Standard name | |
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Human Ortholog | |
Description | Protein involved in Fe-S cluster transfer to mitochondrial clients; protects [4Fe-4S] clusters from damage due to oxidative stress; acts along with Bol3 at a late step in the transfer of [4Fe-4S] clusters from the ISA complex to client proteins; Fe-S loaded homodimer at steady state; similar to NifU, a bacterial protein required for Fe/S cluster maturation; ortholog of the human NFU1, mutations of which are associated with Multiple Mitochondria Dysfunctions Syndrome (MMDS1) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 3 | 4 | 1 | 3 | 3 | 0 | 1 | 1 | 1 | 6 | 7 | 5 | – | – | 1 | 1 | 2 | 1 | 1 | 3 | 7 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 6 | 1 | 3 | 2 | 5 | 5 | 0 | 1 | 4 | – | – | 1 | 0 | 0 | 1 | 3 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 7 | 2 | 3 | 2 | 2 | 1 | 2 | 0 | 0 | 2 | 8 | 9 | – | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 6 | 1 | 5 | 14 | 7 | 5 | 6 | 1 | 5 | 3 | 1 | 9 | – | – | 0 | 0 | 0 | 0 | 1 | 2 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 4 | 0 | 3 | 3 | 3 | 2 | 1 | 5 | – | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Endosome | 0 | 0 | 0 | 1 | 0 | 2 | 6 | 2 | 1 | 0 | 0 | 4 | 1 | 3 | 3 | – | – | 0 | 0 | 1 | 2 | 0 | 2 | 5 |
Golgi | 0 | 0 | 21 | 21 | 62 | 16 | 15 | 35 | 1 | 13 | 2 | 7 | 26 | 70 | 115 | – | – | 11 | 0 | 10 | 4 | 6 | 34 | 32 |
Mitochondria | 17 | 58 | 84 | 96 | 111 | 126 | 164 | 209 | 137 | 202 | 131 | 221 | 77 | 210 | 238 | – | – | 1 | 49 | 52 | 100 | 46 | 147 | 183 |
Nucleus | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 2 | 2 | 1 | 1 | 3 | 0 | 0 | 0 | – | – | 1 | 0 | 0 | 0 | 0 | 2 | 0 |
Nuclear Periphery | 1 | 6 | 4 | 3 | 5 | 12 | 17 | 15 | 8 | 21 | 12 | 16 | 2 | 12 | 14 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 5 | 5 | 4 | 3 | 8 | 16 | 31 | 24 | 16 | 27 | 24 | 32 | 0 | 7 | 3 | – | – | 3 | 2 | 1 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 2 | 0 | 2 | 1 | 0 | 0 | 4 | 2 | 0 | 0 | 1 | 4 | 4 | 2 | – | – | 0 | 3 | 1 | 2 | 0 | 1 | 7 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 3 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 9 | 9 | 28 | 20 | 33 | 32 | 8 | 13 | 2 | 15 | 4 | 9 | 23 | – | – | 7 | 0 | 3 | 1 | 1 | 10 | 5 |
Unique Cell Count | 17 | 60 | 97 | 111 | 146 | 160 | 225 | 274 | 155 | 241 | 146 | 260 | 88 | 241 | 291 | 25 | 59 | 74 | 115 | 65 | 214 | 253 | ||
Labelled Cell Count | 23 | 73 | 126 | 153 | 224 | 202 | 293 | 341 | 185 | 292 | 184 | 315 | 127 | 336 | 430 | 27 | 59 | 74 | 115 | 65 | 214 | 253 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.0 | 14.0 | 14.7 | 15.2 | 16.3 | 13.6 | 13.0 | 13.3 | 10.7 | 11.7 | 9.8 | 10.7 | 16.6 | 15.7 | 14.9 | – | – | 21.0 | 16.8 | 17.4 | 17.5 |
