Standard name | |
---|---|
Human Ortholog | |
Description | Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 4 | 6 | 2 | 3 | 7 | 7 | 3 | 1 | 4 | 2 | 3 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 2 | 2 | 6 | 14 |
Bud | 4 | 9 | 10 | 4 | 9 | 26 | 20 | 32 | 25 | 31 | 14 | 27 | 0 | 2 | 1 | 1 | 1 | 8 | 8 | 3 | 4 | 5 | 16 | 19 |
Bud Neck | 3 | 4 | 8 | 9 | 9 | 24 | 17 | 23 | 12 | 9 | 6 | 6 | 2 | 0 | 0 | 0 | 1 | 3 | 1 | 1 | 0 | 3 | 8 | 19 |
Bud Site | 0 | 4 | 1 | 4 | 2 | 15 | 7 | 14 | 17 | 16 | 10 | 13 | 0 | 1 | 0 | 3 | 0 | 2 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 32 | 61 | 57 | 38 | 30 | 32 | 61 | 80 | 47 | 37 | 35 | 27 | 107 | 192 | 232 | 150 | 157 | 158 | 22 | 42 | 28 | 56 | 83 | 111 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 1 | 1 | 23 | 22 | 22 | 2 | 1 | 1 | 0 | 1 | 4 |
Endosome | 228 | 180 | 54 | 60 | 24 | 8 | 54 | 34 | 30 | 29 | 29 | 58 | 224 | 295 | 304 | 138 | 111 | 90 | 39 | 82 | 28 | 39 | 57 | 78 |
Golgi | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 2 | 4 |
Mitochondria | 14 | 23 | 0 | 4 | 3 | 49 | 19 | 42 | 52 | 57 | 56 | 56 | 2 | 1 | 1 | 23 | 14 | 31 | 4 | 14 | 8 | 7 | 11 | 12 |
Nucleus | 1 | 5 | 0 | 2 | 5 | 7 | 7 | 8 | 9 | 8 | 8 | 5 | 1 | 1 | 2 | 14 | 17 | 13 | 2 | 3 | 1 | 1 | 3 | 5 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 |
Nucleolus | 38 | 34 | 5 | 6 | 6 | 27 | 29 | 24 | 22 | 22 | 16 | 19 | 11 | 12 | 12 | 29 | 28 | 35 | 3 | 5 | 5 | 1 | 2 | 4 |
Peroxisomes | 13 | 2 | 2 | 0 | 0 | 0 | 5 | 4 | 4 | 8 | 3 | 8 | 3 | 5 | 12 | 3 | 4 | 4 | 0 | 1 | 1 | 1 | 1 | 1 |
SpindlePole | 168 | 198 | 240 | 126 | 150 | 134 | 234 | 232 | 158 | 141 | 85 | 153 | 149 | 148 | 165 | 168 | 108 | 116 | 288 | 344 | 301 | 199 | 300 | 244 |
Vac/Vac Membrane | 36 | 29 | 12 | 4 | 8 | 2 | 9 | 5 | 5 | 8 | 7 | 9 | 39 | 43 | 34 | 27 | 36 | 37 | 3 | 9 | 4 | 6 | 6 | 10 |
Unique Cell Count | 351 | 417 | 334 | 206 | 207 | 228 | 346 | 387 | 275 | 242 | 196 | 264 | 403 | 546 | 586 | 447 | 374 | 376 | 386 | 526 | 399 | 338 | 518 | 546 |
Labelled Cell Count | 540 | 557 | 395 | 259 | 249 | 331 | 469 | 502 | 382 | 370 | 272 | 384 | 545 | 701 | 765 | 582 | 500 | 523 | 386 | 526 | 399 | 338 | 518 | 546 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.3 | 5.8 | 5.8 | 6.4 | 4.1 | 5.3 | 5.2 | 4.8 | 5.0 | 4.9 | 5.0 | 5.8 | 5.9 | 6.1 | 7.5 | 7.4 | 6.3 | 5.2 | 5.2 | 5.4 |
