Standard name
Human Ortholog
Description UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; involved in PKA-mediated oxidative stress resistance and long-term survival in stationary phase; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0.05 0 0.08 0.05 0.07 0.05 0.08 0.1 0.05 0 0.05 0 0.07 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.99 0.97 0.94 0.88 0.86 0.85 0.85 0.79 0.85 1.0 0.99 0.99 0.95 0.96 0.97 0.99 0.97 0.9 0.94 0.95 0.93
Endoplasmic Reticulum 0 0 0 0 0 0.06 0.07 0.09 0.08 0.06 0.13 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 2 5 5 3 6 6 0 0 0 0 0 0 0 0 1 0 1 1
Bud 0 2 0 0 0 1 0 0 3 0 2 0 1 1 0 1 0 0 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 14 7 3 9 5 15 12 11 6 13 17 18 19 19 13 14 6 0 0 0 0 0 0
Cytoplasm 144 432 141 147 175 175 168 202 142 111 129 148 370 447 410 295 205 172 142 423 132 199 376 260
Endoplasmic Reticulum 1 4 1 1 4 12 13 21 13 8 22 13 2 3 6 12 7 5 0 2 2 2 4 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0
Golgi 0 1 0 0 0 0 1 0 1 1 2 0 0 0 0 0 1 0 0 0 0 0 1 1
Mitochondria 1 0 0 1 1 0 4 3 8 9 11 9 1 0 0 1 0 0 0 1 1 2 1 1
Nucleus 0 0 1 1 0 1 1 1 0 0 1 0 0 1 0 1 0 0 0 0 2 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 1 1 1 0 2 6 0 2 1 1 0 2 0 16 5 3 0 1 1 0 1 3
Unique Cell Count 146 436 143 148 181 186 191 236 167 131 163 175 371 452 413 309 214 177 144 438 147 212 397 281
Labelled Cell Count 147 453 151 154 190 195 206 250 183 140 187 194 392 473 435 339 232 186 144 438 147 212 397 281


