ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.91 0.96 0.86 1.0 0.99 0.96 0.9 0.89 0.81 0.85 0.75 0.76 0.99 0.99 1.0 – 0.93 0.96 0.66 0.91 0.7 0.8 0.84 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.07 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.15 0.1 0.05 0 0 0.06 0.19 0.14 0.27 0.23 0.46 0.36 0 0 0 – 0 0 0.2 0 0.16 0.08 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – 1 0 1 0 1 0 0 2
Bud 1 1 1 0 0 0 1 5 9 3 2 5 0 0 0 – 0 0 0 0 0 0 3 3
Bud Neck 2 0 0 7 18 5 10 13 12 3 1 6 2 1 0 – 1 1 0 0 0 0 0 3
Bud Site 0 0 0 0 0 0 0 1 1 1 0 3 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 8 2 1 2 0 1 3 1 0 3 3 2 – 6 5 0 1 0 1 3 2
Cytoplasm 179 75 19 224 284 232 289 286 230 246 165 168 161 172 171 – 139 206 143 80 51 90 197 204
Endoplasmic Reticulum 2 0 0 0 1 0 0 1 1 0 1 1 1 0 0 – 11 15 1 0 0 2 1 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 1 2 6 0 1 0 1 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 5 0 0 0 0 3
Mitochondria 29 8 1 0 0 14 61 46 78 66 100 81 1 0 2 – 3 7 43 3 11 9 12 9
Nucleus 0 0 0 1 0 2 1 1 0 0 1 1 0 0 2 – 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 1 4 4 6 7 3 6 0 0 0 – 2 0 2 0 0 0 0 2
Vac/Vac Membrane 0 1 0 0 0 0 1 0 1 0 0 1 0 0 0 – 5 4 5 0 0 2 6 3
Unique Cell Count 196 78 22 224 286 242 322 322 285 288 219 222 162 173 171 149 214 218 88 74 114 236 249
Labelled Cell Count 214 85 23 240 306 255 369 357 339 329 274 272 169 176 177 170 241 218 88 74 114 236 249


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 3.7 4.1 4.1 4.3 3.5 3.3 3.8 3.1 3.4 2.8 3.1 4.4 4.6 4.5 4.8 5.9 5.0 4.2 4.7 5.2
Std Deviation (1e-4) 0.7 0.6 1.3 0.8 1.2 1.7 1.5 1.4 1.1 1.1 0.8 0.8 0.9 1.1 0.8 0.8 2.5 1.0 1.4 1.4 2.1
Intensity Change (Log2) – – – -0.02 0.05 -0.24 -0.32 -0.13 -0.43 -0.3 -0.54 -0.41 0.08 0.16 0.12 0.23 0.51 0.26 0.01 0.18 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6245 0.9424 0.6714 1.1065 0.0339 0.6708 -1.2281 -0.1665 -0.5707 -0.6795 -0.8087 -0.5027 -1.2577 -0.6513 -1.0163 -1.0848 -0.2279 -1.0265
Actin 0.0163 0.0165 0.0051 0.0308 0.0044 0.0005 0.0033 0.0003 0.0013 0.0002 0.0002 0.0009 0.0156 0.0037 0.0103 0.0017 0.031 0.0012
Bud 0.0008 0.0007 0.0011 0.0052 0.0003 0.0015 0.0001 0.0002 0.0004 0 0 0.0005 0.0004 0.0005 0.0009 0.0021 0.0013 0.0005
Bud Neck 0.0004 0.0002 0.0015 0.0045 0.0002 0.0024 0.0001 0.0008 0.0001 0.0001 0.0001 0.0005 0.0047 0.0004 0.0015 0.0004 0.0007 0.0005
Bud Periphery 0.0021 0.0006 0.0014 0.0081 0.0011 0.0023 0.0002 0.0002 0.0008 0 0 0.0004 0.0016 0.0003 0.0018 0.0033 0.0022 0.0004
Bud Site 0.0025 0.011 0.0035 0.0062 0.0002 0.0025 0.0007 0.0144 0.0007 0 0 0 0.0025 0.003 0.0047 0.0044 0.0023 0.0001
