Standard name | |
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Human Ortholog | |
Description | Nim1p-related protein kinase; septin-binding kinase that localizes to the bud neck septin ring and regulates the morphogenesis checkpoint; phosphorylates Hsl7p and cooperates with Elm1p to recruit Hsl7p to the mother-bud neck, as a prerequisite for the subsequent recruitment, phosphorylation, and degradation of Swe1p; autophosphorylation enhances interactions with Hsl7p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 3 | 7 | 4 | 5 | 4 | 4 | 5 | 0 | – | 0 | 0 | 1 | 3 | 6 | 5 | 1 | 7 | 7 | 4 | 5 | 1 | 4 | 9 |
Bud | 21 | 7 | 25 | 65 | 74 | 66 | 76 | 93 | 59 | – | 27 | 46 | 17 | 17 | 34 | 24 | 23 | 28 | 47 | 47 | 28 | 16 | 10 | 15 |
Bud Neck | 41 | 23 | 35 | 72 | 111 | 65 | 114 | 106 | 67 | – | 39 | 69 | 39 | 89 | 93 | 65 | 74 | 57 | 92 | 71 | 72 | 29 | 5 | 3 |
Bud Site | 15 | 18 | 29 | 31 | 58 | 36 | 50 | 33 | 19 | – | 11 | 18 | 30 | 42 | 76 | 29 | 13 | 25 | – | – | – | – | – | – |
Cell Periphery | 5 | 4 | 2 | 1 | 1 | 4 | 0 | 1 | 0 | – | 0 | 1 | 1 | 0 | 2 | 1 | 5 | 7 | 1 | 0 | 1 | 1 | 4 | 6 |
Cytoplasm | 160 | 129 | 128 | 198 | 172 | 185 | 367 | 382 | 275 | – | 141 | 231 | 134 | 157 | 162 | 209 | 211 | 292 | 94 | 74 | 69 | 229 | 403 | 446 |
Endoplasmic Reticulum | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 2 | 0 | 0 | 1 | 24 | 10 | 28 | 2 | 1 | 1 | 1 | 0 | 4 |
Endosome | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 2 | 5 | 3 | 8 | 10 | 6 | 2 | 3 | 1 | 1 | 3 | 5 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 2 |
Mitochondria | 6 | 18 | 31 | 38 | 47 | 73 | 100 | 113 | 122 | – | 94 | 135 | 3 | 5 | 18 | 21 | 18 | 17 | 8 | 10 | 19 | 7 | 14 | 36 |
Nucleus | 16 | 5 | 15 | 19 | 33 | 19 | 41 | 48 | 25 | – | 21 | 18 | 11 | 18 | 23 | 15 | 15 | 13 | 0 | 1 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | – | 1 | 0 | 0 | 3 | 4 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 5 | 1 | 6 | 4 | 6 | 12 | 11 | 11 | – | 4 | 15 | 4 | 13 | 30 | 6 | 5 | 4 | 1 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 6 | 15 | 3 | 7 | 25 | 7 | 11 | 8 | 4 | – | 3 | 2 | 20 | 23 | 38 | 18 | 26 | 27 | 2 | 0 | 2 | 0 | 0 | 0 |
SpindlePole | 36 | 9 | 18 | 34 | 103 | 49 | 111 | 96 | 32 | – | 18 | 33 | 30 | 59 | 64 | 55 | 72 | 73 | 17 | 8 | 13 | 4 | 7 | 9 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 5 | 0 | 1 | 0 | 0 | – | 0 | 2 | 0 | 3 | 5 | 5 | 7 | 5 | 2 | 8 | 5 | 3 | 6 | 6 |
