ORF | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 7 | 3 | 6 | 6 | 5 | – | 15 | 1 | 8 | 9 | 7 | 7 | 1 | 2 | 5 | 11 | 6 | 10 | 9 | 2 | 5 | 6 | 10 |
Bud | 13 | 1 | 20 | 20 | 11 | 25 | – | 65 | 20 | 31 | 22 | 35 | 2 | 1 | 2 | 3 | 9 | 0 | 1 | 0 | 0 | 1 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 1 | 0 | 0 | 0 | – | 8 | 3 | 2 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 0 | 0 | 1 | 0 | – | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 92 | 54 | 49 | 29 | 30 | 38 | – | 32 | 16 | 16 | 20 | 28 | 27 | 10 | 18 | 51 | 39 | 26 | 8 | 1 | 1 | 0 | 0 | 3 |
Endoplasmic Reticulum | 6 | 8 | 1 | 6 | 5 | 6 | – | 2 | 2 | 1 | 2 | 5 | 12 | 3 | 3 | 8 | 5 | 7 | 3 | 1 | 2 | 2 | 3 | 4 |
Endosome | 251 | 50 | 47 | 22 | 99 | 74 | – | 113 | 20 | 13 | 12 | 24 | 97 | 33 | 64 | 103 | 110 | 58 | 292 | 104 | 126 | 82 | 51 | 72 |
Golgi | 38 | 26 | 2 | 8 | 11 | 8 | – | 9 | 0 | 8 | 8 | 12 | 53 | 42 | 48 | 15 | 11 | 4 | 248 | 84 | 58 | 115 | 98 | 148 |
Mitochondria | 331 | 71 | 188 | 126 | 71 | 260 | – | 344 | 194 | 265 | 215 | 269 | 86 | 26 | 38 | 19 | 28 | 13 | 74 | 40 | 43 | 27 | 20 | 21 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 2 | 0 | 0 | – | 0 | 1 | 1 | 1 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 1 | 1 | 2 | 1 | 8 | – | 24 | 9 | 13 | 7 | 11 | 2 | 1 | 1 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 27 | 0 | 14 | 10 | 11 | 23 | – | 63 | 21 | 17 | 11 | 42 | 19 | 2 | 7 | 7 | 2 | 1 | 20 | 3 | 17 | 15 | 9 | 5 |
SpindlePole | 32 | 6 | 29 | 6 | 15 | 17 | – | 46 | 18 | 19 | 11 | 27 | 10 | 7 | 13 | 29 | 25 | 21 | 5 | 2 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | 139 | 42 | 24 | 3 | 9 | 12 | – | 15 | 5 | 15 | 11 | 16 | 8 | 2 | 9 | 149 | 92 | 80 | 122 | 44 | 55 | 13 | 11 | 15 |
Unique Cell Count | 700 | 215 | 286 | 190 | 226 | 372 | 534 | 251 | 331 | 262 | 382 | 239 | 101 | 161 | 279 | 247 | 157 | 792 | 297 | 314 | 268 | 209 | 285 | |
Labelled Cell Count | 943 | 268 | 379 | 240 | 270 | 476 | 738 | 311 | 412 | 336 | 490 | 325 | 130 | 205 | 393 | 339 | 217 | 792 | 297 | 314 | 268 | 209 | 285 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 6.9 | 4.8 | 5.8 | 6.2 | 5.5 | – | 5.7 | 5.8 | 5.9 | 6.3 | 6.2 | 6.6 | 6.9 | 6.7 | 9.3 | 9.0 | 8.4 | 7.2 | 7.6 | 7.4 |
