Standard name
Human Ortholog
Description Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase; may comprise a type II mitochondrial fatty acid synthase along with Mct1p; human homolog CBR4 complements yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.52 0.71 0.61 0.61 0.56 0.39 0.44 0.4 0.27 0.22 0.23 0.16 0.75 0.78 0.8 – 0.42 0.45 0.19 0.36 0.19 0.42 0.43 0.34
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.1 0 0 0 0 0 0
Endosome 0 0 0.07 0.05 0.12 0.1 0.1 0.1 0 0 0 0 0.08 0.05 0.05 – 0.24 0.22 0.11 0.07 0.08 0.06 0 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.27 0.22 0 0.16 0 0.33 0.38 0.28 0.73 0.67 0.74 0.75 0.08 0 0 – 0.11 0 0.44 0.28 0.39 0.28 0.21 0.26
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.11 0 0 0 0 0 0 0
SpindlePole 0.16 0.08 0.28 0.18 0.23 0.13 0.12 0.19 0.07 0.13 0.08 0.11 0.09 0.13 0.12 – 0.3 0.22 0.15 0.17 0.19 0.1 0.14 0.12
Vac/Vac Membrane 0 0 0 0 0.1 0.1 0.09 0.09 0.05 0.05 0 0 0 0 0 – 0.1 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 10 5 3 4 3 1 1 0 1 0 4 1 4 0 – 0 3 0 4 12 3 5 13
Bud 2 4 10 4 8 10 19 13 22 29 20 25 3 6 5 – 2 4 0 0 3 3 11 9
Bud Neck 1 3 12 4 7 7 7 11 2 3 3 3 1 6 4 – 0 0 1 3 3 1 4 5
Bud Site 0 3 1 3 0 4 7 0 4 3 9 8 0 1 0 – 0 2 – – – – – –
Cell Periphery 1 0 3 3 1 3 3 3 2 6 3 3 0 3 2 – 1 1 0 0 1 0 0 2
Cytoplasm 32 118 158 112 189 126 264 199 142 155 129 79 88 217 296 – 41 56 19 66 63 87 121 113
Endoplasmic Reticulum 2 2 1 4 3 7 1 2 1 3 2 3 1 1 2 – 3 13 1 0 2 1 1 7
Endosome 1 1 18 9 40 33 60 52 8 21 4 16 9 13 20 – 23 28 11 12 27 11 12 23
Golgi 0 0 0 2 0 0 0 3 0 0 0 0 0 0 0 – 0 1 0 0 3 4 2 4
Mitochondria 17 37 5 30 15 109 224 142 387 468 407 370 10 2 1 – 11 5 45 52 128 59 58 86
Nucleus 0 1 0 1 0 6 10 17 9 13 15 7 0 1 0 – 0 2 0 0 0 0 2 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 4 1 0 0 0 1 – 0 0 1 0 1 0 3 1
Nucleolus 0 0 0 0 0 2 2 1 0 2 2 3 0 0 1 – 0 0 0 0 0 0 1 1
Peroxisomes 0 1 2 1 10 9 10 5 3 3 2 10 2 9 11 – 11 4 1 1 8 1 1 1
SpindlePole 10 14 72 34 78 44 69 94 35 87 46 56 11 37 43 – 29 27 15 30 62 21 40 39
Vac/Vac Membrane 1 1 4 1 35 33 51 45 26 36 16 17 3 1 7 – 10 18 0 6 3 6 9 13
Unique Cell Count 62 166 260 184 338 326 595 500 528 694 550 492 118 277 369 97 125 102 185 333 210 283 334
Labelled Cell Count 69 195 291 211 390 396 728 588 641 834 659 604 129 301 393 131 164 102 185 333 210 283 334


