ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.91 0.96 0.95 0.97 0.96 0.88 0.79 0.78 0.69 0.67 0.46 0.57 0.99 0.99 0.98 0.91 0.95 0.93 0.68 0.84 0.62 0.92 0.9 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0 0 0.07 0.08 0.26 0.39 0.34 0.43 0.54 0.72 0.63 0 0 0 0 0 0 0.17 0.05 0.24 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 1 3 0 1 2
Bud 0 0 2 0 1 2 6 7 9 12 4 2 0 0 0 0 0 0 1 2 4 0 1 4
Bud Neck 0 1 1 0 2 0 1 1 1 2 0 2 0 1 1 0 0 0 2 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 1 7 2 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 2 0 2 1 1 2 0 0 0 0 1 1 2 2 6 5 6 0 0 2 0 1 1
Cytoplasm 172 195 161 155 192 199 247 203 157 195 92 141 276 370 339 125 98 106 137 179 109 114 228 242
Endoplasmic Reticulum 1 0 2 0 0 0 0 0 0 0 0 2 0 2 2 6 5 3 2 1 0 0 0 0
Endosome 0 0 2 1 3 3 2 1 0 0 0 0 1 2 4 2 0 0 5 4 2 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 1 2 3 0 0 0
Mitochondria 34 7 2 11 15 58 123 89 98 157 145 158 1 1 2 6 2 1 35 11 42 2 6 7
Nucleus 0 1 0 0 0 1 0 0 2 0 0 2 1 0 0 2 1 1 0 0 0 0 0 0
Nuclear Periphery 1 2 0 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 4 0 2 3 5 6 3 8 1 0 1 1 0 0 4 2 2 0 1 4
Vac/Vac Membrane 0 0 0 0 0 0 2 0 2 1 2 5 0 0 2 7 2 5 3 2 0 0 2 0
Unique Cell Count 189 203 169 159 199 225 313 260 226 290 201 249 280 375 346 137 103 114 202 213 178 124 254 272
Labelled Cell Count 208 209 171 171 218 265 385 305 275 375 255 324 281 378 354 157 114 124 202 213 178 124 254 272


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 3.8 3.9 3.6 3.8 3.3 3.0 3.0 2.8 2.8 2.5 2.5 4.8 5.1 5.5 7.3 6.6 6.8 5.0 5.1 5.3
Std Deviation (1e-4) 0.5 0.8 1.5 1.8 1.4 1.7 1.5 1.4 1.2 1.0 1.3 1.0 1.0 1.1 8.4 2.2 1.8 1.8 1.2 1.5 1.3
Intensity Change (Log2) – – – -0.1 -0.05 -0.26 -0.36 -0.35 -0.48 -0.5 -0.62 -0.61 0.31 0.38 0.5 0.9 0.77 0.81 0.35 0.39 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8209 1.7452 1.7479 1.0695 1.4835 1.934 -0.7963 -0.3752 -0.4423 -0.8984 -0.1527 -0.448 -1.5372 -1.0037 -1.3373 -0.9787 -1.4376 -1.1302
Actin 0.0354 0.0001 0.0006 0.0224 0.0057 0.0038 0.0217 0.0037 0.0124 0.0029 0.0219 0.0059 0.0261 0.0002 0.0128 0.0005 0.0082 0.0054
Bud 0.0004 0.0001 0.0002 0.0193 0.0101 0.0003 0.0017 0.0035 0.0006 0.001 0.0014 0.0005 0.0056 0.0029 0.0002 0.0265 0.0004 0.0011
Bud Neck 0.0017 0 0.0001 0.0002 0.0008 0.0008 0.0013 0.0002 0.0003 0.0002 0.0006 0.0003 0.0009 0.0001 0.0041 0.0002 0.0004 0.0004
Bud Periphery 0.0005 0.0001 0.0002 0.0166 0.0356 0.0003 0.0029 0.007 0.0007 0.0014 0.0017 0.0026 0.0039 0.001 0.0003 0.0141 0.0006 0.0024
Bud Site 0.0024 0.0002 0.001 0.0037 0.0009 0.0001 0.0049 0.0058 0.0032 0.0003 0.002 0.0001 0.0033 0.0047 0.0045 0.0009 0.0013 0.0002
