Standard name
Human Ortholog
Description Subunit C of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane; the V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase) has eight subunits
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0.05 – 0.08 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.08 0.07 0.06 – 0.09 0.12 0.09 0.05 0 0 0.1 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.06 – 0.05 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.06 0.09 0.06 0.08 0.09 – 0.08 0.07 0.07 0 0.05 0.06 0.2 0.18 0.14 0 0 0 0 0 0
Mitochondria 0 0 0.05 0 0.1 0.15 0.11 0.12 – 0.12 0.2 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.1 0.05 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0.06 0.05 0 0.08 0.09 0.07
Nuclear Periphery 0 0 0.16 0.21 0 0.11 0.15 0.13 – 0.09 0.08 0.15 0 0.15 0.18 0.06 0 0 0 0 0.11 0 0.05 0
Nucleolus 0 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.87 0.92 0.85 0.88 0.86 0.67 0.68 0.6 – 0.65 0.55 0.49 0.92 0.91 0.91 0.85 0.89 0.86 0.86 0.88 0.81 0.77 0.74 0.8
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 10 24 53 – 32 30 30 0 0 2 0 1 0 0 0 0 0 0 0
Bud 0 0 0 0 1 1 4 4 – 8 2 3 1 0 0 0 0 0 0 2 2 0 6 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 4 7 13 56 62 – 78 68 60 0 0 1 1 0 1 0 0 0 0 0 0
Cytoplasm 13 6 12 17 25 72 77 71 – 81 99 72 10 8 8 17 16 15 1 0 1 3 2 1
Endoplasmic Reticulum 0 1 1 2 3 24 31 73 – 45 41 54 1 1 3 4 5 9 0 0 0 0 5 1
Endosome 0 0 1 1 2 0 1 1 – 3 1 0 0 0 0 1 0 0 1 1 4 1 2 3
Golgi 16 4 9 29 53 51 82 102 – 74 59 58 8 10 14 36 39 32 1 0 1 2 4 3
Mitochondria 4 0 21 23 55 131 117 136 – 110 163 193 0 0 0 4 3 6 2 1 11 5 7 3
Nucleus 36 7 14 6 15 40 27 22 – 21 17 12 11 3 3 5 5 10 23 7 7 14 36 23
Nuclear Periphery 8 2 68 105 21 101 163 156 – 79 70 125 7 30 41 11 8 10 9 0 52 6 19 15
Nucleolus 12 5 26 23 10 22 18 34 – 9 8 11 4 2 3 6 6 9 13 3 4 5 11 10
Peroxisomes 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 310 123 356 453 487 596 731 698 – 606 457 399 184 182 207 151 191 198 328 130 374 141 298 282
Unique Cell Count 356 134 419 512 567 893 1079 1167 927 834 816 201 199 228 177 214 230 383 148 462 184 402 352
Labelled Cell Count 399 148 509 664 679 1061 1331 1413 1146 1015 1017 226 236 282 236 274 290 383 148 462 184 402 352


Ambiguous, Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 36.8 34.5 24.4 25.3 23.4 21.3 20.9 20.3 – 19.2 21.1 19.4 29.2 31.9 28.6 36.2 36.9 37.6 33.0 33.5 36.1
Std Deviation (1e-4) 9.7 10.6 7.6 7.2 6.8 7.0 6.6 6.1 – 5.9 5.9 4.9 9.2 10.7 7.9 12.4 13.3 12.7 12.1 13.0 12.2
Intensity Change (Log2) – – – 0.05 -0.06 -0.2 -0.22 -0.27 – -0.35 -0.21 -0.34 0.26 0.38 0.23 0.57 0.6 0.62 0.44 0.46 0.56

