Standard name | |
---|---|
Human Ortholog | |
Description | Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 9 | 4 | 3 | 5 | 8 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 17 | 16 | 5 | 12 | 12 | 23 |
Bud Neck | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 3 | 5 |
Bud Site | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 1 | 3 | 5 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 20 | 18 | 19 | 19 | 54 | 50 | 36 | 53 | 34 | 43 | 21 | 26 | 39 | 46 | 60 | 63 | 34 | 64 | 13 | 13 | 13 | 15 | 13 | 22 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 24 | 12 | 21 | 0 | 0 | 1 | 1 | 1 | 4 |
Endosome | 4 | 4 | 2 | 5 | 6 | 3 | 0 | 1 | 0 | 2 | 0 | 0 | 2 | 6 | 11 | 54 | 25 | 40 | 10 | 6 | 3 | 15 | 30 | 43 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 5 | 2 | 1 | 1 | 8 | 9 |
Mitochondria | 31 | 2 | 0 | 2 | 4 | 53 | 103 | 70 | 124 | 130 | 141 | 89 | 2 | 0 | 0 | 20 | 6 | 5 | 3 | 2 | 6 | 4 | 21 | 21 |
Nucleus | 226 | 150 | 142 | 119 | 87 | 66 | 88 | 74 | 51 | 59 | 50 | 64 | 144 | 141 | 178 | 162 | 58 | 109 | 249 | 213 | 139 | 120 | 138 | 125 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 |
Nucleolus | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 1 | 33 | 12 | 19 | 10 | 9 | 2 | 16 | 14 | 12 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 3 | 7 |
SpindlePole | 2 | 1 | 1 | 1 | 4 | 0 | 0 | 1 | 1 | 4 | 0 | 4 | 2 | 0 | 2 | 21 | 4 | 7 | 15 | 9 | 6 | 13 | 29 | 38 |
Vac/Vac Membrane | 89 | 112 | 22 | 32 | 34 | 32 | 39 | 24 | 23 | 25 | 15 | 29 | 31 | 44 | 41 | 191 | 90 | 137 | 40 | 34 | 33 | 36 | 63 | 71 |
Unique Cell Count | 331 | 263 | 180 | 168 | 181 | 172 | 216 | 191 | 185 | 215 | 183 | 172 | 210 | 221 | 277 | 449 | 199 | 345 | 375 | 313 | 218 | 249 | 356 | 405 |
Labelled Cell Count | 375 | 291 | 187 | 180 | 192 | 207 | 281 | 229 | 242 | 276 | 241 | 221 | 221 | 237 | 294 | 573 | 243 | 407 | 375 | 313 | 218 | 249 | 356 | 405 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.9 | 7.7 | 7.0 | 6.4 | 5.5 | 4.5 | 4.4 | 4.9 | 4.0 | 4.3 | 3.9 | 4.2 | 7.4 | 7.2 | 7.2 | 9.0 | 9.6 | 8.9 | 7.2 | 6.7 | 6.9 |
Std Deviation (1e-4) | 1.5 | 2.0 | 1.5 | 1.2 | 1.5 | 1.2 | 1.1 | 1.4 | 0.9 | 1.1 | 1.2 | 1.0 | 1.6 | 1.4 | 1.7 | 2.6 | 2.8 | 2.5 | 2.2 | 1.8 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.35 | -0.64 | -0.68 | -0.53 | -0.83 | -0.7 | -0.85 | -0.76 | 0.08 | 0.04 | 0.02 | 0.35 | 0.44 | 0.33 | 0.03 | -0.07 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | 4.6 | 4.4 | 1.8 | 4.2 | 2.1 | 2.6 | 0.3 | 1.3 | 2.2 | 2.8 | 3.1 | 1.2 | 1.8 | 2.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 3.3 | 3.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 4.3 | 4.3 | 4.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 9.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 |
