Standard name
Human Ortholog
Description GTPase activating factor for Rsr1p/Bud1p; plays a role in spindle position checkpoint distinct from its role in bud site selection; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; contains two PH-like domains
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.11 0.07 0.1 0.11 0.13 0.15 0.09 0.1 0.07 – 0.06 0.05 0.16 0.34 0.28 0.19 0.21 0.12 0.09 0.05 0.09 0.2 0.21 0.19
Bud 0.09 0.05 0.09 0.14 0.17 0.17 0.13 0.16 0.16 – 0.2 0.18 0.08 0.16 0.16 0.12 0.15 0.05 0.05 0 0.05 0.15 0.21 0.3
Bud Neck 0 0 0.05 0.07 0.14 0.09 0.1 0.08 0.07 – 0.1 0.09 0.06 0.07 0.09 0 0.08 0 0.25 0.2 0.19 0.4 0.42 0.4
Bud Site 0 0 0 0.09 0.1 0.12 0.07 0.07 0.09 – 0.1 0.14 0 0.07 0.13 0.06 0 0 – – – – – –
Cell Periphery 0.23 0.18 0.17 0.2 0.05 0.08 0.08 0.05 0.1 – 0.06 0.07 0.14 0.1 0.1 0.12 0.13 0.07 0.11 0.09 0.16 0.06 0 0
Cytoplasm 0.79 0.87 0.81 0.61 0.44 0.4 0.39 0.37 0.32 – 0.24 0.2 0.73 0.53 0.47 0.56 0.41 0.55 0.42 0.55 0.39 0.12 0.06 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.12 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.1 0.06 0.19 0.22 0.3 0.39 – 0.48 0.47 0 0 0 0.06 0 0.05 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0.07 0.07 0.07 0 – 0.06 0.1 0 0 0.07 0.06 0 0.05 0 0 0 0 0 0
SpindlePole 0 0 0 0.06 0.14 0.08 0.15 0.14 0.08 – 0.1 0.13 0.06 0.06 0.07 0.12 0.26 0.14 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 11 10 31 24 36 45 32 36 19 – 20 17 36 76 68 3 8 7 9 8 30 37 61 56
Bud 9 7 30 31 48 50 46 57 42 – 66 63 18 36 39 2 6 3 5 5 17 27 61 86
Bud Neck 3 1 16 16 39 27 35 30 18 – 32 30 14 15 23 0 3 0 26 32 63 74 121 113
Bud Site 1 0 3 19 28 35 24 27 24 – 32 49 4 15 31 1 1 2 – – – – – –
Cell Periphery 22 27 55 45 14 25 27 19 26 – 21 26 32 22 24 2 5 4 11 15 53 10 6 5
Cytoplasm 77 128 262 136 123 120 140 137 83 – 78 72 166 118 116 9 16 32 44 91 132 23 17 7
Endoplasmic Reticulum 3 2 6 3 7 8 11 9 3 – 3 5 2 3 2 2 2 4 2 2 7 0 2 2
Endosome 1 1 0 2 5 2 2 4 0 – 0 0 5 4 6 0 1 4 0 1 7 1 3 2
Golgi 1 0 2 0 1 0 0 0 0 – 0 0 0 0 0 0 1 0 2 0 2 0 0 0
Mitochondria 0 4 6 23 17 58 80 110 101 – 159 164 1 1 5 1 1 3 1 5 16 6 5 7
Nucleus 0 0 0 1 5 3 13 7 6 – 8 12 0 0 0 0 0 4 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 1 0 1 1 0 – 0 1 0 1 1 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 3 8 6 9 12 – 8 8 0 1 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 1 4 9 20 26 25 10 – 20 35 0 7 17 1 0 3 0 0 0 0 1 0
SpindlePole 2 0 12 14 38 24 54 53 21 – 32 45 13 14 16 2 10 8 0 0 3 0 1 1
Vac/Vac Membrane 0 0 0 0 1 0 4 2 1 – 0 2 0 0 0 0 0 2 0 2 3 1 2 1
