Standard name
Human Ortholog
Description Molecular chaperone of the endoplasmic reticulum lumen; involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 0.61 0.92 0.94 0.85 0.86 0.83 0.84 0.84 – 0.81 0.85 0.93 0.99 0.99 0.35 0.4 0.48 0.1 0.1 0.2 0.22 0.16 0.22
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.08 0.05 0 0 0 0 0 0
Endosome 0.06 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0.05 0.09 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.09 0 0 0 0 0.05 0 0.16 – 0.21 0.12 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0.06 0.05 0 – 0 0 0 0 0 0.12 0.09 0.11 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.37 0.37 0.09 0.09 0.24 0.19 0.19 0.21 0.22 – 0.15 0.14 0.05 0 0 0.64 0.62 0.57 0.76 0.75 0.68 0.68 0.74 0.62
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 2 0 0 0 1 2
Bud 0 0 2 0 0 0 1 3 2 – 1 1 2 1 1 0 0 0 1 1 1 2 4 7
Bud Neck 0 0 8 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 2 1 1 3
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 160 114 300 201 311 269 410 364 332 – 261 276 205 241 290 45 35 45 32 21 71 35 52 55
Endoplasmic Reticulum 3 7 1 0 1 0 2 1 0 – 0 0 0 0 0 2 7 5 3 0 1 5 4 6
Endosome 15 6 2 0 2 3 0 1 0 – 0 0 1 1 1 6 3 1 16 19 8 0 3 3
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 2 0 2 1 0 0 0 2
Mitochondria 11 16 0 1 1 10 23 16 62 – 69 40 2 0 0 1 2 1 6 5 16 6 12 8
Nucleus 5 3 3 3 5 5 32 20 6 – 11 6 0 0 1 15 8 10 1 0 1 0 0 0
Nuclear Periphery 0 2 0 0 0 0 0 2 0 – 3 1 1 0 1 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 0 1 0 0 0 – 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 1 1 2 0 0 1
Vac/Vac Membrane 99 69 28 19 89 61 93 91 86 – 48 47 12 3 3 82 55 53 235 164 240 111 240 153
Unique Cell Count 265 186 326 214 365 313 494 432 397 321 325 221 243 294 128 88 93 310 221 356 164 325 248
Labelled Cell Count 295 217 345 224 409 350 562 498 488 395 372 224 246 297 151 112 115 310 221 356 164 325 248


