Standard name
Human Ortholog
Description Centromeric histone H3-like protein; associates with promoters, accessible chromatin, and RNAPII-bound regions; phosphorylated Cse4p associates with centromeres; required for kinetochore function; Ipl1p-dependent phosphorylation destabilizes defective kinetochores promoting bi-orientation; increases association of Sgo1p with centromeric chromatin; proteolysis regulated by multiple E3 ligases, resulting in faithful chromosome segregation; CSE4 complements mutations in the human homolog CENPA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.06 – 0 0 0 0 – 0.05 0 0 0 0.07 0 0 0 0 0 0.06 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0.09 – 0.06 0.05 0 0 – 0.09 0.06 0.14 0.07 0.1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 – 0.14 0.09 0.2 0.23 – 0.16 0.14 0.06 0 0.07 0.15 0.24 0.25 0.15 0.26 0.44 0.07 0 0 0.05 0.05 0.06
Endoplasmic Reticulum 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.12 0.06 0 0 0 0 0 0
Endosome 0.2 – 0 0.05 0.27 0.09 – 0.09 0 0 0 0 0.25 0.27 0.36 0.34 0.19 0.19 0.1 0.18 0.13 0.07 0.16 0.14
Golgi 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 – 0.49 0.37 0.1 0.3 – 0.31 0.44 0.42 0.52 0.52 0 0.05 0 0.1 0.1 0.06 0 0.05 0.08 0 0 0.06
Nucleus 0.09 – 0 0.05 0.1 0.05 – 0.06 0.06 0.09 0.07 0 0.1 0.05 0 0.08 0.31 0.06 0.1 0.09 0.06 0.07 0.09 0.07
Nuclear Periphery 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.4 – 0.21 0.51 0.26 0.34 – 0.23 0.29 0.3 0.27 0.22 0.31 0.23 0.18 0.38 0.19 0.19 0 0 0 0.05 0.05 0
Peroxisomes 0.09 – 0 0.08 0.11 0.05 – 0 0.06 0.06 0.09 0.07 0.1 0.07 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.2 – 0.37 0.4 0.4 0.42 – 0.46 0.38 0.52 0.44 0.44 0.51 0.38 0.43 0.41 0.26 0.19 0.5 0.23 0.49 0.57 0.4 0.39
Vac/Vac Membrane 0.09 – 0 0.05 0.08 0 – 0 0 0 0 0 0.05 0.05 0.1 0.11 0.1 0.19 0.11 0.38 0.07 0.05 0.13 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 1 3
Bud 2 – 3 1 1 0 – 10 4 3 3 12 0 1 0 3 0 1 1 0 0 2 2 9
Bud Neck 0 – 2 0 3 0 – 8 0 2 0 2 1 3 1 0 1 0 0 0 1 0 0 2
Bud Site 3 – 5 4 0 5 – 20 7 14 9 17 2 3 5 2 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 – 1 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 – 12 8 26 30 – 35 15 6 5 12 25 63 61 11 11 7 5 0 1 7 14 17
Endoplasmic Reticulum 0 – 2 0 0 0 – 0 0 0 1 0 0 2 0 1 5 1 0 0 0 0 1 2
Endosome 7 – 3 4 36 12 – 19 4 1 3 4 41 69 87 25 8 3 7 5 11 10 40 41
Golgi 0 – 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 1 4
Mitochondria 7 – 43 32 13 39 – 69 48 41 63 86 4 13 5 7 4 1 0 1 7 5 8 16
Nucleus 3 – 3 4 13 6 – 13 7 9 8 7 17 14 6 6 13 1 7 2 5 10 23 18
Nuclear Periphery 1 – 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2
Nucleolus 14 – 18 44 34 44 – 51 31 29 33 36 51 60 43 28 8 3 3 0 1 7 11 9
