Standard name | |
---|---|
Human Ortholog | |
Description | Kinesin-like myosin passenger-protein; interacts with Myo2p and enhances its interaction with Sec4p during transport of secretory vesicles; controls actin cable structure and dynamics |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 25 | 32 | 9 | 56 | 69 | 38 | 60 | – | 25 | 25 | 43 | 46 | – | 15 | 19 | 54 | 59 | 35 | 26 | 35 | 13 | 3 | 23 | 35 |
Bud | 20 | 22 | 6 | 31 | 55 | 43 | 47 | – | 26 | 48 | 57 | 60 | – | 6 | 12 | 20 | 21 | 24 | 43 | 38 | 17 | 5 | 76 | 102 |
Bud Neck | 0 | 1 | 0 | 4 | 4 | 1 | 4 | – | 1 | 4 | 1 | 4 | – | 0 | 0 | 0 | 6 | 0 | 11 | 20 | 10 | 2 | 30 | 41 |
Bud Site | 16 | 11 | 3 | 10 | 19 | 26 | 29 | – | 9 | 12 | 11 | 24 | – | 4 | 1 | 6 | 13 | 13 | – | – | – | – | – | – |
Cell Periphery | 1 | 4 | 1 | 7 | 3 | 2 | 5 | – | 10 | 7 | 13 | 11 | – | 2 | 9 | 12 | 32 | 20 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 41 | 52 | 22 | 49 | 100 | 84 | 122 | – | 80 | 103 | 109 | 164 | – | 71 | 84 | 74 | 111 | 96 | 28 | 33 | 18 | 2 | 11 | 39 |
Endoplasmic Reticulum | 1 | 6 | 1 | 7 | 3 | 9 | 12 | – | 10 | 9 | 16 | 14 | – | 5 | 0 | 8 | 9 | 13 | 3 | 3 | 2 | 0 | 0 | 3 |
Endosome | 10 | 12 | 1 | 15 | 14 | 20 | 14 | – | 9 | 16 | 14 | 33 | – | 4 | 1 | 17 | 28 | 51 | 8 | 8 | 6 | 0 | 13 | 9 |
Golgi | 0 | 5 | 0 | 0 | 3 | 2 | 1 | – | 0 | 0 | 4 | 3 | – | 1 | 1 | 17 | 23 | 11 | 2 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 22 | 7 | 2 | 6 | 8 | 33 | 41 | – | 61 | 81 | 145 | 119 | – | 0 | 0 | 24 | 20 | 24 | 4 | 6 | 9 | 0 | 4 | 4 |
Nucleus | 2 | 1 | 1 | 1 | 5 | 3 | 7 | – | 2 | 5 | 0 | 3 | – | 0 | 0 | 1 | 4 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 1 | 1 | 3 | 4 | 13 | – | 3 | 11 | 5 | 6 | – | 0 | 0 | 0 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 15 | 15 | 0 | 17 | 30 | 33 | 22 | – | 11 | 10 | 12 | 21 | – | 7 | 5 | 8 | 30 | 23 | 1 | 1 | 0 | 1 | 4 | 5 |
SpindlePole | 6 | 4 | 3 | 34 | 64 | 45 | 35 | – | 12 | 29 | 17 | 36 | – | 8 | 3 | 19 | 49 | 52 | 1 | 3 | 3 | 2 | 12 | 16 |
Vac/Vac Membrane | 2 | 5 | 3 | 4 | 15 | 9 | 23 | – | 17 | 25 | 27 | 26 | – | 2 | 4 | 34 | 31 | 39 | 2 | 6 | 2 | 1 | 3 | 3 |
Unique Cell Count | 113 | 130 | 41 | 168 | 296 | 242 | 314 | 209 | 290 | 348 | 442 | 107 | 115 | 217 | 322 | 299 | 138 | 162 | 87 | 21 | 182 | 266 | ||