Std Deviation (1e-4) | 1.0 | 1.2 | 1.1 | 1.3 | 1.5 | 1.2 | – | 1.1 | 1.5 | 1.3 | 1.3 | 1.4 | 1.1 | 1.1 | 1.1 | 1.7 | 1.7 | 1.6 | 1.5 | 1.5 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.26 | 0.35 | 0.18 | – | 0.23 | 0.25 | 0.29 | 0.38 | 0.36 | 0.45 | 0.51 | 0.47 | 0.95 | 0.9 | 0.79 | 0.56 | 0.65 | 0.6 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 1.6 | 0 | 1.3 | 1.9 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 |
Bud | 1.4 | -1.0 | -0.1 | – | 2.3 | 0.4 | 1.1 | 0.6 | 1.0 | -3.5 | -2.3 | -2.7 | -3.6 | -1.7 | -3.4 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.5 | -1.2 | -2.6 | – | -5.1 | -3.8 | -5.0 | -3.3 | -3.9 | -1.9 | -1.7 | -1.7 | 0.4 | -0.4 | -0.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.5 | 6.8 | 1.1 | – | 1.6 | -3.0 | -5.2 | -4.5 | -4.2 | 6.2 | 3.5 | 5.5 | 5.5 | 7.1 | 4.9 |
Golgi | 0 | 3.0 | 0 | – | 0 | 0 | 0 | 0 | 2.2 | 8.0 | 11.1 | 9.4 | 3.3 | 2.8 | 0 |
Mitochondria | 0.1 | -7.7 | 1.1 | – | -0.4 | 2.9 | 4.0 | 4.3 | 1.3 | -6.8 | -6.9 | -8.6 | -14.5 | -12.8 | -11.6 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 3.3 | 0 | 3.0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.2 | 0 | 0.7 | – | 3.2 | 1.6 | 0.1 | -0.4 | 2.8 | 1.4 | 0 | -0.3 | -1.5 | -2.8 | -2.4 |
SpindlePole | -2.9 | -1.4 | -2.8 | – | -0.7 | -1.2 | -2.0 | -2.7 | -1.4 | -2.6 | -1.0 | -0.7 | 0.1 | 0 | 1.0 |
Vacuole | -3.1 | -2.0 | -2.9 | – | -3.6 | -3.3 | -2.0 | -2.0 | -2.3 | -2.4 | -2.2 | -1.1 | 11.6 | 8.1 | 10.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.2228 | 6.6264 | 6.286 | 5.0717 | 4.8048 | 5.9532 | 3.832 | 4.975 | 4.7096 | 4.3301 | 4.07 | 4.6613 | 6.6933 | 7.1031 | 7.058 | 6.5132 | 6.2014 | 6.8721 |
Actin | 0.0633 | 0.0242 | 0.0148 | 0.0009 | 0.0082 | 0.0019 | 0.0215 | 0.0085 | 0.037 | 0.0029 | 0.0423 | 0.012 | 0.0183 | 0.0117 | 0.0107 | 0.0144 | 0.0026 | 0.0186 |
Bud | 0.0006 | 0.0002 | 0.002 | 0 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0003 | 0 | 0.0007 |
Bud Neck | 0.0013 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0054 | 0.0003 | 0.0003 | 0.0004 | 0.0009 | 0.0002 | 0.0003 | 0.0005 | 0.0003 | 0.0003 |
Bud Periphery | 0.0007 | 0.0002 | 0.0026 | 0 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0003 | 0 | 0.0004 |
Bud Site | 0.0021 | 0.0005 | 0.0063 | 0.0002 | 0.0016 | 0.0001 | 0.0005 | 0.0002 | 0.0051 | 0.0005 | 0.0016 | 0.0002 | 0.002 | 0.0005 | 0.0006 | 0.002 | 0.0001 | 0.0003 |
Cell Periphery | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 |
