Standard name
Human Ortholog
Description Subunit of the anaphase-promoting complex/cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 – 0.07 0.05 0 0.1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.1 0 – 0.06 0.07 0.14 0.1 0.07 0.1 0.14 0.09 0.12 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.07 0.08 0.09 0.09 0.24 0.39 – 0.55 0.65 0.6 0.4 0.11 0 0 0.09 0.1 0 0 0 0 0 0 0
Nucleus 0.93 0.87 0.94 0.82 0.58 0.62 0.64 – 0.62 0.59 0.49 0.78 0.79 0.71 0.62 0.65 0.71 0.6 0.85 0.76 0.8 0.75 0.61 0.53
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0.07 0.06 0.2 0.32 0.24 0.18 – 0.2 0.11 0.08 0.05 0.11 0.22 0.19 0.09 0.1 0.09 0.05 0.09 0.07 0.06 0.11 0.1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.25 0.09 0.06 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.12 0.17 0 0.07 0.05 0.11 0.16 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Bud 0 2 0 0 1 0 4 – 2 6 2 1 1 0 0 1 0 0 0 5 0 2 6 11
Bud Neck 0 0 0 0 0 0 2 – 1 1 0 0 0 0 0 0 0 0 0 1 0 0 2 2
Bud Site 0 0 2 0 0 0 5 – 8 6 1 4 0 0 0 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 4 2 1 1 6 4 – 7 9 12 4 20 30 45 5 5 7 0 2 0 3 1 1
Endoplasmic Reticulum 0 1 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 3 0 0 0 0 2 3
Endosome 1 5 1 0 3 1 0 – 0 0 1 0 4 10 12 4 0 2 3 9 0 2 9 18
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 3
Mitochondria 9 30 6 4 5 14 41 – 64 87 51 16 29 4 13 5 4 1 0 2 1 2 6 8
Nucleus 156 398 67 37 31 36 67 – 72 79 42 31 211 214 195 37 29 21 158 380 71 184 249 227
Nuclear Periphery 2 4 0 0 2 0 0 – 1 0 1 0 4 7 4 1 0 1 0 2 0 0 1 9
Nucleolus 9 33 4 9 17 14 19 – 23 15 7 2 30 65 60 5 4 3 9 45 6 15 42 42
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 1 1 3 0 1 0 0 0 0 0 0 0
SpindlePole 0 3 1 0 13 5 6 – 2 2 2 2 2 3 8 0 1 0 3 11 1 3 9 13
Vac/Vac Membrane 1 17 0 0 1 0 1 – 1 2 1 0 4 3 11 4 5 6 6 34 4 25 65 79
Unique Cell Count 167 458 71 45 53 58 104 116 133 85 40 268 302 317 57 41 35 185 498 89 245 408 432
Labelled Cell Count 181 498 84 51 74 76 149 181 207 120 60 306 337 352 65 50 44 185 498 89 245 408 432


