Standard name | |
---|---|
Human Ortholog | |
Description | Putative mannosidase; GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 1 | 16 | 10 | 4 | 3 | 8 | 8 | 0 | 1 | 1 | 0 | 6 | 6 | 3 | 0 | 2 | 2 | 1 | 7 | 9 | 0 | 2 | 3 |
Bud | 3 | 5 | 6 | 2 | 6 | 9 | 30 | 22 | 15 | 10 | 18 | 10 | 2 | 2 | 4 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 4 | 5 | 7 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 6 | 16 | 30 | 41 | 47 | 56 | 54 | 34 | 39 | 45 | 32 | 10 | 14 | 17 | 3 | 2 | 3 | 1 | 0 | 3 | 0 | 0 | 0 |
Cytoplasm | 48 | 124 | 52 | 113 | 201 | 284 | 317 | 393 | 225 | 247 | 260 | 255 | 91 | 92 | 127 | 37 | 58 | 45 | 8 | 26 | 12 | 25 | 32 | 25 |
Endoplasmic Reticulum | 56 | 113 | 204 | 78 | 130 | 87 | 99 | 92 | 39 | 33 | 25 | 44 | 165 | 191 | 184 | 0 | 0 | 4 | 74 | 124 | 192 | 85 | 109 | 125 |
Endosome | 0 | 0 | 8 | 4 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 6 | 6 | 6 | 0 | 0 | 0 | 2 | 4 | 23 | 6 | 12 | 11 |
Golgi | 0 | 0 | 6 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 6 | 1 | 3 | 1 | 6 | 0 | 1 | 3 | 0 | 1 | 1 |
Mitochondria | 0 | 1 | 0 | 1 | 0 | 1 | 2 | 1 | 1 | 2 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 2 |
Nucleus | 0 | 0 | 1 | 1 | 0 | 3 | 2 | 0 | 1 | 2 | 3 | 1 | 1 | 3 | 2 | 7 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 3 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 7 | 11 | 34 | 1 | 3 | 2 | 9 | 22 | 15 | 22 | 22 | 34 | 14 | 46 | 33 | 51 | 46 | 49 | 49 | 96 | 79 | 120 | 206 | 230 |
Unique Cell Count | 110 | 236 | 291 | 209 | 330 | 369 | 444 | 505 | 276 | 306 | 313 | 328 | 264 | 316 | 329 | 95 | 109 | 104 | 145 | 272 | 341 | 250 | 385 | 416 |
Labelled Cell Count | 120 | 261 | 345 | 244 | 385 | 437 | 527 | 594 | 330 | 358 | 376 | 380 | 298 | 368 | 378 | 101 | 113 | 113 | 145 | 272 | 341 | 250 | 385 | 416 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.4 | 18.2 | 14.4 | 14.3 | 14.9 | 14.2 | 13.8 | 14.2 | 13.8 | 13.7 | 13.4 | 13.6 | 16.3 | 16.1 | 15.5 | 21.9 | 20.2 | 22.2 | 14.9 | 14.9 | 15.9 |
Std Deviation (1e-4) | 2.0 | 3.1 | 2.3 | 2.2 | 2.2 | 2.1 | 2.2 | 2.3 | 2.4 | 2.5 | 2.3 | 3.0 | 2.7 | 2.5 | 2.6 | 4.6 | 4.6 | 4.5 | 2.2 | 2.5 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.05 | -0.02 | -0.06 | -0.02 | -0.06 | -0.07 | -0.11 | -0.09 | 0.18 | 0.16 | 0.1 | 0.6 | 0.49 | 0.62 | 0.05 | 0.05 | 0.14 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.4 | -3.0 | -3.6 | -2.8 | -3.1 | -4.0 | -3.8 | -3.8 | -4.3 | -1.9 | -2.4 | -3.3 | 0 | 0 | 0 |
Bud | 0 | -0.2 | 0.3 | 2.9 | 1.7 | 2.1 | 0.9 | 2.3 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 3.4 | 3.0 | 3.1 | 3.2 | 2.5 | 2.9 | 3.1 | 3.6 | 2.0 | -1.0 | -0.6 | -0.2 | 0 | 0 | -1.1 |
Cytoplasm | 8.5 | 10.9 | 15.1 | 14.2 | 16.4 | 15.2 | 15.4 | 16.0 | 14.9 | 4.5 | 3.3 | 5.7 | 4.2 | 7.0 | 5.2 |
