Standard name
Human Ortholog
Description Subunit C of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane; the V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase) has eight subunits

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.05 0.05 0.08 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.08 0.07 0.06 0.09 0.12 0.09 0.05 0 0 0.1 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.06 0.05 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.06 0.09 0.06 0.08 0.09 0.08 0.07 0.07 0 0.05 0.06 0.2 0.18 0.14 0 0 0 0 0 0
Mitochondria 0 0 0.05 0 0.1 0.15 0.11 0.12 0.12 0.2 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.1 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.06 0.05 0 0.08 0.09 0.07
Nuclear Periphery 0 0 0.16 0.21 0 0.11 0.15 0.13 0.09 0.08 0.15 0 0.15 0.18 0.06 0 0 0 0 0.11 0 0.05 0
Nucleolus 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.87 0.92 0.85 0.88 0.86 0.67 0.68 0.6 0.65 0.55 0.49 0.92 0.91 0.91 0.85 0.89 0.86 0.86 0.88 0.81 0.77 0.74 0.8
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 10 24 53 32 30 30 0 0 2 0 1 0 0 0 0 0 0 0
Bud 0 0 0 0 1 1 4 4 8 2 3 1 0 0 0 0 0 0 2 2 0 6 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1 4 7 13 56 62 78 68 60 0 0 1 1 0 1 0 0 0 0 0 0
Cytoplasm 13 6 12 17 25 72 77 71 81 99 72 10 8 8 17 16 15 1 0 1 3 2 1
Endoplasmic Reticulum 0 1 1 2 3 24 31 73 45 41 54 1 1 3 4 5 9 0 0 0 0 5 1
Endosome 0 0 1 1 2 0 1 1 3 1 0 0 0 0 1 0 0 1 1 4 1 2 3
Golgi 16 4 9 29 53 51 82 102 74 59 58 8 10 14 36 39 32 1 0 1 2 4 3
Mitochondria 4 0 21 23 55 131 117 136 110 163 193 0 0 0 4 3 6 2 1 11 5 7 3
Nucleus 36 7 14 6 15 40 27 22 21 17 12 11 3 3 5 5 10 23 7 7 14 36 23
Nuclear Periphery 8 2 68 105 21 101 163 156 79 70 125 7 30 41 11 8 10 9 0 52 6 19 15
Nucleolus 12 5 26 23 10 22 18 34 9 8 11 4 2 3 6 6 9 13 3 4 5 11 10
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 310 123 356 453 487 596 731 698 606 457 399 184 182 207 151 191 198 328 130 374 141 298 282
Unique Cell Count 356 134 419 512 567 893 1079 1167 927 834 816 201 199 228 177 214 230 383 148 462 184 402 352
Labelled Cell Count 399 148 509 664 679 1061 1331 1413 1146 1015 1017 226 236 282 236 274 290 383 148 462 184 402 352


