Standard name | |
---|---|
Human Ortholog | |
Description | Serine/threonine protein kinase; regulates the orientation checkpoint, the morphogenesis checkpoint and the metabolic switch from fermentative to oxidative metabolism by phosphorylating the activation loop of Kin4p, Hsl1p and Snf4p respectively; cooperates with Hsl7p in recruiting Hsl1p to the septin ring, a prerequisite for subsequent recruitment, phosphorylation, and degradation of Swe1p; forms part of the bud neck ring; regulates cytokinesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 16 | 30 | 7 | 9 | 19 | 10 | 21 | – | 17 | 16 | 3 | 10 | 17 | 50 | 15 | 25 | 16 | 12 | 9 | 24 | 11 | 3 | 8 | 9 |
Bud | 14 | 23 | 2 | 9 | 29 | 18 | 37 | – | 29 | 19 | 26 | 26 | 2 | 15 | 7 | 17 | 7 | 12 | 21 | 29 | 20 | 2 | 3 | 4 |
Bud Neck | 22 | 30 | 21 | 27 | 56 | 27 | 52 | – | 34 | 33 | 25 | 22 | 24 | 73 | 61 | 65 | 46 | 36 | 60 | 107 | 56 | 10 | 12 | 12 |
Bud Site | 6 | 5 | 1 | 5 | 5 | 6 | 15 | – | 8 | 12 | 7 | 11 | 13 | 40 | 23 | 11 | 12 | 8 | – | – | – | – | – | – |
Cell Periphery | 6 | 30 | 7 | 16 | 7 | 6 | 13 | – | 13 | 20 | 19 | 17 | 4 | 18 | 30 | 74 | 61 | 44 | 2 | 9 | 6 | 5 | 19 | 14 |
Cytoplasm | 113 | 210 | 116 | 150 | 165 | 157 | 225 | – | 195 | 177 | 179 | 173 | 84 | 180 | 159 | 265 | 197 | 220 | 74 | 132 | 65 | 134 | 294 | 248 |
Endoplasmic Reticulum | 1 | 3 | 1 | 0 | 0 | 4 | 1 | – | 0 | 4 | 0 | 3 | 0 | 2 | 2 | 33 | 7 | 13 | 4 | 4 | 2 | 0 | 3 | 1 |
Endosome | 3 | 2 | 2 | 1 | 1 | 0 | 1 | – | 1 | 3 | 0 | 1 | 0 | 2 | 2 | 9 | 8 | 9 | 2 | 6 | 3 | 1 | 3 | 0 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 2 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 2 | 2 | 0 | 3 | 3 | 16 | 26 | – | 33 | 31 | 96 | 49 | 0 | 0 | 0 | 10 | 7 | 5 | 2 | 8 | 10 | 1 | 5 | 6 |
Nucleus | 0 | 1 | 2 | 0 | 8 | 17 | 5 | – | 3 | 3 | 2 | 3 | 0 | 5 | 5 | 9 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 6 | 4 | – | 0 | 1 | 2 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 3 | 0 | 2 | 3 | 1 | 1 | – | 4 | 1 | 3 | 3 | 0 | 7 | 42 | 9 | 12 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 9 | 15 | 23 | 32 | 76 | 26 | 61 | – | 17 | 37 | 12 | 34 | 22 | 83 | 118 | 80 | 68 | 62 | 2 | 6 | 5 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | 1 | 1 | 2 | 3 | 2 | 2 | – | 0 | 2 | 1 | 2 | 1 | 1 | 0 | 5 | 4 | 5 | 1 | 5 | 2 | 1 | 2 | 1 |
Unique Cell Count | 160 | 277 | 155 | 204 | 295 | 245 | 362 | 277 | 277 | 280 | 283 | 137 | 364 | 361 | 440 | 336 | 339 | 186 | 341 | 190 | 167 | 362 | 305 | |