Std Deviation (1e-4) | 0.7 | 0.8 | 0.8 | 1.5 | 2.2 | 1.3 | 1.6 | 1.5 | 2.0 | 1.5 | 1.8 | 1.1 | 1.0 | 1.4 | 1.5 | 1.7 | 1.8 | 1.4 | 1.2 | 1.2 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.14 | -0.5 | -0.15 | -0.16 | -0.28 | -0.23 | -0.26 | -0.22 | -0.01 | 0.02 | 0.07 | 0.36 | 0.33 | 0.12 | -0.17 | -0.15 | -0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -0.7 | 0.8 | 0 | 1.8 | 3.0 | 0 | 4.5 | 0 | 3.6 | 0 | 0 | 0 | 0 | -2.9 | -0.7 |
Bud Neck | 1.3 | 1.3 | 0 | 1.7 | 2.3 | 0 | 0.9 | 0 | -0.1 | 0 | 0 | 0 | 0 | 0 | -1.7 |
Bud Site | 0 | 0 | 0 | 0 | 3.1 | 0 | 4.4 | 0 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.4 | -0.8 | -1.0 | 0.2 | 1.2 | 0 | -0.6 | 0.2 | -2.4 | 3.1 | 5.8 | 7.1 | 5.2 | 7.2 | 7.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.2 | 4.5 | 4.5 |
Endosome | 3.6 | -1.5 | 0 | -0.2 | -3.0 | 0 | -1.4 | 0 | 1.8 | 11.0 | 11.1 | 10.7 | 4.7 | 4.2 | 2.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 4.3 | 6.2 | 0 | 9.3 | 0 | 8.8 | 0 | 0 | 0 | 4.2 | 3.6 | 5.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 3.9 | 3.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 4.1 | 3.2 | 0 | 4.3 | 0 | 3.5 | 1.1 | 0.7 | 0.6 | 3.4 | 3.8 | 4.5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 0 | 0 |
SpindlePole | -2.6 | 0.2 | 0 | -1.2 | -3.4 | 0 | -3.4 | 0 | -3.6 | -9.4 | -13.0 | -12.8 | -9.5 | -11.4 | -10.9 |
Vacuole | -1.1 | 0.2 | -2.0 | -0.7 | -2.0 | -1.3 | -0.2 | 0 | -0.1 | 3.2 | 2.5 | 1.5 | 1.6 | 3.2 | 3.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.1444 | 0.4913 | 0.1894 | -0.1713 | -0.126 | 0.0379 | 2.2964 | 1.8828 | 1.9128 | 1.7897 | 1.6187 | 1.7021 | 3.3785 | 4.6843 | 4.0348 | 4.202 | 3.5801 | 4.0409 |
Actin | 0.0278 | 0.001 | 0.0163 | 0.0016 | 0.0091 | 0.0028 | 0.0112 | 0.0012 | 0.0016 | 0.0059 | 0.0013 | 0.0009 | 0.0011 | 0.0007 | 0.0005 | 0.0014 | 0.0001 | 0.001 |
Bud | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.001 | 0.0006 | 0.0021 | 0.0017 | 0.0014 | 0.0003 | 0.0005 | 0.0004 | 0.0016 | 0 | 0 | 0 | 0.0001 |
Bud Neck | 0.0027 | 0.0016 | 0.0014 | 0.0068 | 0.0026 | 0.0028 | 0.0065 | 0.0025 | 0.0036 | 0.0575 | 0.0026 | 0.0023 | 0.0008 | 0.0008 | 0.001 | 0.0022 | 0.0301 | 0.0016 |
Bud Periphery | 0.0005 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0004 | 0.0014 | 0.0007 | 0.0009 | 0.0017 | 0.0007 | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0 |
