Standard name
Human Ortholog
Description TFIIE large subunit; involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0.05 0.08 0.11 0.08 0.05 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0.05 0 0 0.13 0.08 0.15 0.2 0.19 0.19 0.25 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.95 0.92 0.93 0.91 0.91 0.89 0.85 0.78 0.8 0.77 0.73 0.76 0.94 0.85 0.77 0.83 0.9 0.83 0.92 0.91 0.92 0.91 0.77 0.69
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.18 0.15 0.16 0.1 0.08 0.14 0.17 0.25 0.22 0.33 0.45 0.26 0.08 0.08 0.15 0.05 0 0 0 0 0 0 0.1 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 0 0 0 1 2 0 0 2 11
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 1 0 2 1 3 6 7 2 10 1 1 6 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 6 3 8 16 6 14 30 12 16 5 13 30 84 114 32 16 24 1 1 0 0 2 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 4 0 0 0 0 0 1
Endosome 0 0 1 1 0 0 0 1 0 0 1 0 1 7 16 7 4 0 0 0 1 1 8 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 2 2 1
Mitochondria 6 11 8 6 8 42 41 102 76 123 55 116 0 2 0 18 3 4 1 1 0 1 4 5
Nucleus 232 165 143 151 288 287 440 551 310 513 207 356 612 850 831 320 281 204 222 170 146 509 662 730
Nuclear Periphery 0 0 0 0 0 1 2 1 1 10 1 6 0 0 0 4 0 0 0 0 0 0 0 2
Nucleolus 44 27 24 17 25 45 86 177 84 216 127 120 54 84 157 18 14 11 7 1 4 23 87 122
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 3 11 14 4 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 1 0 0 0 0 0 3 27 32 3 0 1 1 0 0 0 3 3
Vac/Vac Membrane 0 3 0 1 2 3 2 7 4 0 1 3 1 9 22 8 6 9 4 5 5 15 77 161
Unique Cell Count 243 180 153 166 316 321 518 703 388 664 283 470 653 1003 1078 384 313 246 243 187 159 559 858 1064
Labelled Cell Count 287 212 179 186 339 386 588 873 494 886 401 625 706 1078 1193 418 325 258 243 187 159 559 858 1064


