Standard name
Human Ortholog
Description Uridylate kinase; catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.97 0.96 0.84 0.79 0.79 – 0.75 0.77 0.69 0.73 0.99 0.98 0.98 0.96 0.96 0.96 0.96 0.92 0.93 0.95 0.96 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0 – 0.11 0 0.09 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.06 0 – 0 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.07 0.18 0.15 0.19 – 0.17 0.15 0.18 0.17 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 2 0 1 0 0 0 0 0 0 0 0 2 0 0 1 0
Bud 0 2 0 0 0 2 0 – 4 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 0 0 3 0 6 – 5 6 7 4 1 5 3 11 12 7 0 0 0 0 0 0
Cytoplasm 357 502 91 135 144 92 222 – 194 163 155 133 312 368 486 346 433 443 362 482 101 143 300 328
Endoplasmic Reticulum 0 6 0 0 0 1 4 – 1 5 6 4 3 0 1 22 15 21 0 4 0 2 3 3
Endosome 0 0 0 0 0 0 1 – 0 2 0 0 0 0 0 0 0 1 2 2 0 0 1 1
Golgi 1 0 0 0 0 0 0 – 0 0 0 0 0 3 0 0 0 0 2 0 0 0 0 1
Mitochondria 0 2 0 0 0 10 2 – 29 8 20 16 0 1 1 4 4 2 1 0 0 1 0 0
Nucleus 0 21 3 2 0 7 9 – 9 10 12 10 0 1 3 8 8 15 2 7 2 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 3 0 2 10 31 17 52 – 44 31 41 31 2 1 8 10 20 8 2 19 4 0 1 2
Unique Cell Count 361 516 94 141 171 117 281 260 212 224 182 316 374 495 362 452 461 378 527 109 151 314 343
Labelled Cell Count 362 536 96 147 178 129 296 289 227 243 198 318 380 502 401 492 497 378 527 109 151 314 343


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.1 15.2 14.2 13.5 13.9 7.9 11.9 – 10.5 10.4 10.4 10.1 18.3 18.4 18.3 24.4 25.9 23.0 15.5 16.5 17.0
Std Deviation (1e-4) 3.1 3.1 3.5 2.9 2.2 2.2 2.2 – 2.2 2.5 2.1 2.1 3.4 3.4 3.2 6.9 6.6 6.9 2.9 3.4 3.3
Intensity Change (Log2) – – – -0.08 -0.04 -0.84 -0.26 – -0.44 -0.46 -0.45 -0.5 0.36 0.37 0.36 0.77 0.86 0.69 0.12 0.21 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 -3.1 -3.9 -4.0 – -4.7 -4.3 -5.4 -4.8 1.3 1.0 0.9 -0.5 -0.5 -0.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 3.4 0 3.0 3.0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.8 3.1 3.9 – 3.7 3.3 3.8 3.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.2259 10.8429 10.4533 8.9453 7.9048 9.2754 12.3626 16.3003 14.2093 13.1616 13.3128 13.9795 14.6582 17.644 16.1711 15.7792 16.7003 16.0588
Actin 0.0408 0.0003 0.03 0.0488 0.0939 0.0042 0.0161 0.0001 0.0022 0.0346 0.0035 0.0007 0.009 0.0002 0.0011 0.0001 0.0001 0.0077
Bud 0.0008 0.0001 0.0001 0.0006 0.0007 0.0002 0.0005 0.0002 0.0003 0.0003 0.0001 0.0001 0.0005 0.0001 0.0002 0.0001 0 0.0001
Bud Neck 0.005 0.0001 0.0003 0.0021 0.0016 0.0029 0.0017 0.0002 0.0006 0.0013 0.0007 0.0012 0.0005 0.0001 0.0006 0.0004 0.0003 0.0011
