Standard name
Human Ortholog
Description Alpha subunit of farnesyltransferase and geranylgeranyltransferase-I; farnesyltransferase (Ram2p-Ram1p heterodimer) catalyzes the addition of 15-carbon isoprenoid farnesyl to substrate proteins containing a CAAX consensus motif; type I geranylgeranyltransferase (Ram2p-Cdc43p heterodimer) catalyzes the addition of the 20-carbon isoprenoid geranylgeranyl to substrate proteins containing a CaaL consensus motif; required for membrane localization of Ras proteins and a-factor
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.97 0.98 0.97 0.95 0.9 0.81 0.84 0.72 0.73 0.7 0.71 0.97 0.98 0.97 0.92 0.97 0.95 0.7 0.86 0.8 0.87 0.85 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.06 0 0.06 0 0.12 0.29 0.18 0.34 0.39 0.48 0.38 0 0 0 0 0 0 0.09 0 0.08 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.14 0.07 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 2 2 2 0 3 3
Bud 1 1 0 0 0 0 0 3 4 2 0 2 0 0 0 0 1 0 1 1 0 2 4 5
Bud Neck 1 0 4 3 1 0 0 5 0 0 0 1 1 1 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 2 2 2 3 1 0 0 0 0 0 2 0 8 3 4 0 0 0 1 2 1
Cytoplasm 101 133 169 188 261 202 242 265 140 147 112 96 180 295 284 217 226 215 76 116 129 123 207 212
Endoplasmic Reticulum 1 0 1 0 0 0 1 2 4 0 0 0 1 1 2 15 14 9 1 0 0 1 0 0
Endosome 0 0 0 0 3 0 1 1 1 0 0 1 0 1 1 0 1 1 5 1 1 1 3 0
Golgi 0 0 0 0 2 1 0 2 0 0 0 0 0 1 4 0 1 0 1 1 0 0 3 2
Mitochondria 15 8 0 11 8 28 87 56 66 79 77 51 3 3 2 3 4 5 10 5 13 4 6 10
Nucleus 0 0 3 0 2 2 4 5 2 4 6 0 1 0 1 1 0 1 0 0 1 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 2 0 0 0 1 2 2 4 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 3 0 0 0 0 0 1 2 1 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 4 2 0 3 0 0 0 0 0 0 0 0 0 0 1 0 3 5
Vac/Vac Membrane 0 1 0 0 3 3 14 7 7 4 0 2 1 0 1 32 17 15 3 1 0 0 1 3
Unique Cell Count 108 137 172 194 274 225 300 314 194 201 161 136 185 301 294 236 232 227 109 136 161 141 244 252
Labelled Cell Count 119 144 177 204 285 239 356 353 226 242 197 157 187 306 297 277 267 250 109 136 161 141 244 252


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.7 4.6 4.7 5.2 4.4 4.2 4.9 4.2 4.2 4.0 4.5 4.9 5.3 5.5 6.9 6.6 6.6 4.8 4.9 5.4
Std Deviation (1e-4) 0.7 1.3 0.9 1.5 2.2 1.7 1.8 2.1 1.9 1.7 2.0 2.0 1.0 1.5 1.9 1.8 1.4 2.1 1.6 1.5 2.1
Intensity Change (Log2) – – – 0.03 0.18 -0.05 -0.12 0.09 -0.14 -0.13 -0.19 -0.03 0.09 0.22 0.27 0.58 0.52 0.54 0.06 0.1 0.24