Cell Periphery 0.0002 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0 0 0 0.0004 0.0002 0.0002 0.0004 0.0002 0.0002
Cytoplasm 0.0297 0.0903 0.0551 0.0403 0.1102 0.0978 0.1034 0.1842 0.1251 0.0973 0.1491 0.145 0.1208 0.2343 0.1792 0.229 0.185 0.2611
Cytoplasmic Foci 0.0209 0.0068 0.0056 0.0234 0.0052 0.0087 0.0066 0.0063 0.0047 0.0028 0.0046 0.0087 0.032 0.0113 0.0168 0.0139 0.0596 0.0115
Eisosomes 0.0003 0.0003 0.0002 0.0003 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0 0.0001 0.0004 0.0001 0.0002 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0023 0.0022 0.0006 0.0025 0.001 0.0003 0.0011 0.0007 0.0012 0.0004 0.0011 0.0005 0.0035 0.002 0.0014 0.0017 0.0015 0.0018
Endosome 0.0082 0.0031 0.0046 0.0133 0.0068 0.001 0.0019 0.001 0.0009 0.0004 0.0018 0.0009 0.0156 0.0083 0.0093 0.0284 0.0707 0.0052
Golgi 0.0048 0.0006 0.0029 0.0105 0.004 0.0002 0.0005 0.0001 0.0001 0 0.0001 0.0001 0.01 0.0018 0.0041 0.0053 0.0513 0.0003
Lipid Particles 0.0086 0.0014 0.009 0.0113 0.0062 0.0005 0.0009 0.0002 0.0003 0.0001 0.0001 0.0001 0.0109 0.0029 0.0093 0.0125 0.0389 0.0011
Mitochondria 0.0149 0.0026 0.0022 0.0118 0.0142 0.0004 0.0006 0.0004 0.0006 0.0002 0.0001 0.0003 0.0161 0.0007 0.0053 0.0241 0.0488 0.0013
None 0.8483 0.8529 0.8945 0.7823 0.8372 0.8744 0.873 0.7858 0.8588 0.8966 0.8388 0.8379 0.75 0.7061 0.7382 0.6547 0.453 0.686
Nuclear Periphery 0.0043 0.0019 0.0006 0.0037 0.0019 0.0005 0.0019 0.0004 0.0008 0.0003 0.001 0.0004 0.001 0.0022 0.0017 0.0014 0.0021 0.0037
Nucleolus 0.0008 0.0002 0.0003 0.0011 0.0002 0.0006 0.0004 0.0004 0.0003 0.0001 0.0001 0.0001 0.0004 0.0132 0.0016 0.0022 0.0017 0.0182
Nucleus 0.0024 0.001 0.0014 0.0034 0.0014 0.0028 0.0021 0.0016 0.0013 0.0005 0.0012 0.0011 0.0007 0.0026 0.003 0.0015 0.0015 0.0019
Peroxisomes 0.0219 0.0048 0.0082 0.0319 0.0013 0.0009 0.0004 0.0002 0.0005 0.0001 0.0001 0.0002 0.0095 0.0023 0.0068 0.0053 0.0364 0.001
Punctate Nuclear 0.0085 0.0015 0.0013 0.0071 0.001 0.0022 0.0016 0.0019 0.0014 0.0006 0.0011 0.0019 0.0018 0.0027 0.0013 0.0011 0.0032 0.0016
Vacuole 0.0009 0.0008 0.0005 0.0013 0.0016 0.0004 0.0005 0.0005 0.0005 0.0002 0.0003 0.0002 0.0015 0.001 0.0019 0.0041 0.0044 0.0014
Vacuole Periphery 0.0007 0.0003 0.0002 0.0007 0.0014 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0005 0.0002 0.0006 0.0024 0.0041 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.7698 2.8426 0.7031 2.8568 4.1228 7.4155 2.8741 2.5865 2.0252 4.4518
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1788 764 2171 64 1602 2180 636 1179 3390 2944 2807 1243