Endoplasmic Reticulum | -7.3 | -7.7 | -12.0 | -12.9 | -14.6 | -13.5 | -14.8 | -15.7 | -14.4 | -1.9 | -2.5 | -3.6 | -11.9 | -12.5 | -11.6 |
Endosome | -0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.4 | -0.7 | -0.8 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.1 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.8 | -5.7 | -6.3 | -5.5 | -3.9 | -2.6 | -1.9 | -2.0 | -0.5 | -2.7 | 1.0 | -0.7 | 8.6 | 6.8 | 7.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 14.8347 | 14.4772 | 14.5945 | 13.7007 | 13.9958 | 14.171 | 12.6943 | 15.3616 | 14.7191 | 14.2923 | 12.7648 | 15.3925 | 13.2141 | 13.6951 | 13.8417 | 14.244 | 13.8045 | 13.7522 |
Actin | 0.0066 | 0.0028 | 0.0069 | 0.0294 | 0.0099 | 0.0037 | 0.0561 | 0.0003 | 0.0092 | 0.0005 | 0.0317 | 0.0005 | 0.0146 | 0.0004 | 0.0103 | 0.0001 | 0.0005 | 0.0027 |
Bud | 0.0005 | 0.0013 | 0.0122 | 0.0031 | 0.0023 | 0.0014 | 0.0091 | 0.0012 | 0.0179 | 0.0022 | 0.0067 | 0.0009 | 0.0008 | 0.001 | 0.0047 | 0.0008 | 0.0011 | 0.0007 |
Bud Neck | 0.0029 | 0.0043 | 0.0028 | 0.0016 | 0.0043 | 0.0177 | 0.0035 | 0.003 | 0.0047 | 0.0023 | 0.0016 | 0.0167 | 0.0019 | 0.0032 | 0.0022 | 0.0008 | 0.0014 | 0.012 |
Bud Periphery | 0.0029 | 0.0035 | 0.0644 | 0.0197 | 0.013 | 0.0116 | 0.0222 | 0.0036 | 0.0587 | 0.0093 | 0.0104 | 0.0046 | 0.0026 | 0.0026 | 0.0201 | 0.0062 | 0.0087 | 0.004 |
Bud Site | 0.0028 | 0.0272 | 0.0081 | 0.0015 | 0.0013 | 0.0015 | 0.0065 | 0.0122 | 0.0081 | 0.0015 | 0.004 | 0.001 | 0.0022 | 0.0132 | 0.003 | 0.0005 | 0.0007 | 0.0006 |
Cell Periphery | 0.1682 | 0.1262 | 0.1264 | 0.12 | 0.0812 | 0.1112 | 0.1059 | 0.1314 | 0.098 | 0.0693 | 0.1155 | 0.0971 | 0.1514 | 0.1703 | 0.1344 | 0.1339 | 0.1261 | 0.1046 |
Cytoplasm | 0.1121 | 0.1465 | 0.0976 | 0.0875 | 0.0564 | 0.0945 | 0.0595 | 0.108 | 0.075 | 0.0642 | 0.108 | 0.0812 | 0.0744 | 0.0446 | 0.1022 | 0.0488 | 0.0295 | 0.0735 |
Cytoplasmic Foci | 0.008 | 0.0082 | 0.0024 | 0.0029 | 0.0089 | 0.0049 | 0.0124 | 0.0027 | 0.0148 | 0.0084 | 0.0157 | 0.006 | 0.0142 | 0.003 | 0.007 | 0.0016 | 0.0031 | 0.0041 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.1115 | 0.1109 | 0.0962 | 0.0637 | 0.0417 | 0.0842 | 0.0666 | 0.0755 | 0.0442 | 0.0494 | 0.0177 | 0.0555 | 0.0779 | 0.0604 | 0.0965 | 0.0306 | 0.1148 | 0.107 |
Endosome | 0.0224 | 0.0401 | 0.0159 | 0.0269 | 0.0325 | 0.0251 | 0.0538 | 0.0146 | 0.0252 | 0.0437 | 0.0285 | 0.0249 | 0.0309 | 0.018 | 0.0241 | 0.026 | 0.0531 | 0.0213 |
Golgi | 0.0036 | 0.0044 | 0.0016 | 0.0012 | 0.0023 | 0.0017 | 0.0141 | 0.0007 | 0.0059 | 0.006 | 0.0356 | 0.0042 | 0.0044 | 0.0008 | 0.0071 | 0.0009 | 0.0027 | 0.0028 |
Lipid Particles | 0.0843 | 0.0633 | 0.0555 | 0.0357 | 0.0254 | 0.0894 | 0.066 | 0.0356 | 0.0376 | 0.091 | 0.0125 | 0.0664 | 0.0745 | 0.0569 | 0.0464 | 0.0473 | 0.026 | 0.0581 |
Mitochondria | 0.0006 | 0.0004 | 0.0006 | 0.0007 | 0.0006 | 0.0008 | 0.0075 | 0.0001 | 0.0021 | 0.0015 | 0.0433 | 0.0044 | 0.0013 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0013 |