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.0 3.6 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5047 -0.9088 -0.729 -0.8698 -0.5034 -1.1053 -0.8847 -0.6105 -0.4151 -0.6186 -0.6436 -0.7468 -1.2418 -1.0444 -1.2252 -1.0294 -0.9637 -0.9392
Actin 0.0442 0.0039 0.0448 0.0013 0.0122 0.0031 0.0258 0.0008 0.0066 0.0601 0.0434 0.0156 0.0084 0.0014 0.0034 0.0022 0.0194 0.0049
Bud 0.008 0.0025 0.0063 0.0003 0.0099 0.0009 0.0066 0.0073 0.0026 0.0097 0.0007 0.0119 0.0031 0.0052 0.0015 0.009 0.0028 0.0043
Bud Neck 0.0171 0.0065 0.0153 0.0002 0.0009 0.0006 0.0021 0.0006 0.0028 0.001 0.0605 0.0019 0.0006 0.0003 0.0005 0.0007 0.0018 0.0038
Bud Periphery 0.0088 0.0071 0.0048 0.0007 0.0287 0.0009 0.0031 0.0262 0.0062 0.0366 0.0009 0.0263 0.0093 0.0034 0.0021 0.0123 0.0042 0.0015
Bud Site 0.0194 0.0098 0.0205 0.0003 0.0081 0.0002 0.0137 0.0242 0.026 0.0172 0.0034 0.0039 0.0069 0.0128 0.0098 0.0064 0.0272 0.0026
Cell Periphery 0.0012 0.0006 0.0019 0.0001 0.0008 0.0001 0.0006 0.0003 0.0007 0.0004 0.0002 0.0012 0.0006 0.0007 0.0003 0.0001 0.0002 0.0002
Cytoplasm 0.0435 0.0392 0.0445 0.0376 0.0943 0.112 0.0349 0.0739 0.0604 0.0745 0.0165 0.0889 0.1081 0.1623 0.1342 0.1884 0.1235 0.2401
Cytoplasmic Foci 0.027 0.016 0.03 0.0051 0.0463 0.0122 0.0285 0.0077 0.0273 0.016 0.0281 0.0212 0.0162 0.015 0.0071 0.024 0.0076 0.0164
Eisosomes 0.001 0.0002 0.0011 0.0002 0.0003 0.0001 0.0005 0.0002 0.0003 0.0003 0.0003 0.0003 0.0006 0.0003 0.0005 0.0002 0.0005 0.0002
Endoplasmic Reticulum 0.0048 0.0011 0.0024 0.0034 0.0008 0.0006 0.0009 0.0003 0.0009 0.001 0.001 0.0018 0.0019 0.0011 0.0012 0.0011 0.0011 0.0015
Endosome 0.0242 0.0048 0.025 0.0356 0.0257 0.0034 0.0105 0.0009 0.0172 0.0332 0.06 0.0098 0.0056 0.001 0.0022 0.0017 0.0043 0.0027
Golgi 0.0072 0.0006 0.01 0.0028 0.0095 0.003 0.0064 0.0003 0.0125 0.0073 0.0595 0.0033 0.0014 0.0003 0.0009 0.0005 0.0048 0.0007
Lipid Particles 0.0183 0.0029 0.0113 0.002 0.0239 0.0056 0.0101 0.0001 0.0118 0.0043 0.0311 0.005 0.0036 0.0041 0.0017 0.001 0.0129 0.0013
Mitochondria 0.0095 0.0027 0.0413 0.0649 0.0469 0.0058 0.0087 0.0012 0.0322 0.0056 0.0109 0.0189 0.0048 0.0033 0.0014 0.0013 0.0126 0.0011
None 0.7219 0.866 0.6814 0.8025 0.592 0.8259 0.8179 0.8451 0.7518 0.7152 0.6525 0.7612 0.8074 0.7663 0.819 0.7304 0.759 0.7012
Nuclear Periphery 0.0077 0.0048 0.0057 0.0185 0.0155 0.0034 0.0015 0.0003 0.0009 0.0008 0.0009 0.0016 0.0028 0.0009 0.0007 0.001 0.0045 0.0013
Nucleolus 0.0031 0.0013 0.0035 0.0007 0.0063 0.0005 0.0012 0.0007 0.0029 0.0003 0.0003 0.0008 0.0009 0.0053 0.0029 0.001 0.0005 0.0013
Nucleus 0.0046 0.0065 0.0054 0.0096 0.0033 0.0018 0.0025 0.0022 0.0043 0.0009 0.0005 0.005 0.003 0.0024 0.0039 0.0036 0.0011 0.0058
Peroxisomes 0.0168 0.0018 0.0249 0.0015 0.041 0.0157 0.016 0.0007 0.0192 0.012 0.0246 0.012 0.0084 0.0105 0.0012 0.0043 0.007 0.003
Punctate Nuclear 0.0062 0.0108 0.0147 0.0041 0.0225 0.0032 0.0051 0.0062 0.0077 0.0011 0.0017 0.0062 0.0041 0.0017 0.0042 0.0097 0.0031 0.0049
Vacuole 0.0041 0.0085 0.0038 0.0039 0.0079 0.0005 0.0033 0.0005 0.0047 0.002 0.002 0.0025 0.0016 0.0012 0.001 0.0009 0.0009 0.001
Vacuole Periphery 0.0012 0.0024 0.0017 0.0048 0.0031 0.0005 0.0005 0.0001 0.001 0.0005 0.0011 0.0008 0.0006 0.0004 0.0002 0.0002 0.0011 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.968 33.1904 26.168 31.6886 33.0951 41.7461 36.9671 35.0365 33.2524 37.3195
Translational Efficiency 0.8919 0.8848 1.013 0.7295 0.7333 0.7901 0.8298 0.8238 0.9193 0.6461

