Standard name
Human Ortholog
Description Nim1p-related protein kinase; septin-binding kinase that localizes to the bud neck septin ring and regulates the morphogenesis checkpoint; phosphorylates Hsl7p and cooperates with Elm1p to recruit Hsl7p to the mother-bud neck, as a prerequisite for the subsequent recruitment, phosphorylation, and degradation of Swe1p; autophosphorylation enhances interactions with Hsl7p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.08 0 0.11 0.19 0.17 0.17 0.11 0.14 0.13 – 0.1 0.11 0.07 0.05 0.08 0.06 0.06 0.06 0.17 0.2 0.12 0.05 0 0
Bud Neck 0.16 0.11 0.16 0.21 0.25 0.17 0.17 0.16 0.15 – 0.15 0.16 0.16 0.24 0.22 0.17 0.19 0.12 0.32 0.3 0.32 0.1 0 0
Bud Site 0.06 0.09 0.13 0.09 0.13 0.1 0.08 0.05 0 – 0 0 0.12 0.11 0.18 0.08 0 0.05 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.62 0.64 0.58 0.58 0.39 0.49 0.55 0.57 0.61 – 0.55 0.55 0.54 0.42 0.38 0.56 0.55 0.63 0.33 0.31 0.3 0.76 0.85 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.09 0.14 0.11 0.11 0.19 0.15 0.17 0.27 – 0.36 0.32 0 0 0 0.06 0.05 0 0 0 0.08 0 0 0.06
Nucleus 0.06 0 0.07 0.06 0.07 0.05 0.06 0.07 0.06 – 0.08 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.07 0 0 0.06 0 0 0 0 – 0 0 0.08 0.06 0.09 0.05 0.07 0.06 0 0 0 0 0 0
SpindlePole 0.14 0 0.08 0.1 0.23 0.13 0.17 0.14 0.07 – 0.07 0.08 0.12 0.16 0.15 0.15 0.19 0.16 0.06 0 0.06 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 3 7 4 5 4 4 5 0 – 0 0 1 3 6 5 1 7 7 4 5 1 4 9
Bud 21 7 25 65 74 66 76 93 59 – 27 46 17 17 34 24 23 28 47 47 28 16 10 15
Bud Neck 41 23 35 72 111 65 114 106 67 – 39 69 39 89 93 65 74 57 92 71 72 29 5 3
Bud Site 15 18 29 31 58 36 50 33 19 – 11 18 30 42 76 29 13 25 – – – – – –
Cell Periphery 5 4 2 1 1 4 0 1 0 – 0 1 1 0 2 1 5 7 1 0 1 1 4 6
Cytoplasm 160 129 128 198 172 185 367 382 275 – 141 231 134 157 162 209 211 292 94 74 69 229 403 446
Endoplasmic Reticulum 0 0 2 0 0 0 0 1 1 – 0 2 0 0 1 24 10 28 2 1 1 1 0 4
Endosome 2 2 1 0 1 0 0 1 0 – 0 0 2 5 3 8 10 6 2 3 1 1 3 5
Golgi 1 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 2 2 0 0 1 2
Mitochondria 6 18 31 38 47 73 100 113 122 – 94 135 3 5 18 21 18 17 8 10 19 7 14 36
Nucleus 16 5 15 19 33 19 41 48 25 – 21 18 11 18 23 15 15 13 0 1 1 0 0 0
Nuclear Periphery 0 1 0 0 2 0 1 1 0 – 1 0 0 3 4 0 1 2 1 0 0 0 0 0
Nucleolus 2 5 1 6 4 6 12 11 11 – 4 15 4 13 30 6 5 4 1 0 0 1 0 0
Peroxisomes 6 15 3 7 25 7 11 8 4 – 3 2 20 23 38 18 26 27 2 0 2 0 0 0
SpindlePole 36 9 18 34 103 49 111 96 32 – 18 33 30 59 64 55 72 73 17 8 13 4 7 9
Vac/Vac Membrane 0 0 0 1 5 0 1 0 0 – 0 2 0 3 5 5 7 5 2 8 5 3 6 6
Unique Cell Count 258 202 221 342 440 378 663 667 452 258 420 249 372 426 375 382 463 286 240 228 300 475 557
Labelled Cell Count 315 239 297 476 641 514 888 899 616 359 572 292 437 559 485 491 591 286 240 228 300 475 557


