Standard name
Human Ortholog
Description Centromeric CDEIII region binding protein; nucleates kinetochore assembly; required for the structural integrity of elongating spindles; copurifies with subunits of the MIND complex and centromeric nucleosome components (Cse4p and histones H2A, H2B, and H4); phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3; localizes to the kinetochore
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.05 0 0 0 0 0 0 0.08 0.09 0.06 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0.05 0.11 0.05 0 0 0 0 0.05 0.09 0.05 0.05 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0
Bud Site 0.13 0 0 0.05 0 0.07 0.08 0.05 0.09 0.1 0.1 0.1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.18 0.1 0.13 0.13 0.1 0.09 0.12 0.07 0.08 0.1 0.05 0.19 0.31 0.33 0.18 0.2 0.18 0 0 0 0.06 0.12 0.15
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.16 0.08 0.14 0.15 0.07 0.06 0 0 0 0 0 0.17 0.27 0.3 0.13 0.15 0.2 0.08 0.13 0.11 0.1 0.07 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.23 0.11 0.08 0.09 0.12 0.19 0.31 0.3 0.41 0.4 0.34 0.49 0 0 0 0.21 0 0.07 0.05 0.05 0.05 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.05 0.06 0.06 0.13 0.15 0.11 0.11 0.12 0.17 0.18 0.12 0.07 0.07 0 0.05 0.08 0.13 0 0.05 0 0 0 0
Peroxisomes 0.08 0.13 0.05 0.09 0.09 0.11 0.11 0 0.08 0.06 0.08 0 0 0.11 0.16 0 0 0 0 0 0 0 0 0
SpindlePole 0.62 0.66 0.74 0.73 0.68 0.67 0.69 0.56 0.57 0.54 0.52 0.5 0.67 0.5 0.49 0.74 0.7 0.73 0.74 0.59 0.72 0.69 0.63 0.51
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 0 0 0 0 3 0 0 0 1 1 1 2 1 3 4 5
Bud 6 1 4 7 5 7 11 23 25 18 7 9 1 0 0 7 3 6 1 0 2 2 4 9
Bud Neck 7 4 13 9 1 6 6 13 25 15 5 5 12 7 3 34 14 12 2 2 4 1 5 10
Bud Site 17 1 7 11 3 18 26 14 25 28 10 13 8 6 1 6 4 7 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 13 7 26 26 18 26 29 34 19 23 10 7 48 52 69 124 70 50 4 4 6 12 32 37
Endoplasmic Reticulum 0 0 0 1 1 0 0 0 1 0 1 0 0 0 1 21 13 7 0 0 0 1 1 2
Endosome 2 6 20 29 21 17 19 12 10 5 0 2 44 45 61 87 52 55 10 14 33 19 19 18
Golgi 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 2 0 2 2 1 1
Mitochondria 31 4 19 18 17 49 98 85 118 112 33 63 3 1 1 142 12 18 6 5 14 6 10 12
Nucleus 1 0 1 1 0 4 4 7 7 8 1 4 0 0 2 9 8 6 0 0 1 0 1 1
Nuclear Periphery 0 0 0 0 1 1 1 1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 15 2 15 12 18 39 36 31 33 47 17 15 18 12 6 32 28 35 1 5 4 3 2 1
Peroxisomes 11 5 13 19 13 29 36 8 23 18 8 5 8 19 32 18 3 4 1 1 4 1 1 1
SpindlePole 82 25 185 149 95 173 219 160 162 151 50 64 172 84 100 508 246 198 108 68 211 138 176 128
Vac/Vac Membrane 1 0 2 0 1 0 1 3 3 0 0 1 4 2 4 9 4 5 1 5 2 2 8 9
Unique Cell Count 133 38 250 204 140 259 319 285 285 278 97 129 255 168 206 688 351 272 146 116 295 201 279 251
Labelled Cell Count 186 55 306 283 195 369 487 391 451 425 142 188 322 228 280 998 460 405 146 116 295 201 279 251


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.6 4.0 3.8 4.0 3.5 3.0 3.5 3.2 3.1 3.3 2.9 4.5 4.8 4.9 5.0 6.0 5.7 4.0 4.2 5.2