Unique Cell Count | 258 | 202 | 221 | 342 | 440 | 378 | 663 | 667 | 452 | 258 | 420 | 249 | 372 | 426 | 375 | 382 | 463 | 286 | 240 | 228 | 300 | 475 | 557 | |
Labelled Cell Count | 315 | 239 | 297 | 476 | 641 | 514 | 888 | 899 | 616 | 359 | 572 | 292 | 437 | 559 | 485 | 491 | 591 | 286 | 240 | 228 | 300 | 475 | 557 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 4.3 | 3.4 | 3.2 | 3.6 | 3.1 | 3.0 | 3.1 | 2.6 | – | 2.6 | 2.4 | 4.7 | 5.3 | 4.7 | 5.2 | 5.8 | 5.7 | 4.1 | 3.5 | 4.2 |
Std Deviation (1e-4) | 1.0 | 1.4 | 1.0 | 1.2 | 1.5 | 1.0 | 1.3 | 1.2 | 1.2 | – | 0.9 | 1.1 | 1.4 | 1.7 | 1.5 | 1.3 | 1.6 | 1.6 | 1.0 | 0.9 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.12 | -0.11 | -0.18 | -0.13 | -0.36 | – | -0.38 | -0.46 | 0.5 | 0.66 | 0.49 | 0.63 | 0.78 | 0.76 | 0.28 | 0.07 | 0.31 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.2206 | 3.8612 | 4.1523 | 3.7815 | 3.6847 | 3.3494 | 0.9583 | 1.6599 | 2.413 | 2.5373 | 1.9082 | 1.3991 | 0.377 | 1.2327 | 1.8548 | 2.1578 | 1.0546 | 1.1858 |
Actin | 0.0575 | 0.0031 | 0.0036 | 0.0005 | 0.0336 | 0.0187 | 0.0242 | 0.0012 | 0.026 | 0.0574 | 0.0024 | 0.0065 | 0.0151 | 0.0009 | 0.0028 | 0.0001 | 0 | 0.0018 |
Bud | 0.0167 | 0.0081 | 0.0072 | 0.0013 | 0.0008 | 0.006 | 0.0075 | 0.0137 | 0.0035 | 0.0025 | 0.0008 | 0.0052 | 0.0317 | 0.0114 | 0.0023 | 0.0001 | 0.0001 | 0.0054 |
Bud Neck | 0.0842 | 0.0572 | 0.7037 | 0.8845 | 0.8509 | 0.464 | 0.046 | 0.0564 | 0.6914 | 0.6287 | 0.7449 | 0.3238 | 0.1298 | 0.049 | 0.6942 | 0.9944 | 0.9976 | 0.3213 |
Bud Periphery | 0.0208 | 0.0095 | 0.0068 | 0.0011 | 0.0011 | 0.0098 | 0.0084 | 0.0075 | 0.0041 | 0.0029 | 0.0008 | 0.0082 | 0.0456 | 0.0153 | 0.003 | 0.0003 | 0.0001 | 0.0042 |
Bud Site | 0.4409 | 0.5453 | 0.2144 | 0.0167 | 0.0151 | 0.0079 | 0.1451 | 0.4229 | 0.2022 | 0.0302 | 0.0112 | 0.0129 | 0.2543 | 0.5731 | 0.2299 | 0.0029 | 0.0005 | 0.0066 |
Cell Periphery | 0.0041 | 0.0055 | 0.0036 | 0.0007 | 0.0005 | 0.0016 | 0.0009 | 0.0034 | 0.0014 | 0.0011 | 0.0003 | 0.0011 | 0.0022 | 0.0033 | 0.0014 | 0.0002 | 0.0001 | 0.0006 |
Cytoplasm | 0.0243 | 0.041 | 0.0014 | 0.0002 | 0.0009 | 0.1421 | 0.0662 | 0.1243 | 0.0007 | 0.0047 | 0.0008 | 0.248 | 0.0164 | 0.0233 | 0.0008 | 0.0001 | 0.0001 | 0.1264 |
Cytoplasmic Foci | 0.045 | 0.011 | 0.0072 | 0.0179 | 0.0251 | 0.0175 | 0.1014 | 0.0076 | 0.0285 | 0.0385 | 0.072 | 0.0692 | 0.0614 | 0.0035 | 0.0042 | 0.0001 | 0.0006 | 0.0342 |