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 653.89 631.99 727.15 848.14 612.85 656.07 780.06 761.07 634.50 649.82 739.14 765.55
Standard Deviation 72.19 90.46 92.16 98.12 71.21 79.76 104.18 83.07 74.60 83.34 97.56 86.09
Intensity Change Log 2 — -0.049146 0.153205 0.375254 — 0.098316 0.348051 0.312495 — 0.024079 0.250760 0.345232

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000343 0.000357 0.000100 0.000306 0.000076 0.000179 0.001572 0.000091 0.000217 0.000225 0.000434 0.000102
Bud Neck 0.023441 0.031923 0.011613 0.002671 0.009002 0.034554 0.010939 0.004581 0.016618 0.033872 0.011461 0.004482
Bud Site 0.004809 0.011861 0.027018 0.005270 0.001559 0.008813 0.131056* 0.004519 0.003273 0.009604 0.050591 0.004557
Cell Periphery 0.000147 0.000137 0.000141 0.000276 0.000118 0.000119 0.000351 0.000142 0.000133 0.000124 0.000189 0.000149
Cytoplasm 0.435161* 0.374413* 0.527247* 0.314620* 0.513062* 0.463891* 0.438800* 0.511697* 0.471975* 0.440671* 0.507207* 0.501549*
Cytoplasmic Foci 0.254916* 0.266274* 0.016080 0.058458 0.199690* 0.214572* 0.033267 0.041782 0.228818* 0.227989* 0.019974 0.042640
Eisosomes 0.000214 0.000150 0.000024 0.000138 0.000133 0.000149 0.000061 0.000052 0.000176 0.000149 0.000032 0.000056
Endoplasmic Reticulum 0.000609 0.000733 0.000925 0.003093 0.000550 0.000390 0.002140 0.000902 0.000581 0.000479 0.001200 0.001014
Endosome 0.013084 0.034615 0.002279 0.006305 0.007649 0.013139 0.010565 0.004561 0.010515 0.018712 0.004157 0.004651
Golgi 0.002885 0.004655 0.000204 0.000125 0.001360 0.002628 0.000871 0.000342 0.002164 0.003154 0.000355 0.000331
Lipid Particles 0.012875 0.010250 0.000812 0.004108 0.009463 0.006099 0.002259 0.001617 0.011262 0.007176 0.001140 0.001745
Mitochondria 0.008869 0.012198 0.001247 0.000480 0.002758 0.004584 0.002730 0.001153 0.005981 0.006560 0.001583 0.001118
Mitotic Spindle 0.001602 0.001676 0.013547 0.001710 0.000857 0.004374 0.004005 0.002818 0.001250 0.003674 0.011385 0.002761
None 0.002509 0.006284 0.003450 0.001913 0.004141 0.002440 0.003428 0.003637 0.003280 0.003438 0.003445 0.003548
Nuclear Periphery 0.000524 0.000531 0.001740 0.001459 0.000613 0.000372 0.000771 0.000760 0.000566 0.000413 0.001520 0.000796
Nuclear Periphery Foci 0.000215 0.000475 0.000325 0.001823 0.000398 0.000187 0.000611 0.000740 0.000302 0.000262 0.000390 0.000796
Nucleolus 0.001305 0.004020 0.000658 0.000837 0.001429 0.001268 0.000804 0.000849 0.001364 0.001982 0.000691 0.000849
Nucleus 0.178860* 0.137393* 0.348513* 0.403312* 0.213532* 0.180580* 0.218786* 0.361883* 0.195245* 0.169373* 0.319120* 0.364016*
Peroxisomes 0.010638 0.025004 0.000513 0.000830 0.003749 0.010306 0.004309 0.000446 0.007382 0.014121 0.001373 0.000465
