Standard name
Human Ortholog
Description Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.07 0.06 0.06 0.09 0.14 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 1.0 0.97 0.96 0.97 0.88 0.82 0.79 0.82 0.75 0.78 0.98 0.97 0.97 0.97 0.96 1.0 0.96 0.97 0.91 0.92 0.93 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0.1 0.14 0.13 0.1 0.13 0.09 0 0 0 0.06 0.07 0.09 0 0 0 0 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 5 2 17 11 7 12 0 0 0 0 0 0 0 0 0 0 0 1
Bud 1 4 0 0 0 0 1 1 0 0 2 2 0 0 0 0 0 0 3 3 1 1 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 4 2 1 4 6 20 19 15 26 25 26 2 1 2 4 10 4 0 0 0 0 0 0
Cytoplasm 600 472 242 192 250 243 258 247 213 249 133 215 251 290 328 512 320 255 563 481 217 150 198 187
Endoplasmic Reticulum 5 6 1 4 9 8 29 43 36 31 23 25 2 9 6 33 22 22 8 2 8 6 5 11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 3 2 0 0 4 5 4 5 4 0 0 2 10 4 0 0 0 2 0 3 5
Mitochondria 0 0 1 0 0 0 0 2 9 6 1 25 0 0 0 0 0 0 0 0 0 0 1 1
Nucleus 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 2 0 0 0 0 0 0 0 1
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 2 2 0 1 0 3 6 0 1 4 1 0 0 3
Unique Cell Count 607 480 243 198 261 251 294 302 271 302 178 276 255 300 337 529 332 255 587 498 240 163 214 223
Labelled Cell Count 609 490 246 200 265 257 313 318 295 327 199 313 255 301 338 564 362 281 587 498 240 163 214 223


