ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.98 0.97 0.92 0.8 0.6 0.68 0.59 0.53 0.49 0.41 0.99 0.99 0.99 0.97 0.96 0.96 0.92 0.85 0.83 0.94 0.94 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0.29 0.12 0.47 0.64 0.53 0.55 0.71 0.75 0.75 0 0 0 0 0 0 0 0 0.08 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 1 0 0 0 1 0 0 0 0 0 0 1 0 0 1 0 1 0 0 3
Bud 3 1 1 0 0 1 7 5 4 11 4 5 0 1 0 1 0 0 0 0 0 0 1 10
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 4 3 0 1 3 3 6 5 4 2 1 5 13 17 26 20 11 0 0 0 0 0 0
Cytoplasm 377 170 179 60 117 99 131 170 158 142 75 81 425 579 566 270 351 241 368 158 162 264 483 470
Endoplasmic Reticulum 3 0 1 0 2 0 2 1 4 3 1 7 5 2 3 8 16 5 5 5 10 1 3 8
Endosome 0 0 0 0 3 0 1 1 0 2 0 1 0 1 0 1 0 3 6 3 1 0 4 5
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 1 3 1 0 0 0 1 1 1 0 0 1
Mitochondria 22 6 3 18 15 58 140 134 145 190 115 147 0 0 0 4 7 1 8 4 14 4 7 18
Nucleus 1 0 1 0 0 0 0 0 0 0 1 1 1 2 0 0 0 2 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 1 1 0 0 0 0 4 0 0 0 0 1 0 0 1 0 0 0 3
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 2 1 0 0 0 1 0 0 0 0 1 0 0 0 0 2
Vac/Vac Membrane 0 0 1 0 1 2 2 5 4 5 3 5 0 0 4 3 9 5 2 5 1 3 4 9
Unique Cell Count 389 172 183 62 127 124 219 251 266 266 153 197 431 584 572 279 367 250 403 186 195 281 515 543
Labelled Cell Count 410 181 192 78 140 164 288 322 323 358 201 252 437 602 592 314 404 268 403 186 195 281 515 543


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 7.2 6.2 5.9 5.2 4.4 4.3 4.2 4.0 4.1 3.7 3.9 7.7 7.7 7.4 8.4 8.0 7.5 7.8 8.1 8.2
Std Deviation (1e-4) 0.5 0.9 0.7 2.0 0.7 0.9 1.4 0.9 0.9 1.2 0.9 1.1 1.0 1.1 1.0 1.2 1.3 1.0 1.2 1.4 1.5
Intensity Change (Log2) – – – -0.06 -0.25 -0.47 -0.51 -0.56 -0.61 -0.6 -0.72 -0.66 0.33 0.33 0.26 0.44 0.38 0.28 0.35 0.39 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 3.3 2.1 1.6
Cytoplasm -0.5 -2.4 -5.3 -9.0 -7.8 -9.2 -10.2 -10.4 -11.9 0.7 1.5 1.2 -0.7 -1.3 -0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 2.4 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.7 3.8 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7591 3.5441 2.5056 2.4517 2.2233 2.7032 2.2032 2.6585 2.23 2.0252 2.1566 2.3437 2.5439 2.7734 2.625 2.7883 2.4363 2.5209
Actin 0.0055 0.0013 0.0073 0.0429 0.0087 0.0019 0.03 0.0036 0.0127 0.0613 0.0149 0.0063 0.0394 0.0012 0.022 0.0213 0.0305 0.0149
Bud 0.0009 0.0002 0.0008 0.0018 0.0001 0.0001 0.0017 0.0017 0.0032 0.0015 0.0042 0.0004 0.0048 0.0091 0.0111 0.0008 0.0003 0.005
Bud Neck 0.0035 0.0001 0.0004 0.0007 0.0003 0.0005 0.0068 0.0002 0.0005 0.0008 0.0006 0.0016 0.0128 0.0003 0.0046 0.0025 0.0004 0.0025
Bud Periphery 0.0011 0.0001 0.0005 0.0019 0 0.0001 0.0086 0.0008 0.004 0.0011 0.0072 0.0005 0.0046 0.0029 0.0055 0.0011 0.0003 0.0056