Std Deviation (1e-4) 0.9 1.8 1.2 1.4 1.5 1.1 1.0 1.5 1.1 1.6 1.7 1.2 1.1 1.9 2.1 1.3 1.7 1.6 1.6 1.7 2.1
Intensity Change (Log2) – – – -0.05 -0.0 -0.19 -0.4 -0.17 -0.31 -0.35 -0.26 -0.43 0.17 0.27 0.3 0.33 0.59 0.53 0.03 0.09 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0002 0.0029 0.0008 0.0002 0.0003 0.0005 0 0.0004 0.0485 0.0003 0.0613 0.0356 0.0009 0.0008 0.0002 0.0043 0.0795 0.0005
Bud 0.0008 0.0039 0.0005 0 0 0.0001 0.0007 0.0002 0.0009 0.0001 0.0002 0.0001 0 0.0002 0 0.0004 0.0006 0
Bud Neck 0.0009 0.0018 0.0009 0.0233 0.0003 0.0044 0.0002 0.0009 0.0015 0.0009 0.0003 0.0033 0.0002 0.0022 0.0005 0.0037 0.0012 0.0025
Bud Periphery 0.0008 0.0027 0.0007 0 0 0.0002 0.0004 0.0001 0.0013 0.0001 0.0002 0.0001 0 0.0002 0 0.0005 0.0008 0
Bud Site 0.0104 0.019 0.0108 0.0001 0.0003 0.0004 0.0032 0.0043 0.0045 0.0015 0.0005 0.0012 0.0002 0.0119 0.0002 0.0113 0.0047 0.0002
Cell Periphery 0.0009 0.0007 0.0004 0 0.0001 0.0001 0.0011 0.0001 0.0001 0 0 0 0 0.0001 0 0.0004 0.0002 0
Cytoplasm 0.0205 0.0253 0.0024 0.0031 0.0216 0.0263 0.1337 0.1506 0.0008 0.0121 0.0005 0.0029 0.0049 0.0386 0.0024 0.0019 0.0048 0.004
Cytoplasmic Foci 0.011 0.0494 0.0302 0.099 0.2343 0.0528 0.0044 0.1103 0.1333 0.2092 0.1101 0.0929 0.0913 0.0575 0.0296 0.244 0.0923 0.0341
Eisosomes 0.0001 0.0002 0.0006 0 0 0.0001 0 0.0001 0.0005 0 0.0001 0.0001 0 0.0001 0 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.0029 0.0022 0.0007 0.0001 0.0033 0.0015 0.0047 0.0041 0.0003 0.0004 0.0002 0.0001 0.0001 0.0016 0.0001 0.0007 0.0015 0.0001
Endosome 0.0346 0.0123 0.0146 0.0072 0.1094 0.0073 0.0689 0.0177 0.0089 0.0045 0.0094 0.0116 0.0041 0.0084 0.0007 0.0285 0.0598 0.0014
Golgi 0.001 0.0034 0.0027 0.002 0.0032 0.0009 0.0003 0.0022 0.0322 0.0013 0.0203 0.0027 0.0027 0.0054 0.0002 0.0102 0.0218 0.0005
Lipid Particles 0.0025 0.0045 0.0048 0.0345 0.0331 0.0148 0.0013 0.008 0.017 0.0055 0.0415 0.0117 0.0138 0.003 0.0017 0.0448 0.1036 0.0046
Mitochondria 0.001 0.0048 0.005 0.0001 0.0009 0.0254 0.0008 0.0004 0.0941 0.0003 0.0012 0.0002 0.0004 0.001 0.0002 0.0016 0.0089 0.0001
None 0.2546 0.1861 0.0462 0.239 0.2302 0.0404 0.0148 0.2702 0.0014 0.0217 0.0006 0.0043 0.005 0.164 0.0187 0.0082 0.0049 0.0701
Nuclear Periphery 0.0112 0.0047 0.0009 0.0014 0.0021 0.0059 0.04 0.0134 0.0005 0.005 0.0005 0.0007 0.0012 0.0265 0.002 0.001 0.005 0.0023
Nucleolus 0.0162 0.014 0.007 0.0017 0.0007 0.027 0.0077 0.0125 0.0091 0.0115 0.0009 0.0107 0.0041 0.0169 0.0192 0.0024 0.0043 0.0166
Nucleus 0.0737 0.0479 0.0047 0.0022 0.0008 0.0429 0.127 0.0284 0.0039 0.0633 0.0002 0.0028 0.0212 0.1065 0.0451 0.0011 0.0021 0.0431
Peroxisomes 0.0004 0.0018 0.0012 0.0058 0.0045 0.0034 0.0001 0.0016 0.1119 0.0059 0.1849 0.0198 0.0199 0.0112 0.0005 0.031 0.1383 0.0012
Punctate Nuclear 0.4969 0.6026 0.8604 0.5801 0.3422 0.7439 0.0012 0.372 0.5289 0.6552 0.5669 0.7988 0.8298 0.5396 0.8786 0.599 0.4605 0.8181
Vacuole 0.0547 0.0087 0.0037 0.0001 0.0112 0.0015 0.5373 0.0023 0.0002 0.0008 0 0.0004 0.0001 0.0019 0.0001 0.0043 0.004 0.0002