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.3 9.3 8.5 9.0 8.9 7.4 7.4 6.9 7.0 6.5 6.3 6.2 13.0 12.0 11.8 10.5 13.5 14.5 11.4 10.8 11.8
Std Deviation (1e-4) 2.2 2.5 1.7 2.0 1.8 1.6 1.6 1.5 1.7 1.5 1.6 1.6 3.5 3.7 3.9 3.3 3.8 3.8 2.3 2.2 2.3
Intensity Change (Log2) – – – 0.08 0.06 -0.21 -0.19 -0.3 -0.28 -0.39 -0.42 -0.45 0.61 0.5 0.47 0.31 0.67 0.77 0.43 0.35 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 1.6 0 0 1.3 0 0 0 0 1.5 2.8 3.4 2.7 1.6 3.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.7 -1.5 2.6 1.1 3.1 4.2 4.0 4.0 5.2 0 0 0 -0.3 0 0
Nucleus -0.8 -0.9 -1.4 -2.7 -4.3 -3.9 -4.5 -5.1 -4.8 0.1 -2.9 -4.7 -3.1 -1.3 -3.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.5 -2.6 -0.5 0.3 2.5 1.6 4.1 6.1 2.5 -2.8 -2.9 -0.4 -4.3 -4.2 -3.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.7793 12.4744 12.1278 11.6448 11.2601 11.8943 6.184 6.0409 5.9775 6.2954 3.1755 6.1234 7.213 7.1756 6.7525 7.6457 7.4812 7.926
Actin 0.0005 0 0.0014 0.0001 0.0019 0.0003 0.005 0 0.0194 0 0.0908 0.015 0.007 0 0.0238 0 0 0
Bud 0.0002 0 0.0001 0 0.0001 0 0.0008 0 0.0001 0 0.0011 0 0.0005 0 0.0002 0 0 0
Bud Neck 0.0002 0 0.0005 0 0.0005 0.0004 0.0003 0.0001 0.0001 0.0002 0.0007 0.0003 0.0009 0.0001 0.0004 0 0.0002 0.0004
Bud Periphery 0.0004 0 0.0001 0 0.0001 0 0.0009 0 0.0001 0 0.001 0 0.0012 0 0.0005 0 0.0001 0.0001
Bud Site 0.0004 0 0.0014 0 0.0006 0.0001 0.0017 0.0002 0.0011 0 0.0028 0.0001 0.0008 0.0001 0.0016 0 0 0
Cell Periphery 0.0002 0 0.0001 0 0 0 0.0005 0 0 0 0.0001 0 0.0006 0.0001 0.0002 0 0 0
Cytoplasm 0.003 0 0.0016 0.0001 0.0006 0.0003 0.0079 0.0247 0.0008 0 0.0052 0 0.0028 0.0072 0.0004 0.0001 0 0
Cytoplasmic Foci 0.0043 0 0.0008 0.0028 0.0079 0.0001 0.0361 0.0002 0.0089 0 0.0296 0.0004 0.0072 0.0001 0.0108 0 0 0
Eisosomes 0 0 0 0 0 0 0 0 0.0001 0 0.0003 0.0001 0.0001 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0004 0 0.0019 0.0001 0.0002 0.0002 0.0018 0.0002 0.0002 0 0.0035 0.0001 0.005 0.0002 0.0007 0 0 0
Endosome 0.0012 0 0.0034 0.0005 0.0066 0.0001 0.0074 0.0019 0.0078 0 0.045 0.0003 0.015 0.0005 0.0036 0 0 0
Golgi 0.0003 0 0.0007 0.0003 0.0049 0 0.0029 0 0.003 0 0.0243 0.0006 0.0017 0 0.0013 0 0 0
Lipid Particles 0.0038 0 0.0006 0.0041 0.0064 0.0001 0.022 0 0.0027 0 0.0113 0.0007 0.0026 0 0.0041 0 0 0
Mitochondria 0.0019 0.0001 0.0007 0 0.0023 0.0005 0.0032 0.0001 0.0006 0.0002 0.0093 0.0001 0.0017 0.0002 0.0099 0 0.0003 0.0002
None 0.0011 0 0.0002 0.0001 0.0002 0.0005 0.0032 0.0003 0.0209 0 0.0033 0.0001 0.0018 0.0001 0.0006 0 0 0
Nuclear Periphery 0.0032 0.0015 0.0031 0.0035 0.0029 0.0008 0.0236 0.0036 0.0013 0.0016 0.0054 0.0003 0.0142 0.0051 0.0065 0.0031 0.0006 0.0001
Nucleolus 0.0456 0.0162 0.0136 0.0077 0.1068 0.0779 0.0188 0.0111 0.0052 0.0626 0.0609 0.0485 0.0536 0.0199 0.0186 0.0052 0.0779 0.0502
Nucleus 0.9247 0.9809 0.9659 0.9528 0.8469 0.9175 0.8018 0.9418 0.9239 0.9348 0.6874 0.9323 0.8424 0.9576 0.9046 0.9908 0.9201 0.9486
Peroxisomes 0.0002 0 0.0001 0.0001 0.005 0 0.0176 0 0.0028 0 0.0117 0.0005 0.0031 0 0.0052 0 0 0
Punctate Nuclear 0.0055 0.0011 0.0027 0.0278 0.0054 0.0009 0.0215 0.0007 0.0006 0.0003 0.0045 0.0004 0.0333 0.0014 0.0031 0.0001 0.0004 0.0001
Vacuole 0.0017 0 0.0008 0 0.0004 0.0001 0.0132 0.0138 0.0002 0.0001 0.0008 0 0.0031 0.0063 0.0016 0.0003 0.0001 0.0001
Vacuole Periphery 0.0013 0 0.0003 0 0.0003 0.0001 0.0097 0.0012 0.0001 0.0001 0.0009 0 0.0015 0.0011 0.0022 0.0002 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.3635 40.163 36.8745 38.1747 36.5305 31.965 33.7259 41.3445 47.6491 35.0598
Translational Efficiency 0.8855 0.8267 0.7565 1.0403 0.7643 0.873 0.8133 0.6865 0.5929 0.6961

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description TFIIE large subunit; involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Tfa1

Tfa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tfa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available