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.5 5.0 4.6 5.5 4.7 4.5 4.8 4.2 – 3.9 4.3 4.2 4.1 4.3 7.9 7.9 7.5 4.3 4.3 4.8
Std Deviation (1e-4) 0.7 0.9 1.3 1.4 1.5 1.7 1.1 1.4 1.3 – 1.5 1.1 1.3 1.5 1.8 2.2 2.2 2.6 1.6 1.6 1.8
Intensity Change (Log2) – – – -0.11 0.13 -0.09 -0.16 -0.05 -0.27 – -0.36 -0.22 -0.24 -0.27 -0.23 0.66 0.66 0.58 -0.22 -0.24 -0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 12.4 11.2 10.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5191 2.394 2.1826 1.3739 1.9102 1.8068 -0.3469 0.2224 -0.0559 -0.1171 -0.4109 -0.1548 -0.0808 0.8652 0.514 0.5162 0.2699 0.9296
Actin 0.0204 0.0026 0.011 0.0051 0.0206 0 0.0103 0.0001 0.0031 0.0205 0.0027 0.0014 0.0057 0.0011 0.0013 0.0012 0.0112 0.0013
Bud 0.0008 0.0008 0.0005 0.0022 0.0013 0.0004 0.0014 0.0076 0.0047 0.002 0.0006 0.002 0.0159 0.0397 0.0378 0.0251 0.0221 0.0181
Bud Neck 0.0175 0.0016 0.0008 0.002 0.003 0.0075 0.0045 0.0021 0.0016 0.0025 0.0011 0.0031 0.0018 0.004 0.0035 0.0039 0.0067 0.0147
Bud Periphery 0.0019 0.0014 0.0014 0.0085 0.0025 0.0013 0.0036 0.0066 0.0059 0.0021 0.0014 0.0025 0.0141 0.0244 0.0247 0.0219 0.0271 0.0175
Bud Site 0.009 0.0045 0.0021 0.003 0.0033 0.001 0.0057 0.01 0.0129 0.0057 0.0009 0.001 0.0222 0.1061 0.0325 0.011 0.0076 0.0045
Cell Periphery 0.0021 0.0028 0.0014 0.0016 0.0009 0.0018 0.0013 0.0017 0.0009 0.0007 0.0003 0.0009 0.0024 0.0033 0.0022 0.0032 0.002 0.002
Cytoplasm 0.1134 0.1341 0.0795 0.0516 0.0589 0.1099 0.1053 0.1174 0.1014 0.0662 0.0602 0.1026 0.0828 0.1165 0.075 0.0774 0.06 0.1581
Cytoplasmic Foci 0.0595 0.0315 0.0465 0.0503 0.0809 0.0376 0.0706 0.0694 0.0815 0.0618 0.0469 0.0612 0.1514 0.083 0.1174 0.1454 0.2077 0.1331
Eisosomes 0.0006 0.0003 0.0003 0.0005 0.0004 0.0001 0.0004 0 0.0001 0.0001 0.0001 0 0.0003 0.0001 0.0002 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0122 0.0035 0.0062 0.0076 0.0029 0.005 0.0069 0.0054 0.0073 0.0033 0.0038 0.0037 0.0078 0.0059 0.0027 0.0028 0.0022 0.0036
Endosome 0.1347 0.0614 0.1441 0.2153 0.1318 0.119 0.2789 0.2123 0.2841 0.3513 0.2801 0.2509 0.165 0.1082 0.136 0.1116 0.1408 0.089
Golgi 0.0072 0.0019 0.0089 0.0193 0.0114 0.0017 0.0219 0.004 0.0126 0.0218 0.0275 0.0051 0.0053 0.0028 0.0044 0.0082 0.0155 0.0025
Lipid Particles 0.034 0.01 0.0181 0.0136 0.0534 0.0126 0.0345 0.0134 0.0139 0.0216 0.0333 0.0083 0.0234 0.0081 0.0261 0.0065 0.0291 0.013
Mitochondria 0.0222 0.0133 0.0161 0.047 0.0061 0.0094 0.0381 0.0165 0.0248 0.0293 0.0536 0.0109 0.0168 0.0095 0.015 0.0396 0.0145 0.0042
None 0.3704 0.3906 0.4216 0.2082 0.1864 0.2505 0.1671 0.1339 0.1557 0.0489 0.0881 0.1125 0.295 0.2186 0.2966 0.2955 0.1768 0.2516
Nuclear Periphery 0.0108 0.0028 0.0065 0.0026 0.0035 0.0047 0.0128 0.0031 0.0054 0.0031 0.0038 0.0035 0.0057 0.0069 0.005 0.0037 0.0035 0.0074
Nucleolus 0.0165 0.0057 0.0046 0.0034 0.0043 0.0035 0.0055 0.0035 0.0044 0.0071 0.0014 0.0029 0.0091 0.0141 0.0164 0.0167 0.0211 0.0126
Nucleus 0.0089 0.0085 0.0069 0.0056 0.007 0.0094 0.0172 0.0082 0.0062 0.0056 0.004 0.0156 0.0121 0.0247 0.0267 0.0204 0.0152 0.0558
Peroxisomes 0.0143 0.0023 0.0125 0.0025 0.0464 0.0017 0.0066 0.0014 0.0135 0.0242 0.0667 0.0017 0.0068 0.0026 0.0054 0.0042 0.0244 0.0026
Punctate Nuclear 0.0051 0.0009 0.0014 0.001 0.0167 0.0006 0.0026 0.0021 0.0035 0.0014 0.0006 0.0043 0.0183 0.0155 0.0341 0.0381 0.0275 0.0454
Vacuole 0.1262 0.3011 0.1893 0.3044 0.3258 0.3843 0.1847 0.3468 0.2226 0.2977 0.2947 0.3707 0.1262 0.1908 0.1228 0.1444 0.1727 0.1501
Vacuole Periphery 0.0122 0.0184 0.0201 0.0446 0.0325 0.038 0.0202 0.0344 0.0338 0.0231 0.0283 0.0352 0.0119 0.014 0.0143 0.0191 0.0123 0.0128