Bud Site | 0.0039 | 0.0069 | 0.0037 | 0.0003 | 0.0005 | 0.0004 | 0.0094 | 0.0156 | 0.0035 | 0.0037 | 0.003 | 0.0005 | 0.0035 | 0.0052 | 0.0006 | 0.0002 | 0.0004 | 0.0002 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0482 | 0.1771 | 0.0291 | 0.0571 | 0.0388 | 0.0376 | 0.0685 | 0.2633 | 0.085 | 0.0701 | 0.0273 | 0.0514 | 0.0257 | 0.1414 | 0.0403 | 0.0242 | 0.0149 | 0.0337 |
Cytoplasmic Foci | 0.1556 | 0.1338 | 0.0905 | 0.2175 | 0.2941 | 0.1131 | 0.0904 | 0.0852 | 0.09 | 0.1625 | 0.2053 | 0.0806 | 0.2583 | 0.1712 | 0.1604 | 0.2142 | 0.3516 | 0.1678 |
Eisosomes | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0006 | 0 | 0.0007 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0036 | 0.0045 | 0.0014 | 0.0007 | 0.0008 | 0.0013 | 0.0048 | 0.0034 | 0.0015 | 0.0039 | 0.0007 | 0.0009 | 0.0008 | 0.0032 | 0.0014 | 0.0006 | 0.0002 | 0.0008 |
Endosome | 0.0536 | 0.0202 | 0.0172 | 0.018 | 0.0374 | 0.0166 | 0.047 | 0.0248 | 0.0247 | 0.0363 | 0.0495 | 0.0196 | 0.0168 | 0.0231 | 0.0141 | 0.0049 | 0.0081 | 0.0128 |
Golgi | 0.0124 | 0.0015 | 0.0035 | 0.0057 | 0.0042 | 0.0019 | 0.0079 | 0.0021 | 0.0055 | 0.0065 | 0.0061 | 0.0021 | 0.0021 | 0.0017 | 0.0015 | 0.0019 | 0.002 | 0.0014 |
Lipid Particles | 0.0514 | 0.0056 | 0.0118 | 0.032 | 0.0638 | 0.0207 | 0.0394 | 0.0032 | 0.0069 | 0.0149 | 0.0303 | 0.0085 | 0.0098 | 0.0048 | 0.0136 | 0.007 | 0.0073 | 0.01 |
Mitochondria | 0.012 | 0.0006 | 0.0159 | 0.0022 | 0.0022 | 0.0009 | 0.0241 | 0.0013 | 0.0033 | 0.0305 | 0.0201 | 0.0022 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0002 |
None | 0.0647 | 0.2088 | 0.0394 | 0.1101 | 0.0569 | 0.0599 | 0.0317 | 0.1171 | 0.0525 | 0.0532 | 0.0655 | 0.0475 | 0.0235 | 0.1224 | 0.0529 | 0.0882 | 0.0322 | 0.0482 |
Nuclear Periphery | 0.0198 | 0.0116 | 0.0094 | 0.004 | 0.0028 | 0.009 | 0.0427 | 0.0085 | 0.0078 | 0.0106 | 0.0027 | 0.0052 | 0.0023 | 0.0064 | 0.0031 | 0.0016 | 0.0007 | 0.0035 |
Nucleolus | 0.006 | 0.0046 | 0.0074 | 0.0039 | 0.0294 | 0.0066 | 0.0095 | 0.0066 | 0.006 | 0.0041 | 0.0232 | 0.0054 | 0.0019 | 0.0025 | 0.0023 | 0.0013 | 0.0003 | 0.0016 |
Nucleus | 0.033 | 0.0599 | 0.0449 | 0.02 | 0.0089 | 0.0348 | 0.0672 | 0.1246 | 0.1008 | 0.0689 | 0.0079 | 0.055 | 0.0085 | 0.053 | 0.0262 | 0.0042 | 0.0011 | 0.0299 |
Peroxisomes | 0.0261 | 0.0052 | 0.0128 | 0.0373 | 0.0383 | 0.0113 | 0.0408 | 0.0076 | 0.021 | 0.0511 | 0.0172 | 0.0097 | 0.0073 | 0.004 | 0.0049 | 0.0137 | 0.0141 | 0.0084 |
Punctate Nuclear | 0.4747 | 0.3542 | 0.6895 | 0.4821 | 0.407 | 0.677 | 0.4859 | 0.3247 | 0.5804 | 0.412 | 0.5172 | 0.7059 | 0.6362 | 0.4545 | 0.6763 | 0.6338 | 0.5365 | 0.6785 |