Peroxisomes 3 – 2 7 14 7 – 9 6 6 11 11 16 18 10 0 0 0 1 0 1 1 1 1
SpindlePole 7 – 32 35 53 54 – 102 41 51 54 73 83 98 103 30 11 3 38 6 45 84 101 112
Vac/Vac Membrane 3 – 3 4 10 2 – 3 0 0 1 5 8 14 24 8 4 3 8 11 6 7 33 36
Unique Cell Count 35 87 87 132 130 220 108 98 122 166 162 258 241 73 42 16 77 29 92 149 256 289
Labelled Cell Count 53 128 144 203 199 340 163 162 191 265 249 360 345 121 65 23 77 29 92 149 256 289


Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 – 4.2 4.1 5.2 4.2 – 3.9 3.6 3.7 3.3 3.7 5.1 5.3 5.0 6.8 5.4 6.4 5.4 5.6 5.4
Std Deviation (1e-4) 0.7 – 1.7 1.7 2.5 2.3 – 1.7 2.3 2.1 0.9 2.2 1.5 1.8 1.8 1.9 1.3 1.6 2.3 2.1 2.2
Intensity Change (Log2) – – – -0.01 0.31 -0.0 – -0.11 -0.21 -0.16 -0.36 -0.17 0.3 0.34 0.25 0.71 0.38 0.63 0.38 0.41 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.0 1.1 1.7 – 0.5 0 -1.8 -2.5 -1.7 0.3 2.1 2.2 0.2 1.7 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 4.5 0 – 0 0 0 0 0 4.3 4.6 5.9 5.1 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -6.6 0 – 0 0 0 0 0 -9.0 -9.7 -10.7 -5.4 -4.4 -3.2
Nucleus 0 1.8 0 – 0 0 1.6 0 0 2.0 0 0 0 4.4 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.9 0 – 0 0 0 0 0 1.8 0.5 -0.6 2.5 -0.2 0
Peroxisomes 0 2.3 0 – 0 0 0 0 0 2.2 1.6 0 0 0 0
SpindlePole 0 0.5 0 – 0 0 0 0 0 2.2 0.2 1.0 0.6 -1.2 -1.4
Vacuole 0 1.3 0 – 0 0 0 0 0 0 0 1.9 1.9 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1129 -0.0032 0.1683 -0.2526 -0.4052 -0.2088 -0.0276 -0.6298 -0.6418 -0.3186 -0.6833 -0.399 1.5706 0.6663 0.8079 1.1412 1.3853 0.3577
Actin 0.0702 0.0147 0.0113 0.0351 0.0011 0.0008 0.0016 0.0004 0.0109 0.0175 0.0033 0.0059 0.0126 0.0012 0.0032 0.0006 0.0494 0.0069
Bud 0.0015 0.0012 0.0003 0.0002 0.0002 0.0001 0.0004 0.0039 0.0004 0.0002 0.0025 0.0001 0.0007 0.0006 0.0002 0 0.0006 0.0003
Bud Neck 0.0043 0.0012 0.0013 0.0016 0.0043 0.0029 0.0041 0.0044 0.0011 0.0029 0.0017 0.0075 0.0007 0.0018 0.0047 0.0147 0.0005 0.0052
Bud Periphery 0.0017 0.0015 0.0002 0.0002 0.0004 0.0002 0.0004 0.0008 0.0004 0.0002 0.0041 0.0003 0.0013 0.001 0.0002 0 0.0007 0.0008
Bud Site 0.0146 0.0061 0.002 0.0007 0.0025 0.0006 0.0082 0.0116 0.0028 0.0039 0.0191 0.0007 0.0021 0.0136 0.004 0.0002 0.0028 0.0007
Cell Periphery 0.0006 0.0003 0.0001 0.0001 0.0002 0.0001 0.0009 0.0003 0.0001 0.0006 0.0006 0.0002 0.0004 0.0002 0.0002 0 0.0001 0.0002
Cytoplasm 0.0371 0.1249 0.0827 0.0367 0.0653 0.0313 0.047 0.0728 0.0315 0.0335 0.0209 0.028 0.0738 0.0539 0.0162 0.0197 0.0086 0.0418
Cytoplasmic Foci 0.1054 0.0422 0.0596 0.036 0.0579 0.0266 0.0789 0.0557 0.0468 0.0767 0.0889 0.0474 0.0726 0.0354 0.0445 0.0357 0.1479 0.0347
Eisosomes 0.0004 0.0002 0 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.001 0.0001 0.0001 0.0003 0.0001 0.0001 0 0.0002 0.0001
