Standard name
Human Ortholog
Description Protein involved in Fe-S cluster transfer to mitochondrial clients; protects [4Fe-4S] clusters from damage due to oxidative stress; acts along with Bol3 at a late step in the transfer of [4Fe-4S] clusters from the ISA complex to client proteins; Fe-S loaded homodimer at steady state; similar to NifU, a bacterial protein required for Fe/S cluster maturation; ortholog of the human NFU1, mutations of which are associated with Multiple Mitochondria Dysfunctions Syndrome (MMDS1)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.22 0.19 0.42 0.1 0.07 0.13 0 0.05 0 0 0.3 0.29 0.4 0.44 0 0.14 0 0.1 0.16 0.13
Mitochondria 1.0 0.97 0.87 0.86 0.76 0.79 0.73 0.76 0.88 0.84 0.9 0.85 0.88 0.87 0.82 0 0.83 0.71 0.87 0.71 0.69 0.72
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0.1 0 0 0 0.07 0.08 0.05 0.05 0.09 0.08 0.06 0 0.05 0.05 0 0 0 0 0 0 0
Nucleolus 0.29 0.08 0 0 0.05 0.1 0.14 0.09 0.1 0.11 0.16 0.12 0 0 0 0.12 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.09 0.08 0.19 0.12 0.15 0.12 0.05 0.05 0 0.06 0.05 0 0.08 0.28 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 3 4 1 3 3 0 1 1 1 6 7 5 1 1 2 1 1 3 7
Bud 0 0 0 0 1 0 6 1 3 2 5 5 0 1 4 1 0 0 1 3 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 1 1 1 1 0 0 0 0
Cell Periphery 0 0 2 7 2 3 2 2 1 2 0 0 2 8 9 2 0 0 0 0 0 0
Cytoplasm 0 0 0 6 1 5 14 7 5 6 1 5 3 1 9 0 0 0 0 1 2 0
Endoplasmic Reticulum 0 0 0 0 1 1 0 4 0 3 3 3 2 1 5 0 0 0 0 0 2 0
Endosome 0 0 0 1 0 2 6 2 1 0 0 4 1 3 3 0 0 1 2 0 2 5
Golgi 0 0 21 21 62 16 15 35 1 13 2 7 26 70 115 11 0 10 4 6 34 32
Mitochondria 17 58 84 96 111 126 164 209 137 202 131 221 77 210 238 1 49 52 100 46 147 183
Nucleus 0 0 2 1 0 0 2 2 2 1 1 3 0 0 0 1 0 0 0 0 2 0
Nuclear Periphery 1 6 4 3 5 12 17 15 8 21 12 16 2 12 14 0 0 0 0 0 0 2
Nucleolus 5 5 4 3 8 16 31 24 16 27 24 32 0 7 3 3 2 1 0 0 0 1
Peroxisomes 0 2 0 2 1 0 0 4 2 0 0 1 4 4 2 0 3 1 2 0 1 7
SpindlePole 0 0 0 1 0 0 0 1 0 0 1 1 0 3 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 9 9 28 20 33 32 8 13 2 15 4 9 23 7 0 3 1 1 10 5
Unique Cell Count 17 60 97 111 146 160 225 274 155 241 146 260 88 241 291 25 59 74 115 65 214 253
Labelled Cell Count 23 73 126 153 224 202 293 341 185 292 184 315 127 336 430 27 59 74 115 65 214 253