Labelled Cell Count | 161 | 178 | 53 | 242 | 395 | 352 | 435 | 276 | 385 | 474 | 571 | 125 | 139 | 294 | 437 | 412 | 138 | 162 | 87 | 21 | 182 | 266 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 8.2 | 7.1 | 7.5 | 7.9 | 7.1 | 7.1 | – | 6.5 | 6.8 | 6.3 | 6.6 | – | 8.7 | 8.7 | 10.3 | 10.3 | 10.0 | – | 7.4 | 7.6 |
Std Deviation (1e-4) | 0.8 | 1.1 | 1.2 | 1.0 | 1.5 | 1.4 | 1.8 | – | 1.4 | 1.5 | 1.3 | 1.4 | – | 1.1 | 1.4 | 2.0 | 1.9 | 2.2 | – | 1.1 | 1.0 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.17 | 0.01 | 0.02 | – | -0.12 | -0.05 | -0.16 | -0.09 | – | 0.29 | 0.29 | 0.55 | 0.54 | 0.5 | – | 0.06 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.4 | 0.2 | -1.0 | -0.4 | – | -1.7 | 0 | -1.7 | 0 | – | -1.2 | -0.8 | 0.4 | -0.6 | -1.8 |
Bud | 0.6 | 0.6 | 0.5 | 0.1 | – | -0.4 | 0.3 | 0.3 | -0.2 | – | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -3.0 | -2.5 | -2.3 | -1.8 | – | -1.8 | -2.2 | -2.9 | -2.1 | – | 1.4 | 2.3 | -2.4 | -2.4 | -2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2.4 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 1.6 | 0 | – | 3.3 | 3.2 | 4.6 | 3.1 | – | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1.9 | 2.2 | 1.8 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1.4 | 1.6 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 1.4 | 0 | 1.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5869 | 3.3819 | 2.9228 | 3.4572 | 2.3901 | 3.0058 | 3.4741 | 4.2539 | 4.1594 | 3.3851 | 3.7584 | 4.1336 | 1.299 | 1.9264 | 1.7782 | 1.7575 | 1.626 | 2.0494 |
Actin | 0.2129 | 0.052 | 0.176 | 0.2946 | 0.3239 | 0.0361 | 0.1287 | 0.0553 | 0.1824 | 0.0651 | 0.2819 | 0.08 | 0.1119 | 0.0488 | 0.1775 | 0.1461 | 0.2623 | 0.1003 |
Bud | 0.0267 | 0.0203 | 0.1923 | 0.0917 | 0.0454 | 0.0074 | 0.0647 | 0.0573 | 0.2424 | 0.15 | 0.1031 | 0.0127 | 0.0586 | 0.0642 | 0.2323 | 0.1419 | 0.0238 | 0.0098 |
Bud Neck | 0.0387 | 0.0087 | 0.0121 | 0.0092 | 0.0447 | 0.5925 | 0.0302 | 0.0067 | 0.0015 | 0.0018 | 0.0162 | 0.495 | 0.0372 | 0.0091 | 0.0037 | 0.0025 | 0.0021 | 0.4935 |
Bud Periphery | 0.0427 | 0.0115 | 0.2446 | 0.1448 | 0.092 | 0.0071 | 0.0554 | 0.0339 | 0.2972 | 0.3363 | 0.1723 | 0.0056 | 0.0804 | 0.0405 | 0.2714 | 0.2599 | 0.0388 | 0.011 |
Bud Site | 0.0917 | 0.4289 | 0.0951 | 0.0319 | 0.0084 | 0.0122 | 0.1541 | 0.5103 | 0.1062 | 0.0135 | 0.0187 | 0.006 | 0.1875 | 0.4439 | 0.0642 | 0.0304 | 0.0107 | 0.0092 |
Cell Periphery | 0.003 | 0.0016 | 0.0017 | 0.0015 | 0.0004 | 0.0007 | 0.0027 | 0.0023 | 0.0016 | 0.0014 | 0.0026 | 0.0006 | 0.0021 | 0.0014 | 0.0012 | 0.0015 | 0.0007 | 0.0007 |