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 3.4 5.4 5.1 5.7 4.6 4.4 4.9 3.8 4.0 3.7 3.9 4.7 5.0 5.0 4.4 5.7 5.6 6.5 6.7 6.3
Std Deviation (1e-4) 0.7 0.5 1.5 1.8 1.5 1.5 1.4 1.7 1.3 0.8 1.0 0.8 0.9 1.0 1.3 1.1 2.1 1.5 3.2 3.1 2.4
Intensity Change (Log2) – – – -0.09 0.06 -0.24 -0.31 -0.13 -0.52 -0.45 -0.54 -0.48 -0.22 -0.13 -0.12 -0.29 0.07 0.06 0.26 0.31 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 5.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.391 4.5011 4.5544 4.1323 4.3162 4.3062 4.0758 3.6133 3.2994 3.4669 2.5134 3.0548 -0.2743 0.3634 0.0374 0.0256 -0.2122 0.3489
Actin 0.062 0.0461 0.0287 0.0152 0.0163 0.0178 0.0933 0.037 0.0424 0.001 0.0073 0.0165 0.0606 0.0418 0.0402 0.057 0.0026 0.0146
Bud 0.0022 0.0188 0.0082 0.0139 0.0099 0.0024 0.0027 0.0077 0.0038 0.0021 0.0025 0.004 0.002 0.0043 0.0033 0.0089 0.0055 0.001
Bud Neck 0.0027 0.0018 0.0034 0.0039 0.0035 0.0155 0.0149 0.004 0.0143 0.0047 0.002 0.0199 0.0075 0.0032 0.0079 0.0075 0.0012 0.0275
Bud Periphery 0.0044 0.0153 0.0139 0.0403 0.0249 0.0055 0.0051 0.0092 0.0053 0.0093 0.0071 0.0098 0.0037 0.0046 0.0057 0.0111 0.0058 0.0018
Bud Site 0.0136 0.0562 0.018 0.0125 0.0387 0.0016 0.027 0.11 0.0388 0.0031 0.0019 0.0055 0.017 0.0487 0.0154 0.0082 0.0029 0.0017
Cell Periphery 0.0034 0.003 0.0051 0.002 0.0026 0.0017 0.0024 0.0041 0.0018 0.0018 0.0018 0.0043 0.0025 0.0017 0.0071 0.0011 0.0004 0.0007
Cytoplasm 0.0814 0.1518 0.1568 0.2035 0.1633 0.1478 0.0869 0.159 0.2562 0.2622 0.1789 0.2841 0.1572 0.1869 0.2597 0.2568 0.1795 0.1478
Cytoplasmic Foci 0.1152 0.1083 0.0984 0.0706 0.1038 0.1296 0.1081 0.1211 0.0881 0.043 0.1749 0.1158 0.1463 0.1486 0.1494 0.1003 0.1681 0.2065
Eisosomes 0.0021 0.0014 0.0016 0.0004 0.0003 0.0007 0.0013 0.0007 0.001 0.0003 0.0005 0.0006 0.0006 0.0006 0.0018 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0118 0.0047 0.005 0.0044 0.0078 0.0045 0.0158 0.0061 0.0108 0.0033 0.0059 0.0137 0.009 0.0039 0.0083 0.0043 0.0044 0.003
Endosome 0.1676 0.1281 0.1139 0.072 0.1387 0.1284 0.1241 0.1184 0.1023 0.0308 0.0927 0.0961 0.1786 0.13 0.1222 0.1234 0.1455 0.1983
Golgi 0.0982 0.0778 0.0788 0.0302 0.0894 0.0675 0.058 0.0835 0.0361 0.0128 0.0476 0.0633 0.0677 0.0978 0.0612 0.0543 0.0412 0.0811
Lipid Particles 0.0373 0.0073 0.0091 0.0104 0.0031 0.0143 0.0899 0.0108 0.0079 0.0123 0.0124 0.0216 0.0252 0.0192 0.0166 0.0055 0.066 0.025
Mitochondria 0.1965 0.2142 0.2741 0.3883 0.2464 0.3158 0.1059 0.1596 0.1847 0.2938 0.2021 0.1853 0.1999 0.1986 0.1695 0.2747 0.2201 0.1951
None 0.1271 0.1324 0.12 0.0657 0.0329 0.0803 0.1265 0.1063 0.1567 0.2622 0.1526 0.1001 0.0759 0.0689 0.1003 0.0254 0.1116 0.0269
Nuclear Periphery 0.0062 0.001 0.0058 0.0093 0.0302 0.0034 0.0518 0.0012 0.0022 0.0006 0.0013 0.0026 0.0035 0.0013 0.0011 0.0009 0.0015 0.0075
Nucleolus 0.0017 0.0007 0.0012 0.0015 0.0007 0.0007 0.0022 0.0009 0.0014 0.0007 0.0006 0.0003 0.0011 0.0007 0.0004 0.0009 0.0013 0.0006
Nucleus 0.0029 0.0014 0.003 0.0054 0.0105 0.0036 0.007 0.0014 0.0031 0.0013 0.0011 0.001 0.0012 0.0013 0.0008 0.0019 0.0017 0.0023
Peroxisomes 0.0392 0.0192 0.0363 0.0276 0.0462 0.0459 0.0455 0.0329 0.0292 0.0417 0.0894 0.0443 0.021 0.0251 0.0189 0.0112 0.0185 0.0428
Punctate Nuclear 0.0072 0.0008 0.0023 0.003 0.0038 0.0015 0.0163 0.0014 0.0028 0.0005 0.0008 0.0011 0.0008 0.0014 0.0007 0.0008 0.0024 0.0023
Vacuole 0.0124 0.0072 0.0099 0.015 0.0178 0.0074 0.011 0.0214 0.0082 0.0097 0.011 0.0072 0.0142 0.0088 0.0068 0.0384 0.0134 0.0078
Vacuole Periphery 0.005 0.0026 0.0064 0.0049 0.0094 0.0039 0.0046 0.0033 0.0029 0.0029 0.0058 0.003 0.0045 0.0026 0.0025 0.0073 0.0064 0.0055