Bud Site 0.0018 0.0006 0.0015 0.0117 0.0004 0.0002 0.0042 0.0104 0.0035 0.0162 0.0033 0.0005 0.0272 0.0062 0.0136 0.0013 0.0009 0.0015
Cell Periphery 0.0002 0.0002 0.0002 0.0003 0.0001 0.0001 0.0005 0.0001 0.0002 0.0002 0.0003 0.0001 0.0013 0.0005 0.0013 0.0002 0 0.0002
Cytoplasm 0.682 0.8744 0.7548 0.804 0.7905 0.8824 0.6087 0.8795 0.8061 0.8001 0.6715 0.8696 0.5762 0.8649 0.8066 0.769 0.7864 0.8316
Cytoplasmic Foci 0.0286 0.0077 0.0292 0.0224 0.0351 0.0125 0.0528 0.0108 0.0249 0.021 0.0463 0.0182 0.0609 0.0203 0.0121 0.0286 0.0323 0.0356
Eisosomes 0.0002 0 0.0001 0.0001 0 0 0.0003 0 0.0003 0.0004 0.0002 0 0.0005 0 0.0003 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0222 0.0056 0.0048 0.0043 0.0051 0.0043 0.0139 0.0041 0.0051 0.0087 0.0071 0.0041 0.0148 0.0031 0.0057 0.0101 0.002 0.0025
Endosome 0.0291 0.007 0.0195 0.0516 0.0143 0.0143 0.0767 0.0083 0.0315 0.0276 0.0778 0.0326 0.0463 0.0184 0.0168 0.0485 0.0378 0.0222
Golgi 0.0084 0.0021 0.0107 0.0045 0.0019 0.0021 0.0213 0.0009 0.0081 0.0072 0.024 0.0069 0.0223 0.0041 0.0047 0.0116 0.0224 0.0123
Lipid Particles 0.0045 0.0002 0.0031 0.0016 0.0037 0.0033 0.0157 0.0001 0.002 0.0071 0.0098 0.0042 0.0088 0.0003 0.0012 0.0044 0.0061 0.0029
Mitochondria 0.0067 0.0008 0.0129 0.0023 0.0002 0.0022 0.0168 0.0004 0.0175 0.0043 0.054 0.0013 0.0099 0.0008 0.008 0.0176 0.0054 0.0044
None 0.192 0.0961 0.1376 0.0359 0.0784 0.0679 0.089 0.075 0.0636 0.0263 0.0241 0.0448 0.127 0.0604 0.0789 0.0509 0.0476 0.0423
Nuclear Periphery 0.0033 0.0008 0.001 0.0011 0.0015 0.0004 0.021 0.0007 0.0017 0.001 0.0264 0.0009 0.0056 0.0007 0.0008 0.0054 0.0012 0.0003
Nucleolus 0.0003 0 0.0002 0.0001 0.0005 0 0.0008 0 0.0004 0.0001 0.0006 0.0001 0.0008 0.0001 0.0002 0.0002 0.0001 0.0001
Nucleus 0.0012 0.0005 0.0009 0.0009 0.0013 0.0005 0.0063 0.0009 0.0021 0.001 0.0043 0.0011 0.0027 0.0014 0.002 0.002 0.0008 0.001
Peroxisomes 0.0025 0.0001 0.0083 0.0015 0.0087 0.0048 0.01 0.0003 0.0026 0.0086 0.0064 0.0007 0.0203 0.0023 0.0015 0.0023 0.0207 0.0122
Punctate Nuclear 0.0016 0.0002 0.0026 0.0006 0.0469 0.0004 0.0059 0.0004 0.004 0.0005 0.0023 0.0006 0.0053 0.0005 0.0007 0.0016 0.0016 0.0008
Vacuole 0.0036 0.0015 0.0028 0.0078 0.0017 0.0015 0.0057 0.0015 0.0047 0.0039 0.0086 0.0044 0.0066 0.0022 0.002 0.0106 0.0022 0.0017
Vacuole Periphery 0.001 0.0002 0.0006 0.0019 0.0003 0.0005 0.0033 0.0002 0.0012 0.0013 0.0061 0.0009 0.0018 0.0004 0.0004 0.01 0.001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.563 9.4774 11.9014 11.7595 16.3891 9.7809 12.8996 13.9302 14.2832 14.5247
Translational Efficiency 1.6263 1.6556 1.0918 1.6772 0.8613 1.5118 1.18 1.0818 1.0332 0.97