Bud Periphery 0.0011 0 0.0001 0.0004 0.0007 0.0001 0.0006 0 0.0002 0.0004 0.0001 0 0.0012 0 0.0001 0 0 0
Bud Site 0.004 0.0001 0.0004 0.0026 0.0055 0.0003 0.0013 0.0002 0.0008 0.0003 0.0004 0 0.0004 0.0001 0.0002 0.0001 0 0
Cell Periphery 0.0002 0 0 0 0.0001 0 0.0002 0 0 0.0001 0 0 0.0002 0 0 0 0 0
Cytoplasm 0.7506 0.988 0.9542 0.8973 0.7643 0.9267 0.8536 0.9827 0.9478 0.872 0.9033 0.9449 0.9369 0.9913 0.9658 0.9526 0.9873 0.9587
Cytoplasmic Foci 0.0278 0.0006 0.0008 0.0178 0.015 0.01 0.0135 0.0009 0.0035 0.0131 0.0034 0.0025 0.0057 0.0007 0.0051 0.0087 0.0006 0.0014
Eisosomes 0.0003 0 0.0001 0.0001 0.0003 0 0.0002 0 0 0.0006 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0105 0.0005 0.0005 0.0025 0.0023 0.0034 0.0079 0.0008 0.0035 0.0032 0.0015 0.0018 0.0022 0.0003 0.0027 0.0012 0.0003 0.0027
Endosome 0.0273 0.0001 0.0009 0.0027 0.0247 0.0064 0.0246 0.0004 0.0054 0.0155 0.0097 0.0046 0.0039 0.0002 0.0087 0.0071 0.0001 0.0025
Golgi 0.0126 0 0.0009 0.0028 0.0204 0.0027 0.0025 0 0.0006 0.0067 0.0039 0.0011 0.0016 0 0.0011 0.0002 0 0.0004
Lipid Particles 0.0231 0 0.0004 0.0008 0.0193 0.003 0.0063 0 0.0001 0.0081 0.0204 0.0018 0.0067 0 0.0007 0.0006 0 0.0005
Mitochondria 0.0065 0.0001 0.0003 0.0012 0.0059 0.0005 0.003 0.0001 0.0018 0.0017 0.0057 0.0005 0.0024 0.0001 0.0006 0.0001 0 0.0003
None 0.0058 0.0011 0.0009 0.0018 0.0014 0.001 0.0054 0.0009 0.0017 0.0053 0.0015 0.0012 0.0047 0.0008 0.0009 0.0033 0.0008 0.0009
Nuclear Periphery 0.0277 0.0007 0.0009 0.0024 0.0045 0.0047 0.0148 0.0011 0.0039 0.005 0.0034 0.0036 0.0029 0.0004 0.0014 0.0021 0.0005 0.0063
Nucleolus 0.0005 0 0 0 0.0001 0.0001 0.0005 0 0.0002 0.0002 0.0007 0 0.0001 0 0 0.0002 0 0
Nucleus 0.0292 0.0079 0.0086 0.0064 0.0124 0.0276 0.0304 0.0115 0.0248 0.0216 0.0332 0.0338 0.0119 0.0054 0.0089 0.0183 0.0097 0.0158
Peroxisomes 0.0096 0 0.0003 0.0067 0.0226 0.0015 0.0032 0 0.0002 0.0019 0.0042 0.0002 0.0061 0 0.0004 0.0001 0 0.0006
Punctate Nuclear 0.0125 0.0002 0.0003 0.0023 0.0025 0.0037 0.0057 0.0004 0.0011 0.0066 0.0019 0.0015 0.0025 0.0001 0.0006 0.0034 0.0002 0.0006
Vacuole 0.002 0.0001 0.0001 0.0005 0.001 0.0007 0.0062 0.0003 0.0009 0.0009 0.0012 0.0004 0.0004 0.0001 0.0008 0.0011 0.0001 0.0004
Vacuole Periphery 0.0021 0 0.0001 0.0001 0.0007 0.0003 0.0017 0 0.0003 0.0005 0.0009 0.0001 0.0001 0 0.0002 0.0002 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 165.0621 147.5555 138.0558 149.5027 137.2457 156.0049 150.8148 125.8692 147.4025 131.7283
Translational Efficiency 1.3368 1.6685 1.5151 1.3693 1.6672 1.6644 1.504 2.26 1.5558 1.496

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1349 1260 1969 1684 1786 1332 2422 1964 3135 2592 4391 3648