Vacuole | 0.0019 | 0.002 | 0.0042 | 0.0004 | 0.0013 | 0.0012 | 0.0059 | 0.0047 | 0.0023 | 0.0036 | 0.0153 | 0.0013 | 0.0005 | 0.0021 | 0.0006 | 0.0002 | 0.0001 | 0.0003 |
Vacuole Periphery | 0.0013 | 0.0004 | 0.0004 | 0.0002 | 0.0007 | 0.0005 | 0.0041 | 0.0006 | 0.0008 | 0.0013 | 0.0033 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.195 | 4.1099 | 9.1492 | 6.9529 | 3.9739 | 9.1899 | 7.2722 | 8.4141 | 10.0042 | 9.2882 |
Translational Efficiency | 0.3895 | 1.2454 | 0.5263 | 0.496 | 0.9156 | 0.5785 | 0.6888 | 0.8242 | 0.5408 | 0.5565 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2595 | 1061 | 1593 | 1905 | 1669 | 2204 | 2345 | 1704 | 4264 | 3265 | 3938 | 3609 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 736.44 | 749.26 | 889.89 | 839.00 | 675.40 | 800.82 | 852.91 | 834.19 | 712.55 | 784.06 | 867.87 | 836.73 |
Standard Deviation | 85.81 | 129.99 | 106.00 | 120.43 | 78.15 | 98.88 | 106.69 | 128.17 | 88.09 | 112.58 | 107.94 | 124.17 |
Intensity Change Log 2 | — | 0.024898 | 0.273059 | 0.188103 | — | 0.245736 | 0.336651 | 0.304634 | — | 0.134766 | 0.303830 | 0.245025 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000149 | 0.000466 | 0.000665 | 0.002196 | 0.000222 | 0.000384 | 0.000258 | 0.000498 | 0.000178 | 0.000411 | 0.000423 | 0.001394 |
Bud Neck | 0.014429 | 0.008840 | 0.007868 | 0.011403 | 0.010142 | 0.016017 | 0.004570 | 0.009065 | 0.012751 | 0.013685 | 0.005904 | 0.010299 |
Bud Site | 0.005715 | 0.009309 | 0.025898 | 0.053804 | 0.004707 | 0.018678 | 0.006747 | 0.049354 | 0.005321 | 0.015633 | 0.014494 | 0.051703 |
Cell Periphery | 0.000129 | 0.000142 | 0.000137 | 0.000379 | 0.000137 | 0.000111 | 0.000140 | 0.000203 | 0.000132 | 0.000121 | 0.000139 | 0.000296 |
Cytoplasm | 0.191785* | 0.151961* | 0.210038* | 0.265554* | 0.220238* | 0.227274* | 0.262831* | 0.485370* | 0.202922* | 0.202800* | 0.241475* | 0.369341* |
Cytoplasmic Foci | 0.365503* | 0.267218* | 0.016259 | 0.032261 | 0.346342* | 0.256359* | 0.019634 | 0.041883 | 0.358004* | 0.259888* | 0.018269 | 0.036804 |
Eisosomes | 0.000088 | 0.000093 | 0.000051 | 0.000028 | 0.000099 | 0.000057 | 0.000042 | 0.000051 | 0.000092 | 0.000068 | 0.000045 | 0.000039 |
Endoplasmic Reticulum | 0.000942 | 0.001731 | 0.002917 | 0.000891 | 0.001018 | 0.001031 | 0.001152 | 0.001314 | 0.000972 | 0.001258 | 0.001866 | 0.001091 |