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.228 3.5354 5.2468 6.5732 6.8369 2.6351 8.9366 10.0527 4.6177 6.7283
Translational Efficiency 0.3771 1.1775 0.5459 0.4807 0.4217 1.4596 0.7085 0.5734 0.7062 0.6569

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1635 1070 1662 435 1206 1770 487 1531 2841 2840 2149 1966

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 658.02 656.52 762.13 877.29 620.34 786.15 882.36 816.43 642.02 737.31 789.38 829.90
Standard Deviation 93.13 109.01 106.15 120.90 75.83 95.24 100.66 117.03 88.20 118.64 116.38 120.58
Intensity Change Log 2 — -0.003292 0.211906 0.414922 — 0.341745 0.508308 0.396270 — 0.174447 0.363346 0.405901

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000729 0.002359 0.000334 0.006422 0.000833 0.001389 0.001201 0.001565 0.000773 0.001754 0.000531 0.002640
Bud Neck 0.056343 0.048112 0.041957 0.016634 0.042505 0.047874 0.021193 0.039172 0.050469 0.047964 0.037252 0.034185
Bud Site 0.021352 0.059948 0.011458 0.039387 0.016590 0.026794 0.013139 0.037553 0.019331 0.039285 0.011839 0.037959
Cell Periphery 0.000261 0.000248 0.001885 0.000339 0.000220 0.000194 0.000305 0.000184 0.000244 0.000214 0.001527 0.000218
Cytoplasm 0.117584* 0.103425* 0.236164* 0.240556* 0.140256* 0.101272* 0.211840* 0.270219* 0.127208* 0.102083* 0.230652* 0.263656*
Cytoplasmic Foci 0.327049* 0.271224* 0.125372 0.148651 0.337270* 0.305303* 0.134124 0.143296 0.331388* 0.292463* 0.127355 0.144481
Eisosomes 0.000299 0.000166 0.001126 0.000185 0.000269 0.000199 0.000231 0.000134 0.000286 0.000187 0.000923 0.000146
Endoplasmic Reticulum 0.001497 0.000861 0.003734 0.006267 0.002067 0.000519 0.007772 0.002257 0.001739 0.000648 0.004649 0.003144
Endosome 0.108148 0.124155 0.049421 0.050954 0.070890 0.081629 0.047706 0.046753 0.092332 0.097651 0.049032 0.047682
Golgi 0.049663 0.056124 0.005393 0.005510 0.034533 0.061478 0.005433 0.008512 0.043240 0.059461 0.005402 0.007848
Lipid Particles 0.025250 0.009471 0.033573 0.008199 0.031388 0.015448 0.024674 0.009653 0.027856 0.013196 0.031556 0.009331
Mitochondria 0.115664* 0.091463 0.024566 0.003498 0.147661* 0.136656* 0.009891 0.021048 0.129247* 0.119629* 0.021241 0.017165
Mitotic Spindle 0.007289 0.027257 0.030531 0.029111 0.005646 0.019524 0.021596 0.044825 0.006591 0.022438 0.028507 0.041348
None 0.001921 0.006673 0.013577 0.004050 0.001966 0.001863 0.008209 0.004646 0.001940 0.003675 0.012361 0.004514
Nuclear Periphery 0.000476 0.000253 0.004088 0.002840 0.001104 0.000243 0.004889 0.001350 0.000743 0.000247 0.004269 0.001679
Nuclear Periphery Foci 0.000455 0.000648 0.001748 0.005520 0.000711 0.000290 0.006540 0.001098 0.000563 0.000424 0.002834 0.002076
Nucleolus 0.002879 0.004355 0.014537 0.004310 0.002790 0.002469 0.011689 0.006455 0.002841 0.003180 0.013892 0.005980
Nucleus 0.029837 0.023556 0.251477* 0.110838 0.045782 0.022642 0.295931* 0.133960* 0.036605 0.022986 0.261551* 0.128844
Peroxisomes 0.063263 0.109766* 0.017942 0.073445 0.064378 0.124174* 0.030153 0.056673 0.063736 0.118746* 0.020709 0.060384
Vacuole 0.061719 0.048843 0.123740 0.238466* 0.045535 0.044145 0.138764 0.164005 0.054849 0.045915 0.127145 0.180480
Vacuole Periphery 0.008323 0.011092 0.007376 0.004818 0.007608 0.005894 0.004719 0.006643 0.008019 0.007853 0.006774 0.006239