Bud Neck

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 4.3 3.4 3.2 3.6 3.1 3.0 3.1 2.6 – 2.6 2.4 4.7 5.3 4.7 5.2 5.8 5.7 4.1 3.5 4.2
Std Deviation (1e-4) 1.0 1.4 1.0 1.2 1.5 1.0 1.3 1.2 1.2 – 0.9 1.1 1.4 1.7 1.5 1.3 1.6 1.6 1.0 0.9 1.3
Intensity Change (Log2) – – – -0.05 0.12 -0.11 -0.18 -0.13 -0.36 – -0.38 -0.46 0.5 0.66 0.49 0.63 0.78 0.76 0.28 0.07 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2206 3.8612 4.1523 3.7815 3.6847 3.3494 0.9583 1.6599 2.413 2.5373 1.9082 1.3991 0.377 1.2327 1.8548 2.1578 1.0546 1.1858
Actin 0.0575 0.0031 0.0036 0.0005 0.0336 0.0187 0.0242 0.0012 0.026 0.0574 0.0024 0.0065 0.0151 0.0009 0.0028 0.0001 0 0.0018
Bud 0.0167 0.0081 0.0072 0.0013 0.0008 0.006 0.0075 0.0137 0.0035 0.0025 0.0008 0.0052 0.0317 0.0114 0.0023 0.0001 0.0001 0.0054
Bud Neck 0.0842 0.0572 0.7037 0.8845 0.8509 0.464 0.046 0.0564 0.6914 0.6287 0.7449 0.3238 0.1298 0.049 0.6942 0.9944 0.9976 0.3213
Bud Periphery 0.0208 0.0095 0.0068 0.0011 0.0011 0.0098 0.0084 0.0075 0.0041 0.0029 0.0008 0.0082 0.0456 0.0153 0.003 0.0003 0.0001 0.0042
Bud Site 0.4409 0.5453 0.2144 0.0167 0.0151 0.0079 0.1451 0.4229 0.2022 0.0302 0.0112 0.0129 0.2543 0.5731 0.2299 0.0029 0.0005 0.0066
Cell Periphery 0.0041 0.0055 0.0036 0.0007 0.0005 0.0016 0.0009 0.0034 0.0014 0.0011 0.0003 0.0011 0.0022 0.0033 0.0014 0.0002 0.0001 0.0006
Cytoplasm 0.0243 0.041 0.0014 0.0002 0.0009 0.1421 0.0662 0.1243 0.0007 0.0047 0.0008 0.248 0.0164 0.0233 0.0008 0.0001 0.0001 0.1264
Cytoplasmic Foci 0.045 0.011 0.0072 0.0179 0.0251 0.0175 0.1014 0.0076 0.0285 0.0385 0.072 0.0692 0.0614 0.0035 0.0042 0.0001 0.0006 0.0342
Eisosomes 0.0017 0.0003 0.0006 0 0.0007 0.0001 0.0004 0.0001 0.0003 0.0003 0 0.0001 0.0004 0.0001 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0038 0.0015 0.0001 0 0.0021 0.0017 0.0041 0.0008 0.0009 0.0028 0.0002 0.001 0.002 0.0005 0.0001 0 0 0.0004
Endosome 0.0061 0.0004 0.0022 0.0001 0.0094 0.0027 0.0371 0.0031 0.0044 0.0358 0.0314 0.0026 0.0277 0.0002 0.0043 0 0 0.0004
Golgi 0.0044 0.0004 0.0013 0.0005 0.0055 0.0008 0.0054 0.0004 0.0075 0.0188 0.0462 0.0014 0.0099 0.0001 0.0011 0 0 0.0005
Lipid Particles 0.0175 0.002 0.0044 0.0023 0.0117 0.0012 0.0232 0.001 0.0081 0.0189 0.0039 0.0019 0.0203 0.0002 0.0019 0 0 0.0014
Mitochondria 0.0038 0.0006 0.0037 0 0.0046 0.0005 0.0023 0.0004 0.0038 0.0237 0.0152 0.0008 0.0026 0.0005 0.0059 0 0 0.0004
None 0.2257 0.2499 0.0141 0.0001 0.0032 0.3033 0.3871 0.3082 0.0005 0.0716 0.0002 0.2854 0.3287 0.2952 0.0352 0 0 0.4766
Nuclear Periphery 0.0049 0.0015 0.0005 0 0.0077 0.0012 0.0387 0.0004 0.0013 0.003 0.0001 0.0006 0.0026 0.0001 0 0 0 0.0002
Nucleolus 0.0071 0.0331 0.0052 0.0109 0.0031 0.0024 0.0071 0.0178 0.0014 0.0054 0.0157 0.0016 0.005 0.0031 0.0017 0.0008 0.0003 0.0008
Nucleus 0.0031 0.004 0.0018 0.002 0.0021 0.0027 0.0394 0.0195 0.0008 0.0028 0.0021 0.0026 0.0031 0.0028 0.0011 0.0004 0.0003 0.0012
Peroxisomes 0.014 0.0035 0.0062 0.0279 0.0099 0.0013 0.0077 0.0008 0.0096 0.0341 0.0259 0.0037 0.0188 0.0006 0.0034 0 0 0.0127
Punctate Nuclear 0.0083 0.0162 0.0102 0.0328 0.0106 0.0062 0.0404 0.0028 0.002 0.0042 0.0252 0.0193 0.0153 0.0151 0.0008 0.0002 0.0003 0.0044
Vacuole 0.0041 0.0057 0.0017 0.0003 0.0007 0.0077 0.0049 0.007 0.0011 0.0112 0.0004 0.0038 0.0061 0.0017 0.0056 0.0001 0 0.0004
Vacuole Periphery 0.002 0.0002 0.0002 0 0.0008 0.0006 0.0026 0.0005 0.0005 0.0014 0.0004 0.0004 0.001 0.0001 0.0003 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 82.0834 30.3034 11.0826 21.7754 97.4775 55.418 27.7399 19.5719 18.0811 97.243
Translational Efficiency 0.9644 0.6543 0.3801 0.2509 0.5908 1.0524 0.5218 0.4305 0.3559 0.5857