Cytoplasm | 0.0025 | 0.0012 | 0.0063 | 0.0006 | 0.0006 | 0.0006 | 0.0052 | 0.0014 | 0.001 | 0.0017 | 0.0007 | 0.0013 | 0.0013 | 0.0021 | 0.002 | 0.0008 | 0.001 | 0.0011 |
Cytoplasmic Foci | 0.0934 | 0.109 | 0.0803 | 0.181 | 0.076 | 0.0584 | 0.142 | 0.1866 | 0.1277 | 0.1268 | 0.1053 | 0.1257 | 0.1284 | 0.1682 | 0.1277 | 0.1262 | 0.1281 | 0.115 |
Eisosomes | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0032 | 0.0004 | 0.0022 | 0.0001 | 0.0005 | 0.0006 | 0.0023 | 0.0002 | 0.0002 | 0.0007 | 0.0002 | 0.0008 | 0.0008 | 0.0002 | 0.0005 | 0.0002 | 0.0023 | 0.0003 |
Endosome | 0.1554 | 0.2013 | 0.1519 | 0.259 | 0.2701 | 0.1416 | 0.2018 | 0.1843 | 0.1704 | 0.3553 | 0.3021 | 0.157 | 0.2288 | 0.2179 | 0.1932 | 0.2117 | 0.24 | 0.1699 |
Golgi | 0.5502 | 0.6136 | 0.6826 | 0.4265 | 0.4899 | 0.7641 | 0.5082 | 0.5474 | 0.601 | 0.4343 | 0.5216 | 0.6086 | 0.5074 | 0.5579 | 0.6017 | 0.6005 | 0.5236 | 0.6247 |
Lipid Particles | 0.0681 | 0.0191 | 0.0103 | 0.0273 | 0.0375 | 0.018 | 0.0325 | 0.0261 | 0.0082 | 0.0117 | 0.015 | 0.0185 | 0.0412 | 0.0181 | 0.0201 | 0.0154 | 0.045 | 0.0166 |
Mitochondria | 0.0191 | 0.0091 | 0.016 | 0.0042 | 0.007 | 0.0043 | 0.0094 | 0.0042 | 0.0038 | 0.0379 | 0.0034 | 0.0074 | 0.0168 | 0.0016 | 0.0077 | 0.0046 | 0.003 | 0.0072 |
None | 0.0011 | 0.0001 | 0.0039 | 0.0001 | 0.0007 | 0 | 0.0033 | 0 | 0.0001 | 0.0004 | 0 | 0.0004 | 0.0003 | 0.0003 | 0.0013 | 0 | 0.0006 | 0.0001 |
Nuclear Periphery | 0.0042 | 0.0002 | 0.0018 | 0 | 0.002 | 0.0001 | 0.006 | 0.0001 | 0.0001 | 0.0024 | 0.0001 | 0.0016 | 0.0016 | 0.0001 | 0.0008 | 0.0001 | 0.0314 | 0.0001 |
Nucleolus | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0025 | 0 | 0.0002 | 0.0025 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 |
Nucleus | 0.0014 | 0 | 0.0006 | 0 | 0.0004 | 0 | 0.0024 | 0 | 0.0001 | 0.0036 | 0 | 0.0002 | 0.0003 | 0 | 0.0003 | 0 | 0.0053 | 0 |
Peroxisomes | 0.0263 | 0.0157 | 0.0142 | 0.0972 | 0.0208 | 0.0072 | 0.0509 | 0.0394 | 0.0375 | 0.0076 | 0.0039 | 0.0624 | 0.0474 | 0.0184 | 0.0287 | 0.0211 | 0.0092 | 0.0425 |
Punctate Nuclear | 0.0025 | 0.0002 | 0.0007 | 0.0012 | 0.0745 | 0.0001 | 0.0065 | 0.0002 | 0.0004 | 0.0005 | 0.0001 | 0.0018 | 0.001 | 0.0004 | 0.0014 | 0.0001 | 0.0014 | 0.0002 |
Vacuole | 0.0019 | 0.0023 | 0.0011 | 0.0005 | 0.0024 | 0.0007 | 0.0021 | 0.0005 | 0.0005 | 0.0042 | 0.001 | 0.0006 | 0.0011 | 0.0013 | 0.0009 | 0.0007 | 0.002 | 0.0006 |