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 6.1 5.5 5.0 6.3 4.5 4.6 – 4.1 3.9 3.8 4.3 5.9 6.0 5.9 7.6 7.6 7.7 5.7 5.4 5.9
Std Deviation (1e-4) 1.1 1.1 0.7 0.8 1.0 0.8 1.1 – 1.0 1.0 1.0 1.1 1.4 1.4 1.6 1.5 1.5 1.6 1.5 1.1 1.9
Intensity Change (Log2) – – – -0.14 0.19 -0.29 -0.24 – -0.41 -0.48 -0.53 -0.36 0.1 0.13 0.11 0.48 0.47 0.49 0.05 -0.01 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 2.5 0 0 1.9 2.7 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0.6 0 0 0 0 0
Nucleus -2.1 -4.9 -4.5 -4.6 – -4.9 -5.3 -6.1 -2.7 -3.0 -4.1 -5.3 -4.2 -3.4 -4.4
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.4 3.9 0 0 – 0 0 0 0 1.4 3.1 2.7 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 4.0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.173 4.8839 4.6572 3.9679 4.7187 4.4152 2.5733 3.1018 2.5959 2.4485 2.2933 2.6185 1.1838 2.2568 1.8404 1.4037 1.5851 1.1816
Actin 0.0264 0.0001 0.0057 0 0.0152 0.0001 0.0147 0 0.0087 0 0.0078 0.0008 0.0075 0 0.018 0.008 0 0.0363
Bud 0.0004 0 0.0001 0 0.0005 0 0.0005 0 0.0003 0 0.0011 0.0003 0.0001 0.0001 0 0.0009 0 0.0001
Bud Neck 0.0023 0.0002 0.0009 0.0004 0.0007 0.0013 0.009 0.0001 0.0046 0.0001 0.0026 0.0034 0.0007 0.0001 0.0004 0.0022 0.0005 0.001
Bud Periphery 0.001 0.0003 0.0002 0 0.0009 0.0002 0.0013 0 0.001 0 0.0019 0.0009 0.0004 0.0004 0.0001 0.0014 0.0001 0.0001
Bud Site 0.0058 0.0001 0.0008 0 0.0008 0.0001 0.0042 0 0.0017 0 0.0011 0.001 0.0008 0.0003 0.0002 0.0104 0.0001 0.0001
Cell Periphery 0.0004 0.0001 0.0001 0 0.0002 0 0.0005 0 0.0003 0 0.0002 0.0001 0.0002 0.0001 0 0.0002 0 0
Cytoplasm 0.0081 0.0001 0.0019 0.0001 0.0028 0.0001 0.0109 0.0001 0.0011 0 0.0014 0.004 0.0005 0.0004 0.0003 0.0003 0 0.0002
Cytoplasmic Foci 0.0063 0 0.0048 0 0.0325 0.0001 0.0032 0 0.0014 0 0.0028 0.0004 0.0141 0 0.0003 0.0061 0 0.0003
Eisosomes 0.0004 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0.0002 0 0.0003
Endoplasmic Reticulum 0.0026 0.0001 0.0023 0 0.0109 0.0003 0.0087 0 0.001 0 0.0008 0.0007 0.0014 0 0.0002 0.0002 0 0.001
Endosome 0.0083 0.0001 0.0121 0 0.0549 0.0019 0.0195 0 0.0037 0 0.0173 0.002 0.0029 0.0001 0.0003 0.0066 0 0.0019
Golgi 0.0067 0 0.0115 0 0.0103 0.0005 0.0047 0 0.0017 0 0.0053 0.0021 0.0012 0 0.0003 0.0056 0 0.0029
Lipid Particles 0.0245 0.0001 0.0043 0 0.0198 0.0008 0.0069 0 0.0026 0 0.005 0.0025 0.005 0 0.0001 0.0083 0 0.0007
Mitochondria 0.0252 0.0002 0.0064 0.0001 0.0098 0.0098 0.0129 0.0001 0.0206 0.0001 0.0462 0.0086 0.0024 0.0004 0.0004 0.0034 0.0002 0.001
None 0.0062 0 0.0102 0 0.0016 0.0001 0.0111 0 0.0004 0 0.0007 0.0001 0.0004 0.0002 0.0002 0.0004 0 0.0005
Nuclear Periphery 0.0075 0.0078 0.0141 0.0064 0.0603 0.0079 0.0398 0.0087 0.0109 0.0022 0.0115 0.0022 0.0263 0.0058 0.02 0.0012 0.0004 0.0046
Nucleolus 0.0281 0.0261 0.0402 0.0173 0.0241 0.0446 0.0276 0.0257 0.0369 0.0276 0.0385 0.0513 0.0335 0.0221 0.0336 0.0478 0.067 0.0249
Nucleus 0.8136 0.9556 0.8572 0.9731 0.6989 0.9176 0.7953 0.9498 0.8768 0.9676 0.841 0.9126 0.8668 0.9442 0.8798 0.8195 0.9306 0.9022
Peroxisomes 0.0135 0 0.0026 0 0.025 0 0.0019 0 0.0014 0 0.0023 0.0009 0.0077 0 0.0001 0.0058 0 0.0005
Punctate Nuclear 0.0074 0.0084 0.0233 0.0024 0.0228 0.0128 0.0173 0.0153 0.0217 0.002 0.0069 0.0046 0.0262 0.0249 0.045 0.0699 0.001 0.0212
Vacuole 0.003 0.0004 0.0008 0 0.0049 0.0006 0.0054 0 0.0018 0.0001 0.0035 0.0011 0.0009 0.0005 0.0001 0.0011 0.0001 0.0001
Vacuole Periphery 0.0024 0.0005 0.0004 0 0.0031 0.0009 0.0045 0 0.0011 0.0001 0.002 0.0004 0.0007 0.0002 0.0004 0.0003 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.6214 13.2924 15.0854 15.127 12.4746 11.8016 16.7999 14.8072 15.6674 16.6245
Translational Efficiency 1.1896 1.0267 0.7625 0.8531 0.8742 0.991 0.8325 0.7741 0.7772 0.7718

