Standard name
Human Ortholog
Description ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; SPT23 has a paralog, MGA2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0.09 0.05 0 0
Bud 0 0 0 0 0 0 0 0 – 0.05 0 0.06 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0.05 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0.05 0 – – 0 0 0 0.06 0.08 0.07 0.05
Cytoplasm 0.94 0.93 0.87 0.84 0.89 0.75 0.62 0.56 – 0.51 0.44 0.46 0.94 1.0 0.99 – – 0.97 0.58 0.45 0.21 0.38 0.51 0.58
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 – – 0.06 0.22 0.22 0.37 0.19 0.14 0.1
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.22 0.09 0.4 0.46 0.56 – 0.69 0.68 0.68 0 0 0 – – 0 0.08 0.11 0.16 0.21 0.14 0.13
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 – – 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 1 0 0 0 0 0 – 0 1 1 1 0 0 – – 4 11 5 29 13 9 9
Bud 2 7 3 8 2 8 10 18 – 21 8 20 1 0 0 – – 2 0 1 2 1 3 3
Bud Neck 3 2 0 0 0 0 0 0 – 0 0 0 1 1 0 – – 2 9 9 8 8 6 10
Bud Site 0 0 0 0 0 1 0 2 – 2 2 0 0 0 0 – – 0 – – – – – –
Cell Periphery 7 6 11 10 5 4 6 2 – 0 3 7 11 11 10 – – 5 5 8 18 22 19 21
Cytoplasm 276 176 234 194 243 236 262 256 – 222 136 164 161 225 270 – – 251 193 94 67 115 135 231
Endoplasmic Reticulum 12 2 9 3 2 3 3 3 – 2 6 3 8 1 0 – – 16 72 45 116 58 36 38
Endosome 1 1 6 1 5 4 6 7 – 1 2 3 0 0 0 – – 1 1 6 4 1 3 3
Golgi 2 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 1 2 0 0 1
Mitochondria 4 8 16 50 25 127 195 260 – 301 209 242 0 0 0 – – 8 26 23 50 63 36 51
Nucleus 0 0 0 1 1 1 2 2 – 2 2 0 0 0 1 – – 1 0 1 0 0 0 1
Nuclear Periphery 0 1 0 0 1 0 0 1 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 1 1 1 – 0 1 0 0 0 0 – – 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 1 1 2 1 – 2 0 1 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 4 3 – 4 7 4 1 0 1 – – 0 0 0 1 1 1 2
Vac/Vac Membrane 1 1 2 5 7 8 19 4 – 8 8 7 0 0 0 – – 1 6 10 8 12 8 16
Unique Cell Count 294 189 269 232 272 314 421 461 435 308 354 172 226 272 260 333 212 318 303 266 396
Labelled Cell Count 309 206 283 273 292 394 510 560 565 385 452 184 238 282 291 333 212 318 303 266 396


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.0 4.5 4.6 4.7 3.6 3.4 3.4 – 2.8 3.0 3.1 5.5 5.5 5.5 4.0 4.3 5.3 5.9 6.1 6.2
Std Deviation (1e-4) 0.5 1.2 1.7 2.1 2.4 1.7 1.3 1.0 – 0.8 0.7 0.9 0.6 0.6 0.7 1.0 0.9 1.4 1.3 1.8 1.6
Intensity Change (Log2) – – – 0.03 0.06 -0.31 -0.4 -0.4 – -0.67 -0.6 -0.54 0.29 0.3 0.28 -0.16 -0.05 0.23 0.4 0.45 0.46