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1474 136 1391 1467 1460 2532 369 800 2934 2668 1760 2267

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 732.55 994.00 989.93 1037.71 686.05 857.51 1093.14 963.33 709.41 864.47 1011.57 1011.46
Standard Deviation 92.24 138.27 121.96 131.35 105.91 100.97 139.81 169.80 101.96 107.48 132.74 150.34
Intensity Change Log 2 — 0.440319 0.434399 0.502404 — 0.321840 0.672093 0.489716 — 0.384234 0.554243 0.496282

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000215 0.000700 0.000145 0.001690 0.000160 0.000235 0.000200 0.003616 0.000187 0.000259 0.000156 0.002370
Bud Neck 0.012220 0.008381 0.001705 0.004027 0.004802 0.011211 0.001890 0.006816 0.008529 0.011066 0.001744 0.005011
Bud Site 0.019419 0.036362 0.005758 0.028535 0.013340 0.021380 0.012632 0.029398 0.016394 0.022144 0.007199 0.028839
Cell Periphery 0.000311 0.000125 0.000056 0.000121 0.000437 0.000068 0.000122 0.000968 0.000374 0.000071 0.000069 0.000420
Cytoplasm 0.367762* 0.435210* 0.725461* 0.611981* 0.516068* 0.531866* 0.545763* 0.470869* 0.441561* 0.526939* 0.687786* 0.562184*
Cytoplasmic Foci 0.363141* 0.334933* 0.034532 0.063179 0.247526* 0.259338* 0.051585 0.073016 0.305610* 0.263191* 0.038108 0.066650
Eisosomes 0.000242 0.000061 0.000015 0.000023 0.000071 0.000053 0.000058 0.002591 0.000157 0.000053 0.000024 0.000929
Endoplasmic Reticulum 0.007529 0.001749 0.001588 0.002767 0.004629 0.001073 0.001613 0.001322 0.006086 0.001108 0.001593 0.002257
Endosome 0.037401 0.041445 0.005184 0.010834 0.029192 0.025247 0.006740 0.016416 0.033316 0.026073 0.005511 0.012804
Golgi 0.043066 0.005870 0.000273 0.004943 0.005716 0.014692 0.000212 0.008068 0.024480 0.014243 0.000260 0.006046
Lipid Particles 0.011342 0.002222 0.001064 0.002384 0.011346 0.002581 0.002605 0.004209 0.011344 0.002563 0.001387 0.003028
Mitochondria 0.025289 0.002906 0.000832 0.002455 0.003607 0.005246 0.000086 0.002245 0.014500 0.005127 0.000676 0.002381
Mitotic Spindle 0.001618 0.000951 0.005634 0.044175 0.000534 0.011073 0.001118 0.026938 0.001079 0.010557 0.004687 0.038092
None 0.002709 0.000286 0.002622 0.001047 0.005269 0.000689 0.005861 0.006282 0.003983 0.000668 0.003301 0.002894
Nuclear Periphery 0.000168 0.000087 0.000880 0.000726 0.000814 0.000120 0.000815 0.000830 0.000489 0.000118 0.000866 0.000763
Nuclear Periphery Foci 0.000256 0.001105 0.002547 0.004168 0.001377 0.000254 0.006277 0.002862 0.000814 0.000297 0.003329 0.003707
Nucleolus 0.000572 0.000252 0.000275 0.000429 0.001712 0.000386 0.000335 0.002289 0.001139 0.000379 0.000288 0.001085
Nucleus 0.015307 0.012551 0.099405 0.037684 0.045059 0.014818 0.110572 0.129892 0.030112 0.014702 0.101746 0.070223
Peroxisomes 0.003210 0.008714 0.000305 0.001349 0.001764 0.003722 0.000358 0.002813 0.002491 0.003976 0.000316 0.001866