Cytoplasm | 0.1052 | 0.2767 | 0.1076 | 0.2381 | 0.1568 | 0.1324 | 0.1439 | 0.1965 | 0.0919 | 0.1383 | 0.2258 | 0.1494 | 0.1853 | 0.2181 | 0.12 | 0.1736 | 0.3169 | 0.1356 |
Cytoplasmic Foci | 0.2196 | 0.0895 | 0.0473 | 0.0292 | 0.0636 | 0.1385 | 0.2201 | 0.0938 | 0.0226 | 0.0779 | 0.0474 | 0.1219 | 0.169 | 0.0697 | 0.0339 | 0.0362 | 0.0824 | 0.1454 |
Eisosomes | 0.0013 | 0.0002 | 0.0002 | 0.0004 | 0.0006 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0079 | 0.002 | 0.0062 | 0.0033 | 0.0011 | 0.0027 | 0.0156 | 0.0007 | 0.0019 | 0.003 | 0.0025 | 0.0022 | 0.0068 | 0.0078 | 0.0012 | 0.0057 | 0.0023 | 0.0019 |
Endosome | 0.0483 | 0.0201 | 0.0421 | 0.0603 | 0.0566 | 0.0349 | 0.039 | 0.0053 | 0.0112 | 0.0603 | 0.025 | 0.0356 | 0.0567 | 0.0437 | 0.026 | 0.1015 | 0.0881 | 0.0248 |
Golgi | 0.046 | 0.008 | 0.0241 | 0.021 | 0.093 | 0.017 | 0.0242 | 0.0054 | 0.0081 | 0.0532 | 0.0283 | 0.0366 | 0.0283 | 0.017 | 0.0116 | 0.0275 | 0.0307 | 0.02 |
Lipid Particles | 0.018 | 0.0018 | 0.008 | 0.0033 | 0.0481 | 0.0011 | 0.0131 | 0.0024 | 0.0015 | 0.0307 | 0.0026 | 0.0038 | 0.0095 | 0.0011 | 0.0063 | 0.0024 | 0.0322 | 0.0101 |
Mitochondria | 0.0198 | 0.0017 | 0.0105 | 0.0188 | 0.0205 | 0.0015 | 0.0138 | 0.001 | 0.0057 | 0.0187 | 0.028 | 0.0056 | 0.0046 | 0.0026 | 0.0068 | 0.0095 | 0.0103 | 0.0061 |
None | 0.045 | 0.0311 | 0.0061 | 0.0043 | 0.0011 | 0.0016 | 0.0105 | 0.0015 | 0.0135 | 0.0026 | 0.0275 | 0.0014 | 0.0131 | 0.0084 | 0.0064 | 0.0064 | 0.0219 | 0.0033 |
Nuclear Periphery | 0.0076 | 0.0013 | 0.0059 | 0.0022 | 0.0007 | 0.0003 | 0.0101 | 0.0002 | 0.0005 | 0.0009 | 0.0008 | 0.0018 | 0.0035 | 0.0009 | 0.0011 | 0.0052 | 0.0012 | 0.0005 |
Nucleolus | 0.0015 | 0.0012 | 0.0004 | 0.0027 | 0.0001 | 0.0002 | 0.0013 | 0.0005 | 0.0002 | 0.0002 | 0.0008 | 0.0006 | 0.0035 | 0.0012 | 0.0018 | 0.0005 | 0.001 | 0.0019 |
Nucleus | 0.0038 | 0.0035 | 0.0022 | 0.0026 | 0.0003 | 0.0005 | 0.0177 | 0.001 | 0.0005 | 0.0007 | 0.0012 | 0.0031 | 0.0025 | 0.0038 | 0.0021 | 0.0022 | 0.0012 | 0.0008 |
Peroxisomes | 0.0312 | 0.0079 | 0.01 | 0.0067 | 0.036 | 0.0073 | 0.0383 | 0.0181 | 0.0075 | 0.0281 | 0.0048 | 0.034 | 0.0179 | 0.0078 | 0.02 | 0.006 | 0.0363 | 0.0204 |
Punctate Nuclear | 0.0183 | 0.008 | 0.0021 | 0.0174 | 0.0016 | 0.0023 | 0.0059 | 0.0035 | 0.0006 | 0.0009 | 0.0023 | 0.0018 | 0.0077 | 0.0032 | 0.0064 | 0.0015 | 0.0275 | 0.0026 |