Labelled Cell Count | 192 | 356 | 183 | 256 | 376 | 297 | 465 | 354 | 359 | 376 | 355 | 167 | 476 | 468 | 615 | 450 | 442 | 186 | 341 | 190 | 167 | 362 | 305 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.8 | 6.8 | 5.9 | 6.0 | 5.3 | 5.6 | – | 4.9 | 5.3 | 4.4 | 5.0 | 6.2 | 6.1 | 6.1 | 7.6 | 8.0 | 7.2 | 5.4 | 5.9 | 6.0 |
Std Deviation (1e-4) | 0.6 | 0.9 | 1.5 | 1.2 | 1.7 | 1.5 | 1.4 | – | 1.1 | 1.1 | 0.8 | 0.9 | 1.2 | 0.9 | 1.1 | 1.3 | 1.9 | 1.5 | 0.7 | 1.1 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.2 | -0.18 | -0.37 | -0.28 | – | -0.48 | -0.36 | -0.63 | -0.44 | -0.13 | -0.16 | -0.17 | 0.16 | 0.22 | 0.08 | -0.33 | -0.2 | -0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0.8 | -0.2 | 0.6 | – | 0.7 | 0.6 | 0 | -0.5 | 2.5 | 3.1 | -0.2 | 0.6 | 0.1 | -0.5 |
Bud | 0 | 3.4 | 2.7 | 3.5 | – | 3.5 | 2.6 | 0 | 3.2 | 0 | 1.7 | 0 | 0 | 0 | 0 |
Bud Neck | -0.1 | 1.5 | -0.8 | 0.2 | – | -0.4 | -0.5 | 0 | -1.9 | 0.9 | 1.8 | 1.0 | 0.4 | 0 | -0.9 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3.5 | 4.0 | 2.8 | 0 | 0 | 0 |
Cell Periphery | 1.3 | 0 | -1.1 | -0.5 | – | 0.1 | 1.1 | 0 | 0.7 | -0.7 | 0.2 | 1.5 | 3.8 | 4.1 | 2.9 |
Cytoplasm | -0.3 | -3.9 | -2.3 | -2.8 | – | -1.0 | -2.3 | -2.3 | -2.9 | -2.5 | -5.3 | -6.4 | -3.3 | -3.5 | -2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.2 | 3.4 | – | 4.5 | 4.3 | 0 | 5.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 2.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 4.4 | 0 | 0 | 0 |
SpindlePole | 0.2 | 2.7 | -1.3 | 0.6 | – | -3.0 | -0.4 | 0 | -0.8 | 0.3 | 2.1 | 4.2 | 0.9 | 1.4 | 0.9 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0276 | 0.3216 | 0.4508 | 0.2186 | 0.1531 | 0.0028 | 1.3885 | 0.9231 | 0.9878 | 0.4619 | 1.9522 | 1.1362 | 0.9466 | 0.8723 | 0.8539 | 1.4766 | 1.6025 | 0.9139 |
Actin | 0.0262 | 0.0093 | 0.0524 | 0.0001 | 0.0121 | 0.0147 | 0.0258 | 0.0014 | 0.0011 | 0.0014 | 0.0139 | 0.0075 | 0.0409 | 0.0028 | 0.0344 | 0.0542 | 0.0162 | 0.0102 |
Bud | 0.0011 | 0.0009 | 0.0055 | 0 | 0.0007 | 0.0005 | 0.003 | 0.0027 | 0.0004 | 0.0001 | 0.0112 | 0.0014 | 0.0038 | 0.0038 | 0.0008 | 0.0017 | 0.0005 | 0.0014 |
Bud Neck | 0.0303 | 0.0259 | 0.6137 | 0.9982 | 0.7057 | 0.2942 | 0.0384 | 0.0525 | 0.6993 | 0.9928 | 0.7558 | 0.2735 | 0.0445 | 0.0307 | 0.5865 | 0.827 | 0.5734 | 0.2428 |
Bud Periphery | 0.0024 | 0.0014 | 0.0032 | 0 | 0.0009 | 0.0021 | 0.0078 | 0.0034 | 0.0004 | 0.0002 | 0.0393 | 0.0093 | 0.0067 | 0.0038 | 0.0013 | 0.003 | 0.0008 | 0.0046 |