WT3RAP60RAP140RAP220RAP300RAP380RAP540RAP620RAP7000246WT3HU80HU120HU1600246WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30246WT1AF100AF140AF1800246
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Cell Periphery 0 0 0 0 0 – 0 0 0 1.1 0.4 -0.2 – – -1.5
Cytoplasm -1.1 0.8 -3.6 -7.1 -8.7 – -9.7 -10.7 -10.5 2.2 5.4 5.7 – – 4.0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0.7 0 0 – – 1.5
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Mitochondria 0 0 0 0 0 – 0 0 0 -3.3 -3.7 -4.1 – – -1.6
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Vacuole 0 0 0 2.8 0 – 0 0 0 0 0 0 – – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.4817 0.0044 0.0061 0.0006 0.0129 0.0135 – – – – – – – – – – – –
Bud 0.0035 0.0005 0.0007 0.0009 0.0001 0.0021 – – – – – – – – – – – –
Bud Neck 0.0116 0.0002 0.0018 0.0023 0.0006 0.011 – – – – – – – – – – – –
Bud Periphery 0.0076 0.0005 0.0004 0.0005 0.0002 0.0028 – – – – – – – – – – – –
Bud Site 0.0028 0.0078 0.0202 0.0087 0.0002 0.0102 – – – – – – – – – – – –
Cell Periphery 0.0012 0.001 0.0004 0.0003 0.0004 0.0026 – – – – – – – – – – – –
Cytoplasm 0.0085 0.5898 0.322 0.3315 0.5634 0.5496 – – – – – – – – – – – –
Cytoplasmic Foci 0.0039 0.0133 0.0663 0.1401 0.0117 0.0236 – – – – – – – – – – – –
Eisosomes 0.0036 0.0002 0.0001 0 0.0003 0.0005 – – – – – – – – – – – –
Endoplasmic Reticulum 0.0556 0.0228 0.0127 0.019 0.0337 0.0194 – – – – – – – – – – – –
Endosome 0.0896 0.004 0.2306 0.2314 0.0395 0.0261 – – – – – – – – – – – –
Golgi 0.0249 0.0008 0.0233 0.0324 0.0057 0.0035 – – – – – – – – – – – –
Lipid Particles 0.0052 0.0015 0.0663 0.0031 0.0025 0.0169 – – – – – – – – – – – –
Mitochondria 0.0176 0.0004 0.0033 0.003 0.0053 0.0167 – – – – – – – – – – – –
None 0.0191 0.3438 0.1434 0.0305 0.1278 0.1656 – – – – – – – – – – – –
Nuclear Periphery 0.2122 0.0028 0.005 0.003 0.1739 0.0023 – – – – – – – – – – – –
Nucleolus 0.0012 0.0002 0.0057 0.0012 0.0005 0.0723 – – – – – – – – – – – –
Nucleus 0.0216 0.0018 0.0193 0.0024 0.0078 0.0233 – – – – – – – – – – – –
Peroxisomes 0.0012 0.0002 0.0106 0.0054 0.0001 0.0055 – – – – – – – – – – – –
Punctate Nuclear 0.0049 0.0015 0.0203 0.001 0.0034 0.0114 – – – – – – – – – – – –
Vacuole 0.0067 0.0023 0.0347 0.1618 0.0032 0.0179 – – – – – – – – – – – –
Vacuole Periphery 0.0157 0.0003 0.0068 0.0209 0.0069 0.0032 – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.9618 17.0013 12.6157 19.2374 16.9525 15.953 20.2596 17.7139 19.6285 22.6236
Translational Efficiency 0.7215 0.7508 0.9605 0.6135 0.6373 0.7876 0.583 0.7786 0.6577 0.6146

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1523 1115 141 1457 1072 1722 1799 899 2595 2837 1940 2356

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 824.64 699.26 962.51 940.74 600.97 764.59 881.77 856.46 732.24 738.91 887.64 908.58
Standard Deviation 97.10 98.67 105.81 129.19 563.79 90.92 118.36 127.04 385.97 99.31 119.35 134.74
Intensity Change Log 2 — -0.237935 0.223037 0.190032 — 0.347393 0.553109 0.511093 — 0.038188 0.371478 0.334172