Endosome | 0.023920 | 0.030915 | 0.005863 | 0.017193 | 0.018882 | 0.035282 | 0.004494 | 0.011752 | 0.021948 | 0.033863 | 0.005048 | 0.014624 |
Golgi | 0.007986 | 0.013146 | 0.000576 | 0.009860 | 0.007489 | 0.009114 | 0.002640 | 0.003959 | 0.007791 | 0.010424 | 0.001805 | 0.007074 |
Lipid Particles | 0.068653 | 0.026120 | 0.002043 | 0.001579 | 0.028640 | 0.018582 | 0.001695 | 0.001512 | 0.052991 | 0.021032 | 0.001836 | 0.001547 |
Mitochondria | 0.007715 | 0.007696 | 0.001393 | 0.008637 | 0.004783 | 0.011899 | 0.001232 | 0.002957 | 0.006567 | 0.010533 | 0.001297 | 0.005955 |
Mitotic Spindle | 0.149233* | 0.306176* | 0.417745* | 0.443450* | 0.131029* | 0.262627* | 0.385500* | 0.157836* | 0.142107* | 0.276779* | 0.398544* | 0.308596* |
None | 0.013675 | 0.030359 | 0.014023 | 0.009361 | 0.020056 | 0.010159 | 0.014603 | 0.010776 | 0.016172 | 0.016723 | 0.014368 | 0.010029 |
Nuclear Periphery | 0.001369 | 0.000721 | 0.001786 | 0.001310 | 0.001044 | 0.000921 | 0.001267 | 0.001908 | 0.001242 | 0.000856 | 0.001477 | 0.001592 |
Nuclear Periphery Foci | 0.014921 | 0.017598 | 0.012240 | 0.003463 | 0.020536 | 0.007905 | 0.005990 | 0.001781 | 0.017119 | 0.011055 | 0.008518 | 0.002668 |
Nucleolus | 0.042414 | 0.023153 | 0.015765 | 0.002435 | 0.043205 | 0.021499 | 0.015105 | 0.004171 | 0.042724 | 0.022036 | 0.015372 | 0.003255 |
Nucleus | 0.061184 | 0.058189 | 0.229946* | 0.075384 | 0.103394 | 0.059766 | 0.250129* | 0.135787* | 0.077706 | 0.059254 | 0.241964* | 0.103903 |
Peroxisomes | 0.023674 | 0.029698 | 0.003393 | 0.005399 | 0.031181 | 0.029223 | 0.001638 | 0.005286 | 0.026612 | 0.029377 | 0.002348 | 0.005346 |
Vacuole | 0.005942 | 0.014848 | 0.031038 | 0.048486 | 0.006430 | 0.012000 | 0.019283 | 0.071025 | 0.006133 | 0.012926 | 0.024038 | 0.059128 |
Vacuole Periphery | 0.000574 | 0.001619 | 0.000356 | 0.006927 | 0.000426 | 0.001112 | 0.001049 | 0.003513 | 0.000516 | 0.001277 | 0.000769 | 0.005315 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -8.44 | -16.12 | -4.10 | -2.49 | 0.28 | -2.05 | -11.29 | -9.85 | -8.32 | -0.54 | -8.99 | -20.31 | -5.66 | -4.17 | -0.33 |
Bud Neck | 0.87 | -8.54 | -7.66 | -7.42 | 1.02 | -6.14 | -8.19 | -8.81 | -2.61 | -2.64 | -4.67 | -11.25 | -11.36 | -5.63 | -1.47 |
Bud Site | -5.44 | -10.35 | -13.01 | -9.81 | -5.31 | -5.42 | -11.86 | -11.71 | -8.56 | -7.65 | -7.21 | -15.67 | -17.39 | -12.33 | -9.57 |
Cell Periphery | -4.53 | -6.43 | -3.87 | -2.07 | -1.75 | 3.36 | -1.44 | -4.86 | -7.21 | -4.03 | -0.51 | -5.94 | -5.47 | -5.19 | -3.27 |
