Standard name
Human Ortholog
Description Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0.05 0 0.05 0.05 0 0 0 0.06 0.1 0.16 0.1 0.08 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.38 0 0.13 0.1 0.08 0.41 0.38 0.39 0.6 0.65 0.61 0.72 0 0 0 0.08 0.08 0 0 0 0 0 0 0
Nucleus 0.93 0.95 0.89 0.91 0.85 0.79 0.72 0.74 0.62 0.63 0.65 0.6 0.92 0.87 0.79 0.84 0.86 0.91 0.86 0.92 0.82 0.84 0.68 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.06 0.06 0.05 0.06 0.09 0.08 0.05 0.1 0.13 0.08 0.1 0 0 0.05 0 0 0 0 0 0.07 0 0.15 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0.06 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 0 3 1 2 4 4 1 2 0 0 0 0 0 0 0 1 1 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 1 0 0 0 1 5 8 7 10 16 12 14 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 2 1 3 4 12 14 16 21 15 16 13 8 11 27 41 22 12 3 0 0 0 0 2 1
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 1 0 0 2 0 1 1 4 2 0 0 0 0 1 0 1
Endosome 0 1 0 0 4 0 4 0 0 0 0 0 0 0 2 2 1 1 1 1 2 0 3 6
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 0 0 1 0 1
Mitochondria 47 7 25 15 19 119 149 174 194 291 183 198 6 1 5 16 12 1 1 0 2 1 1 3
Nucleus 116 178 168 135 199 231 281 328 201 284 193 164 162 244 203 177 132 53 112 175 163 57 113 105
Nuclear Periphery 0 0 0 3 2 0 2 4 2 3 3 4 0 2 1 0 2 0 0 0 0 0 0 0
Nucleolus 14 11 12 8 15 27 30 20 32 59 24 28 5 5 12 4 1 1 3 5 13 1 24 22
Peroxisomes 0 0 0 0 0 0 0 1 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 1
SpindlePole 1 1 0 0 0 0 2 4 2 3 3 2 0 0 1 2 2 0 1 0 0 1 3 4
Vac/Vac Membrane 1 5 5 2 5 6 12 7 9 3 2 9 2 7 4 8 5 1 7 5 9 1 9 23
Unique Cell Count 125 187 188 148 233 292 392 444 324 449 298 274 176 281 257 210 153 58 131 191 198 69 166 182
Labelled Cell Count 182 205 213 168 257 405 505 568 470 680 434 432 186 288 272 236 170 60 131 191 198 69 166 182


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.9 5.4 5.3 5.5 4.3 4.0 4.2 3.9 3.5 3.7 3.5 6.1 7.5 5.7 6.3 7.4 8.2 6.9 6.3 6.7
Std Deviation (1e-4) 0.9 0.9 1.0 1.5 2.1 1.1 1.3 1.5 1.2 0.9 1.3 0.8 1.7 18.0 1.5 1.8 2.0 1.9 3.1 2.3 1.7
Intensity Change (Log2) – – – -0.03 0.01 -0.34 -0.43 -0.36 -0.48 -0.62 -0.57 -0.63 0.17 0.47 0.06 0.22 0.45 0.59 0.34 0.21 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.0 1.9 1.6 1.9 1.8 1.3 1.7 0 2.3 3.5 5.0 3.6 2.8 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.9 -1.7 0 0 0 0 0 0 0 -3.4 -6.0 -4.7 -1.9 -1.6 -2.5
Nucleus 0.6 -1.2 -2.9 -4.8 -4.3 -6.6 -6.6 -6.0 -6.9 0.9 -0.8 -2.9 -1.5 -0.9 0.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.4 0 0 0 0 0 0 0 0 -1.6 -2.6 -0.8 -2.3 -2.7 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0.3 0 0.1 0 0 0.4 0 0 0 0.6 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7679 1.4092 0.9173 0.851 0.5032 1.148 2.1294 2.5869 2.3538 1.9251 1.6853 2.2337 3.0553 3.3247 3.0534 3.1451 2.8528 3.0225