WT3RAP60RAP140RAP220RAP300RAP380RAP540RAP620RAP70002040WT3HU80HU120HU16002040WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302040WT1AF100AF140AF18002040
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.1 4.4 – 3.8 3.9 4.0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 4.5 4.6 – 5.9 5.8 5.5 0 0 0 0 0 0
Cytoplasm 0.4 1.3 3.6 3.1 2.5 – 3.9 5.3 3.9 1.3 0.8 0.5 3.5 2.7 2.2
Endoplasmic Reticulum 0 0 3.0 3.2 5.0 – 4.3 4.3 5.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 2.7 4.6 2.9 4.0 4.5 – 4.1 3.6 3.6 1.3 1.9 2.6 7.7 7.2 5.9
Mitochondria -0.4 2.7 5.1 3.5 3.9 – 3.9 6.9 8.2 -3.2 -3.2 -3.4 -1.5 -2.2 -1.5
Nucleus -2.3 -0.6 1.0 -0.9 -1.7 – -1.1 -1.4 -2.2 1.3 -1.3 -1.5 -0.3 -0.7 0.6
Nuclear Periphery 1.7 -6.8 -2.5 -0.5 -1.4 – -4.2 -4.2 -0.4 -4.6 -0.4 0.6 -3.3 -4.6 -4.5
Nucleolus -1.2 -3.7 -3.4 -4.7 -3.0 – -5.6 -5.4 -4.7 -2.3 -2.9 -2.9 -1.4 -1.8 -1.2
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 1.6 0.4 -6.9 -6.7 -9.4 – -7.4 -10.6 -12.3 2.3 2.2 2.1 0.1 1.5 0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.5265 27.8845 25.0397 23.6462 19.5937 25.4284 25.2756 33.1729 30.2104 27.284 24.2355 30.6466 26.9054 34.9446 31.8723 29.4898 28.0148 31.3896
Actin 0.0049 0 0.0004 0 0.0001 0 0.0103 0 0.0026 0.0132 0.0002 0 0.0017 0 0 0 0 0
Bud 0.0005 0.0001 0.0009 0.0001 0.0005 0 0.0002 0 0.0001 0.0001 0.0009 0.0001 0 0 0 0 0 0
Bud Neck 0.0002 0 0.0001 0 0.0001 0 0.0011 0 0.0002 0.0001 0.0002 0 0 0 0 0 0 0
Bud Periphery 0.0008 0.0005 0.0028 0.0004 0.0011 0.0002 0.0004 0 0.0001 0.0001 0.003 0.0004 0.0001 0 0.0001 0 0.0001 0
Bud Site 0.0007 0 0.0002 0 0.0001 0 0.0016 0 0.0004 0.0001 0.0028 0 0.0002 0 0 0 0 0
Cell Periphery 0.003 0.0017 0.0024 0.0018 0.0039 0.0016 0.0018 0.0008 0.002 0.0004 0.0019 0.0009 0.0013 0.0012 0.0015 0.0006 0.0013 0.0011
Cytoplasm 0.0016 0.0001 0.0004 0.0005 0.0043 0.0001 0.0022 0.0001 0.0015 0.0018 0.0021 0 0.0002 0 0 0 0 0
Cytoplasmic Foci 0.0034 0 0.0001 0.0002 0.0009 0.0001 0.0067 0 0.0027 0.0003 0.0004 0.0001 0.0012 0 0.0001 0 0 0
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0 0.0001 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0017 0.0001 0.0034 0.0001 0.0022 0.0001 0.0009 0.0001 0.0017 0.0146 0.0005 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001
Endosome 0.0156 0.0012 0.0037 0.0069 0.0524 0.003 0.0044 0.0008 0.0042 0.0082 0.0026 0.0009 0.0016 0.0006 0.0019 0.0006 0.0008 0.0004
Golgi 0.0027 0 0.0002 0.0002 0.0012 0.0001 0.0009 0 0.0018 0.0015 0.0002 0.0001 0.0002 0 0.0002 0 0 0
Lipid Particles 0.0046 0.0001 0.0003 0.0003 0.0002 0.0003 0.0082 0.0002 0.0037 0.0003 0.0013 0.0018 0.0016 0.0002 0.0006 0.0001 0.0005 0.0003
Mitochondria 0.0099 0.0059 0.0108 0.0012 0.0024 0.0002 0.0125 0.0002 0.006 0.001 0.0075 0.0085 0.0037 0.0004 0.0086 0.0001 0.0002 0.0005
None 0.0023 0 0.0001 0 0.0002 0 0.003 0 0.0003 0.0008 0.0035 0 0.0003 0 0 0 0 0
Nuclear Periphery 0.01 0.0057 0.0036 0.0038 0.0083 0.0069 0.0064 0.018 0.0138 0.0149 0.0219 0.0166 0.0045 0.0072 0.005 0.0004 0.0023 0.0046
Nucleolus 0.0066 0.001 0.001 0.0012 0.0013 0.0007 0.0024 0.0004 0.0089 0.0001 0.008 0.0045 0.0041 0.0008 0.0017 0.0001 0.0008 0.0003
Nucleus 0.0046 0.0033 0.0021 0.0041 0.003 0.0007 0.0023 0.0004 0.003 0.0016 0.0316 0.0007 0.0012 0.0004 0.0009 0.0002 0.0006 0.0003
Peroxisomes 0.0013 0 0 0 0 0 0.0005 0 0.0029 0 0.0002 0 0.001 0 0 0 0 0
Punctate Nuclear 0.0031 0 0 0 0.0001 0 0.0036 0 0.0008 0.0004 0.0033 0 0.0001 0 0 0 0 0
Vacuole 0.2843 0.3018 0.2974 0.3311 0.3659 0.2327 0.2047 0.1658 0.182 0.157 0.1481 0.1113 0.1925 0.1945 0.2158 0.2475 0.1815 0.0992
Vacuole Periphery 0.6382 0.6783 0.6703 0.648 0.552 0.7533 0.7258 0.8132 0.7615 0.7833 0.7596 0.854 0.7842 0.7946 0.7633 0.7502 0.8118 0.8931