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 134.2 153.2 132.9 135.4 119.8 84.9 73.5 60.5 53.8 47.4 46.1 40.1 161.5 149.1 136.2 116.9 137.5 137.9 132.3 121.8 113.0
Std Deviation (1e-4) 26.9 31.9 31.8 29.7 22.7 19.7 15.8 13.0 11.9 11.2 10.4 9.5 31.6 33.9 33.3 40.9 41.1 40.9 35.8 35.0 35.8
Intensity Change (Log2) 0.03 -0.15 -0.65 -0.85 -1.14 -1.31 -1.49 -1.53 -1.73 0.28 0.17 0.04 -0.18 0.05 0.05 -0.01 -0.13 -0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 4.0 0 0 3.3 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3.5 3.3 3.0 4.1 5.5 4.3 0 0 0 0 1.8 0
Cytoplasm -2.2 -2.8 -2.3 -5.4 -6.8 -7.5 -6.7 -8.1 -7.6 -1.3 -2.4 -2.1 -2.4 -2.6 1.0
Endoplasmic Reticulum 0 0 0 4.7 5.9 5.6 4.9 5.5 4.5 0 0 0 3.7 3.8 4.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 4.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 141.6779 197.4818 180.0105 178.2633 115.0663 148.3772 167.1036 229.316 194.3809 177.9171 175.6726 205.0407 157.0559 236.9239 209.9824 203.3693 148.4067 197.8512
Actin 0.0124 0.0004 0.0019 0.0012 0.0105 0.0007 0.0067 0.0003 0.0044 0.0036 0.0017 0.0011 0.0067 0.0004 0.0004 0.0003 0.1987 0.0034
Bud 0.0037 0.0012 0.0032 0.0026 0.0011 0.0055 0.0023 0.0009 0.0047 0.0028 0.0023 0.0018 0.0026 0.0008 0.0022 0.0015 0.0026 0.0019
Bud Neck 0.0036 0.0012 0.002 0.0011 0.0009 0.0017 0.0009 0.001 0.001 0.0008 0.001 0.0044 0.0009 0.0006 0.0006 0.0062 0.0009 0.0023
Bud Periphery 0.0053 0.0013 0.0027 0.0014 0.0011 0.003 0.0045 0.0007 0.0052 0.0032 0.0024 0.002 0.0019 0.0004 0.0008 0.0005 0.0029 0.0011
Bud Site 0.0085 0.0013 0.0047 0.0006 0.0015 0.0011 0.0016 0.0019 0.0013 0.0018 0.0005 0.0003 0.0027 0.0008 0.0012 0.0002 0.0031 0.0002
Cell Periphery 0.0743 0.0564 0.056 0.0414 0.009 0.0059 0.0495 0.0259 0.0192 0.0502 0.0086 0.0131 0.0524 0.018 0.0261 0.0022 0.001 0.0025
Cytoplasm 0.4143 0.7194 0.5572 0.3508 0.3307 0.6003 0.6582 0.881 0.7682 0.5704 0.7767 0.8039 0.5818 0.9213 0.8185 0.7888 0.614 0.9378
Cytoplasmic Foci 0.0123 0.0005 0.0075 0.0033 0.0962 0.0118 0.0101 0.0039 0.0125 0.0214 0.0113 0.0031 0.0783 0.0005 0.001 0.0023 0.011 0.0038
Eisosomes 0.0021 0 0.0002 0 0.0003 0.0001 0.0019 0.0007 0.0004 0.0001 0 0 0.0038 0 0 0 0.0005 0
Endoplasmic Reticulum 0.0276 0.0088 0.0044 0.003 0.0082 0.0026 0.0482 0.0051 0.014 0.0043 0.0067 0.0328 0.0116 0.0034 0.001 0.0013 0.0056 0.0054
Endosome 0.0054 0.0013 0.0034 0.0103 0.0072 0.0067 0.0091 0.0009 0.0136 0.0221 0.0308 0.0076 0.0036 0.0006 0.0018 0.0081 0.0165 0.0031
Golgi 0.0017 0.0001 0.0013 0.0048 0.0072 0.0004 0.0026 0.0001 0.0017 0.009 0.0014 0.0007 0.0027 0.0001 0.0002 0.0001 0.0692 0.0006
Lipid Particles 0.0071 0.001 0.0025 0.0007 0.0168 0.0008 0.005 0.0013 0.004 0.0059 0.0026 0.0009 0.0114 0.0006 0.0013 0.0001 0.0033 0.0006
Mitochondria 0.0233 0.0005 0.0095 0.0027 0.0259 0.0132 0.0073 0.001 0.0066 0.0286 0.001 0.005 0.0347 0.0002 0.0004 0.0002 0.0256 0.0017
None 0.0087 0.0002 0.0023 0.0006 0.0175 0.0175 0.01 0.0008 0.0053 0.0052 0.0074 0.0043 0.0328 0.0004 0.0006 0.0002 0.0009 0.001
Nuclear Periphery 0.0501 0.0112 0.0122 0.016 0.023 0.0084 0.0201 0.0034 0.0094 0.0147 0.0098 0.0174 0.0107 0.0048 0.0055 0.0019 0.0134 0.0066
Nucleolus 0.0155 0.0005 0.0076 0.0021 0.0782 0.0543 0.0071 0.0019 0.0033 0.0025 0.0009 0.0047 0.0418 0.0002 0.0013 0.0001 0.0005 0.0005
Nucleus 0.019 0.0053 0.0109 0.0129 0.0167 0.0281 0.0109 0.0033 0.0065 0.0114 0.0067 0.0199 0.0113 0.0029 0.0078 0.0033 0.0068 0.0057
Peroxisomes 0.0033 0 0.002 0.0002 0.0273 0.0006 0.0005 0.0001 0.0005 0.0006 0.0002 0.0001 0.0028 0 0.0001 0.0001 0.0056 0.0002
Punctate Nuclear 0.0044 0 0.0003 0.0001 0.027 0.001 0.0007 0.0001 0.0005 0.0007 0.0012 0.0002 0.0063 0 0.0001 0 0.0006 0.0003
Vacuole 0.2348 0.1666 0.2529 0.3976 0.1947 0.1671 0.1265 0.0623 0.1033 0.2058 0.1057 0.0609 0.0881 0.0418 0.1106 0.1386 0.0137 0.0157
Vacuole Periphery 0.0624 0.0225 0.0551 0.1468 0.0988 0.0692 0.0163 0.0034 0.0144 0.0347 0.0213 0.0159 0.0112 0.0022 0.0183 0.0441 0.0038 0.0058

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1762.8645 1951.2575 2140.8617 1740.5958 1816.2654 1656.4156 1673.4757 1381.8378 1461.4749 1255.9642
Translational Efficiency 1.1824 1.2211 1.1678 1.4613 1.2893 1.2056 1.2487 1.6007 1.2427 1.386

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Tef4

Tef4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tef4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available