Eisosomes | 0.0017 | 0.0003 | 0.0006 | 0 | 0.0007 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0038 | 0.0015 | 0.0001 | 0 | 0.0021 | 0.0017 | 0.0041 | 0.0008 | 0.0009 | 0.0028 | 0.0002 | 0.001 | 0.002 | 0.0005 | 0.0001 | 0 | 0 | 0.0004 |
Endosome | 0.0061 | 0.0004 | 0.0022 | 0.0001 | 0.0094 | 0.0027 | 0.0371 | 0.0031 | 0.0044 | 0.0358 | 0.0314 | 0.0026 | 0.0277 | 0.0002 | 0.0043 | 0 | 0 | 0.0004 |
Golgi | 0.0044 | 0.0004 | 0.0013 | 0.0005 | 0.0055 | 0.0008 | 0.0054 | 0.0004 | 0.0075 | 0.0188 | 0.0462 | 0.0014 | 0.0099 | 0.0001 | 0.0011 | 0 | 0 | 0.0005 |
Lipid Particles | 0.0175 | 0.002 | 0.0044 | 0.0023 | 0.0117 | 0.0012 | 0.0232 | 0.001 | 0.0081 | 0.0189 | 0.0039 | 0.0019 | 0.0203 | 0.0002 | 0.0019 | 0 | 0 | 0.0014 |
Mitochondria | 0.0038 | 0.0006 | 0.0037 | 0 | 0.0046 | 0.0005 | 0.0023 | 0.0004 | 0.0038 | 0.0237 | 0.0152 | 0.0008 | 0.0026 | 0.0005 | 0.0059 | 0 | 0 | 0.0004 |
None | 0.2257 | 0.2499 | 0.0141 | 0.0001 | 0.0032 | 0.3033 | 0.3871 | 0.3082 | 0.0005 | 0.0716 | 0.0002 | 0.2854 | 0.3287 | 0.2952 | 0.0352 | 0 | 0 | 0.4766 |
Nuclear Periphery | 0.0049 | 0.0015 | 0.0005 | 0 | 0.0077 | 0.0012 | 0.0387 | 0.0004 | 0.0013 | 0.003 | 0.0001 | 0.0006 | 0.0026 | 0.0001 | 0 | 0 | 0 | 0.0002 |
Nucleolus | 0.0071 | 0.0331 | 0.0052 | 0.0109 | 0.0031 | 0.0024 | 0.0071 | 0.0178 | 0.0014 | 0.0054 | 0.0157 | 0.0016 | 0.005 | 0.0031 | 0.0017 | 0.0008 | 0.0003 | 0.0008 |
Nucleus | 0.0031 | 0.004 | 0.0018 | 0.002 | 0.0021 | 0.0027 | 0.0394 | 0.0195 | 0.0008 | 0.0028 | 0.0021 | 0.0026 | 0.0031 | 0.0028 | 0.0011 | 0.0004 | 0.0003 | 0.0012 |
Peroxisomes | 0.014 | 0.0035 | 0.0062 | 0.0279 | 0.0099 | 0.0013 | 0.0077 | 0.0008 | 0.0096 | 0.0341 | 0.0259 | 0.0037 | 0.0188 | 0.0006 | 0.0034 | 0 | 0 | 0.0127 |
Punctate Nuclear | 0.0083 | 0.0162 | 0.0102 | 0.0328 | 0.0106 | 0.0062 | 0.0404 | 0.0028 | 0.002 | 0.0042 | 0.0252 | 0.0193 | 0.0153 | 0.0151 | 0.0008 | 0.0002 | 0.0003 | 0.0044 |
Vacuole | 0.0041 | 0.0057 | 0.0017 | 0.0003 | 0.0007 | 0.0077 | 0.0049 | 0.007 | 0.0011 | 0.0112 | 0.0004 | 0.0038 | 0.0061 | 0.0017 | 0.0056 | 0.0001 | 0 | 0.0004 |
Vacuole Periphery | 0.002 | 0.0002 | 0.0002 | 0 | 0.0008 | 0.0006 | 0.0026 | 0.0005 | 0.0005 | 0.0014 | 0.0004 | 0.0004 | 0.001 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 82.0834 | 30.3034 | 11.0826 | 21.7754 | 97.4775 | 55.418 | 27.7399 | 19.5719 | 18.0811 | 97.243 |
Translational Efficiency | 0.9644 | 0.6543 | 0.3801 | 0.2509 | 0.5908 | 1.0524 | 0.5218 | 0.4305 | 0.3559 | 0.5857 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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