Standard name | |
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Human Ortholog | |
Description | Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 5 | 2 | 17 | 11 | 7 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 1 | 4 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 1 | 1 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 4 | 2 | 1 | 4 | 6 | 20 | 19 | 15 | 26 | 25 | 26 | 2 | 1 | 2 | 4 | 10 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 600 | 472 | 242 | 192 | 250 | 243 | 258 | 247 | 213 | 249 | 133 | 215 | 251 | 290 | 328 | 512 | 320 | 255 | 563 | 481 | 217 | 150 | 198 | 187 |
Endoplasmic Reticulum | 5 | 6 | 1 | 4 | 9 | 8 | 29 | 43 | 36 | 31 | 23 | 25 | 2 | 9 | 6 | 33 | 22 | 22 | 8 | 2 | 8 | 6 | 5 | 11 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 3 | 2 | 0 | 0 | 4 | 5 | 4 | 5 | 4 | 0 | 0 | 2 | 10 | 4 | 0 | 0 | 0 | 2 | 0 | 3 | 5 |
Mitochondria | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 9 | 6 | 1 | 25 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 0 | 3 | 6 | 0 | 1 | 4 | 1 | 0 | 0 | 3 |
Unique Cell Count | 607 | 480 | 243 | 198 | 261 | 251 | 294 | 302 | 271 | 302 | 178 | 276 | 255 | 300 | 337 | 529 | 332 | 255 | 587 | 498 | 240 | 163 | 214 | 223 |
Labelled Cell Count | 609 | 490 | 246 | 200 | 265 | 257 | 313 | 318 | 295 | 327 | 199 | 313 | 255 | 301 | 338 | 564 | 362 | 281 | 587 | 498 | 240 | 163 | 214 | 223 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 134.2 | 153.2 | 132.9 | 135.4 | 119.8 | 84.9 | 73.5 | 60.5 | 53.8 | 47.4 | 46.1 | 40.1 | 161.5 | 149.1 | 136.2 | 116.9 | 137.5 | 137.9 | 132.3 | 121.8 | 113.0 |
Std Deviation (1e-4) | 26.9 | 31.9 | 31.8 | 29.7 | 22.7 | 19.7 | 15.8 | 13.0 | 11.9 | 11.2 | 10.4 | 9.5 | 31.6 | 33.9 | 33.3 | 40.9 | 41.1 | 40.9 | 35.8 | 35.0 | 35.8 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.15 | -0.65 | -0.85 | -1.14 | -1.31 | -1.49 | -1.53 | -1.73 | 0.28 | 0.17 | 0.04 | -0.18 | 0.05 | 0.05 | -0.01 | -0.13 | -0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 4.0 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 3.5 | 3.3 | 3.0 | 4.1 | 5.5 | 4.3 | 0 | 0 | 0 | 0 | 1.8 | 0 |
Cytoplasm | -2.2 | -2.8 | -2.3 | -5.4 | -6.8 | -7.5 | -6.7 | -8.1 | -7.6 | -1.3 | -2.4 | -2.1 | -2.4 | -2.6 | 1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 4.7 | 5.9 | 5.6 | 4.9 | 5.5 | 4.5 | 0 | 0 | 0 | 3.7 | 3.8 | 4.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 141.6779 | 197.4818 | 180.0105 | 178.2633 | 115.0663 | 148.3772 | 167.1036 | 229.316 | 194.3809 | 177.9171 | 175.6726 | 205.0407 | 157.0559 | 236.9239 | 209.9824 | 203.3693 | 148.4067 | 197.8512 |
Actin | 0.0124 | 0.0004 | 0.0019 | 0.0012 | 0.0105 | 0.0007 | 0.0067 | 0.0003 | 0.0044 | 0.0036 | 0.0017 | 0.0011 | 0.0067 | 0.0004 | 0.0004 | 0.0003 | 0.1987 | 0.0034 |
Bud | 0.0037 | 0.0012 | 0.0032 | 0.0026 | 0.0011 | 0.0055 | 0.0023 | 0.0009 | 0.0047 | 0.0028 | 0.0023 | 0.0018 | 0.0026 | 0.0008 | 0.0022 | 0.0015 | 0.0026 | 0.0019 |
Bud Neck | 0.0036 | 0.0012 | 0.002 | 0.0011 | 0.0009 | 0.0017 | 0.0009 | 0.001 | 0.001 | 0.0008 | 0.001 | 0.0044 | 0.0009 | 0.0006 | 0.0006 | 0.0062 | 0.0009 | 0.0023 |
Bud Periphery | 0.0053 | 0.0013 | 0.0027 | 0.0014 | 0.0011 | 0.003 | 0.0045 | 0.0007 | 0.0052 | 0.0032 | 0.0024 | 0.002 | 0.0019 | 0.0004 | 0.0008 | 0.0005 | 0.0029 | 0.0011 |
Bud Site | 0.0085 | 0.0013 | 0.0047 | 0.0006 | 0.0015 | 0.0011 | 0.0016 | 0.0019 | 0.0013 | 0.0018 | 0.0005 | 0.0003 | 0.0027 | 0.0008 | 0.0012 | 0.0002 | 0.0031 | 0.0002 |