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1220 1280 2191 1297 1455 1623 1432 1214 2675 2903 3623 2511

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 611.28 659.42 923.49 931.81 675.85 698.47 887.64 912.15 646.40 681.25 909.32 922.30
Standard Deviation 93.11 96.02 123.20 118.20 84.25 93.79 103.39 516.87 94.07 96.74 117.10 369.43
Intensity Change Log 2 — 0.109364 0.595263 0.608202 — 0.047495 0.393272 0.432568 — 0.077209 0.492732 0.518650

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000182 0.000636 0.000366 0.002929 0.000208 0.000465 0.000384 0.001498 0.000196 0.000540 0.000373 0.002237
Bud Neck 0.257549* 0.249875* 0.204892* 0.167625 0.374906* 0.362063* 0.304396* 0.204675* 0.321382* 0.312597* 0.244221* 0.185538*
Bud Site 0.244327* 0.243129* 0.251220* 0.610527* 0.234939* 0.222058* 0.376076* 0.588298* 0.239221* 0.231349* 0.300570* 0.599780*
Cell Periphery 0.005158 0.001868 0.000704 0.001003 0.004715 0.002786 0.001992 0.001062 0.004917 0.002381 0.001213 0.001031
Cytoplasm 0.255800* 0.204022* 0.119504* 0.112098* 0.204638* 0.184844* 0.155121* 0.112872* 0.227972* 0.193300* 0.133582* 0.112472*
Cytoplasmic Foci 0.055300 0.101324 0.002530 0.012778 0.073307 0.085214 0.010280 0.010413 0.065094 0.092317 0.005593 0.011634
Eisosomes 0.000581 0.000181 0.000093 0.000194 0.000244 0.000213 0.000338 0.000245 0.000398 0.000199 0.000190 0.000218
Endoplasmic Reticulum 0.000792 0.000851 0.002364 0.000499 0.000343 0.000814 0.000651 0.000412 0.000547 0.000830 0.001687 0.000457
Endosome 0.011043 0.034948 0.001440 0.004425 0.015156 0.025595 0.004021 0.002952 0.013280 0.029719 0.002460 0.003713
Golgi 0.001757 0.008075 0.000337 0.002661 0.003327 0.005189 0.001128 0.001941 0.002611 0.006462 0.000650 0.002313
Lipid Particles 0.004726 0.004972 0.000322 0.000276 0.003203 0.004261 0.000491 0.000329 0.003897 0.004574 0.000389 0.000302
Mitochondria 0.002569 0.008971 0.001060 0.003508 0.004188 0.011200 0.000579 0.000979 0.003450 0.010218 0.000870 0.002285
Mitotic Spindle 0.003315 0.010923 0.009962 0.019043 0.003996 0.005868 0.008205 0.011713 0.003685 0.008097 0.009267 0.015499
None 0.010639 0.007484 0.003263 0.000297 0.003346 0.003319 0.001511 0.000573 0.006673 0.005155 0.002570 0.000430
Nuclear Periphery 0.000602 0.000638 0.001170 0.000113 0.000126 0.000196 0.000279 0.000104 0.000343 0.000391 0.000818 0.000109
Nuclear Periphery Foci 0.001394 0.001378 0.000324 0.000537 0.000766 0.000626 0.000876 0.000809 0.001052 0.000958 0.000542 0.000669
Nucleolus 0.005420 0.004576 0.001846 0.000120 0.001880 0.002086 0.000555 0.000175 0.003495 0.003184 0.001336 0.000147
Nucleus 0.109421 0.042035 0.373502* 0.009975 0.038036 0.035582 0.084445 0.010141 0.070593 0.038427 0.259251* 0.010055
Peroxisomes 0.007990 0.026590 0.001094 0.004300 0.009097 0.014411 0.004941 0.005030 0.008592 0.019781 0.002615 0.004653
Vacuole 0.020023 0.045473 0.023703 0.044838 0.022920 0.031166 0.043436 0.045125 0.021599 0.037474 0.031502 0.044976
Vacuole Periphery 0.001412 0.002050 0.000306 0.002254 0.000660 0.002044 0.000294 0.000654 0.001003 0.002046 0.000302 0.001480