Nucleus | -1.7 | -6.1 | -7.7 | -7.7 | -7.8 | -9.8 | -10.2 | -9.8 | -7.9 | -2.3 | -3.3 | -3.3 | -9.7 | -9.7 | -10.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 3.3 | 3.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 |
Vacuole | 1.8 | 1.7 | 1.7 | 1.6 | 0.1 | 0.1 | -0.2 | -1.3 | 1.2 | 0.7 | 2.1 | 0.8 | 7.3 | 7.0 | 6.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5244 | 1.4034 | 1.5028 | 2.209 | 1.8838 | 1.3267 | 0.7586 | 0.5539 | 0.4859 | 0.4058 | -0.0258 | 0.4148 | 0.5026 | 0.0453 | -0.1586 | 0.0752 | 0.6304 | -0.0897 |
Actin | 0.0164 | 0.002 | 0.0018 | 0.0218 | 0.0031 | 0.0083 | 0.0001 | 0.0008 | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0.0071 | 0.0006 | 0.0007 | 0.0114 | 0.0075 | 0.0004 |
Bud | 0.001 | 0.0014 | 0.0031 | 0.0046 | 0.0012 | 0.0028 | 0.0001 | 0.0007 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0004 | 0.0007 | 0.0025 | 0.0034 | 0.0144 | 0.0009 |
Bud Neck | 0.0053 | 0.0009 | 0.0015 | 0.0048 | 0.002 | 0.0101 | 0.0005 | 0.001 | 0.0007 | 0.0009 | 0.0008 | 0.0031 | 0.0038 | 0.0007 | 0.0048 | 0.0106 | 0.0019 | 0.0077 |
Bud Periphery | 0.0017 | 0.0008 | 0.0022 | 0.0091 | 0.0024 | 0.0029 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0003 | 0.0006 | 0.002 | 0.0014 | 0.0043 | 0.0006 |
Bud Site | 0.0062 | 0.0035 | 0.0039 | 0.021 | 0.003 | 0.0017 | 0.0003 | 0.0052 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0057 | 0.0079 | 0.0035 | 0.0013 | 0.0014 | 0.0005 |
Cell Periphery | 0.0005 | 0.0001 | 0.0001 | 0.0014 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 |
Cytoplasm | 0.0846 | 0.1429 | 0.083 | 0.0726 | 0.1039 | 0.0646 | 0.0949 | 0.1316 | 0.0685 | 0.0802 | 0.0677 | 0.1054 | 0.112 | 0.2163 | 0.1421 | 0.061 | 0.2495 | 0.1314 |
Cytoplasmic Foci | 0.0342 | 0.006 | 0.0135 | 0.0159 | 0.0731 | 0.0075 | 0.0026 | 0.004 | 0.0022 | 0.0031 | 0.0015 | 0.003 | 0.0473 | 0.0041 | 0.0087 | 0.0067 | 0.0598 | 0.0027 |
Eisosomes | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0081 | 0.0036 | 0.0022 | 0.0036 | 0.0043 | 0.0019 | 0.001 | 0.003 | 0.0009 | 0.0014 | 0.0009 | 0.001 | 0.0054 | 0.0056 | 0.003 | 0.0013 | 0.0067 | 0.0017 |
Endosome | 0.0661 | 0.0048 | 0.0175 | 0.0325 | 0.1062 | 0.012 | 0.0011 | 0.0022 | 0.001 | 0.0036 | 0.0012 | 0.0015 | 0.0359 | 0.0077 | 0.0176 | 0.0053 | 0.018 | 0.0028 |
Golgi | 0.0142 | 0.0003 | 0.0048 | 0.0092 | 0.0223 | 0.0034 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0054 | 0.0021 | 0.0027 | 0.008 | 0.0132 | 0.0001 |
Lipid Particles | 0.0299 | 0.0004 | 0.0143 | 0.0107 | 0.0168 | 0.0045 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0168 | 0.0002 | 0.0042 | 0.0018 | 0.0442 | 0.0001 |
Mitochondria | 0.0062 | 0.0069 | 0.0063 | 0.0279 | 0.0337 | 0.0088 | 0.0003 | 0.0008 | 0.0006 | 0.0008 | 0.0003 | 0.0005 | 0.001 | 0.0034 | 0.0085 | 0.1097 | 0.0041 | 0.0004 |
