Standard name
Human Ortholog
Description Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex; poly (A) mRNA binding subunit which recruits mRNA to the complex; the Pan2p-Pan3p complex controls poly(A) tail length and regulates the stoichiometry and activity of postreplication repair complexes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.97 0.96 0.97 0.98 0.93 0.85 0.81 0.8 0.63 0.62 0.56 0.6 1.0 0.99 1.0 – – – 0.94 0.92 0.86 0.95 0.94 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0 0 0 0.05 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.13 0.15 0.4 0.32 0.54 0.33 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0 0.08 0.13 0.06 0.07 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 1 0 1 0 0 9 4 3 1 3 0 0 0 – – – 1 1 1 0 1 0
Bud 1 1 2 0 0 2 1 3 2 1 2 3 0 0 0 – – – 0 1 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 1 3 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – – – – – – – – –
Cell Periphery 4 6 9 3 3 3 16 11 10 10 6 9 2 7 6 – – – 0 0 0 0 0 0
Cytoplasm 210 296 196 162 183 172 247 210 165 170 121 139 167 329 307 – – – 209 290 180 174 220 265
Endoplasmic Reticulum 4 7 4 3 1 5 18 7 11 4 10 7 0 1 0 – – – 4 2 4 0 1 2
Endosome 0 2 0 0 0 2 1 3 1 5 0 2 0 2 0 – – – 1 4 3 0 0 3
Golgi 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 – – – 1 2 1 1 0 1
Mitochondria 0 1 0 1 3 22 39 39 103 89 117 77 0 0 0 – – – 0 1 3 0 2 3
Nucleus 0 1 0 1 0 3 0 2 3 3 2 3 1 2 1 – – – 0 0 0 0 0 3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 1 1 1 0 0 0
Nucleolus 0 1 0 0 1 0 0 0 0 0 0 0 0 2 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 6 1 3 9 6 12 10 21 35 12 17 0 0 0 – – – 1 5 5 2 3 2
Unique Cell Count 216 309 202 166 196 202 305 263 260 274 215 231 167 334 308 224 317 210 184 235 286
Labelled Cell Count 220 322 213 173 201 216 334 295 320 321 271 261 170 344 314 224 317 210 184 235 286


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 9.4 8.6 8.6 9.6 7.8 7.8 8.2 6.6 7.1 6.5 6.8 8.7 9.0 8.8 – – – 8.3 8.5 8.8
Std Deviation (1e-4) 0.9 1.3 1.3 1.8 2.3 1.6 1.4 1.9 1.2 1.5 1.6 1.6 1.0 1.4 1.2 – – – 1.4 1.5 1.4
Intensity Change (Log2) – – – -0.0 0.16 -0.14 -0.14 -0.06 -0.37 -0.26 -0.4 -0.33 0.02 0.08 0.04 – – – -0.04 -0.01 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery -1.4 -1.7 -1.8 0.4 -0.1 -0.3 -0.4 -0.9 -0.3 0 -1.6 -1.6 – – –
Cytoplasm 0.3 -1.7 -4.2 -5.3 -5.5 -8.7 -8.9 -9.7 -9.1 2.2 1.2 2.5 – – –
Endoplasmic Reticulum 0 0 0 2.1 0 1.4 0 1.5 0.7 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 4.8 5.3 5.7 10.1 9.0 12.4 9.0 0 0 0 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 2.4 0 3.8 5.0 3.0 3.6 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9258 4.0585 3.5837 3.2651 3.3431 3.8878 2.992 4.5565 4.0259 3.6283 3.0916 3.8867 4.3546 5.8782 5.3343 5.5834 5.0928 5.0725
Actin 0.0241 0.0041 0.0085 0.0045 0.0045 0.0052 0.0276 0.0008 0.0031 0.0135 0.0002 0.0053 0.0207 0.0004 0.0044 0.0001 0.0021 0.0007