Unique Cell Count 97 147 324 222 277 302 358 367 257 329 352 227 224 246 16 39 58 108 167 342 186 290 288
Labelled Cell Count 130 181 424 318 375 425 501 526 366 479 529 291 313 348 23 55 76 108 167 342 186 290 288


Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 5.9 5.0 4.9 5.2 4.8 4.7 4.6 4.2 – 4.0 3.8 5.6 5.7 5.0 7.4 8.1 8.3 5.9 5.8 6.4
Std Deviation (1e-4) 0.7 1.0 1.1 1.5 1.9 1.3 1.4 1.5 1.7 – 1.3 1.1 0.9 0.7 0.9 0.9 1.9 2.3 1.6 1.3 1.3
Intensity Change (Log2) – – – -0.02 0.06 -0.05 -0.08 -0.11 -0.26 – -0.31 -0.38 0.17 0.19 0.0 0.58 0.7 0.74 0.25 0.22 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.5 1.3 0 0 0 0 – 0 0 2.2 7.1 5.6 0 0 0.6
Bud 1.7 2.9 0 0 0 0 – 0 0 -0.5 2.4 2.4 0 0 -1.0
Bud Neck 1.1 3.9 0 0 0 0 – 0 0 0.6 0.9 2.1 0 0 0
Bud Site 4.5 5.1 0 0 0 0 – 0 0 0 3.7 5.8 0 0 0
Cell Periphery 1.0 -4.6 0 0 0 0 – 0 0 -0.9 -2.4 -2.5 0 -0.7 -2.0
Cytoplasm -5.1 -9.3 -10.5 -11.1 -11.6 -11.8 – -14.6 -15.7 -2.1 -7.0 -8.4 -2.4 -5.6 -4.3
Endoplasmic Reticulum 0 0.6 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 4.4 2.7 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 3.5 0 0 – 0 3.4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 4.5 0 0 0
SpindlePole 1.4 4.4 0 0 0 0 – 0 0 1.1 1.4 1.5 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1092 3.2823 3.4387 3.1495 3.2644 3.3444 4.2301 4.7654 4.7304 5.0942 4.3769 4.508 4.3181 5.2113 4.688 4.7056 4.0988 4.788
Actin 0.0605 0.0059 0.0352 0.0104 0.0221 0.0267 0.019 0.0091 0.04 0.0065 0.0486 0.0096 0.0253 0.0131 0.0307 0.0037 0.0163 0.0107
Bud 0.0039 0.0025 0.0025 0.0018 0.0028 0.0033 0.008 0.0094 0.0073 0.0117 0.007 0.0062 0.0014 0.0031 0.0027 0.0007 0.0008 0.0009
Bud Neck 0.0277 0.014 0.4354 0.4908 0.3005 0.124 0.0199 0.0072 0.3595 0.5121 0.2117 0.0912 0.04 0.002 0.3684 0.6535 0.2655 0.1308
Bud Periphery 0.0076 0.0032 0.0031 0.0044 0.0067 0.0107 0.0187 0.0078 0.0096 0.0285 0.0147 0.0158 0.004 0.0051 0.0068 0.001 0.0013 0.0037
Bud Site 0.1575 0.183 0.1314 0.0145 0.0097 0.0143 0.1509 0.1746 0.1856 0.0322 0.0286 0.0074 0.1671 0.1156 0.1516 0.0484 0.0194 0.0033
Cell Periphery 0.0306 0.0159 0.0065 0.0057 0.0044 0.0098 0.0229 0.0138 0.013 0.0071 0.006 0.0072 0.0515 0.0131 0.0139 0.0021 0.0037 0.0092
Cytoplasm 0.399 0.6348 0.2591 0.3526 0.5092 0.6696 0.5083 0.6476 0.2565 0.231 0.4869 0.685 0.5836 0.7243 0.3381 0.2739 0.6236 0.7171
Cytoplasmic Foci 0.0207 0.0097 0.0279 0.0057 0.0073 0.0145 0.02 0.007 0.0244 0.0339 0.0134 0.0124 0.0103 0.0019 0.0115 0.0055 0.0251 0.0048
Eisosomes 0.0017 0.0002 0.0005 0.0001 0.0003 0.0006 0.0004 0.0003 0.0005 0.0004 0.0003 0.0001 0.0008 0.0003 0.0008 0.0001 0.0003 0.0003
