck | 1.71 | 2.00 | -0.71 | -5.35 | -8.49 | -2.65 | -9.61 | -4.95 | -4.44 | 2.98 | -0.71 | -8.13 | -9.33 | -8.38 | -0.40 |
Bud Site | -3.55 | -8.87 | -8.76 | -7.25 | -5.99 | -2.00 | -4.21 | -3.15 | -2.81 | -2.65 | -4.14 | -9.20 | -9.37 | -8.20 | -7.55 |
Cell Periphery | 0.55 | 1.42 | 1.05 | 1.30 | -2.13 | -1.31 | -4.84 | 0.29 | 0.98 | 4.14 | -1.17 | -1.77 | -0.47 | 0.76 | 1.32 |
Cytoplasm | 0.93 | -0.15 | -5.20 | -6.79 | -6.01 | 0.96 | -9.93 | -1.95 | -2.40 | 6.25 | -0.01 | -9.69 | -7.36 | -7.42 | -2.83 |
Cytoplasmic Foci | -1.12 | -9.67 | 0.15 | 1.43 | 10.70 | -0.20 | -13.15 | -0.32 | -0.25 | 3.91 | -1.86 | -18.11 | -0.61 | 0.51 | 10.57 |
Eisosomes | 1.53 | 3.29 | 2.72 | 2.91 | -4.57 | 0.63 | -2.15 | 5.71 | 4.86 | 2.48 | 0.06 | -1.72 | 3.66 | 3.44 | 2.18 |
Endoplasmic Reticulum | -1.40 | -6.24 | -3.66 | -3.39 | 0.63 | 1.32 | -0.14 | 0.33 | -1.66 | 0.62 | 1.15 | -2.51 | -2.45 | -3.56 | -0.89 |
Endosome | -5.44 | -0.72 | -1.94 | 3.72 | -1.85 | -11.61 | -21.00 | -6.85 | -3.06 | -0.48 | -13.44 | -17.30 | -5.57 | 2.97 | 5.05 |
Golgi | 1.48 | 16.78 | 24.59 | 41.06* | 18.55* | 12.96 | 67.81* | 58.56* | 36.48* | 8.21 | 14.81 | 65.62* | 74.65* | 55.44* | 17.73 |
Lipid Particles | 1.37 | 1.65 | 2.58 | 1.99 | 2.22 | -1.60 | -10.83 | 3.90 | 4.08 | 13.00 | -0.84 | -9.18 | 3.32 | 3.74 | 12.17 |
Mitochondria | 1.45 | 5.71 | 4.84 | 5.49 | -1.14 | -1.52 | 6.73 | 10.68 | 9.55 | 8.16 | -0.77 | 9.41 | 7.40 | 8.00 | 0.72 |
Mitotic Spindle | -1.40 | -22.80* | -13.42 | -13.17 | 3.49 | -2.88 | -15.98 | -5.79 | -5.61 | -3.24 | -3.66 | -27.11 | -14.85 | -14.35 | -2.86 |
None | 0.44 | 1.13 | -0.96 | -1.18 | -1.44 | -1.84 | -4.20 | -0.18 | 1.28 | 4.19 | -1.77 | -4.18 | -1.47 | -1.22 | 3.99 |
Nuclear Periphery | 0.70 | -6.85 | -6.11 | -7.07 | -1.18 | -0.89 | -6.23 | -2.27 | -2.19 | -0.35 | -0.01 | -9.37 | -7.08 | -7.16 | -1.62 |
Nuclear Periphery Foci | -0.16 | -7.43 | -4.66 | -4.75 | 1.13 | -1.68 | -5.89 | -3.37 | -3.10 | -0.96 | -1.71 | -9.59 | -5.75 | -5.39 | -0.29 |
Nucleolus | -1.46 | -2.11 | -4.90 | -2.20 | -2.84 | -1.53 | -6.75 | -3.47 | 0.57 | 6.05 | -1.84 | -6.85 | -6.70 | 0.15 | 5.88 |
Nucleus | -0.82 | -3.25 | -3.49 | -3.42 | -2.85 | -1.23 | -8.74 | -3.09 | 0.62 | 8.49 | -1.41 | -8.93 | -3.60 | -2.96 | 4.04 |
Peroxisomes | 4.09 | 8.92 | -1.74 | -7.04 | -13.14 | -4.08 | -6.72 | -4.98 | -3.83 | -3.61 | -3.25 | 1.25 | -10.57 | -8.77 | -11.28 |
Vacuole | -3.62 | -13.35 | -14.24 | -13.98 | -9.41 | -3.63 | -13.53 | -4.77 | -4.57 | -2.75 | -4.03 | -18.69 | -14.84 | -14.56 | -10.49 |
Vacuole Periphery | -2.69 | -5.12 | -2.00 | -1.14 | -0.19 | -2.76 | 1.08 | -1.18 | -0.68 | -1.22 | -3.33 | -3.16 | -2.11 | -0.81 | -1.61 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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