Ambiguous, Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 36.8 34.5 24.4 25.3 23.4 21.3 20.9 20.3 19.2 21.1 19.4 29.2 31.9 28.6 36.2 36.9 37.6 33.0 33.5 36.1
Std Deviation (1e-4) 9.7 10.6 7.6 7.2 6.8 7.0 6.6 6.1 5.9 5.9 4.9 9.2 10.7 7.9 12.4 13.3 12.7 12.1 13.0 12.2
Intensity Change (Log2) 0.05 -0.06 -0.2 -0.22 -0.27 -0.35 -0.21 -0.34 0.26 0.38 0.23 0.57 0.6 0.62 0.44 0.46 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.1 4.4 3.8 3.9 4.0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 4.5 4.6 5.9 5.8 5.5 0 0 0 0 0 0
Cytoplasm 0.4 1.3 3.6 3.1 2.5 3.9 5.3 3.9 1.3 0.8 0.5 3.5 2.7 2.2
Endoplasmic Reticulum 0 0 3.0 3.2 5.0 4.3 4.3 5.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 2.7 4.6 2.9 4.0 4.5 4.1 3.6 3.6 1.3 1.9 2.6 7.7 7.2 5.9
Mitochondria -0.4 2.7 5.1 3.5 3.9 3.9 6.9 8.2 -3.2 -3.2 -3.4 -1.5 -2.2 -1.5
Nucleus -2.3 -0.6 1.0 -0.9 -1.7 -1.1 -1.4 -2.2 1.3 -1.3 -1.5 -0.3 -0.7 0.6
Nuclear Periphery 1.7 -6.8 -2.5 -0.5 -1.4 -4.2 -4.2 -0.4 -4.6 -0.4 0.6 -3.3 -4.6 -4.5
Nucleolus -1.2 -3.7 -3.4 -4.7 -3.0 -5.6 -5.4 -4.7 -2.3 -2.9 -2.9 -1.4 -1.8 -1.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.6 0.4 -6.9 -6.7 -9.4 -7.4 -10.6 -12.3 2.3 2.2 2.1 0.1 1.5 0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.5265 27.8845 25.0397 23.6462 19.5937 25.4284 25.2756 33.1729 30.2104 27.284 24.2355 30.6466 26.9054 34.9446 31.8723 29.4898 28.0148 31.3896
Actin 0.0049 0 0.0004 0 0.0001 0 0.0103 0 0.0026 0.0132 0.0002 0 0.0017 0 0 0 0 0
Bud 0.0005 0.0001 0.0009 0.0001 0.0005 0 0.0002 0 0.0001 0.0001 0.0009 0.0001 0 0 0 0 0 0
Bud Neck 0.0002 0 0.0001 0 0.0001 0 0.0011 0 0.0002 0.0001 0.0002 0 0 0 0 0 0 0
Bud Periphery 0.0008 0.0005 0.0028 0.0004 0.0011 0.0002 0.0004 0 0.0001 0.0001 0.003 0.0004 0.0001 0 0.0001 0 0.0001 0
Bud Site 0.0007 0 0.0002 0 0.0001 0 0.0016 0 0.0004 0.0001 0.0028 0 0.0002 0 0 0 0 0
Cell Periphery 0.003 0.0017 0.0024 0.0018 0.0039 0.0016 0.0018 0.0008 0.002 0.0004 0.0019 0.0009 0.0013 0.0012 0.0015 0.0006 0.0013 0.0011
Cytoplasm 0.0016 0.0001 0.0004 0.0005 0.0043 0.0001 0.0022 0.0001 0.0015 0.0018 0.0021 0 0.0002 0 0 0 0 0
Cytoplasmic Foci 0.0034 0 0.0001 0.0002 0.0009 0.0001 0.0067 0 0.0027 0.0003 0.0004 0.0001 0.0012 0 0.0001 0 0 0
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0 0.0001 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0017 0.0001 0.0034 0.0001 0.0022 0.0001 0.0009 0.0001 0.0017 0.0146 0.0005 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001
Endosome 0.0156 0.0012 0.0037 0.0069 0.0524 0.003 0.0044 0.0008 0.0042 0.0082 0.0026 0.0009 0.0016 0.0006 0.0019 0.0006 0.0008 0.0004
Golgi 0.0027 0 0.0002 0.0002 0.0012 0.0001 0.0009 0 0.0018 0.0015 0.0002 0.0001 0.0002 0 0.0002 0 0 0
Lipid Particles 0.0046 0.0001 0.0003 0.0003 0.0002 0.0003 0.0082 0.0002 0.0037 0.0003 0.0013 0.0018 0.0016 0.0002 0.0006 0.0001 0.0005 0.0003
Mitochondria 0.0099 0.0059 0.0108 0.0012 0.0024 0.0002 0.0125 0.0002 0.006 0.001 0.0075 0.0085 0.0037 0.0004 0.0086 0.0001 0.0002 0.0005
None 0.0023 0 0.0001 0 0.0002 0 0.003 0 0.0003 0.0008 0.0035 0 0.0003 0 0 0 0 0
Nuclear Periphery 0.01 0.0057 0.0036 0.0038 0.0083 0.0069 0.0064 0.018 0.0138 0.0149 0.0219 0.0166 0.0045 0.0072 0.005 0.0004 0.0023 0.0046
Nucleolus 0.0066 0.001 0.001 0.0012 0.0013 0.0007 0.0024 0.0004 0.0089 0.0001 0.008 0.0045 0.0041 0.0008 0.0017 0.0001 0.0008 0.0003
Nucleus 0.0046 0.0033 0.0021 0.0041 0.003 0.0007 0.0023 0.0004 0.003 0.0016 0.0316 0.0007 0.0012 0.0004 0.0009 0.0002 0.0006 0.0003
Peroxisomes 0.0013 0 0 0 0 0 0.0005 0 0.0029 0 0.0002 0 0.001 0 0 0 0 0
Punctate Nuclear 0.0031 0 0 0 0.0001 0 0.0036 0 0.0008 0.0004 0.0033 0 0.0001 0 0 0 0 0
Vacuole 0.2843 0.3018 0.2974 0.3311 0.3659 0.2327 0.2047 0.1658 0.182 0.157 0.1481 0.1113 0.1925 0.1945 0.2158 0.2475 0.1815 0.0992
Vacuole Periphery 0.6382 0.6783 0.6703 0.648 0.552 0.7533 0.7258 0.8132 0.7615 0.7833 0.7596 0.854 0.7842 0.7946 0.7633 0.7502 0.8118 0.8931

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 254.0919 235.0905 233.4241 253.4613 209.9202 268.4493 230.5096 220.0935 215.469 247.1387
Translational Efficiency 1.0159 1.0562 1.0285 0.8528 1.0637 0.9309 1.0968 0.9529 1.0969 0.9858

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit C of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane; the V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase) has eight subunits
Localization
Cell Percentages vacuolar membrane (90%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Vma5

Vma5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vma5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available