Cell Periphery 0.0004 0.0001 0.0001 0.0003 0.0008 0.0001 0.0004 0.0004 0.0001 0.0002 0.0003 0.0001 0.0003 0.0002 0.0001 0.0002 0.0001 0.0001
Cytoplasm 0.261 0.4242 0.4147 0.0642 0.3935 0.4156 0.1741 0.3486 0.3416 0.2894 0.2862 0.4471 0.2419 0.293 0.3127 0.2864 0.3005 0.3646
Cytoplasmic Foci 0.0081 0.0039 0.0061 0.0172 0.0098 0.0049 0.0496 0.0085 0.0107 0.0431 0.0394 0.0114 0.0132 0.0052 0.0113 0.0173 0.0112 0.0135
Eisosomes 0.0002 0 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001 0 0.0001 0.0001
Endoplasmic Reticulum 0.0145 0.0009 0.0028 0.0007 0.0044 0.0018 0.0057 0.0028 0.002 0.0117 0.0035 0.0018 0.0018 0.0007 0.0017 0.0019 0.0016 0.0015
Endosome 0.01 0.0011 0.0016 0.0015 0.015 0.0019 0.0382 0.0036 0.0361 0.0282 0.0292 0.0072 0.0038 0.0009 0.0074 0.0037 0.0169 0.0053
Golgi 0.0016 0.0001 0.0002 0.0023 0.0011 0.0003 0.0139 0.0004 0.0069 0.0032 0.0293 0.0017 0.0028 0.0001 0.0018 0.0002 0.0097 0.0008
Lipid Particles 0.0009 0.0001 0.0003 0.003 0.0011 0.0004 0.0267 0.0004 0.0132 0.0036 0.012 0.0047 0.0038 0.0004 0.0016 0.0005 0.0091 0.0013
Mitochondria 0.0014 0.0002 0.0002 0.0011 0.0031 0.0003 0.0089 0.001 0.018 0.0005 0.0115 0.0027 0.0014 0.0001 0.0017 0.0006 0.0054 0.001
None 0.5889 0.5672 0.5688 0.8065 0.4894 0.5418 0.6014 0.6077 0.5409 0.5886 0.4781 0.5087 0.6781 0.6878 0.6337 0.6379 0.5993 0.5849
Nuclear Periphery 0.0297 0.0002 0.0006 0.0006 0.0103 0.0152 0.0029 0.0008 0.0007 0.0043 0.0096 0.0008 0.001 0.0003 0.0009 0.0015 0.0068 0.0079
Nucleolus 0.0004 0.0001 0.0001 0.0005 0.0008 0.0004 0.0008 0.0003 0.0001 0.0003 0.0005 0.0001 0.0002 0.0001 0.0001 0.0003 0.0028 0.0002
Nucleus 0.0356 0.0005 0.0008 0.0033 0.0094 0.0035 0.0057 0.0014 0.0011 0.0027 0.0103 0.0014 0.0011 0.001 0.0012 0.0022 0.0072 0.0047
Peroxisomes 0.0006 0 0.0002 0.0315 0.0008 0.0002 0.0182 0.0003 0.0068 0.0023 0.0031 0.0004 0.0069 0.0001 0.0018 0.0004 0.0016 0.0015
Punctate Nuclear 0.0023 0.0002 0.0005 0.0026 0.0042 0.0074 0.0067 0.0008 0.0007 0.0124 0.0157 0.0007 0.002 0.0006 0.0007 0.0028 0.0142 0.0019
Vacuole 0.0027 0.0006 0.0006 0.0019 0.0023 0.0004 0.012 0.0024 0.0027 0.003 0.0396 0.0013 0.0013 0.0007 0.001 0.0015 0.0014 0.0007
Vacuole Periphery 0.0012 0.0001 0.0001 0.0003 0.001 0.0003 0.0021 0.0003 0.001 0.0007 0.0041 0.0005 0.0003 0.0001 0.0003 0.0003 0.0011 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.0712 7.3065 10.8435 14.3189 7.6535 28.1371 12.7231 13.5733 14.0257 13.4224
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
177 145 427 1362 1246 1826 466 1069 1423 1971 893 2431

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 684.36 676.71 879.30 838.24 674.21 704.18 973.39 792.82 675.47 702.16 928.40 818.27