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
15 429 1992 114 1148 74 2181 102 1163 503 4173 216

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 693.83 741.32 896.51 898.25 657.88 754.93 889.18 943.66 658.34 743.32 892.68 919.69
Standard Deviation 66.12 93.81 114.82 138.53 77.64 119.63 103.96 108.05 77.61 98.16 109.34 127.10
Intensity Change Log 2 — 0.095514 0.369737 0.372535 — 0.198518 0.434651 0.520443 — 0.146566 0.401696 0.446417

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000065 0.013746 0.003503 0.004658 0.001309 0.009928 0.000350 0.012740 0.001293 0.013184 0.001855 0.008475
Bud Neck 0.012225 0.015650 0.017814 0.010917 0.015181 0.009360 0.011825 0.004291 0.015142 0.014725 0.014684 0.007788
Bud Site 0.069268 0.041998 0.032443 0.014550 0.014666 0.019258 0.020601 0.048624 0.015370 0.038652 0.026254 0.030641
Cell Periphery 0.000270 0.000940 0.000238 0.000261 0.000714 0.000449 0.000195 0.001439 0.000708 0.000868 0.000216 0.000817
Cytoplasm 0.337543 0.323612 0.304819 0.369839 0.443481 0.274084 0.401851 0.321662 0.442115 0.316325 0.355532 0.347089
Cytoplasmic Foci 0.207725 0.200465 0.006770 0.040345 0.164768 0.316175 0.012013 0.032653 0.165322 0.217488 0.009510 0.036713
Eisosomes 0.000175 0.000186 0.000033 0.000088 0.000342 0.000193 0.000042 0.000154 0.000340 0.000187 0.000037 0.000119
Endoplasmic Reticulum 0.001521 0.002253 0.004731 0.004050 0.001662 0.010201 0.002247 0.006026 0.001660 0.003422 0.003433 0.004983
Endosome 0.007411 0.016028 0.002885 0.036278 0.007862 0.027385 0.003973 0.029497 0.007856 0.017699 0.003454 0.033076
Golgi 0.000991 0.014843 0.001936 0.005208 0.005749 0.006150 0.000268 0.069180 0.005688 0.013564 0.001064 0.035417
Lipid Particles 0.032198 0.009139 0.000920 0.003242 0.009476 0.006110 0.001297 0.012302 0.009769 0.008694 0.001117 0.007520
Mitochondria 0.000622 0.041066 0.005871 0.003016 0.027893 0.018389 0.004755 0.011287 0.027542 0.037730 0.005287 0.006922
Mitotic Spindle 0.003272 0.040110 0.023658 0.058246 0.006129 0.003284 0.016697 0.007573 0.006092 0.034693 0.020020 0.034317
None 0.001010 0.005102 0.005737 0.003529 0.008059 0.001182 0.005801 0.003140 0.007968 0.004526 0.005771 0.003345
Nuclear Periphery 0.001229 0.000536 0.002289 0.001521 0.000903 0.000430 0.001891 0.001569 0.000907 0.000521 0.002081 0.001543
Nuclear Periphery Foci 0.000465 0.000975 0.000550 0.001536 0.000956 0.000550 0.000430 0.004096 0.000949 0.000912 0.000487 0.002745
Nucleolus 0.000446 0.003017 0.000611 0.000621 0.001283 0.000781 0.000604 0.000534 0.001273 0.002688 0.000607 0.000580
Nucleus 0.282279 0.198706 0.532467 0.208703 0.242106 0.199750 0.436451 0.267447 0.242624 0.198860 0.482285 0.236443
Peroxisomes 0.005919 0.024340 0.002356 0.013438 0.011802 0.019800 0.002153 0.022239 0.011726 0.023672 0.002250 0.017594
Vacuole 0.034678 0.043157 0.049891 0.193604 0.033179 0.071860 0.075956 0.140244 0.033199 0.047380 0.063514 0.168406
Vacuole Periphery 0.000688 0.004130 0.000479 0.026351 0.002480 0.004679 0.000599 0.003303 0.002457 0.004211 0.000542 0.015467