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1418 1401 2640 1590 2022 1866 1671 880 3440 3267 4311 2470

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 729.99 788.09 1010.65 1053.10 742.23 792.16 921.70 1499.53 737.18 790.41 976.17 1212.15
Standard Deviation 82.17 133.51 140.14 150.10 96.11 114.41 146.84 272.90 90.83 122.98 149.21 294.52
Intensity Change Log 2 — 0.110484 0.469335 0.528694 — 0.093926 0.312431 1.014572 — 0.102160 0.392363 0.793991

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000137 0.000862 0.000884 0.002109 0.000211 0.000508 0.000854 0.000481 0.000180 0.000660 0.000873 0.001529
Bud Neck 0.006180 0.007679 0.026521 0.032735 0.003079 0.007139 0.012780 0.044478 0.004357 0.007371 0.021195 0.036919
Bud Site 0.001122 0.001280 0.001891 0.004527 0.000301 0.001239 0.001270 0.003490 0.000640 0.001257 0.001651 0.004158
Cell Periphery 0.000139 0.000157 0.000067 0.000104 0.000092 0.000096 0.000134 0.000063 0.000111 0.000122 0.000093 0.000089
Cytoplasm 0.008544 0.007284 0.000819 0.004060 0.008150 0.004736 0.005055 0.002819 0.008313 0.005829 0.002461 0.003618
Cytoplasmic Foci 0.000928 0.000808 0.000030 0.000455 0.001104 0.000982 0.000532 0.000029 0.001032 0.000908 0.000225 0.000303
Eisosomes 0.000014 0.000025 0.000032 0.000031 0.000018 0.000023 0.000035 0.000017 0.000016 0.000024 0.000033 0.000026
Endoplasmic Reticulum 0.001868 0.004840 0.002618 0.005219 0.002211 0.004200 0.003916 0.002299 0.002069 0.004475 0.003121 0.004179
Endosome 0.000279 0.000684 0.000212 0.000730 0.000355 0.000726 0.000506 0.000257 0.000324 0.000708 0.000326 0.000561
Golgi 0.000138 0.000700 0.000174 0.000604 0.000174 0.000767 0.000259 0.000151 0.000159 0.000738 0.000207 0.000442
Lipid Particles 0.000362 0.000467 0.000079 0.000250 0.000519 0.000646 0.000402 0.000023 0.000454 0.000569 0.000204 0.000169
Mitochondria 0.002084 0.002687 0.002115 0.006147 0.000751 0.003856 0.001464 0.004564 0.001301 0.003355 0.001863 0.005583
Mitotic Spindle 0.002437 0.005045 0.014152 0.038758 0.000766 0.003165 0.012750 0.010680 0.001455 0.003971 0.013609 0.028754
None 0.005485 0.013453 0.002129 0.004013 0.009925 0.006077 0.011263 0.002135 0.008095 0.009240 0.005669 0.003344
Nuclear Periphery 0.001440 0.001092 0.001348 0.003402 0.000916 0.001059 0.001112 0.005590 0.001132 0.001073 0.001256 0.004182
Nuclear Periphery Foci 0.001806 0.003766 0.000724 0.003067 0.002168 0.000867 0.001237 0.001731 0.002018 0.002110 0.000923 0.002591
Nucleolus 0.070127 0.069884 0.098853 0.057377 0.052330 0.057602 0.097174 0.043965 0.059666 0.062869 0.098202 0.052599
Nucleus 0.895014* 0.876786* 0.846097* 0.831986* 0.916153* 0.903919* 0.847207* 0.875011* 0.907439* 0.892284* 0.846527* 0.847315*
Peroxisomes 0.000342 0.000796 0.000495 0.000538 0.000367 0.000994 0.000862 0.000072 0.000356 0.000909 0.000637 0.000372
Vacuole 0.001401 0.001291 0.000416 0.003177 0.000300 0.000793 0.001015 0.001390 0.000754 0.001006 0.000648 0.002540
Vacuole Periphery 0.000154 0.000414 0.000346 0.000712 0.000111 0.000604 0.000171 0.000755 0.000129 0.000523 0.000278 0.000727