Bud 0.0043 0.0032 0.0042 0.0016 0.0126 0.0038 0.001 0.0003 0.0036 0.0007 0.0003 0.0035 0.0003 0.0008 0.0015 0.0001 0.0006 0.0022
Bud Neck 0.0018 0.0002 0.0006 0.0008 0.0008 0.001 0.0031 0.0003 0.0005 0.0051 0.0005 0.007 0.0022 0.0005 0.0008 0.0002 0.0006 0.0016
Bud Periphery 0.0019 0.0014 0.0033 0.0009 0.0126 0.0032 0.0021 0.0001 0.002 0.0006 0.0002 0.0028 0.0003 0.0003 0.0011 0 0.0003 0.0006
Bud Site 0.0052 0.0018 0.0049 0.0109 0.0082 0.0006 0.0032 0.0092 0.0036 0.002 0.0005 0.0017 0.0007 0.0129 0.007 0.0001 0.0003 0.0005
Cell Periphery 0.0004 0.0002 0.0004 0.0002 0.0004 0.0001 0.0007 0.0001 0.0002 0.0002 0.0001 0.0002 0.0002 0.0002 0.0002 0 0 0.0001
Cytoplasm 0.7535 0.9527 0.8254 0.6757 0.7083 0.8903 0.7641 0.9414 0.8427 0.8059 0.8634 0.8915 0.8375 0.935 0.9199 0.9215 0.8751 0.9244
Cytoplasmic Foci 0.0284 0.008 0.0173 0.0583 0.0259 0.0164 0.0366 0.009 0.0347 0.0317 0.0345 0.0215 0.0235 0.0141 0.0125 0.0117 0.0221 0.0174
Eisosomes 0.0002 0 0.0001 0.0001 0 0 0.0004 0 0 0.0001 0 0 0.0002 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0262 0.0037 0.0133 0.0098 0.0036 0.0115 0.0087 0.0046 0.005 0.0025 0.002 0.005 0.0172 0.0041 0.0068 0.0066 0.0101 0.0068
Endosome 0.0426 0.0076 0.0453 0.0853 0.1089 0.0351 0.0456 0.011 0.0383 0.0668 0.0495 0.0234 0.0334 0.0114 0.0191 0.0451 0.0521 0.0309
Golgi 0.0112 0.0009 0.0136 0.0457 0.0421 0.0054 0.0151 0.0011 0.0092 0.0281 0.0075 0.0055 0.0066 0.001 0.0019 0.0006 0.0039 0.0024
Lipid Particles 0.0091 0.0001 0.0026 0.0323 0.0213 0.0032 0.0094 0.0001 0.0017 0.0041 0.0201 0.0039 0.0049 0.0002 0.0012 0.0001 0.0063 0.0002
Mitochondria 0.0028 0.0006 0.019 0.0455 0.0161 0.0047 0.0153 0.0006 0.0039 0.0044 0.0082 0.0038 0.0025 0.0007 0.0011 0.0004 0.0021 0.0012
None 0.0436 0.011 0.0271 0.0037 0.0024 0.0037 0.0447 0.0177 0.0365 0.0054 0.0021 0.0134 0.0228 0.0131 0.0063 0.0027 0.003 0.0032
Nuclear Periphery 0.0038 0.0006 0.0019 0.001 0.0023 0.0029 0.0032 0.0006 0.0011 0.0011 0.0005 0.0009 0.0064 0.0005 0.0027 0.0016 0.0087 0.0011
Nucleolus 0.0014 0 0.0001 0.0002 0.0003 0.0008 0.0003 0 0.0001 0.0005 0.0004 0.0002 0.0003 0.0002 0.0001 0 0.0006 0.0004
Nucleus 0.0049 0.0009 0.0014 0.001 0.0022 0.0029 0.0012 0.001 0.0015 0.0012 0.001 0.0011 0.0028 0.0011 0.0069 0.0013 0.0028 0.0014
Peroxisomes 0.011 0.0001 0.0011 0.0131 0.0022 0.0015 0.0106 0.0001 0.0064 0.0049 0.0029 0.0028 0.0088 0.0002 0.0012 0 0.0008 0.0002
Punctate Nuclear 0.005 0.0002 0.0005 0.0005 0.0005 0.0008 0.0015 0.0002 0.0008 0.0006 0.0005 0.004 0.003 0.0005 0.0012 0.0001 0.0035 0.0004
Vacuole 0.0164 0.0023 0.0071 0.0065 0.0183 0.0051 0.0044 0.0016 0.0042 0.0167 0.0042 0.0017 0.0047 0.0025 0.0033 0.0064 0.0033 0.0034
Vacuole Periphery 0.002 0.0003 0.0022 0.0024 0.0063 0.0019 0.0012 0.0003 0.0009 0.004 0.0014 0.0006 0.0011 0.0004 0.0007 0.0013 0.0015 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.5221 21.7583 12.9165 23.4708 14.0256 15.1364 23.8331 24.0874 17.0516 24.1551