Vacuole | 0.009 | 0.021 | 0.0041 | 0.0132 | 0.0029 | 0.0028 | 0.0085 | 0.0041 | 0.0022 | 0.0112 | 0.0061 | 0.0014 | 0.0117 | 0.0057 | 0.0044 | 0.0315 | 0.0065 | 0.0015 |
Vacuole Periphery | 0.002 | 0.003 | 0.0015 | 0.0028 | 0.0021 | 0.0006 | 0.0017 | 0.0003 | 0.0007 | 0.005 | 0.0019 | 0.0006 | 0.0018 | 0.0011 | 0.0015 | 0.0077 | 0.0026 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.0361 | 21.0187 | 13.74 | 16.1446 | 15.2424 | 11.7497 | 17.71 | 20.3202 | 16.4007 | 16.9946 |
Translational Efficiency | 0.7502 | 0.6038 | 0.8748 | 0.7025 | 0.6106 | 0.9197 | 0.8175 | 0.6639 | 0.744 | 0.7464 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
356 | 1574 | 200 | 1065 | 1775 | 1067 | 106 | 1974 | 2131 | 2641 | 306 | 3039 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 738.98 | 837.90 | 1087.00 | 1011.43 | 770.90 | 768.05 | 1252.85 | 1040.88 | 765.57 | 809.68 | 1144.45 | 1030.56 |
Standard Deviation | 217.58 | 102.68 | 131.03 | 164.46 | 89.61 | 92.57 | 155.79 | 133.29 | 121.40 | 104.50 | 160.80 | 145.66 |
Intensity Change Log 2 | — | 0.181243 | 0.556745 | 0.452789 | — | -0.005343 | 0.700598 | 0.433188 | — | 0.088993 | 0.631982 | 0.442815 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.040969 | 0.016906 | 0.031821 | 0.062580 | 0.022444 | 0.016830 | 0.030560 | 0.045183 | 0.025538 | 0.016875 | 0.031384 | 0.051279 |
Bud Neck | 0.056488 | 0.039470 | 0.031796 | 0.012884 | 0.068752 | 0.061958 | 0.014733 | 0.019093 | 0.066703 | 0.048556 | 0.025885 | 0.016917 |
Bud Site | 0.285761* | 0.320251* | 0.526526* | 0.284759* | 0.366271* | 0.359306* | 0.262509* | 0.305597* | 0.352821* | 0.336030* | 0.435069* | 0.298295* |
Cell Periphery | 0.002508 | 0.000443 | 0.000614 | 0.000559 | 0.000920 | 0.001067 | 0.000850 | 0.000302 | 0.001185 | 0.000695 | 0.000696 | 0.000392 |
Cytoplasm | 0.145803* | 0.249225* | 0.143397* | 0.375786* | 0.200455* | 0.210395* | 0.310086* | 0.483295* | 0.191325* | 0.233537* | 0.201139* | 0.445619* |
Cytoplasmic Foci | 0.159320 | 0.200386* | 0.063938 | 0.104778 | 0.176713 | 0.174011 | 0.157074 | 0.052668 | 0.173807 | 0.189730* | 0.096201 | 0.070930 |
Eisosomes | 0.001290 | 0.000399 | 0.000169 | 0.000207 | 0.000684 | 0.000884 | 0.000148 | 0.000132 | 0.000785 | 0.000595 | 0.000162 | 0.000158 |
Endoplasmic Reticulum | 0.004158 | 0.001292 | 0.001559 | 0.000957 | 0.001043 | 0.000648 | 0.003613 | 0.000234 | 0.001563 | 0.001032 | 0.002271 | 0.000487 |