Actin 0.0072 0 0.003 0 0.0082 0 0.0259 0 0.0137 0.0284 0.0116 0.0003 0.0261 0 0.0007 0 0.001 0.0001
Bud 0 0 0 0 0.0006 0 0.0005 0 0.0003 0.0005 0.0001 0 0.0003 0 0 0 0.0003 0
Bud Neck 0.0001 0.0001 0.0002 0.0002 0.0017 0.0011 0.0046 0.0001 0.001 0.0013 0.0004 0.0007 0.0022 0.0001 0.0007 0.0002 0.0021 0.0008
Bud Periphery 0.0001 0 0.0001 0 0.0023 0 0.001 0 0.0006 0.001 0.0001 0 0.0006 0 0 0 0.0013 0
Bud Site 0.0002 0 0.0002 0 0.0009 0.0001 0.0095 0 0.0034 0.0009 0.0004 0.0001 0.001 0 0.0002 0 0.0009 0
Cell Periphery 0 0 0 0 0.0003 0 0.0006 0 0.0001 0.0002 0 0 0.0001 0 0 0 0.0006 0
Cytoplasm 0.0004 0.0001 0.0008 0.0009 0.0005 0.0002 0.0125 0.0042 0.0006 0.0028 0.0014 0.0009 0.001 0.0001 0.0007 0.0002 0.0005 0.0001
Cytoplasmic Foci 0.0042 0 0.0037 0 0.0015 0 0.0118 0.0001 0.008 0.0127 0.0041 0.0002 0.003 0 0.0027 0 0.0003 0.0001
Eisosomes 0.0001 0 0.001 0 0.0003 0 0.0005 0 0.0001 0.0004 0.0001 0 0.0005 0 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0004 0 0.0001 0.0001 0.0013 0 0.0031 0.0002 0.0007 0.0026 0.0001 0.0003 0.0013 0 0.0002 0 0.0013 0.0001
Endosome 0.0039 0 0.0027 0.0001 0.0057 0 0.0138 0.0003 0.0051 0.0056 0.0033 0.0022 0.0074 0 0.0066 0 0.0033 0
Golgi 0.0029 0 0.0041 0 0.0032 0 0.0058 0 0.0037 0.0029 0.0164 0.0003 0.0056 0 0.0013 0 0.0009 0
Lipid Particles 0.0226 0 0.0071 0 0.0118 0 0.0152 0 0.0186 0.0072 0.0015 0.0018 0.0061 0 0.0041 0 0.0022 0
Mitochondria 0.0098 0.0001 0.0088 0.0002 0.0185 0.0001 0.0076 0.0001 0.005 0.0045 0.0021 0.0005 0.0045 0.0001 0.0003 0.0001 0.0055 0.0002
None 0.0006 0 0.006 0.0007 0.001 0.0002 0.0149 0.0021 0.0011 0.0072 0.0311 0.0026 0.0057 0.0001 0.0059 0.0006 0.0023 0
Nuclear Periphery 0.0043 0.0005 0.0018 0.003 0.0164 0.0004 0.0125 0.0035 0.0056 0.0095 0.0035 0.0073 0.0095 0.0014 0.0011 0.001 0.0039 0.0011
Nucleolus 0.0167 0.0108 0.0089 0.0138 0.0485 0.034 0.0168 0.0163 0.0193 0.0046 0.028 0.0341 0.0227 0.0118 0.0089 0.0086 0.0426 0.0397
Nucleus 0.9195 0.9875 0.9199 0.9779 0.8647 0.9618 0.8028 0.9509 0.8739 0.8858 0.8839 0.9436 0.8926 0.9858 0.9572 0.9879 0.9086 0.9468
Peroxisomes 0.0044 0 0.014 0 0.0019 0 0.0117 0 0.0141 0.0175 0.0022 0.0001 0.0014 0 0.0005 0 0.0003 0
Punctate Nuclear 0.0014 0.0008 0.0171 0.0029 0.0055 0.002 0.0249 0.0221 0.0225 0.0019 0.0097 0.0044 0.0067 0.0005 0.0081 0.0014 0.0183 0.0109
Vacuole 0.0003 0 0.0003 0.0001 0.0021 0.0001 0.0027 0.0001 0.0015 0.0015 0.0001 0.0003 0.0009 0 0.0003 0 0.0032 0.0001
Vacuole Periphery 0.0009 0 0.0002 0 0.0033 0 0.0011 0 0.0009 0.0009 0.0001 0.0003 0.0009 0 0.0001 0 0.0007 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.8421 11.8315 7.8553 14.926 15.3538 14.6204 11.3332 10.1989 10.6475 15.8507