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.81 -3.41 -1.84 2.19 -0.17 -0.92 2.22 -0.74 0.69 -1.14 -2.70 0.06 -1.08 2.23 -1.12
Bud Neck -0.42 -0.72 0.64 3.06 4.63 1.61 2.31 1.96 0.04 0.56 0.32 0.70 2.93 2.75 3.29
Bud Site 0.50 0.77 1.03 4.55 4.96 -0.58 0.10 -1.04 -0.48 -1.08 -3.81 -1.86 -0.32 2.96 0.65
Cell Periphery -2.43 0.54 0.56 2.85 0.11 1.66 6.84 1.51 0.10 -1.56 -0.57 6.52 3.44 2.35 -1.53
Cytoplasm -0.01 1.03 0.90 2.18 -0.16 6.35 12.34 10.12 1.99 4.88 8.97 17.14 10.80 3.82 2.95
Cytoplasmic Foci 0.51 3.93 3.58 16.59 -4.46 -5.58 31.46 26.20 11.32 -2.57 -4.86 32.21 26.10 20.32 -5.05
Eisosomes -0.30 1.98 0.88 3.95 -6.83 2.35 4.94 3.65 3.03 -4.58 2.50 5.03 3.80 4.38 -7.74
Endoplasmic Reticulum -0.89 -4.16 -3.66 -3.85 -1.10 -0.99 -6.21 -4.91 0.50 -2.77 -1.40 -10.39 -6.19 -2.09 -2.49
Endosome -1.98 1.62 -1.00 0.40 -2.39 -2.37 7.48 -0.15 2.15 -1.46 -4.19 8.50 -1.00 1.73 -2.78
Golgi -3.84 0.45 -0.22 3.70 -0.64 -0.17 5.91 -0.93 -0.87 -1.54 -2.49 5.43 -0.43 1.05 -1.62
Lipid Particles 0.75 1.06 0.98 3.32 -4.50 3.13 11.09 2.13 0.67 -1.59 0.52 10.66 4.50 2.68 -2.58
Mitochondria -5.71 -7.00 -1.46 5.58 4.68 0.97 7.39 6.89 1.46 0.13 -1.25 7.23 7.59 5.65 2.00
Mitotic Spindle -3.44 -2.34 -1.94 0.31 -0.93 1.10 -3.73 -2.49 -2.64 -1.95 -4.60 -6.18 -3.37 -0.38 -2.10
None -3.47 -9.28 -2.46 0.49 0.48 4.97 2.34 1.51 -2.26 -0.13 1.96 2.26 1.97 0.04 0.21
Nuclear Periphery 0.85 -3.43 -2.91 -5.22 -0.50 2.63 -11.01 -6.00 -8.44 0.36 2.17 -12.55 -6.28 -8.02 -0.37
Nuclear Periphery Foci -1.42 -0.93 -2.47 -2.09 -2.35 0.15 1.12 -1.69 -1.73 -1.94 -0.11 0.91 -2.61 -2.63 -3.02
Nucleolus -3.06 -0.22 -0.98 2.47 -0.95 1.88 3.73 2.95 1.14 -0.45 -1.96 3.80 2.04 2.73 -1.04
Nucleus 0.74 -4.00 0.31 -1.22 13.01 1.45 -24.88 -1.82 -2.39 6.73 3.29 -31.74 -1.01 -2.93 13.63
Peroxisomes 0.17 1.06 0.93 4.17 -1.33 -1.26 5.99 1.56 1.90 -0.85 -2.97 6.09 2.49 4.47 -1.45
Vacuole -1.16 -3.86 -10.01 -10.48 -9.15 -2.87 -18.14 -9.76 -8.00 -6.94 -3.55 -19.86 -14.83 -13.59 -11.37
Vacuole Periphery -3.06 0.60 -1.42 -0.75 -1.49 -1.18 4.12 2.13 2.11 -0.71 -1.61 4.20 -0.75 -0.16 -1.55
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Alpha subunit of farnesyltransferase and geranylgeranyltransferase-I; farnesyltransferase (Ram2p-Ram1p heterodimer) catalyzes the addition of 15-carbon isoprenoid farnesyl to substrate proteins containing a CAAX consensus motif; type I geranylgeranyltransferase (Ram2p-Cdc43p heterodimer) catalyzes the addition of the 20-carbon isoprenoid geranylgeranyl to substrate proteins containing a CaaL consensus motif; required for membrane localization of Ras proteins and a-factor
Localization
Cell Percentages cytoplasm (22%), nucleus (11%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ram2

Ram2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ram2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available