Std Deviation (1e-4) | 1.9 | 2.9 | 3.3 | 3.3 | 3.1 | 2.8 | 2.7 | 3.0 | 2.7 | 2.9 | 2.9 | 2.6 | 4.1 | 3.0 | 2.8 | – | – | 5.8 | 4.7 | 4.0 | 4.6 |
Intensity Change (Log2) | – | – | – | 0.05 | 0.15 | -0.12 | -0.17 | -0.15 | -0.46 | -0.33 | -0.59 | -0.45 | 0.17 | 0.1 | 0.02 | – | – | 0.52 | 0.19 | 0.25 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Golgi | -0.5 | 3.4 | -2.6 | -3.9 | -2.1 | -5.7 | -4.5 | -5.3 | -5.9 | 1.2 | 1.4 | 3.2 | – | – | 2.3 |
Mitochondria | 0 | -2.0 | -1.6 | -2.7 | -2.1 | 0.4 | -0.6 | 0.7 | -0.4 | 0.2 | 0.1 | -1.1 | – | – | -8.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Nuclear Periphery | 0 | 0 | 1.1 | 1.1 | 0 | 0 | 1.5 | 1.3 | 0.7 | 0 | 0 | 0 | – | – | 0 |
Nucleolus | 0 | 0 | 1.7 | 2.6 | 1.5 | 1.8 | 2.0 | 2.9 | 2.3 | 0 | 0 | 0 | – | – | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Vacuole | -0.3 | 2.1 | 0.8 | 1.3 | 0.6 | -1.3 | -1.3 | 0 | -1.2 | -1.3 | -2.1 | -0.4 | – | – | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 13.2584 | 12.8632 | 12.3534 | 10.6094 | 11.1981 | 12.6154 | 13.2736 | 13.6048 | 12.0173 | 11.2901 | 10.678 | 11.8783 | 11.3402 | 13.2942 | 11.7193 | 11.5601 | 11.0259 | 13.0496 |
Actin | 0.0044 | 0.0058 | 0.0005 | 0.0001 | 0.0018 | 0.0019 | 0.0405 | 0.0004 | 0.0173 | 0.002 | 0.0096 | 0.0004 | 0.0202 | 0.0123 | 0.0113 | 0.0002 | 0.0004 | 0.0004 |
Bud | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0009 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0003 | 0.0004 | 0 | 0 |
Bud Periphery | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0006 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0002 | 0.0015 | 0.0001 | 0.0002 | 0.0002 |
Bud Site | 0.0001 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0.0011 | 0.0001 | 0.0052 | 0.0001 | 0.0005 | 0 | 0.0012 | 0.001 | 0.0022 | 0.0002 | 0.0005 | 0.0001 |
Cell Periphery | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0.0014 | 0.0003 | 0.0007 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0007 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0004 | 0.0014 | 0.0001 | 0.0049 | 0.0001 | 0.0033 | 0.0012 | 0.0262 | 0.0025 | 0.0103 | 0.0031 | 0.004 | 0.0002 |
Eisosomes | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.007 | 0.0002 | 0.0007 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 |
Endosome | 0.0004 | 0.0007 | 0.0012 | 0.0002 | 0.0022 | 0.0007 | 0.0056 | 0.0011 | 0.0113 | 0.0001 | 0.0069 | 0.0067 | 0.0091 | 0.0029 | 0.0109 | 0.0008 | 0.0025 | 0.0022 |
Golgi | 0.0051 | 0.0009 | 0.0072 | 0.0005 | 0.0031 | 0.0042 | 0.0036 | 0.0005 | 0.0022 | 0.0003 | 0.0052 | 0.0043 | 0.0041 | 0.0084 | 0.0093 | 0.0007 | 0.0017 | 0.0024 |
Lipid Particles | 0.0029 | 0.0018 | 0.0035 | 0.001 | 0.0206 | 0.0044 | 0.0088 | 0.0011 | 0.0077 | 0.0002 | 0.0181 | 0.0009 | 0.0461 | 0.0364 | 0.0164 | 0.0147 | 0.0247 | 0.015 |