Vacuole Periphery | 0.0019 | 0.0023 | 0.0012 | 0.001 | 0.0062 | 0.002 | 0.0018 | 0.0006 | 0.0006 | 0.0056 | 0.0014 | 0.0009 | 0.0013 | 0.0009 | 0.0014 | 0.0009 | 0.0036 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 33.9341 | 25.4422 | 24.6882 | 47.5003 | 42.5777 | 45.9497 | 30.177 | 27.3792 | 37.6464 | 51.7952 |
Translational Efficiency | 3.0591 | 3.1285 | 3.0369 | 1.98 | 2.2755 | 2.2887 | 2.6896 | 3.2057 | 2.4386 | 1.9964 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1193 | 1715 | 1516 | 185 | 2141 | 1556 | 2126 | 706 | 3334 | 3271 | 3642 | 891 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 715.46 | 883.40 | 1016.63 | 942.12 | 827.65 | 872.85 | 882.89 | 848.17 | 787.51 | 878.38 | 938.56 | 867.68 |
Standard Deviation | 84.32 | 127.97 | 132.33 | 115.52 | 421.18 | 122.35 | 117.02 | 126.85 | 345.48 | 125.44 | 140.10 | 130.28 |
Intensity Change Log 2 | — | 0.304196 | 0.506852 | 0.397040 | — | 0.076713 | 0.093213 | 0.035333 | — | 0.186657 | 0.299794 | 0.214352 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001500 | 0.005832 | 0.010260 | 0.034189 | 0.005677 | 0.002575 | 0.021137 | 0.000670 | 0.004183 | 0.004283 | 0.016609 | 0.007630 |
Bud Neck | 0.000136 | 0.000155 | 0.000345 | 0.006668 | 0.000046 | 0.000143 | 0.001295 | 0.011890 | 0.000078 | 0.000149 | 0.000899 | 0.010806 |
Bud Site | 0.000245 | 0.001200 | 0.003454 | 0.040712 | 0.000205 | 0.000398 | 0.008335 | 0.032111 | 0.000220 | 0.000818 | 0.006303 | 0.033897 |
Cell Periphery | 0.000023 | 0.000042 | 0.000035 | 0.000113 | 0.000025 | 0.000020 | 0.000043 | 0.000244 | 0.000024 | 0.000032 | 0.000040 | 0.000217 |
Cytoplasm | 0.000866 | 0.001547 | 0.001405 | 0.091270 | 0.001411 | 0.000506 | 0.008968 | 0.087294 | 0.001216 | 0.001052 | 0.005820 | 0.088119 |
Cytoplasmic Foci | 0.299486* | 0.292630* | 0.442595* | 0.277188* | 0.283204* | 0.274948* | 0.327127* | 0.173296 | 0.289030* | 0.284219* | 0.375191* | 0.194867* |
Eisosomes | 0.000058 | 0.000049 | 0.000041 | 0.000106 | 0.000051 | 0.000033 | 0.000078 | 0.000048 | 0.000053 | 0.000042 | 0.000063 | 0.000060 |
Endoplasmic Reticulum | 0.000080 | 0.000113 | 0.000805 | 0.001848 | 0.000092 | 0.000064 | 0.000622 | 0.001760 | 0.000088 | 0.000090 | 0.000698 | 0.001779 |
Endosome | 0.145958 | 0.247643* | 0.201191* | 0.105525 | 0.247876* | 0.258293* | 0.308928* | 0.249367* | 0.211407* | 0.252709* | 0.264082* | 0.219500* |