Endosome | 0.074815 | 0.042213 | 0.019706 | 0.021783 | 0.038687 | 0.047920 | 0.028930 | 0.008955 | 0.044723 | 0.044519 | 0.022901 | 0.013450 |
Golgi | 0.070364 | 0.052920 | 0.001616 | 0.019332 | 0.047625 | 0.038614 | 0.015241 | 0.006879 | 0.051424 | 0.047140 | 0.006335 | 0.011243 |
Lipid Particles | 0.010507 | 0.003250 | 0.002636 | 0.005172 | 0.002732 | 0.002734 | 0.005221 | 0.001519 | 0.004031 | 0.003041 | 0.003531 | 0.002799 |
Mitochondria | 0.033572 | 0.010317 | 0.000144 | 0.002105 | 0.017320 | 0.013866 | 0.000061 | 0.002554 | 0.020035 | 0.011751 | 0.000115 | 0.002396 |
Mitotic Spindle | 0.006905 | 0.005898 | 0.018179 | 0.017107 | 0.003552 | 0.004152 | 0.010707 | 0.010163 | 0.004112 | 0.005193 | 0.015591 | 0.012596 |
None | 0.001691 | 0.000458 | 0.000126 | 0.000180 | 0.000454 | 0.000336 | 0.000062 | 0.000117 | 0.000661 | 0.000409 | 0.000104 | 0.000139 |
Nuclear Periphery | 0.000088 | 0.000027 | 0.000095 | 0.000073 | 0.000028 | 0.000049 | 0.000136 | 0.000023 | 0.000038 | 0.000036 | 0.000109 | 0.000040 |
Nuclear Periphery Foci | 0.000635 | 0.000546 | 0.002711 | 0.000680 | 0.000664 | 0.000855 | 0.015559 | 0.000164 | 0.000660 | 0.000671 | 0.007162 | 0.000345 |
Nucleolus | 0.000902 | 0.000101 | 0.000100 | 0.000326 | 0.000132 | 0.000258 | 0.000113 | 0.000054 | 0.000261 | 0.000165 | 0.000104 | 0.000149 |
Nucleus | 0.010083 | 0.002016 | 0.005079 | 0.002424 | 0.001609 | 0.001123 | 0.006864 | 0.001904 | 0.003025 | 0.001656 | 0.005698 | 0.002086 |
Peroxisomes | 0.070659 | 0.031399 | 0.047851 | 0.032496 | 0.033807 | 0.046108 | 0.017790 | 0.031712 | 0.039963 | 0.037342 | 0.037437 | 0.031987 |
Vacuole | 0.015611 | 0.018597 | 0.101701 | 0.053265 | 0.012971 | 0.015652 | 0.118986 | 0.028271 | 0.013412 | 0.017407 | 0.107689 | 0.037030 |
Vacuole Periphery | 0.007870 | 0.003887 | 0.000236 | 0.002545 | 0.003137 | 0.003234 | 0.000758 | 0.001181 | 0.003928 | 0.003623 | 0.000417 | 0.001659 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 3.50 | 1.56 | -1.69 | -7.48 | -4.03 | 1.98 | -1.03 | -5.62 | -7.05 | -2.22 | 3.68 | -0.74 | -6.56 | -10.44 | -4.24 |
Bud Neck | 2.59 | 3.81 | 7.26 | 9.97 | 3.50 | 1.31 | 10.39 | 14.04 | 10.80 | -0.97 | 4.82 | 9.83 | 16.11 | 13.96 | 2.19 |
Bud Site | -1.95 | -5.73 | 1.63 | 5.45 | 7.51 | 0.61 | 4.49 | 8.13 | 6.27 | -1.45 | 1.85 | -1.47 | 9.03 | 7.53 | 5.37 |