Cytoplasm | -1.81 | 0.27 | -14.35 | -10.33 | -14.07 | -5.14 | -1.23 | -24.38 | -20.10 | -24.23 | -6.35 | -1.48 | -27.62 | -20.19 | -26.35 |
Cytoplasmic Foci | 12.85 | 63.04 | 58.22 | 28.56 | -7.82 | 4.50 | 44.04 | 37.30 | 35.97 | -8.29 | 12.45 | 75.29 | 67.51 | 47.89 | -10.05 |
Eisosomes | -2.37 | -3.69 | 3.81 | 4.58 | 5.87 | 12.10 | 6.11 | 1.67 | -8.29 | -3.68 | 6.85 | 0.93 | 3.14 | -2.95 | 1.79 |
Endoplasmic Reticulum | -3.28 | -17.10 | -9.72 | -2.89 | 11.41 | 0.14 | -11.97 | -6.59 | -5.36 | 3.85 | -2.10 | -20.33 | -11.50 | -6.70 | 10.80 |
Endosome | -4.71 | 12.22 | 9.99 | 11.50 | -0.89 | -5.53 | 12.02 | 7.31 | 11.50 | -4.06 | -6.96 | 17.61 | 12.37 | 15.96 | -3.48 |
Golgi | -4.30 | 13.70 | 5.45 | 7.65 | -2.09 | 1.94 | 11.51 | 9.62 | 6.67 | -0.74 | -1.29 | 16.23 | 9.67 | 9.22 | -1.96 |
Lipid Particles | 11.04 | 22.22 | 22.30 | 12.62 | 0.60 | 5.64 | 17.23 | 16.71 | 12.01 | -2.03 | 13.85 | 26.80 | 26.63 | 17.19 | -1.20 |
Mitochondria | -0.72 | 9.04 | 1.90 | 1.96 | -2.14 | -3.27 | 9.04 | 4.23 | 5.15 | -1.64 | -2.93 | 12.65 | 3.76 | 4.88 | -2.75 |
Mitotic Spindle | -13.43* | -26.28* | -26.92* | -8.27 | 0.84 | -8.75 | -28.15* | -5.18 | 2.79 | 21.11* | -16.23 | -40.12 | -23.04 | -6.65 | 14.15 |
None | -2.96 | 0.81 | 5.04 | 4.64 | 3.86 | 6.26 | 5.70 | 6.47 | 0.30 | 1.78 | 1.16 | 4.62 | 7.95 | 4.24 | 4.14 |
Nuclear Periphery | 6.52 | -16.29 | -12.83 | -15.34 | 0.56 | -0.79 | -14.35 | -5.94 | -5.72 | 0.38 | 4.26 | -20.81 | -12.30 | -13.69 | -0.10 |
Nuclear Periphery Foci | 0.64 | 2.31 | 11.56 | 7.82 | 8.04 | 9.05 | 4.03 | 12.49 | 5.24 | 10.22 | 8.36 | 4.36 | 17.10 | 8.67 | 12.78 |
Nucleolus | 13.45* | 9.41 | 24.90* | 7.73 | 14.08 | 8.18 | 7.26 | 17.56 | 10.65 | 12.09 | 14.11* | 11.68 | 29.87* | 14.35 | 18.80 |
Nucleus | -2.78 | -23.42 | -17.67 | -12.44 | 10.99 | 2.66 | -15.97 | -16.32 | -19.27 | 0.17 | -1.06 | -28.90 | -24.64 | -22.79 | 7.86 |
Peroxisomes | -2.84 | 24.55 | 19.34 | 12.65 | -3.71 | 2.08 | 20.81 | 13.86 | 12.40 | -5.41 | -1.22 | 31.77 | 22.50 | 19.01 | -6.28 |
Vacuole | -5.93 | -14.17 | -21.66 | -18.97 | -11.12 | -5.21 | -12.38 | -21.55 | -20.22 | -15.49 | -8.30 | -18.94 | -30.58 | -27.96 | -19.30 |
Vacuole Periphery | -4.83 | -2.14 | -2.86 | -1.32 | -2.73 | -2.63 | -1.20 | -4.11 | -2.60 | -3.64 | -4.66 | -1.62 | -4.31 | -2.62 | -4.02 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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