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 254.0919 235.0905 233.4241 253.4613 209.9202 268.4493 230.5096 220.0935 215.469 247.1387
Translational Efficiency 1.0159 1.0562 1.0285 0.8528 1.0637 0.9309 1.0968 0.9529 1.0969 0.9858

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1717 2328 130 1182 1131 1528 1903 487 2848 3856 2033 1669

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1622.57 1906.22 3653.87 2552.26 2252.61 2090.29 2885.17 2699.67 1872.77 1979.16 2934.32 2595.27
Standard Deviation 476.05 681.18 659.02 842.01 605.67 529.02 599.92 731.41 614.28 631.78 632.48 814.06
Intensity Change Log 2 — 0.232434 1.171145 0.653495 — -0.107894 0.357059 0.261186 — 0.044477 0.754816 0.438584

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000040 0.000074 0.000173 0.000512 0.000008 0.000032 0.000048 0.000101 0.000027 0.000057 0.000056 0.000392
Bud Neck 0.000113 0.000281 0.001305 0.002549 0.000038 0.000178 0.000692 0.001295 0.000083 0.000240 0.000731 0.002183
Bud Site 0.001338 0.002374 0.000303 0.004750 0.000656 0.000419 0.000424 0.001251 0.001067 0.001600 0.000416 0.003729
Cell Periphery 0.002703 0.006373 0.000339 0.003356 0.007095 0.001424 0.002739 0.002011 0.004447 0.004412 0.002586 0.002963
Cytoplasm 0.001420 0.001958 0.000025 0.001126 0.000520 0.000151 0.000026 0.000502 0.001063 0.001242 0.000026 0.000944
Cytoplasmic Foci 0.000381 0.000588 0.002072 0.004793 0.000091 0.000254 0.000918 0.001860 0.000266 0.000456 0.000991 0.003938
Eisosomes 0.000011 0.000030 0.000079 0.000148 0.000009 0.000032 0.000255 0.000086 0.000010 0.000031 0.000244 0.000130
Endoplasmic Reticulum 0.000438 0.000756 0.000033 0.000793 0.000615 0.000174 0.000238 0.000157 0.000508 0.000525 0.000225 0.000607
Endosome 0.000743 0.000952 0.000193 0.003428 0.000262 0.000368 0.000205 0.001250 0.000552 0.000721 0.000204 0.002793
Golgi 0.002095 0.003083 0.001748 0.005511 0.001381 0.000622 0.000405 0.003355 0.001811 0.002108 0.000491 0.004882
Lipid Particles 0.000607 0.001040 0.000702 0.002319 0.000946 0.000732 0.002138 0.001133 0.000742 0.000918 0.002046 0.001973
Mitochondria 0.001337 0.001036 0.006826 0.010707 0.000544 0.001775 0.003032 0.008263 0.001022 0.001329 0.003274 0.009994
Mitotic Spindle 0.000011 0.000017 0.000117 0.003534 0.000011 0.000015 0.000013 0.000309 0.000011 0.000016 0.000019 0.002593
None 0.000618 0.001835 0.000358 0.000915 0.000518 0.000239 0.000355 0.000445 0.000578 0.001202 0.000355 0.000778
Nuclear Periphery 0.000187 0.000162 0.013036 0.001605 0.000227 0.000495 0.000629 0.000981 0.000203 0.000294 0.001423 0.001423
Nuclear Periphery Foci 0.000126 0.000182 0.004080 0.001193 0.000069 0.000251 0.000338 0.000626 0.000103 0.000209 0.000577 0.001027
Nucleolus 0.000919 0.002210 0.023483 0.010192 0.000531 0.002002 0.008089 0.004346 0.000765 0.002128 0.009074 0.008486
Nucleus 0.000355 0.001603 0.000634 0.002529 0.000214 0.000932 0.001466 0.001881 0.000299 0.001337 0.001413 0.002340
Peroxisomes 0.000057 0.000064 0.000275 0.000474 0.000019 0.000062 0.000123 0.000302 0.000042 0.000063 0.000133 0.000424
Vacuole 0.168348 0.256018 0.280964 0.324660 0.172112 0.236873 0.425472 0.319076 0.169843 0.248431 0.416232 0.323031
Vacuole Periphery 0.818153 0.719364 0.663254 0.614907 0.814138 0.752971 0.552394 0.650771 0.816558 0.732681 0.559483 0.625372