Cell Periphery | 4.35 | 3.94 | 4.20 | -1.16 | 0.91 | -0.57 | 0.65 | 6.08 | 3.18 | 4.82 | 3.25 | 3.71 | 6.92 | 3.17 | 4.09 |
Cytoplasm | -8.28 | -0.73 | -17.30 | -12.30 | -12.38 | -1.10 | -4.40 | -32.13 | -27.77 | -6.88 | -6.09 | -1.49 | -34.86 | -29.34 | -15.86 |
Cytoplasmic Foci | -4.06 | 8.39 | 5.65 | 13.67 | -4.44 | 0.29 | 1.65 | 22.58 | 18.15 | 7.45 | -2.73 | 9.62 | 20.96 | 25.40 | 3.59 |
Eisosomes | 3.89 | 5.04 | 4.98 | 3.38 | -0.45 | -0.76 | 6.34 | 6.63 | 3.08 | 0.88 | 1.45 | 7.77 | 8.04 | 4.22 | 0.84 |
Endoplasmic Reticulum | 2.06 | 1.42 | 2.36 | 1.59 | 3.59 | 1.99 | -3.88 | 4.81 | 3.54 | 5.29 | 1.79 | -2.64 | 3.97 | 4.04 | 7.08 |
Endosome | 3.99 | 6.02 | 6.90 | 7.72 | 0.06 | -2.47 | 2.51 | 13.67 | 12.17 | 5.53 | 0.04 | 5.51 | 13.89 | 15.74 | 3.04 |
Golgi | 2.20 | 9.31 | 7.02 | 11.14 | -6.98 | 2.81 | 3.97 | 17.44 | 13.39 | 0.90 | 1.49 | 12.55 | 17.25 | 18.79 | -1.34 |
Lipid Particles | 2.53 | 2.45 | 1.63 | -2.14 | -1.82 | -0.11 | -3.15 | 2.76 | 1.86 | 4.31 | 1.62 | 0.01 | 1.41 | -0.11 | 1.40 |
Mitochondria | 4.64 | 6.77 | 6.39 | 8.57 | -2.84 | 1.60 | 13.51 | 11.16 | 6.44 | -5.45 | 5.17 | 14.73 | 12.84 | 10.46 | -5.97 |
Mitotic Spindle | 0.48 | -1.39 | -3.15 | -4.21 | -0.14 | -0.65 | -0.74 | -4.57 | -3.80 | -0.06 | -1.37 | -1.94 | -6.23 | -5.35 | 0.25 |
None | 2.30 | 2.82 | 2.81 | 3.70 | -0.16 | 0.84 | 4.53 | 3.85 | 2.16 | -3.67 | 2.00 | 4.34 | 4.45 | 4.66 | -0.30 |
Nuclear Periphery | 2.74 | -2.10 | 0.05 | -3.24 | 2.26 | -0.76 | -3.72 | -0.14 | 0.76 | 3.86 | 0.28 | -4.81 | -0.99 | -1.31 | 4.50 |
Nuclear Periphery Foci | 0.26 | -4.04 | -0.96 | -1.45 | 3.87 | -0.32 | -4.77 | 1.03 | 1.49 | 4.94 | -0.05 | -5.73 | 0.58 | 1.09 | 6.10 |
Nucleolus | 1.48 | 1.44 | 0.94 | -0.90 | -0.83 | -2.06 | 0.48 | 2.77 | 3.21 | 1.02 | 0.93 | 1.52 | 0.78 | 0.12 | -0.47 |
Nucleus | 2.55 | 0.62 | 2.14 | -3.85 | 3.64 | 2.63 | -4.80 | -4.89 | -7.82 | 3.86 | 2.43 | -4.29 | 0.32 | -6.04 | 5.15 |
Peroxisomes | 5.26 | 2.91 | 5.49 | 0.76 | 1.94 | -3.39 | 3.43 | 1.43 | 4.48 | -2.73 | 0.96 | 1.33 | 4.15 | 3.47 | 0.74 |
Vacuole | -1.11 | -8.00 | -12.62 | -13.08 | 4.34 | -1.64 | -7.72 | -12.53 | -10.23 | 6.01 | -3.16 | -10.72 | -18.26 | -16.14 | 7.50 |
Vacuole Periphery | 2.17 | 4.35 | 3.13 | 2.32 | -3.99 | -0.20 | 9.77 | 4.28 | 3.62 | -0.79 | 0.61 | 10.00 | 5.17 | 4.38 | -3.20 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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