None | 0.0048 | 0.0005 | 0.0003 | 0.0005 | 0.0013 | 0.001 | 0.0017 | 0.0002 | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0.0025 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0063 |
Nuclear Periphery | 0.0072 | 0.0071 | 0.0061 | 0.0057 | 0.0076 | 0.0077 | 0.0275 | 0.0076 | 0.0072 | 0.0054 | 0.0082 | 0.0063 | 0.0079 | 0.0131 | 0.008 | 0.0054 | 0.0071 | 0.0087 |
Nucleolus | 0.0007 | 0.001 | 0.0008 | 0.0004 | 0.0007 | 0.0007 | 0.0011 | 0.0014 | 0.0011 | 0.0059 | 0.0004 | 0.0008 | 0.0008 | 0.0013 | 0.0007 | 0.0005 | 0.0007 | 0.0013 |
Nucleus | 0.0023 | 0.0029 | 0.0028 | 0.0018 | 0.0029 | 0.0027 | 0.0071 | 0.0036 | 0.0042 | 0.0039 | 0.0043 | 0.0035 | 0.0023 | 0.0036 | 0.0027 | 0.0019 | 0.0019 | 0.0032 |
Peroxisomes | 0.0012 | 0.0001 | 0.0009 | 0.0026 | 0.0072 | 0.0002 | 0.0029 | 0.0001 | 0.0028 | 0.0002 | 0.0036 | 0.0001 | 0.0035 | 0.0001 | 0.0071 | 0 | 0 | 0.0003 |
Punctate Nuclear | 0.0025 | 0.0004 | 0.0003 | 0.0001 | 0.0046 | 0.0007 | 0.0013 | 0.0003 | 0.0005 | 0.0004 | 0.0019 | 0.0005 | 0.0027 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.001 |
Vacuole | 0.4513 | 0.4441 | 0.4879 | 0.5784 | 0.6757 | 0.5328 | 0.4608 | 0.5915 | 0.5675 | 0.6122 | 0.5414 | 0.6141 | 0.5224 | 0.6013 | 0.5127 | 0.6812 | 0.6058 | 0.5776 |
Vacuole Periphery | 0.0037 | 0.0047 | 0.0105 | 0.0153 | 0.0201 | 0.0064 | 0.0142 | 0.0064 | 0.0151 | 0.0223 | 0.0079 | 0.0112 | 0.0067 | 0.0058 | 0.0094 | 0.0131 | 0.0163 | 0.0089 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 45.3622 | 35.8018 | 45.0004 | 51.6216 | 57.7543 | 49.8299 | 52.0825 | 63.8224 | 67.5405 | 68.2657 |
Translational Efficiency | 2.2476 | 1.912 | 1.3717 | 1.3178 | 1.2048 | 2.1749 | 1.5301 | 0.9293 | 1.0682 | 1.1785 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1730 | 521 | 646 | 1243 | 2007 | 1617 | 691 | 569 | 3737 | 2138 | 1337 | 1812 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1221.56 | 1545.85 | 1639.11 | 1675.44 | 1317.75 | 1373.14 | 1610.34 | 1677.99 | 1273.22 | 1415.23 | 1624.24 | 1676.24 |
Standard Deviation | 199.97 | 243.94 | 212.94 | 278.78 | 189.32 | 197.63 | 220.19 | 284.09 | 200.16 | 222.57 | 217.19 | 280.46 |
Intensity Change Log 2 | — | 0.339676 | 0.424188 | 0.455815 | — | 0.059402 | 0.289289 | 0.348657 | — | 0.201033 | 0.355759 | 0.401201 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000658 | 0.001301 | 0.001083 | 0.004295 | 0.000345 | 0.001583 | 0.001688 | 0.006062 | 0.000490 | 0.001515 | 0.001395 | 0.004850 |
Bud Neck | 0.007338 | 0.011667 | 0.011559 | 0.008530 | 0.009000 | 0.020378 | 0.010092 | 0.006501 | 0.008231 | 0.018255 | 0.010801 | 0.007893 |
Bud Site | 0.026465 | 0.025221 | 0.024291 | 0.030367 | 0.012657 | 0.034847 | 0.012633 | 0.041922 | 0.019049 | 0.032501 | 0.018266 | 0.033996 |