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.0 14.0 14.7 15.2 16.3 13.6 13.0 13.3 10.7 11.7 9.8 10.7 16.6 15.7 14.9 21.0 16.8 17.4 17.5
Std Deviation (1e-4) 1.9 2.9 3.3 3.3 3.1 2.8 2.7 3.0 2.7 2.9 2.9 2.6 4.1 3.0 2.8 5.8 4.7 4.0 4.6
Intensity Change (Log2) 0.05 0.15 -0.12 -0.17 -0.15 -0.46 -0.33 -0.59 -0.45 0.17 0.1 0.02 0.52 0.19 0.25 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -0.5 3.4 -2.6 -3.9 -2.1 -5.7 -4.5 -5.3 -5.9 1.2 1.4 3.2 2.3
Mitochondria 0 -2.0 -1.6 -2.7 -2.1 0.4 -0.6 0.7 -0.4 0.2 0.1 -1.1 -8.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1.1 1.1 0 0 1.5 1.3 0.7 0 0 0 0
Nucleolus 0 0 1.7 2.6 1.5 1.8 2.0 2.9 2.3 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.3 2.1 0.8 1.3 0.6 -1.3 -1.3 0 -1.2 -1.3 -2.1 -0.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.2584 12.8632 12.3534 10.6094 11.1981 12.6154 13.2736 13.6048 12.0173 11.2901 10.678 11.8783 11.3402 13.2942 11.7193 11.5601 11.0259 13.0496
Actin 0.0044 0.0058 0.0005 0.0001 0.0018 0.0019 0.0405 0.0004 0.0173 0.002 0.0096 0.0004 0.0202 0.0123 0.0113 0.0002 0.0004 0.0004
Bud 0 0.0001 0 0.0001 0.0001 0 0.0003 0 0.0003 0.0001 0.0002 0 0.0002 0.0001 0.0009 0.0001 0.0001 0.0001
Bud Neck 0 0 0 0 0 0 0.0002 0 0.0009 0 0.0001 0 0.0003 0.0001 0.0003 0.0004 0 0
Bud Periphery 0.0001 0.0002 0.0001 0.0003 0.0004 0.0001 0.0004 0.0001 0.0006 0.0002 0.0003 0 0.0003 0.0002 0.0015 0.0001 0.0002 0.0002
Bud Site 0.0001 0.0005 0 0 0.0001 0.0001 0.0011 0.0001 0.0052 0.0001 0.0005 0 0.0012 0.001 0.0022 0.0002 0.0005 0.0001
Cell Periphery 0 0.0003 0.0001 0.0001 0.0002 0.0001 0.0002 0.0002 0.0004 0 0.0002 0.0001 0.0001 0.0002 0.0002 0.0002 0.0002 0.0002
Cytoplasm 0 0 0 0 0 0 0.0002 0 0.0003 0 0.0014 0.0003 0.0007 0.0001 0.0004 0 0.0001 0
Cytoplasmic Foci 0.0007 0.0001 0.0002 0 0.0004 0.0004 0.0014 0.0001 0.0049 0.0001 0.0033 0.0012 0.0262 0.0025 0.0103 0.0031 0.004 0.0002
Eisosomes 0.0002 0.0002 0.0001 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002 0.0003 0 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0 0 0 0 0.0001 0.0001 0.0001 0 0.0001 0 0.007 0.0002 0.0007 0.0001 0.0006 0 0.0001 0
Endosome 0.0004 0.0007 0.0012 0.0002 0.0022 0.0007 0.0056 0.0011 0.0113 0.0001 0.0069 0.0067 0.0091 0.0029 0.0109 0.0008 0.0025 0.0022
Golgi 0.0051 0.0009 0.0072 0.0005 0.0031 0.0042 0.0036 0.0005 0.0022 0.0003 0.0052 0.0043 0.0041 0.0084 0.0093 0.0007 0.0017 0.0024
Lipid Particles 0.0029 0.0018 0.0035 0.001 0.0206 0.0044 0.0088 0.0011 0.0077 0.0002 0.0181 0.0009 0.0461 0.0364 0.0164 0.0147 0.0247 0.015
Mitochondria 0.9781 0.9568 0.9799 0.9961 0.9167 0.9804 0.9097 0.9684 0.9335 0.9938 0.9156 0.9808 0.8605 0.9046 0.9007 0.9455 0.9438 0.9386
None 0 0 0 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0017 0 0.0022 0 0.0002 0 0.0003 0
Nuclear Periphery 0 0.0002 0.0004 0 0.0002 0.0009 0.0001 0 0.0001 0 0.0138 0 0.0029 0.0001 0.0012 0 0.0002 0.0005
Nucleolus 0 0.0002 0 0 0.0003 0.0004 0.0004 0.0001 0.0048 0 0.0003 0.0002 0.0019 0.0039 0.0002 0.0035 0.0034 0.0005
Nucleus 0 0.0001 0 0 0.0001 0.0001 0.0002 0.0001 0.0007 0 0.0013 0.0001 0.0006 0.0002 0.0007 0.0002 0.0006 0.0002
Peroxisomes 0.0043 0.0025 0.0009 0.0001 0.0054 0.001 0.0078 0.0011 0.004 0.0027 0.0025 0.0015 0.0092 0.0086 0.0097 0.0242 0.0052 0.0007
Punctate Nuclear 0 0 0 0 0 0.0001 0 0 0.0002 0 0.0025 0 0.0088 0 0.0007 0 0.0003 0
Vacuole 0.0002 0.0061 0.0003 0.0002 0.01 0.0004 0.0134 0.0083 0.003 0 0.0015 0.0009 0.001 0.0029 0.0032 0.0023 0.0028 0.0029
Vacuole Periphery 0.0034 0.0234 0.0056 0.0013 0.0381 0.0044 0.0058 0.0181 0.0024 0.0001 0.0078 0.0023 0.0036 0.0152 0.019 0.0037 0.0088 0.0356