Vacuole 0.069479 0.102506 0.111214 0.172771 0.100583 0.090421 0.250133* 0.203628* 0.084957 0.091037 0.140340 0.183660
Vacuole Periphery 0.018743 0.003583 0.000504 0.004714 0.005994 0.005527 0.001025 0.004931 0.012399 0.005428 0.000613 0.004790

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.43 2.31 -4.03 -1.01 -4.38 -2.51 -4.22 -2.26 -2.17 -2.09 -2.73 0.40 -3.67 -3.44 -3.70
Bud Neck 3.05 14.69 11.22 3.72 -4.00 -8.08 7.27 0.31 6.52 -3.19 -3.17 15.57 8.89 8.95 -5.06
Bud Site -2.47 9.64 0.40 2.56 -7.27 -3.98 1.53 -2.53 -0.20 -3.37 -3.66 7.78 -1.90 1.16 -8.01
Cell Periphery 4.57 7.86 6.84 1.38 -5.06 3.14 2.73 -0.06 -2.81 -2.45 4.98 5.02 2.05 -2.99 -3.04
Cytoplasm -2.77 -33.54 -14.55 -3.24 16.31 -1.83 2.46 6.68 8.46 2.51 -11.53 -24.28 -5.49 4.95 17.06
Cytoplasmic Foci 1.82 57.90 53.39 14.58 -6.46 -1.68 36.30 30.96 37.16 -5.41 7.72 66.07 58.97 46.54 -8.78
Eisosomes 9.76 15.37 14.93 3.09 -5.08 4.96 3.54 -1.85 -1.88 -1.88 13.14 17.05 -1.27 -1.79 -1.92
Endoplasmic Reticulum 4.24 5.93 5.78 0.09 -0.16 5.95 4.52 5.94 0.05 4.25 8.44 7.43 7.72 -1.39 0.88
Endosome -0.38 21.33 19.24 4.76 -2.56 2.53 17.22 9.93 9.11 -4.10 5.43 27.13 21.59 16.03 -4.67
Golgi 17.97 24.60 22.31 2.82 -2.87 -11.12 14.33 1.70 8.01 -2.88 8.86 25.25 19.65 13.16 -4.05
Lipid Particles 10.70 14.16 11.38 -1.08 -6.65 11.18 11.49 8.76 -0.81 -1.46 15.99 18.85 14.63 -1.02 -5.84
Mitochondria 9.22 15.52 15.01 0.88 -0.61 -2.05 6.27 3.21 5.26 -1.68 9.43 15.53 14.54 6.19 -1.04
Mitotic Spindle 1.16 -2.93 -10.90 -11.16 -9.67 -7.02 -2.68 -8.04 -6.00 -7.59 -6.59 -3.85 -13.62 -9.85 -12.07
None 2.55 0.29 1.33 -8.55 3.95 5.37 0.59 1.00 -2.32 0.55 5.17 1.62 2.21 -3.34 1.19
Nuclear Periphery 2.14 -12.22 -13.04 -15.36 -0.79 6.25 -2.87 -0.66 -4.29 1.05 5.83 -7.50 -5.76 -10.89 0.09
Nuclear Periphery Foci -2.58 -10.87 -13.10 -6.98 -3.98 7.93 -6.73 -6.50 -9.12 2.36 6.45 -10.90 -13.38 -15.72 -2.21
Nucleolus 1.61 1.48 0.37 -2.95 -2.73 5.33 5.91 0.36 -1.21 -1.36 4.59 5.41 0.97 -1.46 -1.81
Nucleus 0.77 -26.78 -18.82 -14.59 14.93 12.83 -11.41 -6.39 -12.77 4.99 11.19 -25.36 -13.13 -21.43 13.61
Peroxisomes -3.24 16.11 10.19 4.58 -5.85 -6.72 7.11 -0.09 4.96 -4.58 -5.77 15.84 6.15 10.40 -7.11
Vacuole -3.31 -11.90 -28.08 -12.24 -16.81 2.28 -15.23 -19.27 -20.58 0.59 -2.27 -16.24 -33.34 -32.11 -14.73
Vacuole Periphery 10.80 15.76 13.50 1.71 -3.02 0.88 10.54 3.65 3.33 -1.80 9.91 18.69 13.42 6.24 -3.39
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin
Localization
Cell Percentages cytoplasm (85%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YKL075C

YKL075C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ykl075c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available