Standard Deviation 74.22 98.81 103.41 119.54 80.40 103.80 120.25 138.76 79.73 103.69 121.94 130.31
Intensity Change Log 2 — -0.016218 0.361600 0.292608 — 0.062746 0.529820 0.233795 — 0.023509 0.447533 0.263721

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000073 0.000340 0.000376 0.000616 0.000050 0.000563 0.000269 0.000900 0.000053 0.000547 0.000320 0.000741
Bud Neck 0.016414 0.049847 0.003506 0.008291 0.003570 0.035930 0.001541 0.006880 0.005168 0.036954 0.002480 0.007671
Bud Site 0.002241 0.016231 0.004626 0.038755 0.001385 0.015850 0.006777 0.045831 0.001492 0.015878 0.005748 0.041866
Cell Periphery 0.000135 0.000160 0.000136 0.000167 0.000143 0.000198 0.000167 0.000591 0.000142 0.000195 0.000152 0.000354
Cytoplasm 0.385144* 0.288869* 0.325145* 0.472420* 0.620004* 0.417152* 0.354570* 0.532549* 0.590791* 0.407714* 0.340500* 0.498861*
Cytoplasmic Foci 0.304579* 0.350026* 0.009311 0.032699 0.144380 0.243089* 0.025952 0.025474 0.164307 0.250956* 0.017995 0.029522
Eisosomes 0.000167 0.000186 0.000049 0.000051 0.000072 0.000172 0.000088 0.001881 0.000084 0.000173 0.000069 0.000856
Endoplasmic Reticulum 0.000351 0.000482 0.003909 0.002148 0.000545 0.000495 0.003399 0.001726 0.000521 0.000494 0.003643 0.001962
Endosome 0.009221 0.041772 0.002837 0.010957 0.004004 0.020824 0.005885 0.007573 0.004653 0.022365 0.004428 0.009469
Golgi 0.002760 0.008061 0.000259 0.008003 0.000704 0.005091 0.000139 0.002125 0.000960 0.005310 0.000196 0.005418
Lipid Particles 0.013865 0.022286 0.001715 0.001713 0.004297 0.007690 0.003060 0.002629 0.005487 0.008763 0.002417 0.002116
Mitochondria 0.003561 0.005722 0.001613 0.006475 0.001088 0.014114 0.000442 0.002858 0.001396 0.013496 0.001002 0.004885
Mitotic Spindle 0.004402 0.001233 0.004967 0.063762 0.000154 0.008418 0.003737 0.026898 0.000682 0.007889 0.004325 0.047551
None 0.001018 0.001226 0.003761 0.003373 0.006974 0.003207 0.004177 0.006113 0.006233 0.003061 0.003978 0.004578
Nuclear Periphery 0.000615 0.001144 0.001921 0.001598 0.000293 0.000440 0.001866 0.000973 0.000333 0.000492 0.001892 0.001323
Nuclear Periphery Foci 0.000231 0.000581 0.001384 0.001604 0.000399 0.000253 0.002497 0.001354 0.000378 0.000277 0.001965 0.001494
Nucleolus 0.001308 0.002551 0.000481 0.002113 0.000824 0.001715 0.000482 0.000321 0.000884 0.001776 0.000481 0.001325
Nucleus 0.201691* 0.104499 0.562908* 0.162301* 0.189850* 0.140262* 0.430895* 0.212568* 0.191323* 0.137631* 0.494018* 0.184405*
Peroxisomes 0.010072 0.043706 0.000251 0.002122 0.001262 0.015234 0.000891 0.010744 0.002358 0.017328 0.000585 0.005913