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 50.8053 40.9391 51.2638 46.9298 45.4604 55.7833 54.3289 50.2007 50.5858 48.0865
Translational Efficiency 1.9426 2.2148 1.6715 2.3732 1.8577 1.4267 1.8441 2.2796 1.8842 1.8147

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1742 1082 1536 1510 1828 2103 1268 1054 3570 3185 2804 2564

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1228.45 1566.24 1467.38 1924.72 1241.37 1414.59 1628.95 1725.74 1235.07 1466.11 1540.44 1842.92
Standard Deviation 271.46 315.43 317.51 441.11 234.75 267.48 402.31 363.85 253.41 293.60 367.27 422.61
Intensity Change Log 2 0.350466 0.256403 0.647809 0.188451 0.392009 0.475282 0.271308 0.326153 0.563672

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001802 0.005358 0.001845 0.006472 0.001026 0.001249 0.004704 0.007984 0.001405 0.002645 0.003138 0.007093
Bud Neck 0.000914 0.002473 0.003952 0.001705 0.001379 0.000725 0.006893 0.002054 0.001152 0.001319 0.005282 0.001848
Bud Site 0.001121 0.001211 0.006255 0.003370 0.001449 0.001131 0.012888 0.002299 0.001289 0.001158 0.009255 0.002930
Cell Periphery 0.000443 0.000083 0.000556 0.000713 0.000055 0.000140 0.000694 0.000211 0.000244 0.000121 0.000618 0.000506
Cytoplasm 0.000206 0.000277 0.000821 0.000251 0.000165 0.000149 0.001432 0.000268 0.000185 0.000192 0.001098 0.000258
Cytoplasmic Foci 0.005710 0.026237 0.015926 0.008831 0.014215 0.007066 0.026966 0.014130 0.010065 0.013579 0.020918 0.011009
Eisosomes 0.000830 0.000894 0.000201 0.001016 0.000530 0.000399 0.000800 0.002161 0.000676 0.000567 0.000472 0.001487
Endoplasmic Reticulum 0.000109 0.000218 0.000173 0.000981 0.000160 0.000098 0.000256 0.000375 0.000135 0.000139 0.000210 0.000732
Endosome 0.001806 0.002830 0.009730 0.002182 0.002033 0.002507 0.007307 0.003888 0.001922 0.002617 0.008634 0.002883
Golgi 0.006921 0.026281 0.020779 0.031084 0.012273 0.012488 0.016713 0.032506 0.009661 0.017174 0.018940 0.031668
Lipid Particles 0.002076 0.006928 0.005275 0.004218 0.006205 0.004000 0.004063 0.006003 0.004190 0.004995 0.004727 0.004952
Mitochondria 0.963311* 0.896015* 0.874762* 0.894723* 0.938950* 0.950794* 0.849076* 0.902819* 0.950837* 0.932184* 0.863147* 0.898051*
Mitotic Spindle 0.000241 0.000714 0.006622 0.000756 0.000250 0.000336 0.003576 0.000742 0.000246 0.000464 0.005245 0.000751
None 0.001893 0.001878 0.001623 0.003457 0.002126 0.001409 0.001915 0.003114 0.002012 0.001568 0.001755 0.003316
Nuclear Periphery 0.000102 0.000415 0.001413 0.000661 0.000323 0.000168 0.000908 0.000535 0.000215 0.000252 0.001184 0.000609
Nuclear Periphery Foci 0.000114 0.000493 0.000951 0.000255 0.000365 0.000152 0.000908 0.000269 0.000243 0.000267 0.000931 0.000261
Nucleolus 0.000263 0.001253 0.001993 0.000815 0.000495 0.000586 0.002318 0.000353 0.000381 0.000813 0.002140 0.000625
Nucleus 0.000051 0.000279 0.000285 0.000116 0.000108 0.000149 0.000470 0.000072 0.000080 0.000194 0.000369 0.000098
Peroxisomes 0.006975 0.016401 0.008306 0.006016 0.010626 0.005465 0.017988 0.008010 0.008845 0.009180 0.012684 0.006836
Vacuole 0.001995 0.005515 0.018963 0.007276 0.002932 0.006446 0.021246 0.003384 0.002475 0.006129 0.019995 0.005676
Vacuole Periphery 0.003119 0.004248 0.019569 0.025102 0.004334 0.004543 0.018880 0.008824 0.003741 0.004443 0.019258 0.018410