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1204.54 1346.33 1709.89 1748.86 1187.61 1359.38 1732.45 1940.70 1194.90 1353.04 1722.33 1852.14
Standard Deviation 218.11 274.61 265.17 298.35 180.96 211.77 265.70 342.45 197.98 244.43 265.70 336.71
Intensity Change Log 2 — 0.160550 0.505421 0.537933 — 0.194888 0.544753 0.708516 — 0.177699 0.525082 0.625141

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000178 0.000340 0.000076 0.000159 0.000108 0.000413 0.000116 0.000834 0.000138 0.000378 0.000098 0.000523
Bud Neck 0.000538 0.000608 0.000661 0.000851 0.000409 0.000675 0.000504 0.002907 0.000465 0.000642 0.000575 0.001958
Bud Site 0.003378 0.003092 0.001885 0.004266 0.002105 0.005787 0.002097 0.015747 0.002653 0.004477 0.002002 0.010447
Cell Periphery 0.000099 0.000098 0.000043 0.000151 0.000046 0.000048 0.000067 0.000102 0.000069 0.000072 0.000056 0.000124
Cytoplasm 0.878523* 0.820763* 0.739666* 0.778994* 0.871970* 0.865665* 0.804711* 0.773965* 0.874789* 0.843838* 0.775544* 0.776287*
Cytoplasmic Foci 0.032033 0.014661 0.001443 0.003616 0.019272 0.018710 0.003941 0.011795 0.024763 0.016742 0.002821 0.008019
Eisosomes 0.000004 0.000004 0.000001 0.000002 0.000003 0.000004 0.000001 0.000005 0.000004 0.000004 0.000001 0.000003
Endoplasmic Reticulum 0.000656 0.000574 0.000061 0.000072 0.000419 0.000374 0.000053 0.000327 0.000521 0.000472 0.000056 0.000210
Endosome 0.000708 0.000782 0.000051 0.000514 0.000570 0.001228 0.000248 0.004601 0.000629 0.001011 0.000160 0.002714
Golgi 0.000647 0.000676 0.000070 0.000939 0.000471 0.001429 0.000724 0.010775 0.000547 0.001063 0.000431 0.006234
Lipid Particles 0.000304 0.000337 0.000120 0.000483 0.000218 0.000347 0.000239 0.000873 0.000255 0.000342 0.000186 0.000693
Mitochondria 0.000489 0.000286 0.000195 0.002541 0.000237 0.000260 0.000159 0.000704 0.000345 0.000273 0.000176 0.001552
Mitotic Spindle 0.001776 0.002615 0.002902 0.004690 0.001775 0.004655 0.002632 0.040464 0.001776 0.003663 0.002753 0.023950
None 0.003519 0.011089 0.010872 0.008457 0.008064 0.009835 0.008832 0.008703 0.006109 0.010445 0.009747 0.008589
Nuclear Periphery 0.000105 0.000090 0.000118 0.000082 0.000063 0.000054 0.000067 0.000255 0.000081 0.000071 0.000090 0.000176
Nuclear Periphery Foci 0.003095 0.002933 0.000272 0.000923 0.002238 0.001990 0.000829 0.001597 0.002606 0.002448 0.000579 0.001286
Nucleolus 0.000159 0.000102 0.000081 0.000115 0.000108 0.000372 0.000083 0.000270 0.000130 0.000241 0.000082 0.000199
Nucleus 0.056796 0.129586 0.236293* 0.174118* 0.080533 0.074056 0.164955* 0.102154 0.070319 0.101050 0.196944* 0.135375*
Peroxisomes 0.000111 0.000305 0.000017 0.000033 0.000084 0.000637 0.000123 0.001253 0.000096 0.000476 0.000076 0.000690