Translational Efficiency 1.5045 1.101 1.5776 0.6729 1.2989 1.6508 1.1639 1.0075 1.3375 1.1114

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2050 1032 1410 135 1482 1901 103 478 3532 2933 1513 613

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 780.03 870.03 1115.64 1265.41 922.46 923.33 1175.61 1528.55 839.79 904.58 1119.72 1470.60
Standard Deviation 88.76 138.54 130.99 138.91 168.19 128.57 129.85 239.06 146.23 134.60 131.78 246.39
Intensity Change Log 2 — 0.157536 0.516270 0.698003 — 0.001360 0.349851 0.728605 — 0.075019 0.428489 0.714665

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000144 0.000210 0.000331 0.001027 0.000296 0.000360 0.000226 0.001533 0.000208 0.000307 0.000324 0.001421
Bud Neck 0.008834 0.002137 0.000915 0.002424 0.005994 0.004352 0.002185 0.005421 0.007642 0.003572 0.001001 0.004761
Bud Site 0.010269 0.010817 0.011736 0.028533 0.014070 0.018552 0.006290 0.023273 0.011864 0.015830 0.011365 0.024431
Cell Periphery 0.000130 0.000085 0.000067 0.000197 0.000154 0.000068 0.000222 0.000050 0.000140 0.000074 0.000078 0.000083
Cytoplasm 0.629946* 0.699009* 0.849497* 0.635939* 0.718087* 0.680436* 0.430241* 0.875679* 0.666929* 0.686971* 0.820955* 0.822882*
Cytoplasmic Foci 0.201059* 0.141541 0.038707 0.081234 0.122188 0.171596 0.108962 0.008676 0.167966 0.161021 0.043490 0.024655
Eisosomes 0.000093 0.000022 0.000008 0.000023 0.000044 0.000027 0.000095 0.000016 0.000073 0.000025 0.000014 0.000018
Endoplasmic Reticulum 0.001131 0.002764 0.000610 0.000758 0.001171 0.001121 0.003340 0.000021 0.001148 0.001699 0.000796 0.000183
Endosome 0.011134 0.016432 0.002200 0.004352 0.007646 0.013713 0.028144 0.000745 0.009670 0.014670 0.003966 0.001539
Golgi 0.005860 0.003254 0.000449 0.001097 0.009336 0.008568 0.000585 0.000725 0.007319 0.006699 0.000458 0.000807
Lipid Particles 0.003805 0.002775 0.000643 0.001616 0.002243 0.001226 0.013245 0.000122 0.003150 0.001771 0.001501 0.000451
Mitochondria 0.002628 0.001033 0.000089 0.000091 0.003025 0.002144 0.000393 0.001490 0.002795 0.001753 0.000110 0.001182
Mitotic Spindle 0.001363 0.002192 0.003017 0.011307 0.001295 0.006113 0.000932 0.002609 0.001335 0.004734 0.002875 0.004524
None 0.005528 0.003077 0.001915 0.000825 0.007674 0.001042 0.000399 0.002012 0.006429 0.001758 0.001812 0.001751
Nuclear Periphery 0.000121 0.000327 0.000138 0.000493 0.000058 0.000053 0.001453 0.000019 0.000094 0.000150 0.000228 0.000124
Nuclear Periphery Foci 0.000481 0.002375 0.002620 0.010728 0.000455 0.000215 0.012515 0.000048 0.000470 0.000975 0.003294 0.002400
Nucleolus 0.000597 0.001662 0.000092 0.000085 0.000642 0.000227 0.000416 0.000240 0.000616 0.000732 0.000114 0.000206
Nucleus 0.041731 0.026945 0.044375 0.019401 0.029503 0.010408 0.057462 0.028698 0.036600 0.016227 0.045266 0.026651