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001225 0.000468 0.001713 0.003513 0.000442 0.000356 0.001324 0.000462 0.000901 0.000400 0.001352 0.002349
Bud Neck 0.010063 0.029198 0.004183 0.013309 0.041456 0.033150 0.010096 0.008247 0.023031 0.031597 0.009666 0.011377
Bud Site 0.024073 0.013002 0.011448 0.055999 0.009626 0.013095 0.027415 0.018749 0.018105 0.013058 0.026255 0.041785
Cell Periphery 0.000691 0.000293 0.001655 0.001499 0.000750 0.000363 0.001177 0.000476 0.000716 0.000336 0.001212 0.001109
Cytoplasm 0.447940 0.363675 0.299735 0.423968 0.398369 0.372316 0.475721 0.466642 0.427462 0.368920 0.462930 0.440251
Cytoplasmic Foci 0.087231 0.299921 0.130880 0.058215 0.292454 0.294471 0.062231 0.118898 0.172009 0.296613 0.067221 0.081371
Eisosomes 0.000337 0.000333 0.000396 0.000519 0.001704 0.000468 0.000423 0.000254 0.000902 0.000415 0.000421 0.000418
Endoplasmic Reticulum 0.009817 0.004591 0.031721 0.020908 0.005096 0.003527 0.035641 0.010826 0.007866 0.003945 0.035356 0.017061
Endosome 0.009400 0.036097 0.020486 0.007207 0.012080 0.033876 0.006887 0.015824 0.010507 0.034749 0.007876 0.010495
Golgi 0.002330 0.008585 0.000571 0.003104 0.012644 0.009060 0.000731 0.002473 0.006590 0.008873 0.000719 0.002863
Lipid Particles 0.012222 0.024735 0.022352 0.008567 0.039019 0.028455 0.010751 0.014618 0.023292 0.026993 0.011595 0.010876
Mitochondria 0.002298 0.010447 0.000194 0.002220 0.031714 0.012119 0.000500 0.001366 0.014450 0.011462 0.000478 0.001894
Mitotic Spindle 0.008234 0.002581 0.000262 0.012787 0.002161 0.003864 0.000816 0.004960 0.005725 0.003359 0.000775 0.009800
None 0.001283 0.004617 0.000469 0.002460 0.006820 0.002510 0.001016 0.007617 0.003570 0.003338 0.000976 0.004427
Nuclear Periphery 0.002336 0.000977 0.002002 0.001149 0.000581 0.000729 0.001585 0.001951 0.001611 0.000826 0.001615 0.001455
Nuclear Periphery Foci 0.003583 0.001542 0.047378 0.009150 0.000404 0.000482 0.012396 0.007060 0.002270 0.000899 0.014938 0.008352
Nucleolus 0.000430 0.003564 0.000549 0.000219 0.000918 0.001137 0.000241 0.001294 0.000632 0.002091 0.000264 0.000629
Nucleus 0.203917 0.086247 0.196811 0.113205 0.102593 0.086945 0.227879 0.100239 0.162060 0.086671 0.225621 0.108257
Peroxisomes 0.003459 0.011858 0.001351 0.002160 0.013526 0.013398 0.001077 0.001914 0.007618 0.012793 0.001097 0.002066
Vacuole 0.167881 0.093446 0.225543 0.258533 0.025434 0.086652 0.121869 0.214897 0.109036 0.089322 0.129404 0.241883
Vacuole Periphery 0.001250 0.003825 0.000299 0.001310 0.002210 0.003029 0.000223 0.001235 0.001647 0.003342 0.000229 0.001282