Mitochondria | 0.9781 | 0.9568 | 0.9799 | 0.9961 | 0.9167 | 0.9804 | 0.9097 | 0.9684 | 0.9335 | 0.9938 | 0.9156 | 0.9808 | 0.8605 | 0.9046 | 0.9007 | 0.9455 | 0.9438 | 0.9386 |
None | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0017 | 0 | 0.0022 | 0 | 0.0002 | 0 | 0.0003 | 0 |
Nuclear Periphery | 0 | 0.0002 | 0.0004 | 0 | 0.0002 | 0.0009 | 0.0001 | 0 | 0.0001 | 0 | 0.0138 | 0 | 0.0029 | 0.0001 | 0.0012 | 0 | 0.0002 | 0.0005 |
Nucleolus | 0 | 0.0002 | 0 | 0 | 0.0003 | 0.0004 | 0.0004 | 0.0001 | 0.0048 | 0 | 0.0003 | 0.0002 | 0.0019 | 0.0039 | 0.0002 | 0.0035 | 0.0034 | 0.0005 |
Nucleus | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0 | 0.0013 | 0.0001 | 0.0006 | 0.0002 | 0.0007 | 0.0002 | 0.0006 | 0.0002 |
Peroxisomes | 0.0043 | 0.0025 | 0.0009 | 0.0001 | 0.0054 | 0.001 | 0.0078 | 0.0011 | 0.004 | 0.0027 | 0.0025 | 0.0015 | 0.0092 | 0.0086 | 0.0097 | 0.0242 | 0.0052 | 0.0007 |
Punctate Nuclear | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0025 | 0 | 0.0088 | 0 | 0.0007 | 0 | 0.0003 | 0 |
Vacuole | 0.0002 | 0.0061 | 0.0003 | 0.0002 | 0.01 | 0.0004 | 0.0134 | 0.0083 | 0.003 | 0 | 0.0015 | 0.0009 | 0.001 | 0.0029 | 0.0032 | 0.0023 | 0.0028 | 0.0029 |
Vacuole Periphery | 0.0034 | 0.0234 | 0.0056 | 0.0013 | 0.0381 | 0.0044 | 0.0058 | 0.0181 | 0.0024 | 0.0001 | 0.0078 | 0.0023 | 0.0036 | 0.0152 | 0.019 | 0.0037 | 0.0088 | 0.0356 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 50.8053 | 40.9391 | 51.2638 | 46.9298 | 45.4604 | 55.7833 | 54.3289 | 50.2007 | 50.5858 | 48.0865 |
Translational Efficiency | 1.9426 | 2.2148 | 1.6715 | 2.3732 | 1.8577 | 1.4267 | 1.8441 | 2.2796 | 1.8842 | 1.8147 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1742 | 1082 | 1536 | 1510 | 1828 | 2103 | 1268 | 1054 | 3570 | 3185 | 2804 | 2564 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1228.45 | 1566.24 | 1467.38 | 1924.72 | 1241.37 | 1414.59 | 1628.95 | 1725.74 | 1235.07 | 1466.11 | 1540.44 | 1842.92 |
Standard Deviation | 271.46 | 315.43 | 317.51 | 441.11 | 234.75 | 267.48 | 402.31 | 363.85 | 253.41 | 293.60 | 367.27 | 422.61 |
Intensity Change Log 2 | — | 0.350466 | 0.256403 | 0.647809 | — | 0.188451 | 0.392009 | 0.475282 | — | 0.271308 | 0.326153 | 0.563672 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001802 | 0.005358 | 0.001845 | 0.006472 | 0.001026 | 0.001249 | 0.004704 | 0.007984 | 0.001405 | 0.002645 | 0.003138 | 0.007093 |
Bud Neck | 0.000914 | 0.002473 | 0.003952 | 0.001705 | 0.001379 | 0.000725 | 0.006893 | 0.002054 | 0.001152 | 0.001319 | 0.005282 | 0.001848 |
Bud Site | 0.001121 | 0.001211 | 0.006255 | 0.003370 | 0.001449 | 0.001131 | 0.012888 | 0.002299 | 0.001289 | 0.001158 | 0.009255 | 0.002930 |