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.0 26.2 20.0 19.9 24.6 23.6 25.1 22.8 23.8 21.3 23.7 23.5 28.8 31.7 34.0 25.7 35.9 31.5 19.3 20.0 20.4
Std Deviation (1e-4) 4.3 5.2 4.5 4.2 6.0 6.6 6.8 6.7 6.7 7.0 7.2 7.6 7.4 9.2 10.5 8.7 11.2 11.1 5.1 4.4 4.6
Intensity Change (Log2) – – – -0.0 0.3 0.24 0.33 0.19 0.26 0.09 0.25 0.23 0.53 0.67 0.77 0.37 0.85 0.66 -0.05 0.0 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 -1.1 1.1 0.1 0.6 -0.1 1.1 1.6 0 -0.4 -0.1 -0.3 0.6 -0.7
Cytoplasm 0.6 -1.1 -2.1 -3.7 -4.2 -4.2 -4.2 -5.3 -4.3 1.5 0.3 0.7 -1.7 -1.5 -0.9
Endoplasmic Reticulum 0 0 2.7 2.8 3.3 3.0 0 4.2 2.9 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 3.2 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.3 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 24.1257 35.2858 32.1439 27.2064 29.223 34.7093 30.7131 40.1118 34.3381 34.1525 32.788 39.1637 32.9117 42.0043 39.187 38.1864 35.5131 38.4875
Actin 0.0348 0.0004 0.0037 0.103 0.064 0.0002 0.0059 0.0089 0.0148 0.0374 0.022 0.0003 0.005 0.0004 0.0031 0.0002 0.0019 0.0007
Bud 0.0008 0.0053 0.0013 0.0016 0.0007 0.0001 0.0009 0.0002 0.0007 0.001 0.0003 0.0001 0.0008 0.0024 0.0006 0.0003 0.0003 0.0002
Bud Neck 0.0011 0.0002 0.0009 0.0018 0.0007 0.0005 0.0005 0.0001 0.0004 0.0008 0.0004 0.0006 0.0004 0.0001 0.0003 0.0003 0.0003 0.0008
Bud Periphery 0.001 0.0015 0.0016 0.0018 0.0005 0 0.0008 0.0001 0.0004 0.0008 0.0003 0 0.0005 0.001 0.0014 0.0002 0.0002 0.0004
Bud Site 0.0019 0.0048 0.0054 0.0088 0.0022 0 0.001 0.0004 0.0009 0.0043 0.0005 0 0.0006 0.0007 0.0008 0.0002 0.0003 0.0001
Cell Periphery 0.0012 0.0001 0.0003 0.0003 0.0001 0 0.0001 0.0001 0.0002 0.0002 0.0001 0 0.0008 0.0002 0.0005 0.0002 0.0001 0.0002
Cytoplasm 0.7512 0.9677 0.9401 0.8124 0.8522 0.9574 0.8715 0.9824 0.8634 0.8929 0.851 0.9921 0.9044 0.9814 0.9621 0.9274 0.9125 0.9746
Cytoplasmic Foci 0.0145 0.0025 0.0162 0.0035 0.0177 0.0006 0.0346 0.0012 0.0099 0.0221 0.0094 0.0018 0.0129 0.0016 0.0063 0.0044 0.0169 0.0021
Eisosomes 0.0002 0 0.0001 0.0008 0.0006 0 0 0 0.0004 0.0002 0.0002 0 0.0003 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0186 0.0006 0.0026 0.0011 0.001 0.0362 0.0052 0.0006 0.0031 0.0006 0.0014 0.0011 0.0101 0.0003 0.0016 0.0028 0.0035 0.0027
Endosome 0.0371 0.0014 0.0035 0.0043 0.007 0.0007 0.0242 0.0005 0.0183 0.0164 0.0053 0.0017 0.0072 0.0003 0.0033 0.0095 0.0057 0.0035
Golgi 0.0105 0.0003 0.0038 0.0091 0.0037 0 0.0109 0.0009 0.0387 0.0078 0.0258 0.0001 0.0026 0.0001 0.0006 0.0004 0.0013 0.0012
Lipid Particles 0.0124 0.0001 0.0017 0.0029 0.0089 0 0.0026 0.0003 0.004 0.0015 0.0021 0 0.0055 0.0001 0.0028 0.0017 0.0066 0.0017
Mitochondria 0.0076 0.0002 0.0027 0.0392 0.022 0.0002 0.0084 0.0019 0.0197 0.0065 0.0736 0.0001 0.0048 0.0086 0.0039 0.0015 0.0033 0.0004
None 0.02 0.009 0.0047 0.0003 0.0012 0.0001 0.0036 0.0002 0.0015 0.0005 0.0002 0.0002 0.0069 0.0004 0.0032 0.0018 0.0065 0.0003
Nuclear Periphery 0.0364 0.0007 0.001 0.0016 0.0029 0.0016 0.0068 0.0002 0.0152 0.0004 0.0004 0.0004 0.0048 0.0003 0.0012 0.0206 0.0077 0.0013
Nucleolus 0.001 0.0002 0.0002 0.0001 0.001 0 0.0005 0 0.0001 0.0001 0 0 0.0015 0.0001 0.0005 0.001 0.0007 0.0001
Nucleus 0.0141 0.0025 0.0017 0.0016 0.0024 0.0015 0.0084 0.0005 0.0029 0.0009 0.0007 0.0008 0.0104 0.0008 0.0016 0.0106 0.0057 0.003
Peroxisomes 0.0062 0 0.0027 0.0038 0.0031 0 0.0069 0.0012 0.0027 0.0019 0.0054 0 0.0033 0.0002 0.0006 0.0001 0.0021 0.0005
Punctate Nuclear 0.0075 0.0009 0.0002 0.0002 0.0024 0 0.0017 0 0.0006 0.0002 0.0001 0 0.0033 0.0001 0.0006 0.001 0.0193 0.0004
Vacuole 0.0161 0.0013 0.0051 0.0012 0.004 0.0005 0.0033 0.0002 0.0012 0.003 0.0007 0.0003 0.011 0.0008 0.0038 0.0106 0.0037 0.0047
Vacuole Periphery 0.0059 0.0002 0.0006 0.0005 0.0014 0.0002 0.0021 0 0.001 0.0005 0.0002 0.0001 0.0029 0.0002 0.0012 0.005 0.0013 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 205.9688 172.9446 141.5072 183.657 166.4279 184.5341 202.2905 199.021 189.9722 157.6848
Translational Efficiency 1.4756 1.8211 1.6526 1.4663 1.7175 2.045 1.6212 1.4797 1.5938 1.4678

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; involved in PKA-mediated oxidative stress resistance and long-term survival in stationary phase; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ugp1

Ugp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ugp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available