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.97 -1.49 -3.49 -6.25 -1.68 0.68 -4.85 -0.07 -0.84 5.05 2.20 -2.23 -3.56 -6.41 -2.06
Bud Neck -7.50 4.20 -0.86 7.11 -5.38 2.40 10.08 10.63 11.92 1.63 -4.25 8.07 8.27 13.44 -0.10
Bud Site 3.41 3.33 -4.99 -8.43 -8.19 -1.77 -6.16 -2.82 -1.57 2.71 2.30 -3.07 -5.86 -8.20 -2.41
Cell Periphery 4.79 -5.16 -3.44 -6.49 1.95 3.99 -4.53 4.22 -0.89 9.28 5.29 -6.27 -2.07 -5.78 2.71
Cytoplasm 7.13 8.56 5.37 -1.88 -5.46 2.75 -6.81 -4.06 -6.69 1.42 7.58 -3.86 1.72 -5.14 5.16
Cytoplasmic Foci -30.45 -3.96 9.68 36.53 7.53 -0.26 34.37 23.37 27.22 -11.28 -23.10 24.53 22.34 48.63 -3.35
Eisosomes -0.19 -1.56 -2.34 -2.16 -0.62 6.27 6.51 7.46 7.21 6.86 5.69 5.57 6.27 1.88 2.03
Endoplasmic Reticulum 4.09 -5.76 -3.94 -7.51 3.77 1.78 -13.54 -2.99 -4.42 10.20 4.83 -13.22 -5.02 -9.06 9.46
Endosome -11.76 -5.70 3.84 13.56 7.63 -12.77 5.20 -1.76 10.16 -6.14 -18.09 3.52 1.40 18.95 -1.98
Golgi -6.39 3.56 0.20 5.81 -2.05 3.13 14.05 8.46 5.82 -1.55 -3.25 12.69 6.33 8.54 -2.40
Lipid Particles -6.72 -3.72 4.35 9.95 5.73 3.78 11.43 9.51 8.17 -2.11 -2.44 9.09 10.44 14.30 2.50
Mitochondria -5.43 4.11 0.92 5.75 -2.24 6.73 11.49 11.13 9.63 -1.84 2.03 11.66 10.41 10.71 -2.34
Mitotic Spindle 2.53 4.44 -1.31 -3.76 -5.49 -1.05 1.18 -1.08 -0.32 -2.32 1.57 4.13 -1.76 -3.39 -5.64
None -3.53 1.90 -1.24 2.61 -3.30 2.78 4.04 -0.00 -2.67 -3.86 0.28 4.01 -0.35 -0.67 -4.46
Nuclear Periphery 3.89 1.03 3.14 -1.26 2.57 -0.83 -5.74 -3.97 -3.89 -1.00 3.91 -0.17 0.27 -4.00 0.56
Nuclear Periphery Foci 4.73 -8.64 -6.17 -10.39 7.64 -0.70 -16.59 -10.79 -10.77 5.73 5.50 -15.36 -10.67 -15.10 7.81
Nucleolus -4.71 -1.31 4.45 5.01 4.42 -1.58 6.17 -0.77 -0.24 -2.39 -5.36 6.96 0.04 4.71 -2.21
Nucleus 19.29 0.54 14.99 -5.09 4.92 3.25 -19.92 0.23 -3.06 20.44 18.28 -11.28 12.15 -6.21 22.39
Peroxisomes -8.51 3.18 2.51 10.35 -0.61 -0.04 13.54 12.17 11.92 -2.63 -6.59 13.27 10.58 15.46 -2.66
Vacuole 13.83 -3.68 -15.83 -28.39 -4.54 -18.57 -27.14 -27.44 -18.82 -12.31 7.10 -4.72 -26.99 -34.70 -22.88
Vacuole Periphery -3.77 2.50 0.53 3.85 -1.19 -2.33 10.06 3.60 5.17 -3.59 -4.76 6.35 1.78 5.70 -2.41
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; SPT23 has a paralog, MGA2, that arose from the whole genome duplication
Localization
Cell Percentages ER (19%), cytoplasm (8%), mixed (56%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.00023)
Subcompartmental Group N/A

Spt23

Spt23


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spt23-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available