Translational Efficiency 1.7276 1.6631 2.148 1.3833 1.3268 1.184 1.553 1.3754 1.4981 1.1631

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1115 1318 671 214 1788 1881 1486 2627 2903 3199 2157 2841

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 659.54 657.60 1015.03 926.30 751.58 763.66 973.87 946.31 716.23 719.96 986.67 944.80
Standard Deviation 77.54 107.52 114.04 138.58 90.79 108.72 125.21 162.50 96.90 120.16 123.32 160.90
Intensity Change Log 2 — -0.004250 0.621990 0.490019 — 0.023004 0.373803 0.332386 — 0.010330 0.495130 0.408210

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000150 0.000594 0.000797 0.001211 0.000188 0.000329 0.000845 0.000552 0.000173 0.000438 0.000830 0.000602
Bud Neck 0.001404 0.005511 0.016088 0.007111 0.003153 0.007816 0.011200 0.029987 0.002481 0.006866 0.012720 0.028264
Bud Site 0.000312 0.001252 0.001236 0.001188 0.000402 0.001468 0.001462 0.004904 0.000368 0.001379 0.001391 0.004624
Cell Periphery 0.000139 0.000274 0.000038 0.000068 0.000079 0.000107 0.000075 0.000228 0.000102 0.000176 0.000063 0.000216
Cytoplasm 0.010704 0.016135 0.001079 0.004035 0.003914 0.004209 0.003559 0.019812 0.006522 0.009123 0.002787 0.018624
Cytoplasmic Foci 0.003041 0.001856 0.000048 0.000089 0.000488 0.001077 0.000075 0.000614 0.001469 0.001398 0.000067 0.000574
Eisosomes 0.000018 0.000025 0.000031 0.000035 0.000015 0.000021 0.000031 0.000031 0.000016 0.000022 0.000031 0.000031
Endoplasmic Reticulum 0.000715 0.003972 0.004282 0.006414 0.001993 0.003449 0.004777 0.002864 0.001502 0.003665 0.004623 0.003131
Endosome 0.000903 0.000869 0.000166 0.000220 0.000239 0.000836 0.000398 0.000837 0.000494 0.000850 0.000326 0.000790
Golgi 0.000546 0.000780 0.000078 0.000109 0.000060 0.000579 0.000085 0.000143 0.000247 0.000662 0.000083 0.000140
Lipid Particles 0.001121 0.000682 0.000114 0.000196 0.000337 0.000474 0.000175 0.000256 0.000638 0.000560 0.000156 0.000251
Mitochondria 0.001987 0.001628 0.000861 0.000984 0.001122 0.003686 0.001283 0.002691 0.001454 0.002838 0.001152 0.002563
Mitotic Spindle 0.001385 0.001508 0.000199 0.000511 0.000757 0.005336 0.004195 0.008800 0.000998 0.003759 0.002952 0.008176
None 0.017771 0.042628 0.001250 0.002819 0.006768 0.005083 0.002901 0.013837 0.010994 0.020552 0.002388 0.013007
Nuclear Periphery 0.000289 0.000503 0.000251 0.000381 0.000444 0.000925 0.000478 0.001140 0.000385 0.000751 0.000407 0.001083
Nuclear Periphery Foci 0.000195 0.001055 0.000076 0.000357 0.000350 0.000443 0.000167 0.000406 0.000290 0.000695 0.000139 0.000402
Nucleolus 0.031197 0.038929 0.013838 0.011821 0.023200 0.024981 0.008453 0.007454 0.026272 0.030728 0.010128 0.007783
Nucleus 0.926335* 0.879688* 0.957909* 0.960142* 0.955606* 0.935935* 0.956901* 0.901739* 0.944363* 0.912761* 0.957214* 0.906139*