Cell Periphery | 0.0743 | 0.0564 | 0.056 | 0.0414 | 0.009 | 0.0059 | 0.0495 | 0.0259 | 0.0192 | 0.0502 | 0.0086 | 0.0131 | 0.0524 | 0.018 | 0.0261 | 0.0022 | 0.001 | 0.0025 |
Cytoplasm | 0.4143 | 0.7194 | 0.5572 | 0.3508 | 0.3307 | 0.6003 | 0.6582 | 0.881 | 0.7682 | 0.5704 | 0.7767 | 0.8039 | 0.5818 | 0.9213 | 0.8185 | 0.7888 | 0.614 | 0.9378 |
Cytoplasmic Foci | 0.0123 | 0.0005 | 0.0075 | 0.0033 | 0.0962 | 0.0118 | 0.0101 | 0.0039 | 0.0125 | 0.0214 | 0.0113 | 0.0031 | 0.0783 | 0.0005 | 0.001 | 0.0023 | 0.011 | 0.0038 |
Eisosomes | 0.0021 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0019 | 0.0007 | 0.0004 | 0.0001 | 0 | 0 | 0.0038 | 0 | 0 | 0 | 0.0005 | 0 |
Endoplasmic Reticulum | 0.0276 | 0.0088 | 0.0044 | 0.003 | 0.0082 | 0.0026 | 0.0482 | 0.0051 | 0.014 | 0.0043 | 0.0067 | 0.0328 | 0.0116 | 0.0034 | 0.001 | 0.0013 | 0.0056 | 0.0054 |
Endosome | 0.0054 | 0.0013 | 0.0034 | 0.0103 | 0.0072 | 0.0067 | 0.0091 | 0.0009 | 0.0136 | 0.0221 | 0.0308 | 0.0076 | 0.0036 | 0.0006 | 0.0018 | 0.0081 | 0.0165 | 0.0031 |
Golgi | 0.0017 | 0.0001 | 0.0013 | 0.0048 | 0.0072 | 0.0004 | 0.0026 | 0.0001 | 0.0017 | 0.009 | 0.0014 | 0.0007 | 0.0027 | 0.0001 | 0.0002 | 0.0001 | 0.0692 | 0.0006 |
Lipid Particles | 0.0071 | 0.001 | 0.0025 | 0.0007 | 0.0168 | 0.0008 | 0.005 | 0.0013 | 0.004 | 0.0059 | 0.0026 | 0.0009 | 0.0114 | 0.0006 | 0.0013 | 0.0001 | 0.0033 | 0.0006 |
Mitochondria | 0.0233 | 0.0005 | 0.0095 | 0.0027 | 0.0259 | 0.0132 | 0.0073 | 0.001 | 0.0066 | 0.0286 | 0.001 | 0.005 | 0.0347 | 0.0002 | 0.0004 | 0.0002 | 0.0256 | 0.0017 |
None | 0.0087 | 0.0002 | 0.0023 | 0.0006 | 0.0175 | 0.0175 | 0.01 | 0.0008 | 0.0053 | 0.0052 | 0.0074 | 0.0043 | 0.0328 | 0.0004 | 0.0006 | 0.0002 | 0.0009 | 0.001 |
Nuclear Periphery | 0.0501 | 0.0112 | 0.0122 | 0.016 | 0.023 | 0.0084 | 0.0201 | 0.0034 | 0.0094 | 0.0147 | 0.0098 | 0.0174 | 0.0107 | 0.0048 | 0.0055 | 0.0019 | 0.0134 | 0.0066 |
Nucleolus | 0.0155 | 0.0005 | 0.0076 | 0.0021 | 0.0782 | 0.0543 | 0.0071 | 0.0019 | 0.0033 | 0.0025 | 0.0009 | 0.0047 | 0.0418 | 0.0002 | 0.0013 | 0.0001 | 0.0005 | 0.0005 |
Nucleus | 0.019 | 0.0053 | 0.0109 | 0.0129 | 0.0167 | 0.0281 | 0.0109 | 0.0033 | 0.0065 | 0.0114 | 0.0067 | 0.0199 | 0.0113 | 0.0029 | 0.0078 | 0.0033 | 0.0068 | 0.0057 |
Peroxisomes | 0.0033 | 0 | 0.002 | 0.0002 | 0.0273 | 0.0006 | 0.0005 | 0.0001 | 0.0005 | 0.0006 | 0.0002 | 0.0001 | 0.0028 | 0 | 0.0001 | 0.0001 | 0.0056 | 0.0002 |
Punctate Nuclear | 0.0044 | 0 | 0.0003 | 0.0001 | 0.027 | 0.001 | 0.0007 | 0.0001 | 0.0005 | 0.0007 | 0.0012 | 0.0002 | 0.0063 | 0 | 0.0001 | 0 | 0.0006 | 0.0003 |
Vacuole | 0.2348 | 0.1666 | 0.2529 | 0.3976 | 0.1947 | 0.1671 | 0.1265 | 0.0623 | 0.1033 | 0.2058 | 0.1057 | 0.0609 | 0.0881 | 0.0418 | 0.1106 | 0.1386 | 0.0137 | 0.0157 |
Vacuole Periphery | 0.0624 | 0.0225 | 0.0551 | 0.1468 | 0.0988 | 0.0692 | 0.0163 | 0.0034 | 0.0144 | 0.0347 | 0.0213 | 0.0159 | 0.0112 | 0.0022 | 0.0183 | 0.0441 | 0.0038 | 0.0058 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1762.8645 | 1951.2575 | 2140.8617 | 1740.5958 | 1816.2654 | 1656.4156 | 1673.4757 | 1381.8378 | 1461.4749 | 1255.9642 |
Translational Efficiency | 1.1824 | 1.2211 | 1.1678 | 1.4613 | 1.2893 | 1.2056 | 1.2487 | 1.6007 | 1.2427 | 1.386 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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