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.86 -0.10 -4.31 -0.74 -4.56 -0.99 -4.96 -5.73 -5.52 -2.32 -2.61 -3.99 -7.14 -5.17 -4.69
Bud Neck -2.35 -6.36 -2.32 1.11 4.51 1.59 -6.21 -1.12 -2.65 5.16 -0.53 -8.52 -2.11 -1.57 6.87
Bud Site -0.21 -5.45 -2.45 -2.30 2.26 0.50 -6.16 -1.07 -1.68 5.62 0.33 -8.02 -2.55 -2.82 5.68
Cell Periphery 3.06 -0.38 -0.97 -2.48 -0.42 -2.14 -3.68 -4.65 -1.42 2.75 1.97 -2.09 -1.64 -2.54 0.50
Cytoplasm -1.04 -4.31 -0.66 0.46 4.21 0.49 -4.68 -2.07 -2.34 4.27 -0.21 -6.30 -1.73 -1.96 5.82
Cytoplasmic Foci -8.91 -6.92 -2.49 7.14 4.25 5.57 -5.02 0.04 -4.72 4.84 -3.21 -7.61 -0.91 2.10 6.56
Eisosomes -0.31 3.31 -0.89 -1.06 -9.68 2.45 -1.43 -5.92 -6.46 -4.16 1.08 1.62 -5.22 -7.26 -6.86
Endoplasmic Reticulum -1.78 -2.32 -2.26 -1.95 -2.09 1.34 -1.58 -2.04 -2.84 -1.13 -0.11 -2.32 -2.57 -2.55 -2.24
Endosome -2.34 -8.38 -0.80 1.27 7.71 -1.00 -5.60 -2.07 -1.55 2.84 -2.10 -9.99 -2.07 -0.57 7.63
Golgi -7.45 -6.22 -8.24 -1.30 -3.00 -0.13 -2.07 -5.97 -5.90 -4.34 -5.42 -5.91 -9.88 -6.07 -5.09
Lipid Particles -4.51 -4.19 -2.72 2.21 1.10 2.06 2.11 0.16 -1.67 -1.68 -1.09 -0.87 -1.06 0.05 -0.28
Mitochondria 9.14 13.17 10.80 0.17 -2.46 -2.42 10.80 4.93 6.86 -5.60 4.75 16.54 10.89 6.56 -5.60
Mitotic Spindle -2.88 -6.36 -2.88 -0.25 5.80 -0.71 -5.47 -2.67 -1.93 4.50 -2.15 -8.13 -3.98 -2.00 7.21
None 0.08 1.71 -7.43 -7.51 -9.84 5.45 1.63 -4.03 -7.29 -5.15 4.10 2.51 -8.30 -11.72 -10.76
Nuclear Periphery -2.73 -4.44 -7.25 -1.80 2.48 1.47 -3.29 -1.15 -2.15 1.68 -0.52 -5.33 -4.27 -3.72 3.00
Nuclear Periphery Foci -2.66 -3.96 -3.57 1.64 3.28 2.25 -2.04 0.89 -1.90 2.48 -0.36 -4.10 -0.33 0.11 4.09
Nucleolus -2.62 -3.90 -2.35 1.01 2.42 -0.50 -3.53 1.12 1.37 3.84 -2.46 -5.21 -1.65 0.88 4.25
Nucleus -1.54 -4.52 -2.26 1.09 2.99 -0.78 -3.37 1.04 1.62 3.77 -1.88 -5.09 -0.74 1.58 4.75
Peroxisomes -4.10 -0.92 0.61 4.55 1.58 3.26 -3.38 1.27 -1.40 4.24 -0.27 -2.99 1.59 1.83 4.43
Vacuole -3.71 -11.64 -6.21 -1.50 7.23 -3.59 -10.04 -0.72 2.97 9.66 -5.12 -15.24 -5.64 0.52 11.68
Vacuole Periphery -1.62 -11.12 -10.31 -9.83 -2.20 -0.28 -7.50 -4.15 -4.10 4.86 -1.34 -12.99 -10.94 -10.46 0.52
Standard name
Human Ortholog
Description Protein involved in Fe-S cluster transfer to mitochondrial clients; protects [4Fe-4S] clusters from damage due to oxidative stress; acts along with Bol3 at a late step in the transfer of [4Fe-4S] clusters from the ISA complex to client proteins; Fe-S loaded homodimer at steady state; similar to NifU, a bacterial protein required for Fe/S cluster maturation; ortholog of the human NFU1, mutations of which are associated with Multiple Mitochondria Dysfunctions Syndrome (MMDS1)
Localization
Cell Percentages mitochondrion (100%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Nfu1

Nfu1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nfu1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available