Bud Site | 0.167 | 0.2416 | 0.2021 | 0.0003 | 0.003 | 0.003 | 0.195 | 0.3559 | 0.2097 | 0.0038 | 0.0371 | 0.0021 | 0.2153 | 0.3063 | 0.2697 | 0.0468 | 0.0051 | 0.0089 |
Cell Periphery | 0.0261 | 0.0201 | 0.0082 | 0.0001 | 0.0006 | 0.0093 | 0.0357 | 0.0231 | 0.0025 | 0.0003 | 0.0054 | 0.0136 | 0.0242 | 0.0114 | 0.0031 | 0.0008 | 0.0038 | 0.0032 |
Cytoplasm | 0.3593 | 0.3965 | 0.0298 | 0.0002 | 0.0139 | 0.4716 | 0.2574 | 0.243 | 0.0161 | 0.0002 | 0.0167 | 0.3578 | 0.4175 | 0.4038 | 0.0535 | 0.0016 | 0.227 | 0.5513 |
Cytoplasmic Foci | 0.0199 | 0.0097 | 0.0124 | 0.0007 | 0.0395 | 0.0262 | 0.018 | 0.0056 | 0.0173 | 0.0004 | 0.0124 | 0.0054 | 0.0287 | 0.0228 | 0.0073 | 0.0043 | 0.011 | 0.0053 |
Eisosomes | 0.0028 | 0.0017 | 0.0015 | 0 | 0.0042 | 0.0007 | 0.0012 | 0.0007 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.0016 | 0.0004 | 0.0004 | 0.0005 | 0.0003 | 0.0003 |
Endoplasmic Reticulum | 0.0084 | 0.0045 | 0.0007 | 0 | 0.0005 | 0.0016 | 0.0174 | 0.0017 | 0.0007 | 0 | 0.0047 | 0.0063 | 0.0106 | 0.0027 | 0.0022 | 0.0044 | 0.0032 | 0.0035 |
Endosome | 0.0275 | 0.0012 | 0.0076 | 0 | 0.058 | 0.0006 | 0.0161 | 0.0021 | 0.0027 | 0 | 0.038 | 0.004 | 0.0196 | 0.0012 | 0.0044 | 0.0162 | 0.0173 | 0.0037 |
Golgi | 0.0161 | 0.001 | 0.0104 | 0 | 0.0406 | 0.0006 | 0.0034 | 0.0008 | 0.0002 | 0 | 0.0096 | 0.0008 | 0.0042 | 0.0005 | 0.0034 | 0.0061 | 0.0048 | 0.0009 |
Lipid Particles | 0.0278 | 0.0016 | 0.0046 | 0 | 0.024 | 0.0004 | 0.0059 | 0.0008 | 0.0002 | 0 | 0.0039 | 0.0007 | 0.0051 | 0.0012 | 0.003 | 0.0025 | 0.0085 | 0.0025 |
Mitochondria | 0.0161 | 0.0002 | 0.0036 | 0 | 0.0022 | 0.0002 | 0.0036 | 0.0002 | 0.0001 | 0 | 0.0037 | 0.0011 | 0.0019 | 0.0007 | 0.0005 | 0.004 | 0.001 | 0.001 |
None | 0.23 | 0.2694 | 0.0272 | 0 | 0.0702 | 0.1709 | 0.3308 | 0.2949 | 0.0264 | 0 | 0.0024 | 0.2886 | 0.1363 | 0.1988 | 0.0233 | 0.0017 | 0.0643 | 0.1479 |
Nuclear Periphery | 0.0074 | 0.0004 | 0.0004 | 0 | 0.0006 | 0.0001 | 0.0167 | 0.0004 | 0.0104 | 0 | 0.0099 | 0.0169 | 0.0147 | 0.0003 | 0.0006 | 0.0102 | 0.0111 | 0.0031 |
Nucleolus | 0.0061 | 0.001 | 0.0003 | 0.0001 | 0.0021 | 0.0002 | 0.0015 | 0.0014 | 0.0043 | 0.0003 | 0.0025 | 0.0003 | 0.0016 | 0.0005 | 0.0004 | 0.0017 | 0.0038 | 0.001 |
Nucleus | 0.0056 | 0.0059 | 0.0005 | 0.0001 | 0.0015 | 0.0006 | 0.0061 | 0.0023 | 0.0047 | 0.0002 | 0.0112 | 0.003 | 0.0075 | 0.0011 | 0.0007 | 0.0043 | 0.0065 | 0.0025 |
Peroxisomes | 0.0092 | 0.0002 | 0.0138 | 0 | 0.0134 | 0.0005 | 0.0016 | 0.0002 | 0.0002 | 0 | 0.0019 | 0.0002 | 0.002 | 0.0007 | 0.0013 | 0.0015 | 0.0021 | 0.0003 |