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.62 -21.39 -8.36 -4.87 -4.02 -6.24 -17.75 -10.71 -2.26 7.83 -11.31 -26.73 -9.18 -5.71 -2.98
Bud Neck -1.89 -20.87 -20.26 -19.22 -5.28 -5.96 -15.53 -17.76 -15.95 -12.01 -5.62 -27.28 -27.88 -25.20 -11.85
Bud Site -0.54 -3.41 -5.41 -5.85 -3.32 -3.93 -9.17 -2.96 -1.94 -1.36 -2.87 -7.51 -6.79 -5.44 -3.35
Cell Periphery -0.28 1.25 0.58 1.07 -4.15 -0.17 -1.35 2.08 2.85 4.13 -0.35 0.88 0.97 1.60 0.26
Cytoplasm 0.61 6.87 4.10 2.81 -5.38 2.98 3.78 4.31 0.97 0.61 2.50 8.45 6.08 2.81 -3.21
Cytoplasmic Foci 0.62 3.45 2.81 2.27 -1.79 0.86 2.45 4.74 4.09 2.53 1.08 4.61 5.20 4.32 0.99
Eisosomes -5.82 -14.78 -13.33 -6.23 -0.61 -2.86 -12.66 0.48 3.36 13.38 -5.79 -18.78 -11.00 -4.53 6.19
Endoplasmic Reticulum -9.67 -4.06 -10.31 -1.87 -7.90 -8.68 -8.55 -0.03 8.43 8.30 -13.01 -8.14 -10.31 0.39 -4.21
Endosome -2.07 0.76 -3.83 0.35 -4.92 -2.09 -1.03 1.28 3.03 2.04 -2.91 -0.30 -2.05 1.80 -1.76
Golgi -1.44 -2.65 -3.17 0.53 -2.22 -1.59 -1.38 -0.95 1.41 0.90 -2.14 -2.25 -3.00 1.23 -1.69
Lipid Particles -0.61 2.97 1.59 2.29 -2.88 -0.59 1.30 6.33 3.71 2.20 -0.77 3.43 5.10 3.89 0.67
Mitochondria -0.84 -0.97 -4.96 -4.04 -5.62 -3.63 -9.13 -5.62 -0.73 -4.24 -3.58 -4.28 -8.44 -2.90 -7.09
Mitotic Spindle -1.66 -8.02 -11.12 -10.19 -6.89 -2.28 -7.88 -6.21 -5.32 -0.19 -2.75 -11.77 -12.85 -11.74 -6.31
None -4.10 4.85 1.53 4.98 -5.88 3.23 0.38 5.33 2.68 4.26 -0.64 3.62 5.30 5.48 1.53
Nuclear Periphery 1.34 -0.64 -7.24 -8.28 -7.14 -0.66 -1.09 -5.31 -5.18 -4.90 0.42 -1.83 -8.78 -8.92 -7.93
Nuclear Periphery Foci -0.91 2.55 0.52 1.60 -3.31 1.92 0.27 0.30 -1.72 0.08 0.54 2.24 0.41 -0.17 -2.39
Nucleolus 0.83 -10.89 -2.60 -3.66 8.19 -0.87 -11.88 -4.91 -4.43 4.57 -0.10 -17.44 -6.19 -6.34 9.23
Nucleus 1.73 13.59 13.13 11.74 1.69 1.67 14.29 11.05 9.98 -0.24 2.50 21.17 18.60 16.73 1.36
Peroxisomes -1.88 -3.39 -2.00 1.09 2.30 -2.31 -3.65 4.88 3.58 5.94 -2.95 -5.11 0.02 3.05 6.00
Vacuole 0.08 1.33 -3.08 -3.29 -5.66 -3.87 -3.62 -2.52 -1.04 -0.04 -0.83 -0.30 -4.76 -4.16 -5.19
Vacuole Periphery -2.64 -1.62 -4.18 -0.50 -1.46 -3.08 -3.93 -2.83 0.59 -2.15 -3.91 -2.34 -5.17 0.38 -2.47
Standard name
Human Ortholog
Description Subunit of the anaphase-promoting complex/cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Cdc16

Cdc16


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc16-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available