Endoplasmic Reticulum 0.0097 0.0041 0.003 0.005 0.0035 0.0026 0.0073 0.002 0.0016 0.0014 0.0023 0.0024 0.0067 0.0072 0.0018 0.0018 0.0022 0.0033
Endosome 0.044 0.0039 0.0079 0.0353 0.0083 0.0082 0.0146 0.0013 0.0115 0.0464 0.0243 0.0048 0.0152 0.0005 0.0073 0.0014 0.0034 0.0007
Golgi 0.0179 0.0022 0.0047 0.0044 0.0073 0.0037 0.0025 0.0008 0.006 0.0061 0.0156 0.0051 0.0022 0.0004 0.0013 0.001 0.0028 0.0004
Lipid Particles 0.0356 0.0008 0.0047 0.012 0.0094 0.0043 0.0102 0.0007 0.005 0.0127 0.0158 0.0031 0.0046 0.0004 0.0031 0.0001 0.0014 0.0007
Mitochondria 0.0306 0.0002 0.0025 0.0112 0.0479 0.0016 0.0026 0.0004 0.0032 0.0033 0.0105 0.0046 0.0014 0.0001 0.0004 0.0002 0.0002 0.0001
None 0.0741 0.1163 0.0329 0.0267 0.0395 0.0793 0.1488 0.1097 0.0574 0.0438 0.0713 0.1299 0.0742 0.1108 0.05 0.0048 0.028 0.1118
Nuclear Periphery 0.024 0.0003 0.0079 0.0015 0.0004 0.0036 0.0132 0.0006 0.0007 0.0009 0.0011 0.0014 0.0014 0.0004 0.0012 0.0001 0.0003 0.0002
Nucleolus 0.0064 0.0002 0.0008 0.0007 0.0003 0.0023 0.0015 0.0006 0.0007 0.0027 0.0025 0.0003 0.0002 0.0001 0.0011 0.0001 0.0006 0.0001
Nucleus 0.0114 0.0009 0.0058 0.0024 0.0009 0.0026 0.0127 0.0038 0.0032 0.0015 0.0023 0.002 0.0009 0.0006 0.0018 0.0006 0.0011 0.0005
Peroxisomes 0.0178 0.0002 0.0221 0.0017 0.013 0.0089 0.0032 0.0003 0.0097 0.0035 0.0178 0.0078 0.0032 0.0001 0.0018 0.0001 0.0009 0.0002
Punctate Nuclear 0.0072 0.0003 0.0032 0.0005 0.0003 0.0059 0.0034 0.0012 0.0024 0.002 0.011 0.0009 0.0017 0.0002 0.0021 0.0002 0.0023 0.0003
Vacuole 0.0086 0.0014 0.0023 0.0101 0.0055 0.0028 0.0103 0.0019 0.0019 0.0102 0.0068 0.0026 0.004 0.0007 0.0033 0.0007 0.001 0.0011
Vacuole Periphery 0.0033 0.0001 0.0007 0.0027 0.0007 0.0006 0.0017 0.0001 0.0002 0.002 0.0016 0.0004 0.0005 0 0.0003 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.644 10.1167 5.5199 12.7689 15.7518 9.6474 12.0341 9.1375 10.7583 20.1735
Translational Efficiency 1.0099 0.7517 0.8923 0.5451 0.6568 1.3355 0.6196 0.4871 0.5853 0.5434

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
616 223 155 1468 1761 2275 171 180 2377 2498 326 1648

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 707.31 828.98 1143.94 885.87 730.79 806.89 1019.25 919.28 724.71 808.86 1078.54 889.52
Standard Deviation 76.59 103.77 119.47 113.73 96.45 104.71 102.38 109.75 92.30 104.82 127.13 113.78
Intensity Change Log 2 — 0.228995 0.693597 0.324752 — 0.142915 0.479979 0.331047 — 0.185894 0.588996 0.327955