Punctate Nuclear | 0.0028 | 0.0013 | 0.0015 | 0.0001 | 0.0051 | 0.001 | 0.0085 | 0.0024 | 0.0019 | 0.0001 | 0.0085 | 0.0009 | 0.0059 | 0.0041 | 0.0028 | 0.004 | 0.0374 | 0.0034 |
Vacuole | 0.0063 | 0.0063 | 0.0007 | 0 | 0.0011 | 0.0009 | 0.0051 | 0.0044 | 0.0009 | 0.0001 | 0.0105 | 0.0046 | 0.0063 | 0.0023 | 0.0005 | 0.0022 | 0.0015 | 0.0018 |
Vacuole Periphery | 0.0018 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0011 | 0.0001 | 0.0002 | 0 | 0.0015 | 0.0015 | 0.0012 | 0.0001 | 0.0001 | 0.0015 | 0.0005 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.771 | 10.2587 | 4.1868 | 7.9387 | 11.5312 | 7.4552 | 9.5946 | 13.3015 | 9.0445 | 12.9039 |
Translational Efficiency | 0.4223 | 0.7585 | 0.9114 | 0.3704 | 0.4685 | 0.6598 | 0.6785 | 0.4276 | 0.4111 | 0.3533 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2214 | 1753 | 721 | 1493 | 1941 | 1844 | 1306 | 195 | 4155 | 3597 | 2027 | 1688 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 629.40 | 800.60 | 973.07 | 848.85 | 752.46 | 749.19 | 903.32 | 939.27 | 686.89 | 774.24 | 928.13 | 859.30 |
Standard Deviation | 71.63 | 97.87 | 112.70 | 106.14 | 83.68 | 99.13 | 98.56 | 103.18 | 98.87 | 101.81 | 109.05 | 109.68 |
Intensity Change Log 2 | — | 0.347104 | 0.628566 | 0.431532 | — | -0.006283 | 0.263622 | 0.319925 | — | 0.165461 | 0.441348 | 0.371832 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000620 | 0.001963 | 0.000794 | 0.003311 | 0.000599 | 0.001209 | 0.000830 | 0.006171 | 0.000610 | 0.001577 | 0.000817 | 0.003641 |
Bud Neck | 0.315456* | 0.219082* | 0.161549 | 0.172048 | 0.291781* | 0.224885* | 0.225704* | 0.177494 | 0.304396* | 0.222057* | 0.202884* | 0.172677 |
Bud Site | 0.116628* | 0.156393* | 0.181528* | 0.404940* | 0.118682* | 0.142364* | 0.310099* | 0.449446* | 0.117587* | 0.149201* | 0.264366* | 0.410081* |
Cell Periphery | 0.004518 | 0.000401 | 0.000440 | 0.000526 | 0.001266 | 0.000489 | 0.000422 | 0.000735 | 0.002999 | 0.000446 | 0.000429 | 0.000550 |
Cytoplasm | 0.359643* | 0.343766* | 0.237692* | 0.265986* | 0.374265* | 0.333140* | 0.322049* | 0.182376* | 0.366474* | 0.338319* | 0.292043* | 0.256327* |
Cytoplasmic Foci | 0.074297 | 0.112129 | 0.026523 | 0.017818 | 0.087347 | 0.112746 | 0.011165 | 0.021874 | 0.080394 | 0.112446 | 0.016628 | 0.018286 |
Eisosomes | 0.001737 | 0.000403 | 0.000131 | 0.000108 | 0.000431 | 0.000437 | 0.000071 | 0.000208 | 0.001127 | 0.000420 | 0.000092 | 0.000119 |