None | 0.1751 | 0.3065 | 0.2424 | 0.2425 | 0.225 | 0.1675 | 0.186 | 0.2906 | 0.2139 | 0.2051 | 0.1683 | 0.1679 | 0.1365 | 0.1769 | 0.124 | 0.1656 | 0.1752 | 0.0612 |
Nuclear Periphery | 0.0386 | 0.0172 | 0.0188 | 0.0136 | 0.0268 | 0.0153 | 0.0068 | 0.0115 | 0.0101 | 0.0123 | 0.0119 | 0.0074 | 0.0144 | 0.0205 | 0.0136 | 0.0108 | 0.0167 | 0.0094 |
Nucleolus | 0.0126 | 0.0066 | 0.0124 | 0.0145 | 0.0034 | 0.011 | 0.0037 | 0.0064 | 0.0051 | 0.0085 | 0.008 | 0.0066 | 0.0095 | 0.0023 | 0.004 | 0.0037 | 0.0027 | 0.0062 |
Nucleus | 0.3866 | 0.4315 | 0.5069 | 0.3678 | 0.2871 | 0.5739 | 0.6563 | 0.4601 | 0.6391 | 0.5655 | 0.6379 | 0.6017 | 0.5686 | 0.5313 | 0.6347 | 0.5348 | 0.226 | 0.7313 |
Peroxisomes | 0.0185 | 0.0007 | 0.0077 | 0.0091 | 0.0199 | 0.0022 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.007 | 0.0009 | 0.0041 | 0.004 | 0.044 | 0.0001 |
Punctate Nuclear | 0.072 | 0.0611 | 0.0507 | 0.0668 | 0.0437 | 0.0952 | 0.0441 | 0.0789 | 0.0554 | 0.1145 | 0.1006 | 0.0999 | 0.016 | 0.0138 | 0.0161 | 0.0566 | 0.1045 | 0.0411 |
Vacuole | 0.0157 | 0.0022 | 0.0056 | 0.0405 | 0.0176 | 0.004 | 0.0017 | 0.0016 | 0.0006 | 0.0026 | 0.0004 | 0.0006 | 0.0055 | 0.0037 | 0.0021 | 0.0016 | 0.0046 | 0.0012 |
Vacuole Periphery | 0.0062 | 0.0004 | 0.0012 | 0.0098 | 0.0041 | 0.0021 | 0.0001 | 0.0003 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0015 | 0.0008 | 0.0007 | 0.0007 | 0.0014 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.4168 | 10.0744 | 15.6309 | 15.5554 | 29.2233 | 10.2395 | 16.4369 | 10.3125 | 11.908 | 29.5219 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1039 | 921 | 2919 | 1802 | 1447 | 1991 | 1742 | 884 | 2486 | 2912 | 4661 | 2686 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 715.73 | 906.28 | 1047.32 | 957.62 | 752.34 | 843.81 | 1060.23 | 925.34 | 737.04 | 863.57 | 1052.14 | 947.00 |
Standard Deviation | 109.47 | 153.34 | 214.06 | 165.40 | 112.14 | 132.83 | 191.10 | 129.63 | 112.49 | 142.63 | 205.87 | 155.28 |
Intensity Change Log 2 | — | 0.340541 | 0.549215 | 0.420038 | — | 0.165533 | 0.494921 | 0.298599 | — | 0.253508 | 0.521646 | 0.359082 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000313 | 0.000962 | 0.000473 | 0.001456 | 0.000304 | 0.000276 | 0.000610 | 0.002851 | 0.000307 | 0.000493 | 0.000524 | 0.001915 |
Bud Neck | 0.004444 | 0.010427 | 0.019862 | 0.021903 | 0.003659 | 0.013212 | 0.008872 | 0.017396 | 0.003987 | 0.012331 | 0.015755 | 0.020420 |
Bud Site | 0.002348 | 0.005847 | 0.006548 | 0.022636 | 0.001264 | 0.004864 | 0.001739 | 0.029511 | 0.001717 | 0.005175 | 0.004751 | 0.024898 |
Cell Periphery | 0.000205 | 0.000164 | 0.000171 | 0.000194 | 0.000120 | 0.000161 | 0.000119 | 0.000189 | 0.000156 | 0.000162 | 0.000152 | 0.000192 |
