Standard name
Human Ortholog
Description ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; SPT23 has a paralog, MGA2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0.09 0.05 0 0
Bud 0 0 0 0 0 0 0 0 – 0.05 0 0.06 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0.05 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0.05 0 – – 0 0 0 0.06 0.08 0.07 0.05
Cytoplasm 0.94 0.93 0.87 0.84 0.89 0.75 0.62 0.56 – 0.51 0.44 0.46 0.94 1.0 0.99 – – 0.97 0.58 0.45 0.21 0.38 0.51 0.58
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 – – 0.06 0.22 0.22 0.37 0.19 0.14 0.1
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.22 0.09 0.4 0.46 0.56 – 0.69 0.68 0.68 0 0 0 – – 0 0.08 0.11 0.16 0.21 0.14 0.13
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 – – 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 1 0 0 0 0 0 – 0 1 1 1 0 0 – – 4 11 5 29 13 9 9
Bud 2 7 3 8 2 8 10 18 – 21 8 20 1 0 0 – – 2 0 1 2 1 3 3
Bud Neck 3 2 0 0 0 0 0 0 – 0 0 0 1 1 0 – – 2 9 9 8 8 6 10
Bud Site 0 0 0 0 0 1 0 2 – 2 2 0 0 0 0 – – 0 – – – – – –
Cell Periphery 7 6 11 10 5 4 6 2 – 0 3 7 11 11 10 – – 5 5 8 18 22 19 21
Cytoplasm 276 176 234 194 243 236 262 256 – 222 136 164 161 225 270 – – 251 193 94 67 115 135 231
Endoplasmic Reticulum 12 2 9 3 2 3 3 3 – 2 6 3 8 1 0 – – 16 72 45 116 58 36 38
Endosome 1 1 6 1 5 4 6 7 – 1 2 3 0 0 0 – – 1 1 6 4 1 3 3
Golgi 2 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 1 2 0 0 1
Mitochondria 4 8 16 50 25 127 195 260 – 301 209 242 0 0 0 – – 8 26 23 50 63 36 51
Nucleus 0 0 0 1 1 1 2 2 – 2 2 0 0 0 1 – – 1 0 1 0 0 0 1
Nuclear Periphery 0 1 0 0 1 0 0 1 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 1 1 1 – 0 1 0 0 0 0 – – 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 1 1 2 1 – 2 0 1 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 4 3 – 4 7 4 1 0 1 – – 0 0 0 1 1 1 2
Vac/Vac Membrane 1 1 2 5 7 8 19 4 – 8 8 7 0 0 0 – – 1 6 10 8 12 8 16
Unique Cell Count 294 189 269 232 272 314 421 461 435 308 354 172 226 272 260 333 212 318 303 266 396
Labelled Cell Count 309 206 283 273 292 394 510 560 565 385 452 184 238 282 291 333 212 318 303 266 396


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.0 4.5 4.6 4.7 3.6 3.4 3.4 – 2.8 3.0 3.1 5.5 5.5 5.5 4.0 4.3 5.3 5.9 6.1 6.2
Std Deviation (1e-4) 0.5 1.2 1.7 2.1 2.4 1.7 1.3 1.0 – 0.8 0.7 0.9 0.6 0.6 0.7 1.0 0.9 1.4 1.3 1.8 1.6
Intensity Change (Log2) – – – 0.03 0.06 -0.31 -0.4 -0.4 – -0.67 -0.6 -0.54 0.29 0.3 0.28 -0.16 -0.05 0.23 0.4 0.45 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Cell Periphery 0 0 0 0 0 – 0 0 0 1.1 0.4 -0.2 – – -1.5
Cytoplasm -1.1 0.8 -3.6 -7.1 -8.7 – -9.7 -10.7 -10.5 2.2 5.4 5.7 – – 4.0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0.7 0 0 – – 1.5
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Mitochondria 0 0 0 0 0 – 0 0 0 -3.3 -3.7 -4.1 – – -1.6
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Vacuole 0 0 0 2.8 0 – 0 0 0 0 0 0 – – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.4817 0.0044 0.0061 0.0006 0.0129 0.0135 – – – – – – – – – – – –
Bud 0.0035 0.0005 0.0007 0.0009 0.0001 0.0021 – – – – – – – – – – – –
Bud Neck 0.0116 0.0002 0.0018 0.0023 0.0006 0.011 – – – – – – – – – – – –
Bud Periphery 0.0076 0.0005 0.0004 0.0005 0.0002 0.0028 – – – – – – – – – – – –
Bud Site 0.0028 0.0078 0.0202 0.0087 0.0002 0.0102 – – – – – – – – – – – –
Cell Periphery 0.0012 0.001 0.0004 0.0003 0.0004 0.0026 – – – – – – – – – – – –
Cytoplasm 0.0085 0.5898 0.322 0.3315 0.5634 0.5496 – – – – – – – – – – – –
Cytoplasmic Foci 0.0039 0.0133 0.0663 0.1401 0.0117 0.0236 – – – – – – – – – – – –
Eisosomes 0.0036 0.0002 0.0001 0 0.0003 0.0005 – – – – – – – – – – – –
Endoplasmic Reticulum 0.0556 0.0228 0.0127 0.019 0.0337 0.0194 – – – – – – – – – – – –
Endosome 0.0896 0.004 0.2306 0.2314 0.0395 0.0261 – – – – – – – – – – – –
Golgi 0.0249 0.0008 0.0233 0.0324 0.0057 0.0035 – – – – – – – – – – – –
Lipid Particles 0.0052 0.0015 0.0663 0.0031 0.0025 0.0169 – – – – – – – – – – – –
Mitochondria 0.0176 0.0004 0.0033 0.003 0.0053 0.0167 – – – – – – – – – – – –
None 0.0191 0.3438 0.1434 0.0305 0.1278 0.1656 – – – – – – – – – – – –
Nuclear Periphery 0.2122 0.0028 0.005 0.003 0.1739 0.0023 – – – – – – – – – – – –
Nucleolus 0.0012 0.0002 0.0057 0.0012 0.0005 0.0723 – – – – – – – – – – – –
Nucleus 0.0216 0.0018 0.0193 0.0024 0.0078 0.0233 – – – – – – – – – – – –
Peroxisomes 0.0012 0.0002 0.0106 0.0054 0.0001 0.0055 – – – – – – – – – – – –
Punctate Nuclear 0.0049 0.0015 0.0203 0.001 0.0034 0.0114 – – – – – – – – – – – –
Vacuole 0.0067 0.0023 0.0347 0.1618 0.0032 0.0179 – – – – – – – – – – – –
Vacuole Periphery 0.0157 0.0003 0.0068 0.0209 0.0069 0.0032 – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.9618 17.0013 12.6157 19.2374 16.9525 15.953 20.2596 17.7139 19.6285 22.6236
Translational Efficiency 0.7215 0.7508 0.9605 0.6135 0.6373 0.7876 0.583 0.7786 0.6577 0.6146