Endoplasmic Reticulum | 0.001094 | 0.000535 | 0.004534 | 0.000433 | 0.000388 | 0.000421 | 0.000292 | 0.001491 | 0.000764 | 0.000476 | 0.001801 | 0.000555 |
Endosome | 0.007523 | 0.020966 | 0.012031 | 0.004806 | 0.010870 | 0.036757 | 0.005699 | 0.002751 | 0.009087 | 0.029061 | 0.007952 | 0.004569 |
Golgi | 0.003136 | 0.005633 | 0.000495 | 0.001703 | 0.001993 | 0.007032 | 0.000883 | 0.000752 | 0.002602 | 0.006350 | 0.000745 | 0.001593 |
Lipid Particles | 0.003648 | 0.001853 | 0.002623 | 0.000382 | 0.001973 | 0.003662 | 0.000289 | 0.000810 | 0.002866 | 0.002780 | 0.001120 | 0.000431 |
Mitochondria | 0.004596 | 0.004620 | 0.000474 | 0.002108 | 0.001188 | 0.006900 | 0.001586 | 0.001583 | 0.003004 | 0.005789 | 0.001190 | 0.002048 |
Mitotic Spindle | 0.000586 | 0.004178 | 0.005845 | 0.015720 | 0.001203 | 0.007148 | 0.011101 | 0.007946 | 0.000874 | 0.005701 | 0.009232 | 0.014822 |
None | 0.005865 | 0.000454 | 0.001383 | 0.000564 | 0.001641 | 0.000893 | 0.000624 | 0.000321 | 0.003892 | 0.000679 | 0.000894 | 0.000536 |
Nuclear Periphery | 0.000072 | 0.000065 | 0.001052 | 0.000134 | 0.000068 | 0.000221 | 0.000153 | 0.000163 | 0.000070 | 0.000145 | 0.000473 | 0.000137 |
Nuclear Periphery Foci | 0.000265 | 0.000242 | 0.003138 | 0.001037 | 0.000354 | 0.000309 | 0.000291 | 0.001103 | 0.000306 | 0.000276 | 0.001304 | 0.001045 |
Nucleolus | 0.001308 | 0.000319 | 0.001219 | 0.000208 | 0.000448 | 0.000803 | 0.000206 | 0.000176 | 0.000907 | 0.000567 | 0.000566 | 0.000205 |
Nucleus | 0.069851 | 0.026743 | 0.191956* | 0.022453 | 0.051100 | 0.020153 | 0.029887 | 0.016715 | 0.061092 | 0.023365 | 0.087535 | 0.021790 |
Peroxisomes | 0.003219 | 0.013825 | 0.009600 | 0.010751 | 0.004285 | 0.014017 | 0.005891 | 0.008667 | 0.003717 | 0.013923 | 0.007210 | 0.010510 |
Vacuole | 0.025193 | 0.084850 | 0.156643 | 0.074113 | 0.049731 | 0.084486 | 0.072012 | 0.119135 | 0.036656 | 0.084663 | 0.102115 | 0.079314 |
Vacuole Periphery | 0.000744 | 0.001580 | 0.000349 | 0.000851 | 0.000386 | 0.001930 | 0.000745 | 0.000085 | 0.000577 | 0.001759 | 0.000604 | 0.000762 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.21 | -3.01 | -6.47 | -2.48 | -5.53 | -3.61 | -1.76 | -3.03 | -2.70 | -2.92 | -5.42 | -2.96 | -7.16 | -4.49 | -6.48 |
Bud Neck | 9.68 | 19.48 | 20.03 | 9.75 | -2.64 | 6.20 | 9.31 | 10.94 | 6.92 | 4.74 | 11.27 | 18.63 | 23.54 | 12.98 | 4.31 |
Bud Site | -5.87 | -0.25 | -21.21 | -16.15 | -16.65 | -3.71 | -16.12 | -8.67 | -7.65 | -2.32 | -6.77 | -14.14 | -23.45 | -19.32 | -10.02 |