Cell Periphery | 0.000443 | 0.000083 | 0.000556 | 0.000713 | 0.000055 | 0.000140 | 0.000694 | 0.000211 | 0.000244 | 0.000121 | 0.000618 | 0.000506 |
Cytoplasm | 0.000206 | 0.000277 | 0.000821 | 0.000251 | 0.000165 | 0.000149 | 0.001432 | 0.000268 | 0.000185 | 0.000192 | 0.001098 | 0.000258 |
Cytoplasmic Foci | 0.005710 | 0.026237 | 0.015926 | 0.008831 | 0.014215 | 0.007066 | 0.026966 | 0.014130 | 0.010065 | 0.013579 | 0.020918 | 0.011009 |
Eisosomes | 0.000830 | 0.000894 | 0.000201 | 0.001016 | 0.000530 | 0.000399 | 0.000800 | 0.002161 | 0.000676 | 0.000567 | 0.000472 | 0.001487 |
Endoplasmic Reticulum | 0.000109 | 0.000218 | 0.000173 | 0.000981 | 0.000160 | 0.000098 | 0.000256 | 0.000375 | 0.000135 | 0.000139 | 0.000210 | 0.000732 |
Endosome | 0.001806 | 0.002830 | 0.009730 | 0.002182 | 0.002033 | 0.002507 | 0.007307 | 0.003888 | 0.001922 | 0.002617 | 0.008634 | 0.002883 |
Golgi | 0.006921 | 0.026281 | 0.020779 | 0.031084 | 0.012273 | 0.012488 | 0.016713 | 0.032506 | 0.009661 | 0.017174 | 0.018940 | 0.031668 |
Lipid Particles | 0.002076 | 0.006928 | 0.005275 | 0.004218 | 0.006205 | 0.004000 | 0.004063 | 0.006003 | 0.004190 | 0.004995 | 0.004727 | 0.004952 |
Mitochondria | 0.963311* | 0.896015* | 0.874762* | 0.894723* | 0.938950* | 0.950794* | 0.849076* | 0.902819* | 0.950837* | 0.932184* | 0.863147* | 0.898051* |
Mitotic Spindle | 0.000241 | 0.000714 | 0.006622 | 0.000756 | 0.000250 | 0.000336 | 0.003576 | 0.000742 | 0.000246 | 0.000464 | 0.005245 | 0.000751 |
None | 0.001893 | 0.001878 | 0.001623 | 0.003457 | 0.002126 | 0.001409 | 0.001915 | 0.003114 | 0.002012 | 0.001568 | 0.001755 | 0.003316 |
Nuclear Periphery | 0.000102 | 0.000415 | 0.001413 | 0.000661 | 0.000323 | 0.000168 | 0.000908 | 0.000535 | 0.000215 | 0.000252 | 0.001184 | 0.000609 |
Nuclear Periphery Foci | 0.000114 | 0.000493 | 0.000951 | 0.000255 | 0.000365 | 0.000152 | 0.000908 | 0.000269 | 0.000243 | 0.000267 | 0.000931 | 0.000261 |
Nucleolus | 0.000263 | 0.001253 | 0.001993 | 0.000815 | 0.000495 | 0.000586 | 0.002318 | 0.000353 | 0.000381 | 0.000813 | 0.002140 | 0.000625 |
Nucleus | 0.000051 | 0.000279 | 0.000285 | 0.000116 | 0.000108 | 0.000149 | 0.000470 | 0.000072 | 0.000080 | 0.000194 | 0.000369 | 0.000098 |
Peroxisomes | 0.006975 | 0.016401 | 0.008306 | 0.006016 | 0.010626 | 0.005465 | 0.017988 | 0.008010 | 0.008845 | 0.009180 | 0.012684 | 0.006836 |
Vacuole | 0.001995 | 0.005515 | 0.018963 | 0.007276 | 0.002932 | 0.006446 | 0.021246 | 0.003384 | 0.002475 | 0.006129 | 0.019995 | 0.005676 |
Vacuole Periphery | 0.003119 | 0.004248 | 0.019569 | 0.025102 | 0.004334 | 0.004543 | 0.018880 | 0.008824 | 0.003741 | 0.004443 | 0.019258 | 0.018410 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.86 | -0.10 | -4.31 | -0.74 | -4.56 | -0.99 | -4.96 | -5.73 | -5.52 | -2.32 | -2.61 | -3.99 | -7.14 | -5.17 | -4.69 |