Vacuole Periphery 0.0047 0.0012 0.0009 0.0001 0.0013 0.0003 0.0523 0.0005 0.0003 0.0002 0.0001 0 0 0.0024 0 0.0004 0.001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.034 7.1736 6.2103 8.351 13.8726 16.0406 6.6488 5.3034 7.4532 13.759
Translational Efficiency 2.8847 1.8678 1.0052 1.3947 1.692 1.94 1.5347 1.2841 1.2926 1.4247

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
85 1462 998 1666 2116 1709 184 1669 2201 3171 1182 3335

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 654.64 706.76 748.10 793.44 633.57 686.23 869.54 781.27 634.38 695.70 767.00 787.35
Standard Deviation 125.55 92.29 103.42 99.21 74.28 96.19 85.28 105.96 77.01 94.96 110.00 102.83
Intensity Change Log 2 — 0.110519 0.192529 0.277419 — 0.115188 0.456748 0.302317 — 0.112817 0.328523 0.289718

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.016895 0.000344 0.000371 0.001063 0.000216 0.000307 0.001146 0.001121 0.000861 0.000324 0.000491 0.001092
Bud Neck 0.066764 0.015093 0.008365 0.005581 0.012559 0.019137 0.006618 0.006508 0.014652 0.017273 0.008093 0.006045
Bud Site 0.033625 0.006833 0.020549 0.037794 0.004208 0.008618 0.014451 0.029612 0.005344 0.007795 0.019600 0.033699
Cell Periphery 0.000394 0.000078 0.000296 0.000090 0.000153 0.000092 0.000262 0.000154 0.000163 0.000086 0.000290 0.000122
Cytoplasm 0.159731* 0.080422 0.250320* 0.064694 0.148717* 0.126279* 0.105691* 0.141277* 0.149143* 0.105136* 0.227806* 0.103020*
Cytoplasmic Foci 0.268767* 0.194005* 0.037463 0.010336 0.380451* 0.292236* 0.022681 0.023799 0.376138* 0.246946* 0.035162 0.017074
Eisosomes 0.000259 0.000064 0.000097 0.000020 0.000113 0.000072 0.000214 0.000038 0.000119 0.000069 0.000116 0.000029
Endoplasmic Reticulum 0.001568 0.000557 0.001680 0.001349 0.000720 0.000517 0.006725 0.000783 0.000753 0.000535 0.002465 0.001066
Endosome 0.024923 0.030433 0.006454 0.005495 0.019055 0.032827 0.009088 0.010057 0.019282 0.031723 0.006864 0.007778
Golgi 0.017406 0.013240 0.000447 0.002957 0.012890 0.016532 0.000645 0.004161 0.013065 0.015014 0.000478 0.003559
Lipid Particles 0.018224 0.043526 0.004668 0.001094 0.072259 0.040483 0.007705 0.002972 0.070172 0.041886 0.005141 0.002034
Mitochondria 0.011339 0.010876 0.001577 0.002514 0.011720 0.018643 0.001000 0.002262 0.011705 0.015062 0.001488 0.002388
Mitotic Spindle 0.120417* 0.435309* 0.071266 0.773682* 0.137268* 0.271438* 0.214505* 0.638744* 0.136617* 0.346991* 0.093564 0.706152*
None 0.040348 0.009152 0.026228 0.003649 0.023876 0.012296 0.007117 0.007592 0.024513 0.010846 0.023253 0.005622
Nuclear Periphery 0.000337 0.001802 0.001390 0.000963 0.001338 0.000957 0.001731 0.002037 0.001299 0.001346 0.001443 0.001501
Nuclear Periphery Foci 0.005729 0.019006 0.005894 0.004377 0.016257 0.014314 0.028897 0.004792 0.015850 0.016478 0.009475 0.004585
Nucleolus 0.023985 0.040994 0.037801 0.004896 0.050360 0.039849 0.026609 0.011993 0.049341 0.040377 0.036059 0.008448
Nucleus 0.102611 0.053829 0.502503* 0.056174 0.060004 0.053715 0.459695* 0.081542 0.061650 0.053767 0.495839* 0.068869