Golgi | 0.455538* | 0.407743* | 0.270158* | 0.021064 | 0.416616* | 0.428888* | 0.195362* | 0.013843 | 0.430544* | 0.417802* | 0.226496* | 0.015342 |
Lipid Particles | 0.031263 | 0.009617 | 0.012314 | 0.016078 | 0.019490 | 0.009792 | 0.025696 | 0.020795 | 0.023703 | 0.009700 | 0.020126 | 0.019815 |
Mitochondria | 0.009114 | 0.006968 | 0.000719 | 0.002859 | 0.003926 | 0.006043 | 0.005755 | 0.006138 | 0.005782 | 0.006528 | 0.003659 | 0.005457 |
Mitotic Spindle | 0.001582 | 0.001995 | 0.031880 | 0.070261 | 0.000319 | 0.000819 | 0.040260 | 0.080715 | 0.000771 | 0.001435 | 0.036772 | 0.078544 |
None | 0.000068 | 0.000016 | 0.000007 | 0.001087 | 0.000240 | 0.000008 | 0.000024 | 0.001734 | 0.000178 | 0.000012 | 0.000017 | 0.001599 |
Nuclear Periphery | 0.000010 | 0.000015 | 0.000102 | 0.000273 | 0.000019 | 0.000014 | 0.000325 | 0.001640 | 0.000016 | 0.000014 | 0.000232 | 0.001356 |
Nuclear Periphery Foci | 0.000502 | 0.000153 | 0.001864 | 0.002945 | 0.000094 | 0.000055 | 0.002213 | 0.005811 | 0.000240 | 0.000107 | 0.002068 | 0.005216 |
Nucleolus | 0.000121 | 0.000037 | 0.000009 | 0.000213 | 0.000007 | 0.000125 | 0.000143 | 0.004056 | 0.000047 | 0.000079 | 0.000088 | 0.003259 |
Nucleus | 0.000033 | 0.000045 | 0.000026 | 0.006881 | 0.001214 | 0.000506 | 0.000599 | 0.016490 | 0.000791 | 0.000264 | 0.000361 | 0.014495 |
Peroxisomes | 0.052473 | 0.019478 | 0.010227 | 0.122236* | 0.017961 | 0.012128 | 0.025111 | 0.022748 | 0.030310 | 0.015982 | 0.018915 | 0.043405 |
Vacuole | 0.000489 | 0.003096 | 0.010792 | 0.191941 | 0.000699 | 0.002467 | 0.025368 | 0.259739* | 0.000624 | 0.002797 | 0.019301 | 0.245662* |
Vacuole Periphery | 0.000455 | 0.001624 | 0.001771 | 0.006542 | 0.000829 | 0.002175 | 0.002610 | 0.009611 | 0.000695 | 0.001886 | 0.002261 | 0.008974 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.69 | -6.61 | -2.87 | -2.17 | -1.42 | 2.95 | -6.87 | 5.30 | 4.03 | 10.03 | -0.14 | -8.60 | -0.26 | -0.19 | 6.48 |
Bud Neck | -0.58 | -4.61 | -4.78 | -4.77 | -4.64 | -2.07 | -11.15 | -8.21 | -8.14 | -7.08 | -2.44 | -11.94 | -9.24 | -9.17 | -8.35 |
Bud Site | -2.81 | -8.73 | -5.39 | -5.24 | -4.87 | -1.94 | -10.62 | -7.74 | -7.69 | -5.12 | -3.22 | -12.83 | -9.34 | -9.13 | -7.14 |
Cell Periphery | -2.27 | -4.03 | -6.61 | -4.51 | -5.67 | 0.82 | -2.73 | -1.39 | -1.45 | -1.21 | -1.12 | -3.51 | -1.61 | -1.53 | -1.42 |