Cytoplasm | 0.129650* | 0.086984 | 0.050954 | 0.112835* | 0.064015 | 0.116999* | 0.019205 | 0.118837* | 0.091447 | 0.107506* | 0.039088 | 0.114810* |
Cytoplasmic Foci | 0.015359 | 0.006974 | 0.000492 | 0.006196 | 0.004639 | 0.007434 | 0.000324 | 0.005735 | 0.009119 | 0.007289 | 0.000429 | 0.006044 |
Eisosomes | 0.000033 | 0.000031 | 0.000013 | 0.000030 | 0.000021 | 0.000020 | 0.000015 | 0.000037 | 0.000026 | 0.000023 | 0.000014 | 0.000032 |
Endoplasmic Reticulum | 0.003283 | 0.007730 | 0.003053 | 0.008258 | 0.003556 | 0.003592 | 0.003926 | 0.007316 | 0.003442 | 0.004901 | 0.003379 | 0.007948 |
Endosome | 0.001014 | 0.001967 | 0.000511 | 0.004807 | 0.000627 | 0.001821 | 0.000570 | 0.004883 | 0.000789 | 0.001867 | 0.000533 | 0.004832 |
Golgi | 0.000412 | 0.001369 | 0.000190 | 0.002208 | 0.000328 | 0.000748 | 0.000148 | 0.001034 | 0.000363 | 0.000944 | 0.000174 | 0.001822 |
Lipid Particles | 0.001940 | 0.001990 | 0.000121 | 0.001007 | 0.000924 | 0.001290 | 0.000140 | 0.000827 | 0.001348 | 0.001511 | 0.000128 | 0.000948 |
Mitochondria | 0.003953 | 0.002390 | 0.003390 | 0.004727 | 0.001487 | 0.006271 | 0.001416 | 0.002007 | 0.002518 | 0.005043 | 0.002652 | 0.003831 |
Mitotic Spindle | 0.003303 | 0.014256 | 0.006897 | 0.070401 | 0.002110 | 0.014167 | 0.003080 | 0.114124* | 0.002608 | 0.014195 | 0.005470 | 0.084791 |
None | 0.015699 | 0.009338 | 0.008912 | 0.006900 | 0.007788 | 0.010447 | 0.005101 | 0.012987 | 0.011094 | 0.010096 | 0.007488 | 0.008903 |
Nuclear Periphery | 0.001095 | 0.002098 | 0.002016 | 0.004885 | 0.001136 | 0.002768 | 0.001006 | 0.002366 | 0.001119 | 0.002556 | 0.001639 | 0.004056 |
Nuclear Periphery Foci | 0.000974 | 0.002657 | 0.000293 | 0.002440 | 0.000904 | 0.000487 | 0.000203 | 0.002530 | 0.000933 | 0.001173 | 0.000260 | 0.002469 |
Nucleolus | 0.005360 | 0.005656 | 0.000930 | 0.002558 | 0.003754 | 0.004933 | 0.001285 | 0.003009 | 0.004425 | 0.005162 | 0.001063 | 0.002706 |
Nucleus | 0.804520* | 0.825503* | 0.889919* | 0.692137* | 0.899849* | 0.797615* | 0.949009* | 0.646068* | 0.860007* | 0.806435* | 0.912003* | 0.676975* |
Peroxisomes | 0.001030 | 0.002607 | 0.000229 | 0.001414 | 0.000451 | 0.001452 | 0.000076 | 0.002175 | 0.000693 | 0.001817 | 0.000172 | 0.001665 |
Vacuole | 0.004618 | 0.010192 | 0.004646 | 0.031359 | 0.002830 | 0.010587 | 0.003073 | 0.025110 | 0.003577 | 0.010462 | 0.004058 | 0.029303 |
Vacuole Periphery | 0.000446 | 0.000858 | 0.000379 | 0.001651 | 0.000235 | 0.000858 | 0.000084 | 0.001008 | 0.000323 | 0.000858 | 0.000268 | 0.001439 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.25 | -4.79 | -7.69 | -1.77 | -5.55 | 0.38 | -8.08 | -4.67 | -4.73 | -3.65 | -4.35 | -8.37 | -8.19 | -6.74 | -6.07 |