Peroxisomes 0.022990 0.037839 0.002622 0.003851 0.039588 0.042915 0.003638 0.006318 0.038947 0.040574 0.002781 0.005086
Vacuole 0.061564 0.005185 0.019371 0.017704 0.007078 0.007612 0.080982 0.022768 0.009183 0.006493 0.028962 0.020238
Vacuole Periphery 0.002122 0.001411 0.000638 0.001717 0.001168 0.001161 0.000599 0.001469 0.001204 0.001277 0.000632 0.001593

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.01 0.95 0.94 -7.30 -0.67 -3.61 -9.15 -6.40 -5.70 4.24 0.73 -1.58 -1.34 -8.87 0.76
Bud Neck 1.98 1.73 1.79 -2.48 0.72 -2.84 -1.27 -3.97 -0.15 -1.84 -1.12 -3.27 -3.05 -1.81 0.86
Bud Site 2.80 1.40 -0.38 -9.48 -4.25 -3.69 -6.55 -10.71 -9.40 -1.13 -2.06 -6.30 -14.06 -13.33 -4.73
Cell Periphery 2.45 0.80 2.12 -4.39 8.94 4.42 -5.97 -1.34 -4.69 5.30 6.05 -8.62 0.71 -6.31 9.52
Cytoplasm 2.78 -0.46 2.48 -1.36 9.01 0.18 1.33 -2.75 -2.74 -2.87 2.59 -6.09 0.07 -2.78 6.40
Cytoplasmic Foci 2.53 7.73 8.38 30.07 5.45 7.14 37.22 50.09 38.10 0.45 12.93 46.78 52.99 48.18 4.51
Eisosomes 3.56 0.97 3.71 1.18 11.04 3.70 -8.52 3.61 0.52 9.28 5.49 -9.71 5.94 1.29 13.80
Endoplasmic Reticulum 1.69 -5.51 -3.79 -13.38 3.16 -0.11 -10.58 -11.97 -12.56 8.32 -0.56 -15.94 -16.95 -18.36 7.57
Endosome -0.79 2.64 3.38 13.51 3.79 -6.95 -3.21 5.93 11.30 5.45 -8.55 3.33 9.10 17.59 4.69
Golgi 0.38 1.99 2.00 10.25 0.35 -2.21 13.06 12.01 11.34 0.64 -1.19 15.04 13.90 15.36 -0.65
Lipid Particles -3.09 3.51 4.16 14.06 5.86 5.69 17.02 19.78 15.11 5.31 6.16 18.80 20.09 20.66 7.40
Mitochondria 0.49 1.74 1.89 5.05 2.48 -2.05 6.69 6.01 5.62 -2.74 -1.51 5.92 6.36 7.40 2.19
Mitotic Spindle -6.79 -0.51 -14.83 -20.51 -36.26* -10.75 -5.09 -37.56* -25.06* -10.14 -17.93 -1.70 -50.80 -32.51* -37.95*
None 2.25 1.28 2.64 5.37 10.55 3.68 5.99 10.75 6.37 1.09 5.79 -0.16 11.64 8.33 10.40
Nuclear Periphery -2.97 -9.52 -10.71 -4.63 -2.28 3.25 -5.88 -3.77 -5.45 2.85 1.00 -8.33 -7.38 -7.39 -0.04
Nuclear Periphery Foci -1.40 0.18 0.69 5.63 1.48 2.56 -4.96 7.57 4.60 7.00 1.88 1.54 8.31 7.22 5.22
Nucleolus -1.56 -6.08 1.91 12.18 18.17 6.25 3.16 14.33 7.74 3.52 6.07 -5.70 17.74 14.08 17.60
Nucleus 1.85 -12.14 -0.51 -8.57 22.12 0.01 -8.40 -10.41 -9.96 5.92 -0.09 -28.82 -13.05 -13.14 23.28
Peroxisomes -2.85 5.49 5.65 16.30 0.62 -3.87 13.44 18.03 19.38 0.71 -2.99 21.47 21.53 25.47 -0.69
Vacuole 2.62 1.50 0.44 -12.18 -4.82 -2.04 -7.14 -12.01 -11.58 4.26 0.82 -9.65 -15.48 -16.79 -1.67
Vacuole Periphery 1.25 1.58 1.55 0.58 0.11 -0.54 -0.67 -0.72 -0.39 -0.24 -0.58 -0.26 -0.35 0.31 -0.22
Standard name
Human Ortholog
Description Centromeric CDEIII region binding protein; nucleates kinetochore assembly; required for the structural integrity of elongating spindles; copurifies with subunits of the MIND complex and centromeric nucleosome components (Cse4p and histones H2A, H2B, and H4); phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3; localizes to the kinetochore
Localization
Cell Percentages spindle pole (32%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mif2

Mif2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mif2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available