Cell Periphery | 9.18 | 9.19 | 9.04 | -1.37 | -1.52 | 6.17 | 7.04 | 3.32 | -1.15 | -1.82 | 10.31 | 10.52 | 10.13 | -0.88 | -2.26 |
Cytoplasm | 1.35 | 11.71 | 5.40 | 3.93 | -6.41 | 3.95 | 1.95 | 9.43 | 7.38 | 7.90 | 3.75 | 6.99 | 8.56 | 5.49 | 1.55 |
Cytoplasmic Foci | -8.41 | 17.88 | 19.48 | 22.65 | 1.10 | -5.30 | 23.64 | 11.78 | 15.43 | -3.56 | -9.80 | 30.70 | 25.93 | 31.06 | -3.71 |
Eisosomes | 9.53 | 12.83 | 13.01 | 4.28 | 2.54 | -0.05 | 14.36 | 3.51 | 2.44 | -2.78 | 8.58 | 15.12 | 14.56 | 6.01 | -3.48 |
Endoplasmic Reticulum | 2.23 | -10.71 | 1.53 | -1.80 | 12.21 | -0.43 | -1.05 | -4.32 | -4.26 | -4.18 | 2.10 | -8.93 | -1.35 | -5.01 | 8.77 |
Endosome | -9.27 | -2.30 | 4.61 | 11.84 | 5.75 | -12.60 | 5.76 | 8.68 | 17.00 | 2.29 | -15.70 | 3.20 | 7.91 | 19.74 | 4.08 |
Golgi | -4.33 | 12.24 | 4.30 | 6.73 | -2.36 | -8.81 | 4.75 | 5.37 | 11.96 | 0.98 | -9.27 | 11.12 | 3.82 | 10.30 | -1.69 |
Lipid Particles | 5.38 | 0.73 | 10.33 | 6.88 | 12.76 | -4.01 | 11.05 | 2.19 | 4.95 | -5.00 | 0.26 | 7.43 | 12.39 | 8.98 | 7.73 |
Mitochondria | 0.07 | 7.01 | 3.48 | 3.17 | -1.65 | -5.66 | -0.38 | 0.03 | 4.48 | 0.24 | -4.04 | 4.25 | 2.15 | 5.06 | -0.76 |
Mitotic Spindle | -3.57 | -5.12 | -7.14 | -4.71 | -2.41 | -4.65 | -4.82 | -3.21 | -1.22 | -0.23 | -5.93 | -6.63 | -7.49 | -4.32 | -1.92 |
None | 7.05 | 5.49 | 6.29 | -1.92 | 2.64 | 2.13 | 2.53 | 2.96 | 1.38 | 1.03 | 7.26 | 6.34 | 6.44 | -0.78 | 1.11 |
Nuclear Periphery | 0.66 | -15.95 | -6.63 | -6.77 | 14.16 | -1.53 | -9.11 | -3.06 | -1.80 | -1.75 | -1.48 | -16.76 | -7.13 | -2.31 | 10.55 |
Nuclear Periphery Foci | 0.23 | -9.44 | -1.61 | -1.64 | 4.86 | 0.49 | -0.84 | -3.59 | -3.67 | -3.37 | 0.44 | -8.89 | -1.94 | -1.99 | 1.30 |
Nucleolus | 4.12 | 0.14 | 4.32 | 1.01 | 6.00 | -1.90 | 3.18 | 0.46 | 1.70 | -0.48 | 2.01 | 2.08 | 4.58 | 2.77 | 4.35 |
Nucleus | 15.57 | -16.48 | 7.74 | -5.78 | 19.04 | 13.13 | 1.78 | 1.87 | -3.02 | 0.88 | 20.50 | -11.10 | 6.59 | -7.85 | 14.15 |
Peroxisomes | -10.62 | -3.13 | -5.43 | 3.55 | -1.93 | -8.49 | -0.95 | -2.13 | 3.78 | -1.41 | -13.47 | -2.73 | -5.26 | 4.56 | -2.86 |
Vacuole | -19.58 | -22.10 | -20.91 | -9.90 | 7.43 | -9.63 | -10.80 | -9.36 | -7.79 | -6.57 | -20.53 | -23.34 | -21.19 | -12.60 | -1.39 |
Vacuole Periphery | -1.96 | 4.62 | 0.40 | 1.93 | -1.18 | -7.53 | -0.83 | 8.50 | 9.03 | 1.84 | -5.13 | 0.27 | 0.00 | 3.72 | -0.18 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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