Standard name
Human Ortholog
Description Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.11 0.06 0.08 0.09 0.13 0.07 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0.11 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.07 0 0 0.06 0.07 0.05 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.15 0.17 0.18 0.14 0.14 0.18 0.21 0.17 0.15 0.18 0.1 0.27 0.35 0.4 0.34 0.42 0.42 0.06 0.08 0.07 0.17 0.16 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0 0 0 0 0
Endosome 0.65 0.43 0.16 0.29 0.12 0 0.16 0.09 0.11 0.12 0.15 0.22 0.56 0.54 0.52 0.31 0.3 0.24 0.1 0.16 0.07 0.12 0.11 0.14
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0 0 0.21 0.05 0.11 0.19 0.24 0.29 0.21 0 0 0 0.05 0 0.08 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.08 0 0 0 0.12 0.08 0.06 0.08 0.09 0.08 0.07 0 0 0 0.06 0.07 0.09 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.48 0.47 0.72 0.61 0.72 0.59 0.68 0.6 0.57 0.58 0.43 0.58 0.37 0.27 0.28 0.38 0.29 0.31 0.75 0.65 0.76 0.59 0.58 0.45
Vac/Vac Membrane 0.1 0.07 0 0 0 0 0 0 0 0 0 0 0.1 0.08 0.06 0.06 0.1 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 6 2 3 7 7 3 1 4 2 3 1 0 0 1 0 1 1 2 2 2 6 14
Bud 4 9 10 4 9 26 20 32 25 31 14 27 0 2 1 1 1 8 8 3 4 5 16 19
Bud Neck 3 4 8 9 9 24 17 23 12 9 6 6 2 0 0 0 1 3 1 1 0 3 8 19
Bud Site 0 4 1 4 2 15 7 14 17 16 10 13 0 1 0 3 0 2 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 2 1 2 0 1 0 0 0 0
Cytoplasm 32 61 57 38 30 32 61 80 47 37 35 27 107 192 232 150 157 158 22 42 28 56 83 111
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 1 0 4 1 1 23 22 22 2 1 1 0 1 4
Endosome 228 180 54 60 24 8 54 34 30 29 29 58 224 295 304 138 111 90 39 82 28 39 57 78
Golgi 2 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 1 2 1 2 4
Mitochondria 14 23 0 4 3 49 19 42 52 57 56 56 2 1 1 23 14 31 4 14 8 7 11 12
Nucleus 1 5 0 2 5 7 7 8 9 8 8 5 1 1 2 14 17 13 2 3 1 1 3 5
Nuclear Periphery 0 2 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 1 1 1
Nucleolus 38 34 5 6 6 27 29 24 22 22 16 19 11 12 12 29 28 35 3 5 5 1 2 4
Peroxisomes 13 2 2 0 0 0 5 4 4 8 3 8 3 5 12 3 4 4 0 1 1 1 1 1
SpindlePole 168 198 240 126 150 134 234 232 158 141 85 153 149 148 165 168 108 116 288 344 301 199 300 244
Vac/Vac Membrane 36 29 12 4 8 2 9 5 5 8 7 9 39 43 34 27 36 37 3 9 4 6 6 10
Unique Cell Count 351 417 334 206 207 228 346 387 275 242 196 264 403 546 586 447 374 376 386 526 399 338 518 546
Labelled Cell Count 540 557 395 259 249 331 469 502 382 370 272 384 545 701 765 582 500 523 386 526 399 338 518 546


Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.3 5.8 5.8 6.4 4.1 5.3 5.2 4.8 5.0 4.9 5.0 5.8 5.9 6.1 7.5 7.4 6.3 5.2 5.2 5.4
Std Deviation (1e-4) 0.7 0.8 0.8 1.5 2.2 1.3 1.6 1.5 2.0 1.5 1.8 1.1 1.0 1.4 1.5 1.7 1.8 1.4 1.2 1.2 1.6
Intensity Change (Log2) – – – -0.01 0.14 -0.5 -0.15 -0.16 -0.28 -0.23 -0.26 -0.22 -0.01 0.02 0.07 0.36 0.33 0.12 -0.17 -0.15 -0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0.2 0 0 0 0 0 0 0 0 0 0 0
Bud -0.7 0.8 0 1.8 3.0 0 4.5 0 3.6 0 0 0 0 -2.9 -0.7
Bud Neck 1.3 1.3 0 1.7 2.3 0 0.9 0 -0.1 0 0 0 0 0 -1.7
Bud Site 0 0 0 0 3.1 0 4.4 0 3.7 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 -0.8 -1.0 0.2 1.2 0 -0.6 0.2 -2.4 3.1 5.8 7.1 5.2 7.2 7.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 4.2 4.5 4.5
Endosome 3.6 -1.5 0 -0.2 -3.0 0 -1.4 0 1.8 11.0 11.1 10.7 4.7 4.2 2.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.3 6.2 0 9.3 0 8.8 0 0 0 4.2 3.6 5.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 3.3 3.9 3.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 4.1 3.2 0 4.3 0 3.5 1.1 0.7 0.6 3.4 3.8 4.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1.7 0 0 0
SpindlePole -2.6 0.2 0 -1.2 -3.4 0 -3.4 0 -3.6 -9.4 -13.0 -12.8 -9.5 -11.4 -10.9
Vacuole -1.1 0.2 -2.0 -0.7 -2.0 -1.3 -0.2 0 -0.1 3.2 2.5 1.5 1.6 3.2 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1444 0.4913 0.1894 -0.1713 -0.126 0.0379 2.2964 1.8828 1.9128 1.7897 1.6187 1.7021 3.3785 4.6843 4.0348 4.202 3.5801 4.0409
Actin 0.0278 0.001 0.0163 0.0016 0.0091 0.0028 0.0112 0.0012 0.0016 0.0059 0.0013 0.0009 0.0011 0.0007 0.0005 0.0014 0.0001 0.001
Bud 0.0003 0.0002 0.0004 0.0001 0.0002 0.001 0.0006 0.0021 0.0017 0.0014 0.0003 0.0005 0.0004 0.0016 0 0 0 0.0001
Bud Neck 0.0027 0.0016 0.0014 0.0068 0.0026 0.0028 0.0065 0.0025 0.0036 0.0575 0.0026 0.0023 0.0008 0.0008 0.001 0.0022 0.0301 0.0016
Bud Periphery 0.0005 0.0001 0.0003 0 0.0002 0.0004 0.0014 0.0007 0.0009 0.0017 0.0007 0.0003 0.0001 0.0004 0 0.0001 0 0
Bud Site 0.0039 0.0069 0.0037 0.0003 0.0005 0.0004 0.0094 0.0156 0.0035 0.0037 0.003 0.0005 0.0035 0.0052 0.0006 0.0002 0.0004 0.0002
Cell Periphery 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0004 0.0001 0.0001 0.0002 0.0002 0 0.0001 0.0001 0 0 0 0
Cytoplasm 0.0482 0.1771 0.0291 0.0571 0.0388 0.0376 0.0685 0.2633 0.085 0.0701 0.0273 0.0514 0.0257 0.1414 0.0403 0.0242 0.0149 0.0337
Cytoplasmic Foci 0.1556 0.1338 0.0905 0.2175 0.2941 0.1131 0.0904 0.0852 0.09 0.1625 0.2053 0.0806 0.2583 0.1712 0.1604 0.2142 0.3516 0.1678
Eisosomes 0.0005 0.0001 0.0002 0 0.0006 0 0.0007 0 0 0.0001 0.0001 0 0 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0036 0.0045 0.0014 0.0007 0.0008 0.0013 0.0048 0.0034 0.0015 0.0039 0.0007 0.0009 0.0008 0.0032 0.0014 0.0006 0.0002 0.0008
Endosome 0.0536 0.0202 0.0172 0.018 0.0374 0.0166 0.047 0.0248 0.0247 0.0363 0.0495 0.0196 0.0168 0.0231 0.0141 0.0049 0.0081 0.0128
Golgi 0.0124 0.0015 0.0035 0.0057 0.0042 0.0019 0.0079 0.0021 0.0055 0.0065 0.0061 0.0021 0.0021 0.0017 0.0015 0.0019 0.002 0.0014
Lipid Particles 0.0514 0.0056 0.0118 0.032 0.0638 0.0207 0.0394 0.0032 0.0069 0.0149 0.0303 0.0085 0.0098 0.0048 0.0136 0.007 0.0073 0.01
Mitochondria 0.012 0.0006 0.0159 0.0022 0.0022 0.0009 0.0241 0.0013 0.0033 0.0305 0.0201 0.0022 0.0002 0.0006 0.0001 0.0002 0 0.0002
None 0.0647 0.2088 0.0394 0.1101 0.0569 0.0599 0.0317 0.1171 0.0525 0.0532 0.0655 0.0475 0.0235 0.1224 0.0529 0.0882 0.0322 0.0482
Nuclear Periphery 0.0198 0.0116 0.0094 0.004 0.0028 0.009 0.0427 0.0085 0.0078 0.0106 0.0027 0.0052 0.0023 0.0064 0.0031 0.0016 0.0007 0.0035
Nucleolus 0.006 0.0046 0.0074 0.0039 0.0294 0.0066 0.0095 0.0066 0.006 0.0041 0.0232 0.0054 0.0019 0.0025 0.0023 0.0013 0.0003 0.0016
Nucleus 0.033 0.0599 0.0449 0.02 0.0089 0.0348 0.0672 0.1246 0.1008 0.0689 0.0079 0.055 0.0085 0.053 0.0262 0.0042 0.0011 0.0299
Peroxisomes 0.0261 0.0052 0.0128 0.0373 0.0383 0.0113 0.0408 0.0076 0.021 0.0511 0.0172 0.0097 0.0073 0.004 0.0049 0.0137 0.0141 0.0084
Punctate Nuclear 0.4747 0.3542 0.6895 0.4821 0.407 0.677 0.4859 0.3247 0.5804 0.412 0.5172 0.7059 0.6362 0.4545 0.6763 0.6338 0.5365 0.6785
Vacuole 0.0019 0.002 0.0042 0.0004 0.0013 0.0012 0.0059 0.0047 0.0023 0.0036 0.0153 0.0013 0.0005 0.0021 0.0006 0.0002 0.0001 0.0003
Vacuole Periphery 0.0013 0.0004 0.0004 0.0002 0.0007 0.0005 0.0041 0.0006 0.0008 0.0013 0.0033 0.0003 0.0001 0.0003 0.0001 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.195 4.1099 9.1492 6.9529 3.9739 9.1899 7.2722 8.4141 10.0042 9.2882
Translational Efficiency 0.3895 1.2454 0.5263 0.496 0.9156 0.5785 0.6888 0.8242 0.5408 0.5565

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4
Localization
Cell Percentages spindle pole (38%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mcm22

Mcm22


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mcm22-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available