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002200 0.003938 0.007474 0.008307 0.004299 0.005365 0.002536 0.025412 0.003755 0.005237 0.004884 0.010175
Bud Neck 0.218454* 0.103035 0.044672 0.103957 0.180811* 0.197681* 0.112519 0.104997 0.190566* 0.189232* 0.080260 0.104071
Bud Site 0.077590 0.119996* 0.203289* 0.246170* 0.122554* 0.149211* 0.170487* 0.361500* 0.110901* 0.146603* 0.186083* 0.258767*
Cell Periphery 0.007060 0.001442 0.001100 0.000582 0.003617 0.000956 0.001023 0.000795 0.004510 0.000999 0.001060 0.000605
Cytoplasm 0.478929* 0.366436* 0.219905* 0.330318* 0.414476* 0.395621* 0.202803* 0.182227* 0.431179* 0.393016* 0.210934* 0.314143*
Cytoplasmic Foci 0.078275 0.197585* 0.054046 0.029202 0.128622 0.103564 0.057824 0.033944 0.115574 0.111957 0.056028 0.029720
Eisosomes 0.009841 0.002926 0.000269 0.000186 0.005196 0.001752 0.000302 0.000350 0.006400 0.001857 0.000286 0.000204
Endoplasmic Reticulum 0.003409 0.000824 0.010793 0.000598 0.001823 0.000895 0.002630 0.001206 0.002234 0.000889 0.006511 0.000664
Endosome 0.012179 0.044565 0.014275 0.013335 0.017946 0.018738 0.015699 0.007019 0.016452 0.021044 0.015022 0.012645
Golgi 0.007882 0.010775 0.000538 0.003731 0.010358 0.010581 0.000406 0.002917 0.009716 0.010599 0.000469 0.003642
Lipid Particles 0.008407 0.006891 0.006096 0.001049 0.006718 0.003210 0.003845 0.001020 0.007156 0.003539 0.004915 0.001046
Mitochondria 0.002633 0.004775 0.000134 0.005400 0.005834 0.007063 0.000124 0.001329 0.005004 0.006858 0.000129 0.004955
Mitotic Spindle 0.000390 0.000334 0.000734 0.035951 0.000337 0.004102 0.002541 0.009879 0.000350 0.003765 0.001682 0.033103
None 0.001333 0.000094 0.000325 0.000672 0.001545 0.000616 0.000585 0.000155 0.001490 0.000569 0.000462 0.000615
Nuclear Periphery 0.000194 0.000063 0.000793 0.000228 0.000080 0.000084 0.000455 0.000135 0.000109 0.000082 0.000616 0.000218
Nuclear Periphery Foci 0.000410 0.000755 0.014316 0.000540 0.000309 0.000377 0.005465 0.001001 0.000335 0.000410 0.009674 0.000590
Nucleolus 0.000258 0.000209 0.000227 0.000311 0.000587 0.000284 0.000263 0.000088 0.000502 0.000278 0.000246 0.000287
Nucleus 0.033020 0.011315 0.084545 0.022547 0.036465 0.020795 0.059876 0.010368 0.035572 0.019948 0.071605 0.021217
Peroxisomes 0.004854 0.018691 0.010742 0.021929 0.008828 0.012219 0.014022 0.022549 0.007798 0.012797 0.012462 0.021997
Vacuole 0.051643 0.103259 0.325445* 0.171766 0.048488 0.065625 0.346374* 0.228150* 0.049306 0.068985 0.336423* 0.177925
Vacuole Periphery 0.001041 0.002092 0.000281 0.003221 0.001108 0.001260 0.000219 0.004959 0.001090 0.001334 0.000248 0.003410