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.0369 33.923 29.5982 34.9169 37.8469 41.261 36.4668 37.0143 33.5299 46.4245
Translational Efficiency 1.2606 1.258 1.0682 0.9886 1.1009 1.2347 0.8538 0.836 0.9476 0.9076

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1805 496 760 267 1865 2582 1761 89 3670 3078 2521 356

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 656.14 780.36 946.27 947.80 721.03 746.95 867.97 950.02 689.12 752.33 891.57 948.36
Standard Deviation 75.47 99.03 129.69 101.62 84.89 129.30 102.89 134.13 86.69 125.52 117.29 110.65
Intensity Change Log 2 — 0.250136 0.528248 0.530579 — 0.050952 0.267586 0.397899 — 0.149286 0.397659 0.462637

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000061 0.000216 0.000473 0.000376 0.000134 0.000224 0.000176 0.000301 0.000098 0.000223 0.000265 0.000357
Bud Neck 0.036778 0.025971 0.009107 0.010248 0.021525 0.043109 0.005482 0.003508 0.029027 0.040347 0.006575 0.008563
Bud Site 0.010552 0.009962 0.010997 0.011742 0.007154 0.024493 0.010684 0.113169* 0.008825 0.022151 0.010778 0.037099
Cell Periphery 0.000146 0.000091 0.000230 0.000291 0.000065 0.000146 0.000153 0.000298 0.000105 0.000137 0.000176 0.000293
Cytoplasm 0.244546* 0.281862* 0.294072* 0.237691* 0.327018* 0.255199* 0.443738* 0.393133* 0.286457* 0.259496* 0.398618* 0.276552*
Cytoplasmic Foci 0.089021 0.147174 0.025903 0.054920 0.100200 0.127421 0.016624 0.029301 0.094702 0.130604 0.019422 0.048515
Eisosomes 0.000074 0.000071 0.000077 0.000173 0.000053 0.000088 0.000044 0.000062 0.000064 0.000085 0.000054 0.000145
Endoplasmic Reticulum 0.001141 0.000583 0.010194 0.008637 0.000870 0.000915 0.002761 0.001628 0.001003 0.000862 0.005002 0.006885
Endosome 0.089280 0.079284 0.014349 0.007539 0.045045 0.087685 0.003890 0.004161 0.066801 0.086331 0.007043 0.006694
Golgi 0.001100 0.003938 0.000138 0.000094 0.001147 0.004511 0.000124 0.000109 0.001124 0.004419 0.000128 0.000098
Lipid Particles 0.005053 0.003171 0.005170 0.004479 0.003346 0.005080 0.001689 0.001808 0.004185 0.004772 0.002739 0.003812
Mitochondria 0.002540 0.004462 0.000457 0.000170 0.002081 0.008807 0.000926 0.000403 0.002307 0.008107 0.000784 0.000228
Mitotic Spindle 0.000079 0.006331 0.004080 0.000401 0.001710 0.004008 0.003850 0.062318 0.000908 0.004383 0.003919 0.015880
None 0.001099 0.001753 0.002216 0.001981 0.000519 0.001410 0.003048 0.013423 0.000804 0.001465 0.002797 0.004841
Nuclear Periphery 0.000329 0.000317 0.004022 0.002066 0.000339 0.000266 0.002217 0.001408 0.000334 0.000274 0.002761 0.001901
Nuclear Periphery Foci 0.000211 0.000471 0.010498 0.007259 0.000247 0.000209 0.001658 0.005409 0.000229 0.000251 0.004323 0.006797
Nucleolus 0.001251 0.000991 0.002002 0.000415 0.000949 0.001325 0.000454 0.000638 0.001098 0.001271 0.000921 0.000471
Nucleus 0.036532 0.043067 0.381070* 0.213025* 0.062350 0.032805 0.401748* 0.092412 0.049652 0.034459 0.395514* 0.182872*
Peroxisomes 0.003495 0.013107 0.000805 0.002182 0.002922 0.007661 0.000438 0.000230 0.003204 0.008539 0.000549 0.001694
Vacuole 0.471817* 0.373573* 0.223596* 0.436189* 0.419951* 0.387988* 0.100065 0.276103* 0.445460* 0.385665* 0.137306 0.396168*
Vacuole Periphery 0.004893 0.003607 0.000542 0.000122 0.002374 0.006651 0.000231 0.000180 0.003613 0.006161 0.000325 0.000137