Endoplasmic Reticulum 0.0077 0.008 0.0065 0.0029 0.0045 0.0012 0.0074 0.007 0.0016 0.0025 0.0016 0.0065 0.0124 0.0029 0.0019 0.0004 0.0004 0.002
Endosome 0.0469 0.0177 0.0249 0.0294 0.0785 0.0101 0.1374 0.0221 0.0183 0.0483 0.0906 0.0345 0.0576 0.0134 0.0197 0.0042 0.07 0.0174
Golgi 0.0187 0.0031 0.0063 0.0102 0.0068 0.0028 0.0102 0.0036 0.0026 0.0066 0.032 0.0086 0.0074 0.0016 0.0045 0.0005 0.0081 0.0022
Lipid Particles 0.0472 0.0174 0.0142 0.0123 0.0228 0.0129 0.0398 0.0061 0.0113 0.0146 0.0456 0.0346 0.0105 0.015 0.0141 0.0022 0.0338 0.0156
Mitochondria 0.017 0.0053 0.0019 0.0109 0.0044 0.0006 0.0028 0.0007 0.0087 0.0015 0.0136 0.0123 0.01 0.0009 0.0009 0.0003 0.0185 0.019
None 0.0328 0.2017 0.1022 0.136 0.0823 0.1093 0.1468 0.1762 0.0899 0.1528 0.0733 0.0478 0.3085 0.1978 0.0823 0.1757 0.1328 0.055
Nuclear Periphery 0.0366 0.0324 0.046 0.0287 0.0233 0.0388 0.0407 0.0297 0.0409 0.0623 0.0114 0.0148 0.049 0.029 0.0286 0.0171 0.0024 0.0177
Nucleolus 0.0059 0.0112 0.009 0.0065 0.0305 0.0175 0.0565 0.0135 0.0109 0.0119 0.0103 0.0333 0.0027 0.0188 0.0213 0.008 0.0059 0.0271
Nucleus 0.1176 0.129 0.1797 0.1508 0.1286 0.1315 0.1199 0.1455 0.2021 0.0543 0.0132 0.1716 0.0424 0.2356 0.1611 0.1198 0.0027 0.1451
Peroxisomes 0.0253 0.0087 0.0042 0.0033 0.0082 0.0025 0.0079 0.002 0.0113 0.0331 0.0113 0.0091 0.0019 0.006 0.0083 0.002 0.0113 0.0019
Punctate Nuclear 0.4025 0.3689 0.4441 0.4937 0.4625 0.6082 0.2617 0.4327 0.5064 0.473 0.5451 0.5279 0.3225 0.369 0.5766 0.5981 0.4948 0.6009
Vacuole 0.0042 0.0034 0.0025 0.0029 0.0136 0.0018 0.0244 0.01 0.0011 0.0019 0.0093 0.0078 0.0071 0.0019 0.0069 0.0004 0.0055 0.0045
Vacuole Periphery 0.0017 0.0008 0.001 0.0016 0.0019 0.0004 0.0031 0.0009 0.0008 0.0007 0.0015 0.0011 0.0034 0.0004 0.0007 0.0003 0.003 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.5819 14.2953 5.3039 9.7694 30.0636 17.5808 16.9834 13.6576 11.7122 19.8942
Translational Efficiency 1.7335 3.4601 4.1683 2.3793 1.5708 3.2829 2.1719 2.2118 2.0744 2.217

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Centromeric histone H3-like protein; associates with promoters, accessible chromatin, and RNAPII-bound regions; phosphorylated Cse4p associates with centromeres; required for kinetochore function; Ipl1p-dependent phosphorylation destabilizes defective kinetochores promoting bi-orientation; increases association of Sgo1p with centromeric chromatin; proteolysis regulated by multiple E3 ligases, resulting in faithful chromosome segregation; CSE4 complements mutations in the human homolog CENPA
Localization
Cell Percentages spindle pole (39%), nucleus (18%), mixed (15%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cse4

Cse4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cse4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available