Cell Periphery | 0.067408 | 0.224741* | 0.085244 | 0.575393* | 0.082180 | 0.156940* | 0.157343* | 0.567186* | 0.075341 | 0.173462* | 0.122507* | 0.572816* |
Cytoplasm | 0.049187 | 0.055292 | 0.010797 | 0.059797 | 0.035248 | 0.061062 | 0.039707 | 0.088689 | 0.041701 | 0.059656 | 0.025738 | 0.068870 |
Cytoplasmic Foci | 0.006240 | 0.006615 | 0.001924 | 0.003952 | 0.004997 | 0.011637 | 0.000489 | 0.003960 | 0.005573 | 0.010413 | 0.001182 | 0.003954 |
Eisosomes | 0.000071 | 0.000195 | 0.000123 | 0.000933 | 0.000080 | 0.000557 | 0.000564 | 0.001038 | 0.000076 | 0.000469 | 0.000351 | 0.000966 |
Endoplasmic Reticulum | 0.059084 | 0.112412* | 0.262095* | 0.075081 | 0.191645* | 0.225593* | 0.499233* | 0.074970 | 0.130278* | 0.198012* | 0.384655* | 0.075046 |
Endosome | 0.013710 | 0.012626 | 0.030186 | 0.005052 | 0.015531 | 0.038877 | 0.005811 | 0.004752 | 0.014688 | 0.032480 | 0.017588 | 0.004958 |
Golgi | 0.002299 | 0.002675 | 0.005456 | 0.005419 | 0.001690 | 0.006670 | 0.001293 | 0.007795 | 0.001972 | 0.005697 | 0.003304 | 0.006165 |
Lipid Particles | 0.003824 | 0.049737 | 0.030354 | 0.037539 | 0.011472 | 0.103727* | 0.021651 | 0.068168 | 0.007931 | 0.090570 | 0.025856 | 0.047157 |
Mitochondria | 0.000621 | 0.000270 | 0.000155 | 0.001002 | 0.000266 | 0.001019 | 0.000139 | 0.000279 | 0.000430 | 0.000836 | 0.000147 | 0.000775 |
Mitotic Spindle | 0.000053 | 0.000633 | 0.000163 | 0.003606 | 0.000038 | 0.000174 | 0.000072 | 0.001383 | 0.000045 | 0.000285 | 0.000116 | 0.002908 |
None | 0.000150 | 0.001751 | 0.000020 | 0.000079 | 0.000016 | 0.000049 | 0.000036 | 0.000141 | 0.000078 | 0.000463 | 0.000028 | 0.000098 |
Nuclear Periphery | 0.000091 | 0.000470 | 0.000405 | 0.006547 | 0.000167 | 0.000402 | 0.000783 | 0.003934 | 0.000132 | 0.000419 | 0.000601 | 0.005726 |
Nuclear Periphery Foci | 0.000878 | 0.003820 | 0.005865 | 0.003340 | 0.001241 | 0.003548 | 0.004474 | 0.003671 | 0.001073 | 0.003614 | 0.005146 | 0.003444 |
Nucleolus | 0.000422 | 0.000513 | 0.000109 | 0.000075 | 0.000263 | 0.000230 | 0.000044 | 0.000047 | 0.000336 | 0.000299 | 0.000075 | 0.000066 |
Nucleus | 0.001695 | 0.001782 | 0.000401 | 0.001360 | 0.000490 | 0.000764 | 0.000801 | 0.001210 | 0.001048 | 0.001012 | 0.000608 | 0.001313 |
Peroxisomes | 0.000714 | 0.001421 | 0.000315 | 0.002598 | 0.001174 | 0.005304 | 0.000557 | 0.002040 | 0.000961 | 0.004358 | 0.000440 | 0.002422 |
Vacuole | 0.713357* | 0.478686* | 0.515455* | 0.169887 | 0.616877* | 0.316793* | 0.241630* | 0.113609 | 0.661541* | 0.356244* | 0.373934* | 0.152214 |
Vacuole Periphery | 0.045737 | 0.008172 | 0.014001 | 0.005149 | 0.014622 | 0.009847 | 0.000959 | 0.002644 | 0.029026 | 0.009439 | 0.007260 | 0.004362 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.35 | -2.25 | -4.99 | -4.00 | -4.34 | -7.99 | -4.55 | -4.04 | -3.15 | -2.98 | -7.15 | -5.11 | -6.55 | -4.98 | -5.00 |