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.82 -10.14 -9.96 -2.47 3.33 -1.14 -1.17 -2.75 -2.04 -2.92 -2.32 -5.86 -6.85 -3.71 -1.83
Bud Neck 3.51 10.40 9.29 4.71 0.89 -10.37 10.14 11.17 21.79 2.48 -6.05 15.53 10.34 14.97 0.01
Bud Site 0.52 -0.08 1.51 1.16 1.22 -10.61 -0.68 -1.35 0.26 -1.25 -9.68 -0.06 -0.81 3.42 -0.76
Cell Periphery 2.34 -4.76 -3.68 -5.62 1.13 -5.75 -9.16 -2.89 0.72 0.34 -2.49 -6.52 -6.62 -3.22 -1.97
Cytoplasm -3.20 -1.51 7.22 8.23 7.56 8.81 -5.47 2.65 -0.13 4.55 4.12 -8.04 9.18 6.91 13.35
Cytoplasmic Foci -7.83 20.77 13.82 15.79 -1.07 -6.13 27.09 18.07 23.48 -1.26 -9.98 34.97 20.24 26.41 -2.71
Eisosomes 0.44 -2.04 -10.60 -10.54 -9.24 -7.40 -0.21 -4.69 1.06 -4.66 -5.14 0.12 -12.38 -8.02 -12.72
Endoplasmic Reticulum 3.29 -15.00 -11.40 -12.54 3.42 -0.54 -17.05 -5.20 -5.09 0.70 1.27 -20.14 -12.34 -12.68 -1.81
Endosome 2.06 23.10 26.90 12.52 5.28 -14.09 22.00 21.49 33.50 -0.92 -6.63 32.19 33.21 35.42 1.70
Golgi -2.22 5.49 5.87 3.12 5.32 -8.38 3.88 3.69 14.42 -1.56 -9.21 6.26 6.38 13.58 0.65
Lipid Particles 3.32 -0.05 3.03 -1.03 2.42 -3.40 4.37 2.70 5.81 -1.26 -1.33 3.94 2.96 3.91 -1.46
Mitochondria -0.91 3.96 4.52 2.29 7.42 -7.24 2.59 3.06 9.93 1.28 -7.08 4.42 5.75 10.55 4.19
Mitotic Spindle -2.51 -2.89 -3.16 2.15 2.36 -2.26 -2.10 -2.55 -2.36 -2.38 -3.99 -3.97 -2.54 -1.45 -1.52
None -0.79 -3.65 -1.02 0.21 0.76 -3.35 -12.11 -2.33 -1.75 -0.76 -2.47 -11.10 -2.70 -1.31 0.80
Nuclear Periphery -0.18 -13.50 -10.77 -10.73 4.63 2.34 -22.85 -7.93 -8.15 0.66 1.91 -25.25 -13.19 -13.47 3.43
Nuclear Periphery Foci -4.05 -8.84 -10.89 -10.16 4.39 0.70 -8.29 -6.23 -6.27 -3.39 -0.75 -11.02 -12.45 -12.32 -0.59
Nucleolus 1.43 -0.63 5.80 3.41 2.21 -2.73 4.26 4.49 6.19 1.06 -1.66 1.48 6.64 6.55 2.35
Nucleus -2.05 -31.36 -11.88 -11.02 9.94 9.88 -50.28 -4.12 -6.08 17.49 6.93 -60.83 -11.81 -13.13 17.70
Peroxisomes -2.87 5.62 4.56 3.82 -0.04 -7.62 5.98 6.37 16.24 2.20 -7.38 8.41 6.13 11.47 -0.54
Vacuole 6.97 17.54 -5.02 -8.77 -14.32 3.53 31.11* -2.68 -3.60 -10.28 8.25 38.03 -6.49 -9.79 -21.03
Vacuole Periphery 2.01 13.59 14.95 5.15 5.20 -13.83 14.21 13.56 23.29 -0.08 -8.21 18.72 19.61 23.76 4.53
Standard name
Human Ortholog
Description Molecular chaperone of the endoplasmic reticulum lumen; involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway
Localization
Cell Percentages vacuole (87%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Lhs1

Lhs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lhs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available