Vacuole 0.015758 0.010922 0.005119 0.016376 0.010997 0.012511 0.008942 0.020528 0.013046 0.011739 0.007227 0.018612
Vacuole Periphery 0.001123 0.000136 0.000054 0.002618 0.000309 0.000949 0.000675 0.002140 0.000659 0.000553 0.000396 0.002361

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.14 1.68 -2.34 1.66 -3.29 -2.35 -2.33 -1.98 0.86 -1.39 -3.19 -0.53 -2.42 1.53 -2.21
Bud Neck -1.88 -2.26 -3.94 -2.86 -1.86 -2.09 -4.79 -8.92 -6.81 -6.37 -2.92 -4.91 -9.56 -7.34 -6.20
Bud Site -0.17 2.53 -0.89 -0.58 -2.88 -3.94 -1.34 -6.26 -1.40 -6.27 -3.52 0.56 -5.85 -1.60 -6.69
Cell Periphery -0.43 1.69 -2.48 -3.35 -6.87 -0.84 -8.90 -9.44 -8.40 -1.28 -0.93 -1.21 -5.42 -6.49 -6.45
Cytoplasm 10.63 30.14 23.75 11.05 -5.71 2.56 22.05 24.91 19.40 7.15 9.53 36.68 34.48 21.51 1.64
Cytoplasmic Foci 7.68 17.35 14.55 6.86 -10.71 0.24 11.79 8.44 6.37 -4.91 5.54 20.79 16.50 9.20 -9.11
Eisosomes -0.55 8.42 3.33 2.09 -8.46 -1.29 3.02 -0.97 0.65 -7.03 -1.38 6.42 0.55 1.97 -10.24
Endoplasmic Reticulum 0.22 4.16 4.45 5.05 2.69 0.44 5.27 4.36 2.74 -1.69 0.33 6.42 6.20 5.51 -0.49
Endosome -0.52 9.77 5.23 3.24 -1.88 -1.91 3.54 -1.49 0.29 -3.06 -1.94 7.04 -0.09 1.46 -3.38
Golgi -0.09 4.61 1.64 1.36 -2.00 -2.24 0.33 -2.07 -0.93 -2.15 -2.03 2.18 -1.73 -0.63 -2.40
Lipid Particles -2.56 -0.55 -6.78 -5.09 -6.30 -2.00 -6.43 -11.94 -8.88 -7.85 -3.19 -5.56 -13.12 -9.91 -9.89
Mitochondria 0.86 1.29 -0.50 -0.85 -0.92 -0.02 0.80 0.12 0.25 -1.44 0.63 1.46 -0.48 -0.79 -1.09
Mitotic Spindle -1.23 -3.29 -1.27 -0.01 1.48 -2.11 -0.62 -6.25 -3.95 -6.12 -2.41 -2.47 -6.20 -3.66 -4.78
None -8.61 -10.70 -7.10 5.01 5.79 -1.00 0.81 1.99 2.40 1.86 -5.18 -5.43 -1.18 4.92 5.64
Nuclear Periphery -0.29 -4.43 -1.83 -4.35 6.30 0.50 -8.45 -15.56 -15.99 -12.43 -0.38 -6.64 -10.76 -15.82 -7.34
Nuclear Periphery Foci -0.62 11.00 6.79 6.59 -6.93 0.48 5.65 4.43 3.30 -1.68 -0.39 11.52 7.89 7.15 -4.96
Nucleolus 1.20 1.24 0.96 -0.30 -0.37 -1.41 0.62 -4.65 0.99 -7.53 -1.19 1.38 -1.30 0.94 -4.77
Nucleus -17.52 -44.34 -29.42 -8.59 14.96 1.38 -27.52 -11.85 -12.24 14.60 -12.32 -49.61 -28.63 -13.40 20.06
Peroxisomes -0.81 4.05 3.51 1.22 -2.87 -1.98 0.22 -1.62 1.19 -1.65 -2.10 1.59 -1.05 1.61 -1.71
Vacuole 0.98 0.80 -14.05 -14.07 -14.51 -3.69 -9.10 -23.94 -21.04 -19.76 -2.18 -6.28 -27.53 -25.11 -24.16
Vacuole Periphery 2.40 2.61 0.57 -1.41 -1.60 -1.09 -0.12 -0.81 0.81 -0.46 0.46 2.07 0.20 -0.26 -1.58
Standard name
Human Ortholog
Description Uridylate kinase; catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ura6

Ura6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ura6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available