Bud Neck | -2.35 | -6.36 | -2.32 | 1.11 | 4.51 | 1.59 | -6.21 | -1.12 | -2.65 | 5.16 | -0.53 | -8.52 | -2.11 | -1.57 | 6.87 |
Bud Site | -0.21 | -5.45 | -2.45 | -2.30 | 2.26 | 0.50 | -6.16 | -1.07 | -1.68 | 5.62 | 0.33 | -8.02 | -2.55 | -2.82 | 5.68 |
Cell Periphery | 3.06 | -0.38 | -0.97 | -2.48 | -0.42 | -2.14 | -3.68 | -4.65 | -1.42 | 2.75 | 1.97 | -2.09 | -1.64 | -2.54 | 0.50 |
Cytoplasm | -1.04 | -4.31 | -0.66 | 0.46 | 4.21 | 0.49 | -4.68 | -2.07 | -2.34 | 4.27 | -0.21 | -6.30 | -1.73 | -1.96 | 5.82 |
Cytoplasmic Foci | -8.91 | -6.92 | -2.49 | 7.14 | 4.25 | 5.57 | -5.02 | 0.04 | -4.72 | 4.84 | -3.21 | -7.61 | -0.91 | 2.10 | 6.56 |
Eisosomes | -0.31 | 3.31 | -0.89 | -1.06 | -9.68 | 2.45 | -1.43 | -5.92 | -6.46 | -4.16 | 1.08 | 1.62 | -5.22 | -7.26 | -6.86 |
Endoplasmic Reticulum | -1.78 | -2.32 | -2.26 | -1.95 | -2.09 | 1.34 | -1.58 | -2.04 | -2.84 | -1.13 | -0.11 | -2.32 | -2.57 | -2.55 | -2.24 |
Endosome | -2.34 | -8.38 | -0.80 | 1.27 | 7.71 | -1.00 | -5.60 | -2.07 | -1.55 | 2.84 | -2.10 | -9.99 | -2.07 | -0.57 | 7.63 |
Golgi | -7.45 | -6.22 | -8.24 | -1.30 | -3.00 | -0.13 | -2.07 | -5.97 | -5.90 | -4.34 | -5.42 | -5.91 | -9.88 | -6.07 | -5.09 |
Lipid Particles | -4.51 | -4.19 | -2.72 | 2.21 | 1.10 | 2.06 | 2.11 | 0.16 | -1.67 | -1.68 | -1.09 | -0.87 | -1.06 | 0.05 | -0.28 |
Mitochondria | 9.14 | 13.17 | 10.80 | 0.17 | -2.46 | -2.42 | 10.80 | 4.93 | 6.86 | -5.60 | 4.75 | 16.54 | 10.89 | 6.56 | -5.60 |
Mitotic Spindle | -2.88 | -6.36 | -2.88 | -0.25 | 5.80 | -0.71 | -5.47 | -2.67 | -1.93 | 4.50 | -2.15 | -8.13 | -3.98 | -2.00 | 7.21 |
None | 0.08 | 1.71 | -7.43 | -7.51 | -9.84 | 5.45 | 1.63 | -4.03 | -7.29 | -5.15 | 4.10 | 2.51 | -8.30 | -11.72 | -10.76 |
Nuclear Periphery | -2.73 | -4.44 | -7.25 | -1.80 | 2.48 | 1.47 | -3.29 | -1.15 | -2.15 | 1.68 | -0.52 | -5.33 | -4.27 | -3.72 | 3.00 |
Nuclear Periphery Foci | -2.66 | -3.96 | -3.57 | 1.64 | 3.28 | 2.25 | -2.04 | 0.89 | -1.90 | 2.48 | -0.36 | -4.10 | -0.33 | 0.11 | 4.09 |
Nucleolus | -2.62 | -3.90 | -2.35 | 1.01 | 2.42 | -0.50 | -3.53 | 1.12 | 1.37 | 3.84 | -2.46 | -5.21 | -1.65 | 0.88 | 4.25 |
Nucleus | -1.54 | -4.52 | -2.26 | 1.09 | 2.99 | -0.78 | -3.37 | 1.04 | 1.62 | 3.77 | -1.88 | -5.09 | -0.74 | 1.58 | 4.75 |
Peroxisomes | -4.10 | -0.92 | 0.61 | 4.55 | 1.58 | 3.26 | -3.38 | 1.27 | -1.40 | 4.24 | -0.27 | -2.99 | 1.59 | 1.83 | 4.43 |
Vacuole | -3.71 | -11.64 | -6.21 | -1.50 | 7.23 | -3.59 | -10.04 | -0.72 | 2.97 | 9.66 | -5.12 | -15.24 | -5.64 | 0.52 | 11.68 |
Vacuole Periphery | -1.62 | -11.12 | -10.31 | -9.83 | -2.20 | -0.28 | -7.50 | -4.15 | -4.10 | 4.86 | -1.34 | -12.99 | -10.94 | -10.46 | 0.52 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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