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.16 -2.85 -5.07 -4.72 -3.38 -4.20 -8.73 -3.69 -2.71 -2.10 -3.08 -3.48 -5.49 -5.05 -5.08
Bud Neck -3.14 -4.19 -6.78 -6.30 -2.98 -4.75 -9.35 -3.37 -3.02 -1.73 -4.58 -9.44 -7.58 -7.02 -5.28
Bud Site -1.57 2.06 -3.03 -2.24 -4.19 0.74 0.82 -1.47 -2.31 -2.48 -1.27 2.00 -3.35 -2.80 -4.37
Cell Periphery -6.69 6.20 -1.06 4.61 -5.87 6.89 4.73 5.33 -1.10 1.05 0.07 3.21 2.63 3.05 -0.68
Cytoplasm -0.69 2.30 0.19 0.90 -1.99 1.26 1.82 -0.02 -0.82 -1.12 -0.38 2.72 0.04 0.38 -2.23
Cytoplasmic Foci -1.78 -2.60 -4.51 -4.31 -2.53 -1.99 -4.32 -2.05 -1.85 -1.08 -2.45 -3.84 -4.96 -4.71 -3.96
Eisosomes -6.43 -3.51 -6.98 -5.93 -2.57 -4.21 -12.26 -3.16 -2.17 5.38 -7.39 -12.41 -7.66 -6.25 4.58
Endoplasmic Reticulum -4.17 6.78 -1.38 -0.16 -3.02 9.86 8.22 9.33 0.49 2.39 -0.35 6.49 -0.56 -0.47 -2.14
Endosome -1.00 3.14 -4.92 -4.61 -6.12 -0.88 1.05 -1.81 -1.59 -1.93 -1.25 3.41 -5.40 -5.03 -6.40
Golgi -1.80 0.57 -2.79 -1.95 -2.95 1.49 2.02 -1.58 -2.34 -2.54 -0.77 4.54 -3.27 -2.96 -4.86
Lipid Particles -7.42 -0.62 -3.72 -2.79 -3.36 2.90 -10.65 -0.96 -2.04 4.59 -3.85 -13.58 -3.72 -3.20 0.14
Mitochondria 1.13 -2.94 -5.62 -5.84 -1.60 -3.42 -4.19 -3.15 -2.64 -2.09 -1.44 -4.11 -6.50 -6.31 -4.61
Mitotic Spindle -2.07 -1.42 -3.21 -3.21 -3.11 -0.56 -0.34 -1.87 -1.84 -1.86 -1.61 -1.42 -3.32 -3.31 -3.31
None -3.22 1.38 -1.34 2.51 -3.80 0.78 0.46 0.17 -1.45 -0.67 -2.32 1.24 -1.00 1.80 -3.89
Nuclear Periphery 1.40 -2.68 -6.10 -6.22 2.38 -4.91 -4.35 -2.99 -1.95 -1.39 -3.71 -3.77 -6.76 -6.26 -0.04
Nuclear Periphery Foci -3.34 -3.13 -5.21 -4.94 2.24 -8.66 -9.59 -2.59 -1.84 -1.47 -8.07 -5.44 -5.86 -5.19 -2.60
Nucleolus -5.57 -2.74 -7.56 -6.45 1.58 -4.47 -11.05 -6.46 -3.50 4.11 -7.12 -9.97 -8.69 -7.05 0.39
Nucleus -3.88 -1.35 -5.60 -2.15 -4.50 -1.79 -9.44 -2.14 -1.09 -0.59 -4.12 -9.06 -5.77 -2.54 -2.75
Peroxisomes -0.29 -2.26 -2.67 -2.65 -1.14 -1.97 -10.08 -1.54 -1.29 -0.97 -1.25 -5.15 -3.11 -2.95 -2.39
Vacuole -14.32 -5.16 -19.24 -8.37 -1.95 -8.51 -33.60 -12.39 -6.68 8.64 -16.47 -38.03 -23.03 -10.95 11.47
Vacuole Periphery 14.55 6.65 21.94 11.23 2.09 7.57 32.95 12.83 7.83 -7.48 16.11 38.13 25.54 14.15 -7.26
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Subunit C of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane; the V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase) has eight subunits
Localization
Cell Percentages vacuolar membrane (90%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Vma5

Vma5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vma5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available