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1523 1115 141 1457 1072 1722 1799 899 2595 2837 1940 2356

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 824.64 699.26 962.51 940.74 600.97 764.59 881.77 856.46 732.24 738.91 887.64 908.58
Standard Deviation 97.10 98.67 105.81 129.19 563.79 90.92 118.36 127.04 385.97 99.31 119.35 134.74
Intensity Change Log 2 — -0.237935 0.223037 0.190032 — 0.347393 0.553109 0.511093 — 0.038188 0.371478 0.334172

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001225 0.000468 0.001713 0.003513 0.000442 0.000356 0.001324 0.000462 0.000901 0.000400 0.001352 0.002349
Bud Neck 0.010063 0.029198 0.004183 0.013309 0.041456 0.033150 0.010096 0.008247 0.023031 0.031597 0.009666 0.011377
Bud Site 0.024073 0.013002 0.011448 0.055999 0.009626 0.013095 0.027415 0.018749 0.018105 0.013058 0.026255 0.041785
Cell Periphery 0.000691 0.000293 0.001655 0.001499 0.000750 0.000363 0.001177 0.000476 0.000716 0.000336 0.001212 0.001109
Cytoplasm 0.447940* 0.363675* 0.299735* 0.423968* 0.398369* 0.372316* 0.475721* 0.466642* 0.427462* 0.368920* 0.462930* 0.440251*
Cytoplasmic Foci 0.087231 0.299921* 0.130880 0.058215 0.292454* 0.294471* 0.062231 0.118898 0.172009 0.296613* 0.067221 0.081371
Eisosomes 0.000337 0.000333 0.000396 0.000519 0.001704 0.000468 0.000423 0.000254 0.000902 0.000415 0.000421 0.000418
Endoplasmic Reticulum 0.009817 0.004591 0.031721 0.020908 0.005096 0.003527 0.035641 0.010826 0.007866 0.003945 0.035356 0.017061
Endosome 0.009400 0.036097 0.020486 0.007207 0.012080 0.033876 0.006887 0.015824 0.010507 0.034749 0.007876 0.010495
Golgi 0.002330 0.008585 0.000571 0.003104 0.012644 0.009060 0.000731 0.002473 0.006590 0.008873 0.000719 0.002863
Lipid Particles 0.012222 0.024735 0.022352 0.008567 0.039019 0.028455 0.010751 0.014618 0.023292 0.026993 0.011595 0.010876
Mitochondria 0.002298 0.010447 0.000194 0.002220 0.031714 0.012119 0.000500 0.001366 0.014450 0.011462 0.000478 0.001894
Mitotic Spindle 0.008234 0.002581 0.000262 0.012787 0.002161 0.003864 0.000816 0.004960 0.005725 0.003359 0.000775 0.009800
None 0.001283 0.004617 0.000469 0.002460 0.006820 0.002510 0.001016 0.007617 0.003570 0.003338 0.000976 0.004427
Nuclear Periphery 0.002336 0.000977 0.002002 0.001149 0.000581 0.000729 0.001585 0.001951 0.001611 0.000826 0.001615 0.001455
Nuclear Periphery Foci 0.003583 0.001542 0.047378 0.009150 0.000404 0.000482 0.012396 0.007060 0.002270 0.000899 0.014938 0.008352
Nucleolus 0.000430 0.003564 0.000549 0.000219 0.000918 0.001137 0.000241 0.001294 0.000632 0.002091 0.000264 0.000629
Nucleus 0.203917* 0.086247 0.196811* 0.113205 0.102593 0.086945 0.227879* 0.100239 0.162060* 0.086671 0.225621* 0.108257
Peroxisomes 0.003459 0.011858 0.001351 0.002160 0.013526 0.013398 0.001077 0.001914 0.007618 0.012793 0.001097 0.002066
Vacuole 0.167881 0.093446 0.225543* 0.258533* 0.025434 0.086652 0.121869 0.214897* 0.109036 0.089322 0.129404 0.241883*
Vacuole Periphery 0.001250 0.003825 0.000299 0.001310 0.002210 0.003029 0.000223 0.001235 0.001647 0.003342 0.000229 0.001282