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.82 3.46 -2.59 -1.27 -2.84 -1.63 -2.22 -3.61 -0.21 -0.91 -2.47 2.29 -3.91 -0.75 -4.55
Bud Neck 2.05 3.93 13.56 9.90 10.26 -1.43 6.28 2.16 3.95 -4.84 0.97 4.85 7.75 6.81 2.68
Bud Site -9.50 5.25 -3.28 4.58 -5.75 -5.06 1.49 -6.57 -2.47 -7.21 -9.88 5.26 -6.63 2.06 -10.01
Cell Periphery 0.29 -4.70 0.14 -0.06 4.67 1.22 -2.70 3.32 2.01 5.74 1.15 -4.81 2.45 1.64 5.08
Cytoplasm 1.95 -15.74 -8.04 -8.81 0.93 5.62 -5.87 -14.82 -21.45 -4.93 5.24 -16.17 -19.30 -23.26 -4.20
Cytoplasmic Foci 6.38 30.53 22.83 15.30 0.42 3.67 22.55 27.23 26.37 0.01 6.44 38.91 36.93 30.04 -0.58
Eisosomes 4.99 -3.37 4.52 -0.10 3.96 4.58 2.16 10.13 7.18 6.25 6.52 -3.35 10.57 4.82 4.24
Endoplasmic Reticulum 2.57 -6.36 -6.36 -7.57 -2.70 6.11 -6.86 -0.42 -6.56 6.67 6.34 -8.88 -4.32 -9.38 4.56
Endosome -2.38 12.92 13.47 13.73 2.85 -2.39 5.18 8.11 11.87 1.53 -1.36 13.17 16.58 18.07 3.55
Golgi -1.63 17.39 18.20 17.17 1.87 -7.95 12.86 12.48 20.92 -1.35 -6.21 21.27 21.27 26.60 -0.56
Lipid Particles 7.84 -2.50 9.00 1.46 10.94 6.82 2.55 10.44 4.90 5.69 9.37 -0.98 13.03 4.69 11.89
Mitochondria 3.35 17.59 22.74 15.91 11.68 1.40 19.67 19.11 22.70 -2.62 1.85 25.45 27.61 26.59 4.10
Mitotic Spindle -6.69 -7.80 -4.22 0.31 1.05 -7.68 -3.86 -10.55 -6.26 -4.16 -9.83 -8.92 -11.14 -5.26 -2.71
None -2.95 -6.84 -0.83 1.93 5.07 0.12 -2.65 -3.61 -3.53 1.54 -2.58 -7.38 -3.58 -0.35 5.47
Nuclear Periphery 2.66 -8.78 -4.44 -4.92 2.90 3.23 -3.47 -1.84 -12.94 3.05 4.04 -8.76 -6.18 -11.86 6.19
Nuclear Periphery Foci -1.40 -7.16 -6.74 -6.53 -4.94 2.69 -5.60 -3.68 -5.79 4.54 1.58 -8.24 -7.60 -8.36 0.75
Nucleolus -1.94 -8.55 -0.57 0.64 6.16 0.22 -5.44 -4.37 -4.51 2.15 -1.01 -9.93 -4.25 -3.52 5.70
Nucleus 1.70 -32.12 -9.83 -10.30 12.43 6.53 -18.24 -15.47 -21.68 11.02 5.78 -36.32 -20.50 -23.97 17.54
Peroxisomes -7.62 14.41 2.11 7.47 -5.02 -10.93 7.34 3.64 14.36 -3.86 -13.70 17.12 4.47 16.18 -8.76
Vacuole 3.08 -13.22 -20.14 -20.98 -14.41 0.42 -12.69 -25.56 -26.04 -4.60 3.38 -18.72 -30.79 -32.47 -14.11
Vacuole Periphery -2.28 1.46 4.12 5.02 2.87 2.68 4.62 3.33 1.33 -0.62 0.35 2.41 5.12 4.36 2.29
Standard name
Human Ortholog
Description Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase; may comprise a type II mitochondrial fatty acid synthase along with Mct1p; human homolog CBR4 complements yeast null mutant
Localization
Cell Percentages mitochondrion (49%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Oar1

Oar1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Oar1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available