Peroxisomes 0.001542 0.001195 0.000539 0.000645 0.001284 0.001643 0.001795 0.001750 0.001434 0.001485 0.000625 0.001507
Vacuole 0.071740 0.078014 0.041846 0.191038 0.070789 0.074688 0.330179* 0.046533 0.071341 0.075858 0.061474 0.078357
Vacuole Periphery 0.001862 0.004134 0.000205 0.008188 0.004045 0.003446 0.000922 0.000339 0.002778 0.003688 0.000253 0.002067

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.65 -3.00 -2.33 -2.07 -1.52 -0.31 0.44 -1.00 -0.93 -1.07 -0.88 -1.59 -1.27 -1.13 -1.08
Bud Neck 8.98 10.78 8.21 -1.08 -4.75 3.00 5.60 1.59 -0.63 -3.39 8.03 13.99 5.03 -1.35 -6.41
Bud Site -0.45 -0.59 -3.50 -3.23 -2.93 -2.80 5.23 -1.62 -0.28 -3.71 -3.69 0.59 -3.03 -1.48 -3.04
Cell Periphery 7.93 10.72 -0.47 -2.37 -2.88 11.90 -1.35 14.82 5.01 5.60 15.27 11.18 10.67 1.27 2.21
Cytoplasm -6.97 -26.92 6.19 8.59 13.75 4.79 10.66 -18.17 -22.05 -15.94 -3.13 -18.70 -10.87 -8.75 0.98
Cytoplasmic Foci 9.63 37.66 19.86 10.73 -3.04 -8.79 2.72 28.33 38.15 8.33 1.72 37.24 43.62 38.71 9.52
Eisosomes 17.04 21.12 14.88 -1.72 -7.69 5.56 -3.43 3.86 1.69 5.21 16.72 21.00 8.82 1.44 -0.32
Endoplasmic Reticulum -3.86 2.72 3.22 5.53 1.00 0.17 -2.30 6.33 3.46 4.16 -1.97 2.42 8.35 6.28 7.98
Endosome -3.65 15.83 10.88 8.92 -3.43 -6.88 -2.45 13.48 17.12 3.59 -6.50 10.03 20.23 19.04 4.48
Golgi 5.09 20.25 16.71 5.78 -0.06 1.03 16.19 15.43 12.02 -0.48 1.32 25.17 21.04 13.00 -0.82
Lipid Particles 2.91 12.79 3.66 1.22 -3.70 6.45 -1.34 15.15 13.85 1.72 7.60 4.16 14.95 8.28 2.04
Mitochondria 2.64 4.62 4.69 3.76 1.85 1.64 8.57 4.00 1.44 -2.00 2.23 8.10 4.05 1.72 -2.74
Mitotic Spindle -0.84 -1.87 -2.37 -2.21 -2.06 -3.34 0.76 -0.43 1.77 -0.71 -3.39 -2.07 -1.81 -0.03 -0.95
None 3.16 7.24 9.67 4.02 8.90 7.19 7.91 6.19 -3.94 -7.02 8.80 9.58 9.56 0.60 0.72
Nuclear Periphery -2.87 -3.27 -3.22 -1.31 -2.64 0.40 -2.24 3.62 3.58 2.30 -2.02 -4.41 -1.33 0.44 2.81
Nuclear Periphery Foci -9.02 -11.60 -6.21 -4.65 -4.14 4.29 -4.05 7.38 8.25 4.21 -6.19 -11.33 -4.54 -2.84 4.06
Nucleolus -1.33 11.72 11.85 1.99 1.09 12.62* 4.37 10.80 -1.42 0.86 -0.39 17.56 12.65 1.83 -6.71
Nucleus 6.87 -4.35 6.69 1.54 9.08 16.12 -2.60 -0.35 -9.17 2.42 18.31 -7.59 4.17 -6.48 9.22
Peroxisomes 1.90 4.93 8.72 3.84 -0.24 -3.06 -0.12 -0.38 0.40 -0.11 -0.52 4.53 0.49 0.63 -1.76
Vacuole -1.73 2.75 -12.76 -12.34 -13.12 -1.52 -11.29 3.62 4.90 11.80 -2.22 -2.74 -6.88 -6.09 -5.01
Vacuole Periphery -2.89 16.71 -0.12 0.95 -0.96 1.75 10.08 14.83 11.74 1.86 -2.60 21.06 4.61 5.50 -1.05
Standard name
Human Ortholog
Description Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex; poly (A) mRNA binding subunit which recruits mRNA to the complex; the Pan2p-Pan3p complex controls poly(A) tail length and regulates the stoichiometry and activity of postreplication repair complexes
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Pan3

Pan3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pan3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available