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.37 -1.90 -3.59 -1.61 0.19 -1.48 2.71 -2.55 -2.36 -2.90 -2.32 -0.42 -3.22 -1.71 -2.02
Bud Neck 6.81 14.11 12.02 2.69 -5.13 -1.96 6.66 9.42 11.22 0.35 0.21 13.78 18.13 18.74 -1.44
Bud Site -3.39 -2.65 -10.05 -3.54 -1.39 -4.44 0.06 -5.82 -4.68 -4.75 -6.76 -1.18 -8.60 -3.64 -3.16
Cell Periphery 8.96 11.05 12.26 3.06 5.86 6.72 6.87 7.46 2.91 1.58 10.66 10.88 12.53 7.21 6.22
Cytoplasm 5.68 12.87 9.26 0.86 -7.32 2.01 11.49 11.09 10.24 0.15 4.61 16.59 10.27 6.28 -8.91
Cytoplasmic Foci -9.58 4.57 10.73 14.25 4.62 5.24 12.68 16.13 12.66 2.32 0.87 14.57 25.45 24.49 6.02
Eisosomes 3.56 10.11 10.21 1.59 4.10 7.70 11.64 11.49 9.15 -0.79 10.13 15.33 15.55 8.14 5.00
Endoplasmic Reticulum 2.18 -5.14 2.23 0.16 9.54 1.90 -2.49 1.13 -1.05 4.01 2.66 -6.73 3.37 0.11 10.10
Endosome -5.44 -0.70 2.10 6.04 2.82 -0.50 2.28 5.82 6.66 3.55 -3.25 2.17 5.81 8.72 3.80
Golgi -1.51 12.73 7.02 4.66 -3.07 -0.25 18.23 5.21 5.25 -1.05 -1.17 21.44 11.03 10.59 -3.34
Lipid Particles 0.71 2.18 8.85 2.71 8.81 5.00 3.77 7.10 3.01 4.18 5.86 3.45 11.17 5.29 9.78
Mitochondria -0.81 6.66 -0.68 0.52 -3.53 -1.07 7.47 5.29 6.15 -1.42 -1.86 8.48 2.00 3.37 -3.64
Mitotic Spindle 0.19 -1.13 -8.72 -8.78 -8.63 -4.89 -1.01 -2.38 -1.62 -1.46 -4.85 -1.12 -9.06 -7.59 -7.11
None 3.97 3.26 1.49 -7.45 -4.89 3.37 2.92 4.96 2.70 1.04 4.03 4.45 3.21 -1.53 -2.29
Nuclear Periphery 1.41 -6.63 -2.56 -9.78 6.28 -0.09 -8.26 -5.28 -3.32 5.11 0.62 -11.91 -7.58 -6.98 5.99
Nuclear Periphery Foci -1.92 -9.63 -2.53 0.74 9.47 -0.38 -7.92 -3.42 -3.04 5.33 -0.50 -11.67 -4.61 -1.99 11.15
Nucleolus 0.94 1.09 -0.11 -0.62 -0.56 1.89 2.32 3.05 2.58 2.97 1.82 2.43 1.91 0.31 -0.33
Nucleus 8.79 -6.75 -2.61 -12.89 5.94 8.82 -3.51 9.83 3.28 6.03 10.24 -7.03 -1.01 -9.02 6.56
Peroxisomes -5.62 -1.54 -5.30 0.99 -2.74 -3.28 -0.54 -2.22 -1.28 -1.14 -5.57 -0.76 -4.24 -1.52 -2.19
Vacuole -6.18 -16.63 -28.74 -15.62 5.91 -6.34 -15.69 -12.09 -11.39 2.27 -7.95 -22.77 -32.47 -29.26 7.55
Vacuole Periphery -1.30 3.22 -1.34 0.21 -2.83 -0.85 7.96 -0.67 -0.62 -0.96 -1.36 8.19 -1.49 -1.08 -2.94
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description GTPase activating factor for Rsr1p/Bud1p; plays a role in spindle position checkpoint distinct from its role in bud site selection; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; contains two PH-like domains
Localization
Cell Percentages cytoplasm (45%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Bud2

Bud2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available