Bud Neck | -2.52 | -3.52 | -0.92 | 1.56 | 1.97 | -7.57 | -0.77 | 2.28 | 8.51 | 2.18 | -8.43 | -2.69 | 0.33 | 7.38 | 2.43 |
Bud Site | 0.26 | 0.29 | -1.18 | -1.09 | -1.18 | -7.94 | -0.18 | -5.80 | -1.29 | -5.42 | -5.64 | 0.02 | -5.35 | -0.50 | -4.67 |
Cell Periphery | -15.36 | -2.86 | -62.63* | -28.35* | -54.10* | -14.36 | -10.55 | -39.06* | -31.77* | -29.80* | -21.21 | -9.82 | -73.88 | -52.08* | -58.39* |
Cytoplasm | -0.97 | 11.27 | -2.35 | -0.66 | -12.47 | -7.28 | -1.22 | -7.73 | -3.88 | -6.55 | -5.79 | 5.72 | -7.47 | -2.27 | -11.34 |
Cytoplasmic Foci | -0.27 | 5.85 | 2.08 | 1.60 | -2.25 | -5.70 | 6.97 | 0.76 | 4.99 | -2.90 | -5.14 | 8.99 | 1.93 | 6.03 | -3.91 |
Eisosomes | -3.15 | -2.91 | -12.61 | -9.69 | -11.95 | -12.40 | -5.71 | -9.52 | -4.49 | -3.58 | -12.61 | -6.11 | -15.85 | -7.86 | -8.60 |
Endoplasmic Reticulum | -6.48 | -16.91 | -3.10 | 4.15 | 14.89 | -4.29 | -21.87 | 14.24 | 16.52 | 28.69* | -11.25 | -25.61 | 11.54 | 19.30 | 30.52 |
Endosome | 0.64 | -8.36 | 8.26 | 4.66 | 12.51 | -11.85 | 11.09 | 7.94 | 15.66 | 0.86 | -11.57 | -2.74 | 11.93 | 17.26 | 10.71 |
Golgi | -0.81 | -4.49 | -2.77 | -2.38 | -0.00 | -7.61 | 2.05 | -2.80 | -0.50 | -2.99 | -7.21 | -3.72 | -4.10 | -0.42 | -2.73 |
Lipid Particles | -6.70 | -8.12 | -11.83 | 1.64 | -1.63 | -18.16* | -4.13 | -8.53 | 4.30 | -6.70 | -19.80 | -8.78 | -13.44 | 8.63 | -6.12 |
Mitochondria | 1.25 | 1.68 | -1.19 | -4.14 | -4.92 | -6.23 | 4.29 | -0.27 | 5.90 | -3.04 | -2.60 | 2.18 | -2.00 | 0.40 | -5.27 |
Mitotic Spindle | -2.13 | -2.24 | -3.18 | -2.59 | -3.07 | -4.34 | -3.51 | -13.03 | -11.16 | -12.65 | -3.43 | -2.87 | -3.73 | -3.40 | -3.63 |
None | -0.94 | 1.62 | 0.88 | 0.98 | -3.41 | -3.61 | -1.64 | -1.47 | -1.08 | -1.19 | -0.92 | 1.28 | -0.43 | 0.88 | -2.32 |
Nuclear Periphery | -2.16 | -13.73 | -21.27 | -17.33 | -20.18 | -16.47* | -10.76 | -13.30 | -12.46 | -10.91 | -6.48 | -14.73 | -24.53 | -22.85 | -22.26 |
Nuclear Periphery Foci | -2.33 | -8.53 | -5.87 | 0.37 | 5.02 | -11.41 | -11.43 | -12.39 | -0.34 | 2.53 | -6.92 | -12.84 | -10.07 | 0.48 | 5.59 |
Nucleolus | -0.38 | 9.95 | 11.25 | 1.36 | 3.83 | 2.25 | 21.83* | 22.42* | 15.80 | -0.50 | 0.29 | 16.71 | 17.62 | 2.80 | 1.66 |
Nucleus | -0.17 | 1.68 | 0.44 | 0.44 | -10.30 | -4.96 | -3.66 | -8.88 | -4.85 | -3.08 | -0.01 | 1.14 | -0.77 | -0.79 | -8.69 |
Peroxisomes | -4.02 | 5.62 | -9.22 | -4.57 | -11.39 | -13.13 | 5.06 | -3.44 | 8.66 | -6.18 | -14.19 | 7.15 | -8.89 | 6.97 | -12.54 |
Vacuole | 17.30 | 15.00 | 73.74* | 23.75 | 27.49 | 34.79* | 35.40* | 65.28* | 24.92 | 12.68 | 42.25* | 31.14 | 93.18* | 29.93 | 24.99 |
Vacuole Periphery | 17.24* | 14.68 | 19.32 | 2.12 | 6.28 | 4.81 | 19.54 | 14.78 | 8.76 | -3.89 | 17.01 | 19.39 | 21.00 | 5.79 | 3.40 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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