Peroxisomes 0.001260 0.000867 0.000277 0.000429 0.000296 0.001389 0.000231 0.000236 0.000666 0.001174 0.000245 0.000250
Vacuole 0.000370 0.000936 0.001274 0.001744 0.000491 0.001224 0.002409 0.002717 0.000444 0.001105 0.002056 0.002644
Vacuole Periphery 0.000155 0.000308 0.000108 0.000136 0.000098 0.000633 0.000300 0.000750 0.000120 0.000499 0.000240 0.000704

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.15 -8.90 -8.52 -6.25 -3.10 -5.16 -19.22 -15.17 -9.64 8.80 -7.06 -18.60 -12.97 -8.07 8.33
Bud Neck -8.37 -12.44 -6.12 -3.05 4.57 -6.69 -16.68 -21.89 -17.11 -12.60 -9.61 -20.92 -22.84 -18.59 -11.07
Bud Site -4.51 -5.39 -3.50 -0.92 0.21 -3.60 -4.50 -7.74 -5.31 -4.04 -5.18 -5.93 -8.02 -5.77 -4.43
Cell Periphery -3.08 4.23 2.72 5.21 -1.82 -1.27 0.36 -5.56 -3.60 -8.78 -3.34 3.03 -4.22 0.26 -9.77
Cytoplasm -2.18 5.59 3.56 5.74 -1.22 -0.14 0.80 -9.22 -9.50 -10.54 -2.19 4.72 -6.68 -4.77 -11.21
Cytoplasmic Foci 1.85 4.55 4.56 4.10 0.05 -1.74 2.99 0.54 2.21 -3.19 0.53 5.31 3.81 3.69 -3.11
Eisosomes -3.54 -7.34 -5.72 -4.19 -1.58 -3.91 -13.96 -10.59 -6.06 2.56 -5.39 -15.18 -10.99 -6.42 2.30
Endoplasmic Reticulum -14.93 -14.49 -9.60 -4.52 -2.97 -6.84 -14.43 -7.65 1.33 9.19 -13.92 -20.42 -14.41 1.10 8.56
Endosome 0.08 1.30 1.27 3.04 -0.30 -3.04 -1.27 -2.48 0.68 -1.59 -1.41 0.73 -0.55 1.07 -1.91
Golgi -0.55 1.85 1.76 1.95 -1.48 -2.39 -5.03 -3.80 2.00 -1.62 -1.97 1.52 1.13 2.75 -1.80
Lipid Particles 1.44 3.00 2.71 3.41 -1.37 -0.76 2.10 1.53 2.62 -0.45 0.86 3.58 3.08 3.90 -0.69
Mitochondria 0.53 1.45 1.01 1.56 -1.42 -2.80 -0.91 -2.59 1.41 -2.89 -2.27 0.29 -1.96 0.83 -3.43
Mitotic Spindle -0.21 1.29 0.86 1.56 -0.62 -3.30 -3.38 -6.56 -2.08 -2.21 -3.15 -2.92 -6.47 -3.47 -3.39
None -5.85 7.78 7.16 11.42 -1.70 1.36 3.63 -2.72 -5.11 -8.00 -4.97 8.15 0.71 5.81 -8.46
Nuclear Periphery -3.31 -1.03 -2.48 0.06 -1.93 -3.10 -4.04 -5.71 -1.73 -4.17 -3.86 -4.35 -6.25 -2.98 -4.88
Nuclear Periphery Foci -4.80 1.05 -1.19 2.21 -1.68 -0.56 1.85 -0.13 0.36 -1.58 -3.72 2.03 -0.70 2.22 -2.00
Nucleolus -2.69 5.53 7.76 11.33 2.35 -0.90 9.81 10.72 10.84 0.70 -2.73 11.27 14.22 17.19 3.16
Nucleus 6.14 -4.21 -4.81 -11.21 -1.36 4.65 1.72 12.68 6.83 11.36 7.93 -1.82 9.27 1.22 11.71
Peroxisomes 1.06 2.33 2.07 1.66 -1.63 -2.39 1.18 1.51 2.62 0.61 -1.36 2.57 2.74 3.28 0.97
Vacuole -2.23 -4.70 -3.65 -2.69 -1.06 -1.47 -4.49 -4.93 -3.30 0.81 -2.09 -5.57 -6.11 -4.35 0.20
Vacuole Periphery -2.37 0.68 0.22 3.05 -0.92 -3.74 -1.33 -2.22 0.32 -0.91 -4.31 -1.20 -2.12 -0.11 -1.21
Standard name
Human Ortholog
Description Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Mud2

Mud2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mud2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available