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.97 -1.49 -3.49 -6.25 -1.68 0.68 -4.85 -0.07 -0.84 5.05 2.20 -2.23 -3.56 -6.41 -2.06
Bud Neck -7.50 4.20 -0.86 7.11 -5.38 2.40 10.08 10.63 11.92 1.63 -4.25 8.07 8.27 13.44 -0.10
Bud Site 3.41 3.33 -4.99 -8.43 -8.19 -1.77 -6.16 -2.82 -1.57 2.71 2.30 -3.07 -5.86 -8.20 -2.41
Cell Periphery 4.79 -5.16 -3.44 -6.49 1.95 3.99 -4.53 4.22 -0.89 9.28 5.29 -6.27 -2.07 -5.78 2.71
Cytoplasm 7.13 8.56 5.37 -1.88 -5.46 2.75 -6.81 -4.06 -6.69 1.42 7.58 -3.86 1.72 -5.14 5.16
Cytoplasmic Foci -30.45* -3.96 9.68 36.53 7.53 -0.26 34.37 23.37 27.22 -11.28 -23.10 24.53 22.34 48.63 -3.35
Eisosomes -0.19 -1.56 -2.34 -2.16 -0.62 6.27 6.51 7.46 7.21 6.86 5.69 5.57 6.27 1.88 2.03
Endoplasmic Reticulum 4.09 -5.76 -3.94 -7.51 3.77 1.78 -13.54 -2.99 -4.42 10.20 4.83 -13.22 -5.02 -9.06 9.46
Endosome -11.76 -5.70 3.84 13.56 7.63 -12.77 5.20 -1.76 10.16 -6.14 -18.09 3.52 1.40 18.95 -1.98
Golgi -6.39 3.56 0.20 5.81 -2.05 3.13 14.05 8.46 5.82 -1.55 -3.25 12.69 6.33 8.54 -2.40
Lipid Particles -6.72 -3.72 4.35 9.95 5.73 3.78 11.43 9.51 8.17 -2.11 -2.44 9.09 10.44 14.30 2.50
Mitochondria -5.43 4.11 0.92 5.75 -2.24 6.73 11.49 11.13 9.63 -1.84 2.03 11.66 10.41 10.71 -2.34
Mitotic Spindle 2.53 4.44 -1.31 -3.76 -5.49 -1.05 1.18 -1.08 -0.32 -2.32 1.57 4.13 -1.76 -3.39 -5.64
None -3.53 1.90 -1.24 2.61 -3.30 2.78 4.04 -0.00 -2.67 -3.86 0.28 4.01 -0.35 -0.67 -4.46
Nuclear Periphery 3.89 1.03 3.14 -1.26 2.57 -0.83 -5.74 -3.97 -3.89 -1.00 3.91 -0.17 0.27 -4.00 0.56
Nuclear Periphery Foci 4.73 -8.64 -6.17 -10.39 7.64 -0.70 -16.59 -10.79 -10.77 5.73 5.50 -15.36 -10.67 -15.10 7.81
Nucleolus -4.71 -1.31 4.45 5.01 4.42 -1.58 6.17 -0.77 -0.24 -2.39 -5.36 6.96 0.04 4.71 -2.21
Nucleus 19.29 0.54 14.99 -5.09 4.92 3.25 -19.92 0.23 -3.06 20.44 18.28 -11.28 12.15 -6.21 22.39
Peroxisomes -8.51 3.18 2.51 10.35 -0.61 -0.04 13.54 12.17 11.92 -2.63 -6.59 13.27 10.58 15.46 -2.66
Vacuole 13.83 -3.68 -15.83 -28.39 -4.54 -18.57 -27.14 -27.44 -18.82 -12.31 7.10 -4.72 -26.99 -34.70 -22.88
Vacuole Periphery -3.77 2.50 0.53 3.85 -1.19 -2.33 10.06 3.60 5.17 -3.59 -4.76 6.35 1.78 5.70 -2.41
Standard name
Human Ortholog
Description ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; SPT23 has a paralog, MGA2, that arose from the whole genome duplication
Localization
Cell Percentages ER (19%), cytoplasm (8%), mixed (56%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.00023)
Subcompartmental Group N/A

Spt23

Spt23


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spt23-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available