Bud Neck | -5.25 | -13.89 | -16.08 | -11.06 | -4.61 | -8.35 | -9.27 | -12.39 | -8.14 | -8.19 | -9.78 | -17.10 | -20.76 | -13.23 | -8.81 |
Bud Site | -2.71 | -3.68 | -8.82 | -5.54 | -6.73 | -5.13 | -3.30 | -9.39 | -7.87 | -8.99 | -5.22 | -5.48 | -13.44 | -10.21 | -11.11 |
Cell Periphery | 1.44 | 2.08 | 2.02 | 0.54 | -0.27 | -1.50 | 0.20 | -3.74 | -0.78 | -5.08 | -0.28 | 1.00 | -0.87 | -0.32 | -2.73 |
Cytoplasm | 5.23 | 13.01 | 6.18 | 0.23 | -10.74 | -9.94 | 13.13 | -1.90 | 6.84 | -12.19 | -3.49 | 16.14 | 2.23 | 6.09 | -16.03 |
Cytoplasmic Foci | 4.44 | 9.03 | 7.28 | 3.93 | -4.84 | -3.17 | 6.74 | -0.32 | 2.49 | -5.46 | 1.58 | 11.00 | 6.23 | 5.28 | -7.21 |
Eisosomes | 0.05 | 8.48 | -3.96 | -3.66 | -17.87 | 0.58 | 1.24 | -12.92 | -13.41 | -13.87 | 1.35 | 7.56 | -13.70 | -15.27 | -21.77 |
Endoplasmic Reticulum | -9.26 | -2.20 | -16.06 | -2.71 | -15.33 | -0.21 | -3.95 | -12.90 | -12.73 | -11.40 | -6.14 | -3.64 | -20.56 | -14.21 | -19.05 |
Endosome | -3.65 | 4.31 | -5.81 | -1.73 | -8.79 | -5.49 | 0.66 | -7.00 | -4.57 | -7.00 | -6.25 | 3.50 | -9.68 | -4.79 | -11.08 |
Golgi | -2.22 | 2.46 | -1.36 | 1.16 | -2.63 | -1.10 | 0.84 | -1.38 | 0.09 | -3.51 | -2.02 | 1.61 | -1.80 | 0.53 | -3.59 |
Lipid Particles | -0.08 | 8.38 | 4.22 | 2.36 | -9.54 | -1.89 | 7.75 | -5.26 | -2.55 | -10.97 | -0.74 | 11.18 | 0.61 | 1.21 | -14.32 |
Mitochondria | 1.43 | 0.61 | -0.26 | -3.86 | -2.00 | -5.58 | -0.43 | -2.29 | 4.88 | -4.18 | -3.64 | -0.34 | -2.23 | 2.57 | -3.27 |
Mitotic Spindle | -3.73 | -2.22 | -13.03 | -9.14 | -12.39 | -5.54 | -2.50 | -14.01 | -12.36 | -13.49 | -6.60 | -3.67 | -19.12 | -15.33 | -18.13 |
None | 3.07 | 4.79 | 4.72 | 1.20 | -0.47 | -1.68 | 2.83 | -1.68 | -0.02 | -5.02 | 1.10 | 4.89 | 2.74 | 1.63 | -3.79 |
Nuclear Periphery | -5.31 | -8.29 | -15.05 | -11.08 | -10.72 | -7.32 | -2.26 | -15.35 | -6.61 | -14.64 | -8.78 | -8.81 | -19.29 | -11.51 | -14.99 |
Nuclear Periphery Foci | -2.34 | 4.52 | -5.16 | -1.70 | -6.85 | 1.50 | 2.73 | -6.61 | -7.06 | -7.31 | -0.78 | 4.43 | -8.32 | -7.74 | -9.99 |
Nucleolus | -0.08 | 5.20 | -3.60 | -3.51 | -8.46 | -2.13 | 2.54 | -7.55 | -6.72 | -8.25 | -1.23 | 5.93 | -8.47 | -7.59 | -11.75 |
Nucleus | -1.35 | -10.29 | 9.56 | 10.74 | 24.76 | 13.66 | -7.18 | 25.41 | 16.46 | 29.66 | 8.54 | -10.13 | 26.12 | 17.96 | 37.60 |
Peroxisomes | -1.87 | 5.38 | 0.55 | 2.01 | -3.10 | -3.12 | 4.08 | -4.02 | -1.41 | -4.91 | -3.20 | 6.26 | -2.76 | 1.21 | -5.56 |
Vacuole | -5.00 | -2.75 | -17.17 | -12.10 | -16.51 | -7.49 | -2.79 | -14.65 | -12.77 | -14.21 | -8.77 | -4.89 | -22.68 | -18.25 | -21.63 |
Vacuole Periphery | -1.79 | 1.35 | -1.32 | 0.84 | -2.52 | -3.58 | 2.71 | -1.78 | 1.83 | -3.12 | -3.80 | 1.63 | -2.55 | 1.61 | -3.64 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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