Vacuole 0.041593 0.059625 0.070634 0.175837 0.019551 0.067545 0.152976 0.109008 0.022293 0.066962 0.113603 0.146450
Vacuole Periphery 0.000558 0.001455 0.000212 0.004994 0.000450 0.001760 0.000189 0.001004 0.000464 0.001738 0.000200 0.003240

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.75 -3.51 -7.42 -0.98 -0.15 -1.98 -11.07 -3.37 -0.71 -2.01 -2.06 -6.87 -5.27 -0.17 -1.90
Bud Neck -2.85 2.83 2.40 3.99 -1.79 -15.97 2.03 -2.63 14.97 -5.64 -15.14 2.32 -0.87 15.41 -4.43
Bud Site -3.42 -1.83 -7.73 -0.69 -6.09 -10.29 -2.61 -7.36 -2.62 -5.28 -10.75 -3.56 -10.93 -2.58 -7.80
Cell Periphery -0.81 -0.71 1.55 1.84 2.87 -3.15 -1.38 -1.96 -1.25 -1.76 -3.26 -1.21 -1.44 0.03 -1.12
Cytoplasm 3.31 4.54 -0.10 -4.32 -7.12 22.17 26.12 17.37 -1.08 -10.20 20.36 29.41 21.53 0.58 -12.01
Cytoplasmic Foci -1.90 17.27 16.51 17.51 -7.58 -14.41 26.24 27.02 41.63 -0.17 -12.66 31.58 30.42 44.85 -4.48
Eisosomes -0.65 5.68 6.16 7.05 1.11 -10.76 -4.89 -1.88 -1.62 -1.81 -9.88 0.19 -1.76 -1.24 -1.77
Endoplasmic Reticulum 0.37 -10.80 -8.98 -14.20 6.62 0.69 -15.88 -13.52 -13.87 8.02 0.75 -18.66 -18.75 -19.44 10.12
Endosome -4.58 5.47 3.10 5.15 -3.38 -14.04 -1.53 0.08 12.23 1.18 -14.39 0.85 -0.24 13.90 -0.99
Golgi -1.49 1.31 -0.04 1.69 -3.29 -6.82 6.89 -0.50 5.65 -2.10 -6.42 3.18 -1.88 4.24 -3.84
Lipid Particles -1.23 2.88 2.96 3.61 1.33 -5.15 2.65 3.43 7.83 1.89 -3.70 4.48 5.39 10.39 2.55
Mitochondria -1.31 1.48 0.48 2.72 -1.78 -8.99 4.64 -0.98 8.26 -2.53 -8.92 1.71 -1.80 8.04 -2.90
Mitotic Spindle 0.79 -0.65 -5.48 -9.48 -5.89 -5.66 -2.03 -6.00 -2.96 -4.96 -4.88 -2.66 -10.98 -7.38 -7.97
None -0.82 -4.82 -5.56 -4.77 0.08 4.18 5.22 2.99 -1.58 -2.51 3.89 4.83 3.81 -0.95 -1.76
Nuclear Periphery -0.79 -9.83 -10.11 -2.55 -1.46 -3.38 -20.51 -8.42 -7.43 3.34 -2.52 -24.40 -16.24 -13.85 0.76
Nuclear Periphery Foci -1.71 -5.68 -8.02 -4.32 0.93 3.01 -10.00 -4.22 -4.69 3.53 2.19 -10.77 -7.97 -8.55 3.43
Nucleolus -1.25 2.35 0.60 1.48 -1.05 -3.76 2.72 3.50 7.20 2.71 -3.91 3.07 0.82 3.78 -0.87
Nucleus 4.88 -17.07 -0.25 -7.43 22.90 7.69 -16.41 -9.17 -15.50 9.82 8.69 -26.71 -6.47 -15.97 22.91
Peroxisomes -3.58 3.95 3.66 4.72 -3.11 -12.63 2.66 -2.09 5.41 -2.56 -11.80 5.30 -0.26 10.42 -2.99
Vacuole -0.86 -5.52 -22.64 -24.58 -15.97 -18.03 -18.72 -24.72 -17.31 2.60 -16.21 -20.16 -41.95 -32.11 -8.25
Vacuole Periphery -3.19 4.19 -2.10 -0.57 -2.71 -9.22 6.83 -0.05 6.70 -2.16 -9.59 7.21 -2.20 1.41 -3.06
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (49%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YJR149W

YJR149W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yjr149w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available