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.17 -6.99 -3.47 -2.87 -3.10 -2.89 -5.14 -4.53 -3.63 -3.68 -4.29 -8.64 -4.85 -4.03 -4.22
Bud Neck 0.89 9.05 10.98 10.79 3.06 1.08 8.49 16.89 16.46 8.89 1.18 14.42 20.27 20.06 7.41
Bud Site 0.38 5.14 -21.26 -22.60 -28.82* 1.46 -10.01 -25.04 -26.94* -15.08 1.33 -1.52 -32.76 -35.14* -32.52*
Cell Periphery 4.92 7.17 6.64 3.48 -3.36 2.57 4.03 5.50 5.09 3.65 4.97 8.02 8.43 6.17 1.34
Cytoplasm 4.35 14.36 10.08 5.76 -3.61 1.93 3.10 7.03 5.40 4.01 4.44 12.81 11.85 7.72 0.00
Cytoplasmic Foci -8.74 19.70 14.14 18.18 -10.32 -2.17 16.25 16.02 17.26 -0.40 -7.12 24.63 20.94 24.87 -8.77
Eisosomes 5.24 6.57 5.13 -0.50 -7.49 1.31 -2.18 0.30 -0.99 2.44 5.34 5.74 4.91 -0.91 -1.98
Endoplasmic Reticulum -0.34 -13.46 -0.99 -0.60 13.43 -2.19 -7.73 -3.92 0.50 2.70 -1.97 -16.14 -3.18 0.02 13.67
Endosome -10.78 10.02 6.10 14.66 -3.50 -4.99 9.47 10.44 12.47 1.86 -10.79 14.19 11.70 18.90 -2.12
Golgi -7.66 10.44 -0.72 6.00 -3.46 -3.37 7.17 2.44 4.69 -1.16 -8.08 11.00 1.53 7.57 -3.43
Lipid Particles -0.42 7.44 7.37 7.63 -0.51 -1.55 6.26 6.62 6.46 1.82 -1.38 9.81 9.90 9.82 0.79
Mitochondria -4.90 1.85 -0.45 4.51 -2.38 -4.02 4.06 3.59 6.74 -1.24 -5.98 4.22 2.17 7.85 -2.46
Mitotic Spindle -4.27 -4.98 -6.27 -3.39 -2.80 -1.46 -3.51 -4.55 -3.66 -2.05 -4.13 -6.08 -7.74 -5.14 -3.26
None 1.84 5.04 7.27 6.61 17.86 -0.01 3.20 4.99 4.74 3.14 1.71 5.50 8.68 8.17 13.43
Nuclear Periphery -0.15 -6.06 1.65 1.36 21.29 -1.18 -9.45 -2.94 -0.29 6.18 -0.36 -9.51 1.04 1.19 21.73
Nuclear Periphery Foci -0.09 3.07 1.66 1.87 -2.35 0.82 -1.03 -0.69 -1.80 0.36 0.38 2.28 0.96 0.60 -1.84
Nucleolus 0.91 3.64 5.88 5.98 5.59 -0.30 3.54 4.54 6.17 6.95 0.74 4.21 7.27 8.19 6.29
Nucleus 10.48 -34.64 13.77 4.48 50.29 0.55 -11.69 5.43 4.85 14.68 8.95 -36.42 14.08 6.41 46.62
Peroxisomes -7.56 6.19 3.41 10.31 -5.65 -3.21 3.76 3.29 7.01 -0.74 -7.84 7.64 4.82 12.51 -4.71
Vacuole -8.99 -7.51 -14.11 -9.31 -11.01 -3.81 -10.48 -11.23 -9.78 -3.99 -9.19 -12.51 -17.94 -13.77 -11.23
Vacuole Periphery -1.52 2.89 -0.58 0.23 -2.25 -4.99 5.85 0.32 4.35 -1.52 -4.29 3.93 -0.62 1.94 -2.56
Standard name
Human Ortholog
Description Nim1p-related protein kinase; septin-binding kinase that localizes to the bud neck septin ring and regulates the morphogenesis checkpoint; phosphorylates Hsl7p and cooperates with Elm1p to recruit Hsl7p to the mother-bud neck, as a prerequisite for the subsequent recruitment, phosphorylation, and degradation of Swe1p; autophosphorylation enhances interactions with Hsl7p
Localization
Cell Percentages bud neck (31%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Hsl1

Hsl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hsl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available