Cytoplasm | -1.20 | -2.20 | -8.78 | -8.73 | -8.74 | 1.58 | -8.29 | -15.40 | -15.58 | -13.76 | 0.32 | -8.29 | -17.75 | -17.79 | -16.67 |
Cytoplasmic Foci | 1.05 | -20.23 | 7.22 | 6.84 | 16.38 | 1.47 | -6.85 | 18.07 | 16.14 | 22.33* | 1.14 | -18.42 | 19.82 | 18.87 | 31.32* |
Eisosomes | 1.09 | 2.32 | -3.20 | -4.31 | -5.30 | 3.50 | -3.89 | 0.50 | -4.14 | 4.90 | 2.66 | -1.94 | -0.99 | -4.23 | 1.13 |
Endoplasmic Reticulum | -1.98 | -8.32 | -4.83 | -4.75 | -3.01 | 0.92 | -8.14 | -8.22 | -8.44 | -4.75 | -0.15 | -11.49 | -9.56 | -9.59 | -5.50 |
Endosome | -17.08 | -10.03 | 9.23 | 20.13 | 15.57 | -1.73 | -8.91 | 6.15 | 6.94 | 11.34 | -9.49 | -11.00 | 6.14 | 11.24 | 12.11 |
Golgi | 5.77 | 22.56 | 63.26* | 69.70* | 45.59* | -1.81 | 37.61* | 90.73* | 77.43* | 40.62* | 2.47 | 42.30 | 110.47* | 106.53* | 60.79* |
Lipid Particles | 7.32 | 6.39 | 5.80 | -0.74 | 0.51 | 4.30 | -2.70 | 0.92 | -2.84 | 3.41 | 7.98 | 1.67 | 3.45 | -3.12 | 2.34 |
Mitochondria | 1.27 | 5.96 | 4.66 | 4.29 | -0.93 | -1.97 | -1.86 | -0.25 | 1.07 | 0.91 | -0.82 | 2.74 | 1.62 | 2.19 | -0.06 |
Mitotic Spindle | -0.75 | -10.35 | -4.28 | -4.23 | -1.47 | -1.71 | -14.91 | -10.07 | -10.00 | -4.04 | -2.05 | -18.17 | -10.89 | -10.78 | -4.71 |
None | 1.23 | 1.46 | -2.21 | -2.31 | -2.32 | 1.38 | 1.24 | -2.03 | -2.48 | -2.44 | 1.52 | 1.44 | -2.56 | -2.95 | -2.93 |
Nuclear Periphery | -1.08 | -7.06 | -7.10 | -7.03 | -6.03 | 0.59 | -5.75 | -7.38 | -7.41 | -5.57 | 0.19 | -6.64 | -8.04 | -8.05 | -6.53 |
Nuclear Periphery Foci | 0.84 | -2.03 | -3.66 | -4.51 | -2.23 | 0.60 | -6.19 | -7.82 | -7.90 | -4.04 | 0.85 | -5.69 | -8.31 | -8.85 | -4.73 |
Nucleolus | 0.96 | 1.32 | -2.33 | -3.77 | -4.11 | -1.06 | -2.50 | -4.75 | -4.54 | -4.46 | -0.47 | -1.29 | -4.82 | -4.75 | -4.69 |
Nucleus | -0.53 | 0.14 | -6.93 | -6.93 | -6.93 | 0.91 | -0.16 | -7.87 | -8.33 | -8.05 | 1.15 | 0.01 | -9.60 | -10.06 | -9.79 |
Peroxisomes | 8.62 | 11.80 | -1.62 | -4.56 | -5.40 | 3.19 | -3.36 | -0.47 | -3.11 | 2.46 | 7.60 | 5.91 | 0.11 | -4.55 | -3.53 |
Vacuole | -4.36 | -10.65 | -15.37 | -15.26 | -14.92 | -4.62 | -15.05 | -28.50 | -28.34 | -25.36 | -5.92 | -17.51 | -32.36 | -32.13 | -29.94 |
Vacuole Periphery | -5.70 | -4.17 | -1.06 | -0.62 | -0.56 | -3.15 | -5.28 | -4.32 | -3.23 | -3.00 | -4.81 | -6.43 | -4.39 | -3.36 | -3.07 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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