Vacuole 0.046140 0.074252 0.042416 0.191998 0.029280 0.050258 0.131539 0.056690 0.038173 0.056485 0.062609 0.063657
Vacuole Periphery 0.000853 0.002798 0.001147 0.000268 0.000584 0.001096 0.001137 0.000740 0.000726 0.001538 0.001145 0.000716

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.10 1.67 -0.52 -0.66 -3.04 -3.87 -3.03 -1.39 2.80 2.98 -0.11 -1.61 1.27 4.12 2.80
Bud Neck -2.42 6.98 10.48 8.58 4.93 -11.97 -0.45 3.68 13.49 3.00 -9.16 4.76 9.68 15.29 5.37
Bud Site -5.22 -8.04 -0.67 1.34 4.15 -8.93 -11.01 -3.40 3.79 10.69 -8.60 -13.59 -1.51 4.75 12.75
Cell Periphery 0.98 1.46 -2.03 -2.34 -2.51 -0.06 -5.79 -1.98 -1.86 5.02 1.48 -3.35 -0.97 -1.95 2.06
Cytoplasm 5.20 -6.68 8.39 5.42 10.83 5.79 6.91 2.31 -2.65 -4.92 4.76 -0.61 -0.48 -3.92 0.01
Cytoplasmic Foci -1.21 45.66 24.04 20.61 -2.69 -2.28 30.58 30.23 35.91 -2.73 0.05 56.36 46.52 42.96 -10.25
Eisosomes 5.35 18.69 4.15 0.74 -7.34 -1.82 9.16 11.00 15.03 1.85 3.41 22.46 17.44 17.29 -9.18
Endoplasmic Reticulum -0.93 -2.98 -4.90 -4.77 -4.35 2.23 -6.60 -5.59 -7.84 4.46 1.47 -5.90 -6.78 -8.20 0.40
Endosome -7.86 12.22 6.30 10.62 -4.15 -4.78 -1.07 3.60 7.68 3.16 -7.25 9.79 7.34 12.20 -0.70
Golgi -2.37 9.09 9.30 7.04 1.26 -2.28 1.34 2.62 5.73 1.44 -2.31 7.43 7.06 8.11 0.12
Lipid Particles 2.22 13.90 8.95 6.05 -5.36 3.64 7.74 9.77 8.76 1.21 5.64 16.81 15.74 11.90 -3.65
Mitochondria -1.43 6.44 6.99 6.55 3.12 -2.49 0.54 2.50 6.34 1.71 -0.56 6.39 6.46 8.39 1.24
Mitotic Spindle -0.13 -6.56 -0.87 -0.79 4.81 -3.15 -1.72 -2.14 0.90 0.29 -2.85 -7.00 -1.92 0.48 5.08
None -2.58 -1.67 0.12 2.20 0.82 3.25 1.31 1.41 -2.13 -0.40 -0.23 0.18 -0.03 0.20 -0.20
Nuclear Periphery -0.11 -19.80 -7.18 -7.05 -0.59 3.34 -4.24 -4.31 -10.78 1.72 3.19 -19.18 -8.02 -11.83 12.70
Nuclear Periphery Foci -4.26 -1.02 -5.49 -4.90 -5.15 2.81 -3.51 -3.22 -5.42 1.64 0.79 -2.30 -5.36 -5.78 -2.70
Nucleolus -4.14 2.33 2.60 5.94 0.86 0.70 2.51 2.72 3.09 0.19 -2.44 3.59 2.87 5.99 -0.55
Nucleus 4.91 -26.22 -5.07 -6.29 1.51 5.38 -0.91 -16.17 -20.92 -10.50 5.49 -22.95 -20.16 -23.09 -2.04
Peroxisomes -5.32 11.74 10.71 9.56 -0.81 -6.45 0.18 8.05 10.63 2.77 -6.18 10.99 13.90 14.31 2.48
Vacuole -6.22 -2.75 -8.74 -7.86 -8.51 -10.05 -15.50 -12.26 -6.94 8.98 -9.12 -13.93 -12.08 -7.83 -2.77
Vacuole Periphery -6.18 -0.38 7.70 8.28 2.87 -2.33 -2.31 -0.14 1.13 0.86 -4.44 -1.14 0.34 2.42 0.88
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (12%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YJR154W

YJR154W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yjr154w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available